Identification Characterization and Production of Phytase from Endophytic Fungi
Phytases are acid phosphatase enzymes, which
efficiently cleave phosphate moieties from phytic acid, thereby
generating myo-inositol and inorganic phosphate. Thirty four
isolates of endophytic fungi to produce of phytases were isolated
from leaf, stem and root fragments of soybean. Screening of 34
isolates of endophytic fungi identified the phytases produced by
Rhizoctonia sp. and Fusarium verticillioides . The phytase
production were the best induced by phytic acid and rice bran
compared the others inducer in submerged fermentation medium
used. The phytase produced by both Rhizoctonia sp. and F.
verticillioides have pH optimum at 4.0 and 5.0 respectively. The
characterization of phytase from Fusarium verticillioides showed that
temperature optimum was 500C and stability until 600C, the pH
optimum 5.0 and pH stability was 2.5 – 6.0, and substrate specificity
were rice bran>soybean meal>corn> coconut cake, respectively.
[1] J. Pallauf, and G. Rimbach. Nutritional Significance of Phytic Acid and
Phytase. Arch. Anim. Nutr. . 1996; 50: 301-319.
[2] J. A., Martin, R. A. Murphy and R. F. F. Power. Purrification and
Physico- Chemical Characterisation of Genetically Modifed Phytases
Expressed in Aspergillus awamori. Bioresource Technology 2005. pp
97.
[3] L., W. Cao, Wang., C. Yang., Y. Yang., J. Diana., A. Yakupitiyage., Z.
Luo And D. Li.. Application of Microbial Phytase in Fish Feed. J.
Enzyme and Microbial Technology. 2007 40: 497-507.
[4] Z., H. Liu, Wang., Xiu-E Wang., H. Xu., D. Gao., G. Zhang,. P. Chen
And D. Liu. Effect of Wheat Perling on Flour Phytase Activity, Phytic
Acid, Iron and Zinc Content. Food Sci and Technology. 2007.
[5] N. Takizawa.. Utilization of Recombinan Phytase on the Avoidance of
Water Pollution by Phosporus of Excretory Organ of Liverstock.
Seitbutsu-kakagu. , 1998, pp 76.
[6] R Greiner,., Haller, E., Konietzny, U. and K. D. Jany.. Purification and
Characterization of A Phytase From Klebsiella Terrigena. Arch.
Biochem. Biophys. 1997, 341: 201-206.
[7] V. K. Powar, and V. Jagannathan.. Purfication and Properties of Phytate-
Specific Phosphatase from Bacillus subtilis. J. Bacteriol. 1982, 151:
1102-1108.
[8] R,Greiner, Konietzny, U. and Jany, K. D.. Purification and
Characterization of Two Phytases From Escherichia coli. Arch.
Biochem. Biophys. 1993, 303: 107-113.
[9] S. J Yoon,., Y. J. Choi., H. K. Min., K. K Cho., J. W. Kim., S. C. Lee
and Y. H.. Jung. Isolation and Identification of Phytase-Producing
Bacterium, Enterobacter sp. 4, and Enzymatic Properties of Phytase
Enzyme. Enzyme Microbiol. Technol. 1996., 18: 449-454.
[10] V, Shah, and Parekh, L. J.. Phytase From Klebsiella sp. No. Pg-2:
Purification and Properties. Indian J. Biochem. Biophys. 1990, 27: 98-
102.
[11] S. J, Howson,. and R. J. Davis.. Production of Phytate-Hydrolysing
Enzyme by Fungi. J. Enzyme Microbiol. Technol. 1983, 5: 377-382.
[12] C., L. Quan, Zhang., Y. Wang and Y. Ohta.. Prpduction of Phytase in
Low Phosphate Medium by a Novel Yeast Candida krusei. Journal of
Bioscience and Bioenginering. 2001, 9 (2): 154-160.
[13] R. A Samson, and E. S. van Reenen-Hoekstra.. Intoduction to Food-
Borne Fungi. 1988, Centraalbureau voor Schimmelcultures.
Wageningen.
[14] H. L ,Barnett, and B. B, Hunter.. Ilustrated Genera of Imperfect Fungi.
Third Edition. 1972, Burgess Publishing Company. USA.
[15] T. W Kim, and X. G. Lei. 2005. An Improved Method for a Rapid
Determination of Phytase activity in animal feed. J. Anim. Sci. 83: 1062-
1067.
[16] McKee, Trudy,.. Biochemistry: An Intoduction. Secend Edition. 1999,
McGraw-Hill. Amerika.
[17] M ,Yetti.. Isolation and Purification of Raw Starch Degrading Enzyme
from Acremonium endophytic Fungus and Its Aplication for Glucosa
Production. Dissertation of Doctoral at University Putra Malaysia.
Malaysia. 2001.
[18] M, Yetti., Saari N., Radu S and Bakar F.A.. Raw starch degrading
enzyme from newly isolated strains of endophytic fungi. World Journal
Microbiology and Biotechnology 2000, 16.575-578.
[19] A., Boyce and Walsh.,G.. Purification and characterization of an
phosphatase with phytase activity from Mucor heimalis Wehmer.
Journal of Biotechnology 2007, 132. 82-87.
[1] J. Pallauf, and G. Rimbach. Nutritional Significance of Phytic Acid and
Phytase. Arch. Anim. Nutr. . 1996; 50: 301-319.
[2] J. A., Martin, R. A. Murphy and R. F. F. Power. Purrification and
Physico- Chemical Characterisation of Genetically Modifed Phytases
Expressed in Aspergillus awamori. Bioresource Technology 2005. pp
97.
[3] L., W. Cao, Wang., C. Yang., Y. Yang., J. Diana., A. Yakupitiyage., Z.
Luo And D. Li.. Application of Microbial Phytase in Fish Feed. J.
Enzyme and Microbial Technology. 2007 40: 497-507.
[4] Z., H. Liu, Wang., Xiu-E Wang., H. Xu., D. Gao., G. Zhang,. P. Chen
And D. Liu. Effect of Wheat Perling on Flour Phytase Activity, Phytic
Acid, Iron and Zinc Content. Food Sci and Technology. 2007.
[5] N. Takizawa.. Utilization of Recombinan Phytase on the Avoidance of
Water Pollution by Phosporus of Excretory Organ of Liverstock.
Seitbutsu-kakagu. , 1998, pp 76.
[6] R Greiner,., Haller, E., Konietzny, U. and K. D. Jany.. Purification and
Characterization of A Phytase From Klebsiella Terrigena. Arch.
Biochem. Biophys. 1997, 341: 201-206.
[7] V. K. Powar, and V. Jagannathan.. Purfication and Properties of Phytate-
Specific Phosphatase from Bacillus subtilis. J. Bacteriol. 1982, 151:
1102-1108.
[8] R,Greiner, Konietzny, U. and Jany, K. D.. Purification and
Characterization of Two Phytases From Escherichia coli. Arch.
Biochem. Biophys. 1993, 303: 107-113.
[9] S. J Yoon,., Y. J. Choi., H. K. Min., K. K Cho., J. W. Kim., S. C. Lee
and Y. H.. Jung. Isolation and Identification of Phytase-Producing
Bacterium, Enterobacter sp. 4, and Enzymatic Properties of Phytase
Enzyme. Enzyme Microbiol. Technol. 1996., 18: 449-454.
[10] V, Shah, and Parekh, L. J.. Phytase From Klebsiella sp. No. Pg-2:
Purification and Properties. Indian J. Biochem. Biophys. 1990, 27: 98-
102.
[11] S. J, Howson,. and R. J. Davis.. Production of Phytate-Hydrolysing
Enzyme by Fungi. J. Enzyme Microbiol. Technol. 1983, 5: 377-382.
[12] C., L. Quan, Zhang., Y. Wang and Y. Ohta.. Prpduction of Phytase in
Low Phosphate Medium by a Novel Yeast Candida krusei. Journal of
Bioscience and Bioenginering. 2001, 9 (2): 154-160.
[13] R. A Samson, and E. S. van Reenen-Hoekstra.. Intoduction to Food-
Borne Fungi. 1988, Centraalbureau voor Schimmelcultures.
Wageningen.
[14] H. L ,Barnett, and B. B, Hunter.. Ilustrated Genera of Imperfect Fungi.
Third Edition. 1972, Burgess Publishing Company. USA.
[15] T. W Kim, and X. G. Lei. 2005. An Improved Method for a Rapid
Determination of Phytase activity in animal feed. J. Anim. Sci. 83: 1062-
1067.
[16] McKee, Trudy,.. Biochemistry: An Intoduction. Secend Edition. 1999,
McGraw-Hill. Amerika.
[17] M ,Yetti.. Isolation and Purification of Raw Starch Degrading Enzyme
from Acremonium endophytic Fungus and Its Aplication for Glucosa
Production. Dissertation of Doctoral at University Putra Malaysia.
Malaysia. 2001.
[18] M, Yetti., Saari N., Radu S and Bakar F.A.. Raw starch degrading
enzyme from newly isolated strains of endophytic fungi. World Journal
Microbiology and Biotechnology 2000, 16.575-578.
[19] A., Boyce and Walsh.,G.. Purification and characterization of an
phosphatase with phytase activity from Mucor heimalis Wehmer.
Journal of Biotechnology 2007, 132. 82-87.
@article{"International Journal of Biological, Life and Agricultural Sciences:62402", author = "Yetti Marlida and Rina Delfita and Neni Gusmanizar and Gita Ciptaan", title = "Identification Characterization and Production of Phytase from Endophytic Fungi", abstract = "Phytases are acid phosphatase enzymes, which
efficiently cleave phosphate moieties from phytic acid, thereby
generating myo-inositol and inorganic phosphate. Thirty four
isolates of endophytic fungi to produce of phytases were isolated
from leaf, stem and root fragments of soybean. Screening of 34
isolates of endophytic fungi identified the phytases produced by
Rhizoctonia sp. and Fusarium verticillioides . The phytase
production were the best induced by phytic acid and rice bran
compared the others inducer in submerged fermentation medium
used. The phytase produced by both Rhizoctonia sp. and F.
verticillioides have pH optimum at 4.0 and 5.0 respectively. The
characterization of phytase from Fusarium verticillioides showed that
temperature optimum was 500C and stability until 600C, the pH
optimum 5.0 and pH stability was 2.5 – 6.0, and substrate specificity
were rice bran>soybean meal>corn> coconut cake, respectively.", keywords = "endophytic fungus, phytase, soybean, Rhizoctoniasp., Fusarium verticillioides,", volume = "4", number = "5", pages = "363-4", }
