Abstract: A new and cost effective RP-HPLC method was
developed and validated for simultaneous analysis of non steroidal
anti inflammatory dugs Diclofenac sodium (DFS), Flurbiprofen
(FLP) and an opioid analgesic Tramadol (TMD) in advanced drug
delivery systems (Liposome and Microcapsules), marketed brands
and human plasma. Isocratic system was employed for the flow of
mobile phase consisting of 10 mM sodium dihydrogen phosphate
buffer and acetonitrile in molar ratio of 67: 33 with adjusted pH of
3.2. The stationary phase was hypersil ODS column (C18, 250×4.6
mm i.d., 5 μm) with controlled temperature of 30 C°. DFS in
liposomes, microcapsules and marketed drug products was
determined in range of 99.76-99.84%. FLP and TMD in
microcapsules and brands formulation were 99.78 - 99.94 % and
99.80 - 99.82 %, respectively. Single step liquid-liquid extraction
procedure using combination of acetonitrile and trichloroacetic acid
(TCA) as protein precipitating agent was employed. The detection
limits (at S/N ratio 3) of quality control solutions and plasma samples
were 10, 20, and 20 ng/ml for DFS, FLP and TMD, respectively.
The Assay was acceptable in linear dynamic range. All other
validation parameters were found in limits of FDA and ICH method
validation guidelines. The proposed method is sensitive, accurate and
precise and could be applicable for routine analysis in
pharmaceutical industry as well as in human plasma samples for
bioequivalence and pharmacokinetics studies.
Abstract: The Beshar River is one of the most important aquatic ecosystems in the upstream of the Karun watershed in south of Iran which is affected by point and non point pollutant sources . This study was done in order to evaluate the effects of pollutants activities on the water quality of the Beshar river and its aquatic ecosystems. This river is approximately 190 km in length and situated at the geographical positions of 51° 20´ to 51° 48´ E and 30° 18´ to 30° 52´ N it is one of the most important aquatic ecosystems of Kohkiloye and Boyerahmad province in south-west Iran. In this research project, five study stations were selected to examine water pollution in the Beshar River systems. Human activity is now one of the most important factors affecting on hydrology and water quality of the Beshar river. Humans use large amounts of resources to sustain various standards of living, although measures of sustainability are highly variable depending on how sustainability is defined. The Beshar river ecosystems are particularly sensitive and vulnerable to human activities. Therefore, to determine the impact of human activities on the Beshar River, the most important water quality parameters such as pH, dissolve oxygen (DO), Biological Oxygen Demand (BOD5), Total Dissolve Solids (TDS), Nitrates (NO3-N) and Phosphates (PO4) were estimated at the five stations. As the results show, the most important pollution index parameters such as BOD5, NO3 and PO4 increase and DO and pH decrease according to human activities (P
Abstract: Liners are made to protect the groundwater table from
the infiltration of leachate which normally carries different kinds of
toxic materials from landfills. Although these liners are engineered to
last for long period of time; unfortunately these liners fail; therefore,
toxic materials pass to groundwater. This paper focuses on the
changes of the hydraulic conductivity of a sand-bentonite liner due to
the infiltration of biofuel and ethanol fuel. Series of laboratory tests
were conducted in 20-cm-high PVC columns. Several compositions
of sand-bentonite liners were tested: 95% sand: 5% bentonite; 90%
sand: 10% bentonite; and 100% sand (passed mesh #40). The
columns were subjected to extreme pressures of 40 kPa, and 100 kPa
to evaluate the transport of alternative fuels (biofuel and ethanol
fuel). For comparative studies, similar tests were carried out using
water. Results showed that hydraulic conductivity increased due to
the infiltration of alternative fuels through the liners. Accordingly,
the increase in the hydraulic conductivity showed significant
dependency on the type of liner mixture and the characteristics of the
liquid. The hydraulic conductivity of a liner (subjected to biofuel
infiltration) consisting of 5% bentonite: 95% sand under pressure of
40 kPa and 100 kPa had increased by one fold. In addition, the
hydraulic conductivity of a liner consisting of 10% bentonite: 90%
sand under pressure of 40 kPa and 100 kPa and infiltrated by biofuel
had increased by three folds. On the other hand, the results obtained
by water infiltration under 40 kPa showed lower hydraulic
conductivities of 1.50×10-5 and 1.37×10-9 cm/s for 5% bentonite:
95% sand, and 10% bentonite: 90% sand, respectively. Similarly,
under 100 kPa, the hydraulic conductivities were 2.30×10-5 and
1.90×10-9 cm/s for 5% bentonite: 95% sand, and 10% bentonite: 90%
sand, respectively.
Abstract: The occurrence and removal of trace organic
contaminants in the aquatic environment has become a focus of
environmental concern. For the selective removal of carbamazepine
from loaded waters molecularly imprinted polymers (MIPs) were
synthesized with carbamazepine as template. Parameters varied were
the type of monomer, crosslinker, and porogen, the ratio of starting
materials, and the synthesis temperature. Best results were obtained
with a template to crosslinker ratio of 1:20, toluene as porogen, and
methacrylic acid (MAA) as monomer. MIPs were then capable to
recover carbamazepine by 93% from a 10-5 M landfill leachate
solution containing also caffeine and salicylic acid. By comparison,
carbamazepine recoveries of 75% were achieved using a nonimprinted
polymer (NIP) synthesized under the same conditions, but
without template. In landfill leachate containing solutions
carbamazepine was adsorbed by 93-96% compared with an uptake of
73% by activated carbon. The best solvent for desorption was
acetonitrile, with which the amount of solvent necessary and dilution
with water was tested. Selected MIPs were tested for their reusability
and showed good results for at least five cycles. Adsorption
isotherms were prepared with carbamazepine solutions in the
concentration range of 0.01 M to 5*10-6 M. The heterogeneity index
showed a more homogenous binding site distribution.
Abstract: This article demonstrated development of
controlled release system of an NSAID drug, Diclofenac
sodium employing different ratios of Ethyl cellulose.
Diclofenac sodium and ethyl cellulose in different proportions
were processed by microencapsulation based on phase
separation technique to formulate microcapsules. The
prepared microcapsules were then compressed into tablets to
obtain controlled release oral formulations. In-vitro evaluation
was performed by dissolution test of each preparation was
conducted in 900 ml of phosphate buffer solution of pH 7.2
maintained at 37 ± 0.5 °C and stirred at 50 rpm. At predetermined
time intervals (0, 0.5, 1.0, 1.5, 2, 3, 4, 6, 8, 10, 12,
16, 20 and 24 hrs). The drug concentration in the collected
samples was determined by UV spectrophotometer at 276 nm.
The physical characteristics of diclofenac sodium
microcapsules were according to accepted range. These were
off-white, free flowing and spherical in shape. The release
profile of diclofenac sodium from microcapsules was found to
be directly proportional to the proportion of ethylcellulose and
coat thickness. The in-vitro release pattern showed that with
ratio of 1:1 and 1:2 (drug: polymer), the percentage release of
drug at first hour was 16.91 and 11.52 %, respectively as
compared to 1:3 which is only 6.87 % with in this time. The
release mechanism followed higuchi model for its release
pattern. Tablet Formulation (F2) of present study was found
comparable in release profile the marketed brand Phlogin-SR,
microcapsules showed an extended release beyond 24 h.
Further, a good correlation was found between drug release
and proportion of ethylcellulose in the microcapsules.
Microencapsulation based on coacervation found as good
technique to control release of diclofenac sodium for making
the controlled release formulations.
Abstract: Worm propagation profiles have significantly changed
since 2003-2004: sudden world outbreaks like Blaster or Slammer
have progressively disappeared and slower but stealthier worms
appeared since, most of them for botnets dissemination. Decreased
worm virulence results in more difficult detection.
In this paper, we describe a stealth worm propagation model
which has been extensively simulated and analysed on a huge virtual
network. The main features of this model is its ability to infect any
Internet-like network in a few seconds, whatever may be its size while
greatly limiting the reinfection attempt overhead of already infected
hosts. The main simulation results shows that the combinatorial
topology of routing may have a huge impact on the worm propagation
and thus some servers play a more essential and significant role than
others. The real-time capability to identify them may be essential to
greatly hinder worm propagation.
Abstract: An experimental study is presented on the effect
of microstructural change on the Portevin-Le Chatelier effect
behaviour of Al-2.5%Mg alloy. Tensile tests are performed on
the as received and heat treated (at 400 ºC for 16 hours)
samples for a wide range of strain rates. The serrations
observed in the stress-time curve are investigated from
statistical analysis point of view. Microstructures of the
samples are characterized by optical metallography and X-ray
diffraction. It is found that the excess vacancy generated due
to heat treatment leads to decrease in the strain rate sensitivity
and the increase in the number of stress drop occurrences per
unit time during the PLC effect. The microstructural
parameters like domain size, dislocation density have no
appreciable effect on the PLC effect as far as the statistical
behavior of the serrations is considered.
Abstract: Feature selection plays an important role in applications with high dimensional data. The assessment of the stability of feature selection/ranking algorithms becomes an important issue when the dataset is small and the aim is to gain insight into the underlying process by analyzing the most relevant features. In this work, we propose a graphical approach that enables to analyze the similarity between feature ranking techniques as well as their individual stability. Moreover, it works with whatever stability metric (Canberra distance, Spearman's rank correlation coefficient, Kuncheva's stability index,...). We illustrate this visualization technique evaluating the stability of several feature selection techniques on a spectral binary dataset. Experimental results with a neural-based classifier show that stability and ranking quality may not be linked together and both issues have to be studied jointly in order to offer answers to the domain experts.
Abstract: The major source of allergy in home is the house dust
mite (Dematophagoides farina, Dermatophagoides pteronyssinus)
causing allergic symptom include atopic dermatitis, asthma, perennial
rhinitis and even infant death syndrome.
Control of this mite species is dependent on the use of chemical
methods such as fumigation treatments with methylene bromide,
spraying with organophosphates such as pirimiphos-methyl, or
treatments with repellents such as DEET and benzyl benzoate.
Although effective, their repeated use for decades has sometimes
resulted in development of resistance and fostered environmental and
human health concerns. Both decomposing animal parts and the
protein that surrounds mite fecal pellets cause mite allergy. So it is
more effective to repel than to kill them because allergen is not living
house dust mite but dead body or fecal particles of house dust mite.
It is important to find out natural repellent material against house
dust mite to control them and reduce the allergic reactions. Plants may
be an alternative source for dust mite control because they contain a
range of bioactive chemicals.
The research objectives of this paper were to verify the acaricidal
and repellent effects of cinnamon essential oil and to find out it-s most
effective concentrations. We could find that cinnamon bark essential
oil was very effective material to control the house dust mite.
Furthermore, it could reduce chemical resistance and danger for
human health.
Abstract: In this paper, a joint source-channel coding (JSCC) scheme for time-varying channels is presented. The proposed scheme uses hierarchical framework for both source encoder and transmission via QAM modulation. Hierarchical joint source channel codes with hierarchical QAM constellations are designed to track the channel variations which yields to a higher throughput by adapting certain parameters of the receiver to the channel variation. We consider the problem of still image transmission over time-varying channels with channel state information (CSI) available at 1) receiver only and 2) both transmitter and receiver being informed about the state of the channel. We describe an algorithm that optimizes hierarchical source codebooks by minimizing the distortion due to source quantizer and channel impairments. Simulation results, based on image representation, show that, the proposed hierarchical system outperforms the conventional schemes based on a single-modulator and channel optimized source coding.
Abstract: Compost manufacturing plants are one of units where
wastewater is produced in significantly large amounts. Wastewater
produced in these plants contains high amounts of substrate (organic
loads) and is classified as stringent waste which creates significant
pollution when discharged into the environment without treatment. A
compost production plant in the one of the Iran-s province treating
200 tons/day of waste is one of the most important environmental
pollutant operations in this zone. The main objectives of this paper
are to investigate the compost wastewater treatability in hybrid
anaerobic reactors with an upflow-downflow arrangement, to
determine the kinetic constants, and eventually to obtain an
appropriate mathematical model. After starting the hybrid anaerobic
reactor of the compost production plant, the average COD removal
rate efficiency was 95%.
Abstract: Extraction of Fe(III) from aqueous solution using Trin-
butyl Phosphate (TBP) as carrier needs a highly acidic medium
(>6N) as it favours formation of chelating complex FeCl3.TBP.
Similarly, stripping of Iron(III) from loaded organic solvents requires
neutral pH or alkaline medium to dissociate the same complex. It is
observed that TBP co-extracts acids along with metal, which causes
reversal of driving force of extraction and iron(III) is re-extracted
back from the strip phase into the feed phase during Liquid Emulsion
Membrane (LEM) pertraction. Therefore, rate of extraction of
different mineral acids (HCl, HNO3, H2SO4) using TBP with and
without presence of metal Fe(III) was examined. It is revealed that in
presence of metal acid extraction is enhanced. Determination of mass
transfer coefficient of both acid and metal extraction was performed
by using Bulk Liquid Membrane (BLM). The average mass transfer
coefficient was obtained by fitting the derived model equation with
experimentally obtained data. The mass transfer coefficient of the
mineral acid extraction is in the order of kHNO3 = 3.3x10-6m/s > kHCl =
6.05x10-7m/s > kH2SO4 = 1.85x10-7m/s. The distribution equilibria of
the above mentioned acids between aqueous feed solution and a
solution of tri-n-butyl-phosphate (TBP) in organic solvents have been
investigated. The stoichiometry of acid extraction reveals the
formation of TBP.2HCl, HNO3.2TBP, and TBP.H2SO4 complexes.
Moreover, extraction of Iron(III) by TBP in HCl aqueous solution
forms complex FeCl3.TBP.2HCl while in HNO3 medium forms
complex 3FeCl3.TBP.2HNO3
Abstract: Pyritisation halos are identified in weathering crusts and unconsolidated formations at five locations within large fault structure of the Urals’ eastern slope. Electron microscopy reveals the presence of inclusions and growths on pyrite faces – normally on cubic pyrite with striations, or combinations of cubes and other forms. Following neogenesis types are established: native elements and intermetallic compounds (including gold and silver), halogenides, sulphides, sulfosalts, tellurides, sulphotellurides,
selenides, tungstates, sulphates, phosphates, carbon-based substances. Direct relationship is noted between amount and diversity of such mineral phases, and proximity to and scale of ore-grade mineralization. Gold and silver, both in native form and within tellurides, presence of lead (galena, native lead), native tungsten, and, possibly, molybdenite and sulfosalts can indicate gold-bearing formations. First find of native tungsten in the Urals is for the first time – in crystallised and druse-like form. Link is suggested between unusual mineralization and “reducing” hydrothermal fluids from deep-seated faults at later stages of Urals’ reactivation.
Abstract: The nanotechnology based on epitaxial systems
includes single or arranged misfit dislocations. In general, whatever
is the type of dislocation or the geometry of the array formed by the
dislocations; it is important for experimental studies to know exactly
the stress distribution for which there is no analytical expression [1,
2]. This work, using a numerical analysis, deals with relaxation of
epitaxial layers having at their interface a periodic network of edge
misfit dislocations. The stress distribution is estimated by using
isotropic elasticity. The results show that the thickness of the two
sheets is a crucial parameter in the stress distributions and then in the
profile of the two sheets.
A comparative study between the case of single dislocation and
the case of parallel network shows that the layers relaxed better when
the interface is covered by a parallel arrangement of misfit.
Consequently, a single dislocation at the interface produces an
important stress field which can be reduced by inserting a parallel
network of dislocations with suitable periodicity.
Abstract: One of the main trouble in a steel strip manufacturing
line is the breakage of whatever weld carried out between steel coils,
that are used to produce the continuous strip to be processed. A weld
breakage results in a several hours stop of the manufacturing line. In
this process the damages caused by the breakage must be repaired.
After the reparation and in order to go on with the production it will
be necessary a restarting process of the line. For minimizing this
problem, a human operator must inspect visually and manually each
weld in order to avoid its breakage during the manufacturing process.
The work presented in this paper is based on the Bayesian decision
theory and it presents an approach to detect, on real-time, steel strip
defective welds. This approach is based on quantifying the tradeoffs
between various classification decisions using probability and the
costs that accompany such decisions.
Abstract: Phytases are acid phosphatase enzymes, which
efficiently cleave phosphate moieties from phytic acid, thereby
generating myo-inositol and inorganic phosphate. Thirty four
isolates of endophytic fungi to produce of phytases were isolated
from leaf, stem and root fragments of soybean. Screening of 34
isolates of endophytic fungi identified the phytases produced by
Rhizoctonia sp. and Fusarium verticillioides . The phytase
production were the best induced by phytic acid and rice bran
compared the others inducer in submerged fermentation medium
used. The phytase produced by both Rhizoctonia sp. and F.
verticillioides have pH optimum at 4.0 and 5.0 respectively. The
characterization of phytase from Fusarium verticillioides showed that
temperature optimum was 500C and stability until 600C, the pH
optimum 5.0 and pH stability was 2.5 – 6.0, and substrate specificity
were rice bran>soybean meal>corn> coconut cake, respectively.
Abstract: Study of fire and explosion is very important mainly
in oil and gas industries due to several accidents which have been
reported in the past and present. In this work, we have investigated
the flammability of bio oil vapour mixtures. This mixture may
contribute to fire during the storage and transportation process. Bio
oil sample derived from Palm Kernell shell was analysed using Gas
Chromatography Mass Spectrometry (GC-MS) to examine the
composition of the sample. Mole fractions of 12 selected
components in the liquid phase were obtained from the GC-FID data
and used to calculate mole fractions of components in the gas phase
via modified Raoult-s law. Lower Flammability Limits (LFLs) and
Upper Flammability Limits (UFLs) for individual components were
obtained from published literature. However, stoichiometric
concentration method was used to calculate the flammability limits
of some components which their flammability limit values are not
available in the literature. The LFL and UFL values for the mixture
were calculated using the Le Chatelier equation. The LFLmix and
UFLmix values were used to construct a flammability diagram and
subsequently used to determine the flammability of the mixture. The
findings of this study can be used to propose suitable inherently
safer method to prevent the flammable mixture from occurring and
to minimizing the loss of properties, business, and life due to fire
accidents in bio oil productions.
Abstract: The objective of this manuscript is to find area under the plasma concentration- time curve (AUC) for multiple doses of salbutamol sulphate sustained release tablets (Ventolin® oral tablets SR 8 mg, GSK, Pakistan) in the group of 18 healthy adults by using computational mathematics techniques. Following the administration of 4 doses of Ventolin® tablets 12 hourly to 24 healthy human subjects and bioanalysis of obtained plasma samples, plasma drug concentration-time profile was constructed. AUC, an important pharmacokinetic parameter, was measured using integrated equation of multiple oral dose regimens. The approximated AUC was also calculated by using computational mathematics techniques such as repeated rectangular, repeated trapezium and repeated Simpson's rule and compared with exact value of AUC calculated by using integrated equation of multiple oral dose regimens to find best computational mathematics method that gives AUC values closest to exact. The exact values of AUC for four consecutive doses of Ventolin® oral tablets were 150.5819473, 157.8131756, 164.4178231 and 162.78 ng.h/ml while the closest values approximated AUC values were 149.245962, 157.336171, 164.2585768 and 162.289224 ng.h/ml, respectively as found by repeated rectangular rule. The errors in the approximated values of AUC were negligible. It is concluded that all computational tools approximated values of AUC accurately but the repeated rectangular rule gives slightly better approximated values of AUC as compared to repeated trapezium and repeated Simpson's rules.
Abstract: The pulp and paper mill effluent is one of the high
polluting effluent amongst the effluents obtained from polluting
industries. All the available methods for treatment of pulp and paper
mill effluent have certain drawbacks. The coagulation is one of the
cheapest process for treatment of various organic effluents. Thus, the
removal of chemical oxygen demand (COD) and colour of paper mill
effluent is studied using coagulation process. The batch coagulation
process was performed using various coagulants like: aluminium
chloride, poly aluminium chloride and copper sulphate. The initial
pH of the effluent (Coagulation pH) has tremendous effect on COD
and colour removal. Poly aluminium chloride (PAC) as coagulant
reduced COD to 84 % and 92 % of colour was removed at an
optimum pH 5 and coagulant dose of 8 ml l-1. With aluminium
chloride at an optimum pH = 4 and coagulant dose of 5 g l-1, 74 %
COD and 86 % colour removal were observed. The results using
copper sulphate as coagulant (a less commercial coagulant) were
encouraging. At an optimum pH 6 and mass loading of 5 g l-1, 76 %
COD reduction and 78 % colour reduction were obtained. It was also
observed that after addition of coagulant, the pH of the effluent
decreases. The decrease in pH was highest for AlCl3, which was
followed by PAC and CuSO4. Significant amount of COD reductions
was obtained by coagulation process. Since the coagulation process
is the first stage for treatment of effluent and some of the coagulant
cations usually remain in the treated effluents. Thus, cation like
copper may be one of the good catalyst for second stage of treatment
process like wet oxidation. The copper has been found to be good
oxidation catalyst then iron and aluminum.
Abstract: The treatment of the industrial wastewater can be
particularly difficult in the presence of toxic compounds. Excessive
concentration of Chromium in soluble form is toxic to a wide variety
of living organisms. Biological removal of heavy metals using natural
and genetically engineered microorganisms has aroused great interest
because of its lower impact on the environment. Ralston
metallidurans, formerly known as Alcaligenes eutrophus is a LProteobacterium
colonizing industrial wastewater with a high content
of heavy metals. Tris-buffered mineral salt medium was used for
growing Alcaligenes eutrophus AE104 (pEBZ141). The cells were
cultivated for 18 h at 30 oC in Tris-buffered mineral salt medium
containing 3 mM disodium sulphate and 46 mM sodium gluconate as
the carbon source. The cells were harvested by centrifugation,
washed, and suspended in 10 mM Tris HCl, pH 7.0, containing 46
mM sodium gluconate, and 5 mM Chromium. Interaction among
induction of chr resistance determinant, and chromate reduction have
been demonstrated. Results of this study show that the above bacteria
can be very useful for bioremediation of chromium from industrial
wastewater.