Abstract: EcoDam is an adenine-N6 DNA methyltransferase
that methylates the GATC sites in the Escherichia coli genome.
DNA-adenine methylation is not present in higher eukaryotes
including humans. These observations raise the possibility that dam
inhibitors may be used as anti-microbial agents. Polyphosphate
(Poly(P)) is an important metabolite and signaling molecule in
prokaryotes and eukaryotes. Here, by using gel retardation
experiments to investigate the competition of DNA binding by
EcoDam in the presence of polyphosphate, we found that Poly (P)
strongly interferes with DNA binding by EcoDam, while same
concentration of monophosphate does not. In addition, we
demonstrated that Poly (P) binding inhibits the activity of EcoDam
and our results suggest that Poly (P) led to strong inhibition of the
EcoDam catalytic activity, while monophosphate had only moderate
effect.
Abstract: Stormwater wetlands have been mainly designed in an
empirical approach for water quality improvement, with little
quantitative understanding of the internal microbial processes. This
study investigated into heterotrophic bacterial production rate,
heterotrophic bacterial mineralization percentage, and algal biomass
in hypertrophic and eutrophic surface flow stormwater wetlands.
Compared to a nearby wood leachate treatment wetland, the
stormwater wetlands had much higher chlorophyll-a concentrations.
The eutrophic stormwater wetland had improved water quality,
whereas the hypertrophic stormwater wetland had degraded water
quality. Heterotrophic bacterial activities in water were limited in the
stormwater wetlands due to competition of algal growth for nutrients.
The relative contribution of biofilms to the overall heterotrophic
activities was higher in the stormwater wetlands than that in the wood
leachate treatment wetland.
Abstract: The PRAF family of proteins is a plant specific family of proteins with distinct domain architecture and various unique sequence/structure traits. We have carried out an extensive search of the Arabidopsis genome using an automated pipeline and manual methods to verify previously known and identify unknown instances of PRAF proteins, characterize their sequence and build 3D structures of their individual domains. Integrating the sequence, structure and whatever little known experimental details for each of these proteins and their domains, we present a comprehensive characterization of the different domains in these proteins and their variant properties.
Abstract: Absorption and fluorescence spectra of quinine
sulphate (QSD) have been recorded at room temperature in wide
range of solvents of different polarities. The ground-state dipole
moment of QSD was obtained from quantum mechanical calculations
and the excited state dipole moment of QSD was estimated from
Bakhshiev-s and Kawski-Chamma-Viallet-s equations by means of
solvatochromic shift method. Higher value of dipole moment is
observed for excited state as compared to the corresponding ground
state value and this is attributed to the more polar excited state of
QSD.
Abstract: Software Development Risks Identification (SDRI),
using Fault Tree Analysis (FTA), is a proposed technique to identify
not only the risk factors but also the causes of the appearance of the
risk factors in software development life cycle. The method is based
on analyzing the probable causes of software development failures
before they become problems and adversely affect a project. It uses
Fault tree analysis (FTA) to determine the probability of a particular
system level failures that are defined by A Taxonomy for Sources of
Software Development Risk to deduce failure analysis in which an
undesired state of a system by using Boolean logic to combine a
series of lower-level events. The major purpose of this paper is to use
the probabilistic calculations of Fault Tree Analysis approach to
determine all possible causes that lead to software development risk
occurrence
Abstract: The aim of this paper is description of the notion of
the death for prisoners and the ways of deal with. They express
indifference, coldness, inability to accept the blame, they have no
shame and no empathy. Is it enough to perform acts verging on the
death. In this paper we described mechanisms and regularities of selfdestructive
behaviour in the view of the relevant literature? The
explanation of the phenomenon is of a biological and sociopsychological
nature. It must be clearly stated that all forms of selfdestructive
behaviour result from various impulses, conflicts and
deficits. That is why they should be treated differently in terms of
motivation and functions which they perform in a given group of
people. Behind self-destruction there seems to be a motivational
mechanism which forces prisoners to rebel and fight against the hated
law and penitentiary systems. The imprisoned believe that pain and
suffering inflicted on them by themselves are better than passive
acceptance of repression. The variety of self-destruction acts is wide,
and some of them take strange forms. We assume that a life-death
barrier is a kind of game for them. If they cannot change the
degrading situation, their life loses sense.
Abstract: Numerical study of a plane jet occurring in a vertical
heated channel is carried out. The aim is to explore the influence of
the forced flow, issued from a flat nozzle located in the entry section
of a channel, on the up-going fluid along the channel walls. The
Reynolds number based on the nozzle width and the jet velocity
ranges between 3 103 and 2.104; whereas, the Grashof number based
on the channel length and the wall temperature difference is 2.57
1010. Computations are established for a symmetrically heated
channel and various nozzle positions. The system of governing
equations is solved with a finite volumes method. The obtained
results show that the jet-wall interactions activate the heat transfer,
the position variation modifies the heat transfer especially for low
Reynolds numbers: the heat transfer is enhanced for the adjacent
wall; however it is decreased for the opposite one. The numerical
velocity and temperature fields are post-processed to compute the
quantities of engineering interest such as the induced mass flow rate,
and the Nusselt number along the plates.
Abstract: The present work was conducted to find out the effect
of biofertilizer formulated with four species of bacteria (two species
of Azotobacter and two species of Lysobacter) and zinc sulphate.
Field experiments with mustard plant were conducted to study the
effectiveness of soil application of zinc sulphate and biofertilizer at
0, 10, 20, 30, 40, 50 days after sowing. Plant height and condition of
plant was found to be increased significantly using a mixture of
biofertilizer and zinc sulphate than other treatments after 40 days
sowing. Three treatments were also used in this field experiment such
as bacteria only, zinc sulphate only and mixture of biofertilizer and
zinc sulphate. The treatment using a mixture of zinc sulphate and
biofertilizer had the best yield (4688.008 kg/ha) within 50 days of
sowing and performed better than other treatments. Field experiment
using zinc sulphate only was second best yield (3380.75Kg/ha) and
biofertilizer only treatment gave (2639.04kg/ha).
Abstract: Column leach test has been performed to examine the
behavior of leaching of sodium, calcium and potassium in landfills.
In the column leach apparatus, two different layers of contaminated
and uncontaminated soils of different height ratios (ratio of depth of
contaminated soil to the depth of uncontaminated soil) are taken.
Water is poured from an overhead tank at a particular flowrate to the
inlet of the soil column for a certain ponding depth over the
contaminated soil. Subsequent infiltration causes leaching and the
leachates are collected from the bottom of the column. The
concentrations of Na, Ca and K in the leachate are measured using
flame photometry. The experiments are further extended by changing
the rates of flow from the overhead tank to the inlet of the column in
achieving the same ponding depth. The experiments are performed
for different scenarios in which the height ratios are altered and the
variations of concentrations of Na, Ca, and K are observed. The study
brings an estimation of leaching in landfill sites for different heights
and precipitation intensity where a ponding depth is maintained over
the landfill. It has been observed that the leaching behavior of Na,
Ca, and K are not similar. Calcium exhibits highest amount of
leaching compared to Sodium and Potassium under similar
experimental conditions.
Abstract: Sampling and analysis of leachate from Bhalaswa
landfill and groundwater samples from nearby locations, clearly
indicated the likely contamination of groundwater due to landfill
leachate. The results of simulation studies carried out for the
migration of Chloride from landfill shows that the simulation results
are in consonance with the observed concentration of Chloride in the
vicinity of landfill facility. The solid waste disposal system presently
being practiced in Delhi consists of mere dumping of wastes
generated, at three locations Bhalaswa, Ghazipur, and Okhla without
any regard to proper care for the protection of surrounding
environment. Bhalaswa landfill site in Delhi, which is being operated
as a dump site, is expected to become cause of serious groundwater
pollution in its vicinity. The leachate from Bhalaswa landfill was
found to be having a high concentration of chlorides, as well as DOC,
COD. The present study was undertaken to determine the likely
concentrations of principle contaminants in the groundwater over a
period of time due to the discharge of such contaminants from
landfill leachates to the underlying groundwater. The observed
concentration of chlorides in the groundwater within 75m of the
radius of landfill facility was found to be in consonance with the
simulated concentration of chloride in groundwater considering one
dimensional transport model, with finite mass of contaminant source.
Governing equation of contaminant transport involving advection and
diffusion-dispersion was solved in matlab7.0 using finite difference
method.
Abstract: The aim of this study is to describe the associations
between the temperamental traits and the narrative emotional
expression. The Temperament Questionnaire was used: The FCB-TI
of Zawadzki & Strelau. A sample of 85 persons described three
emotional situations: love. hate, and anxiety. This study analyzes the
verbal form of expression by means of a written account of
emotions. The relationship between the narratives of love, hate and
anxiety and temperament characteristics were studied. Results
indicate that vigorousness (VI), perseverance (PE), sensory
sensitivity (SS), emotional reactivity (ER), endurance (EN) and
activeness (AC) have a significant impact on the emotional
expression in narratives. The temperamental traits are linked to the
form of emotional language. It means that temperament has an
impact on cognitive representations of emotions.
Abstract: This report focus on phase behavior of polyethylene glycol (PEG)4000/ phosphate/ guanidine hydrochloride/ water system at different guanidine hydrochloride concentrations and pH. The binodal of the systems was displaced toward higher concentrations of the components with increasing guanidine hydrochloride concentrations. The partition coefficient of guanidine hydrochloride was near unity and increased with decreasing pH and increasing PEG/salt (%w/w) ratio.
Abstract: The objective of the present study was to evaluate the
potential of hollow microneedles for enhancing the transdermal
delivery of Bovine Serum Albumin (MW~66,000 Da)-Fluorescein
Isothiocyanate (BSA-FITC) conjugate, a hydrophilic large molecular
compound. Moreover, the effect of different formulations was
evaluated. The series of binary mixtures composed of propylene
glycol (PG) and pH 7.4 phosphate buffer solution (PBS) was
prepared and used as a medium for BSA-FITC. The results showed
that there was no permeation of BSA-FITC solution across the
neonatal porcine skin without using hollow microneedles, whereas
the cumulative amount of BSA-FITC released at 8 h through the
neonatal porcine skin was about 60-70% when using hollow
microneedles. Furthermore, the results demonstrated that the higher
volume of PG in binary mixtures injected, the lower cumulative
amount of BSA-FITC released and release rate of BSA-FITC from
skin. These release profiles of BSA-FITC in binary mixtures were
expressed by Fick-s law of diffusion. These results suggest the
utilization of hollow microneedle to enhance transdermal delivery of
protein and provide useful information for designing an effective
hollow microneedle system.
Abstract: The spores of entomopathogenic fungi, Beauveria bassiana was evaluated for their compatibility with four surfactants; SDS (sodium dodyl sulphate) and CABS-65 (calcium alkyl benzene sulphonate), Tween 20 (polyethylene sorbitan monolaureate) and Tween 80 (polyoxyethylene sorbitan monoleate) at six different concentrations (0.1%, 0.5%, 1%, 2.5%, 5% and 10%). Incubated spores showed decrease in concentrations due to conversion of spores to hyphae. The maximum germination recorded in 72 h incubated spores varied with surfactant concentration at 49-68% (SDS), 39- 53% (CABS), 78-92% (Tween 80) and 80-92% (Tween 20), while the optimal surfactant concentration for spore germination was found to be 2.5-5%. The surfactant effect on spores was more pronounced with SDS and CABS-65, where significant deterioration and loss in viability of the incubated spores was observed. The effect of Tween 20 and Tween 80 were comparatively less inhibiting. The results of the study would help in surfactant selection for B. bassiana emulsion preparation.
Abstract: In order to evaluate the relationship between the sulphur (S), glucose (G), nitrogen (N) and plant residues (st), sulphur immobilization and microbial transformation were monitored in five soil samples from 0-30 cm of Bastam farmers fields of Shahrood area following 11 treatments with different levels of Sulphur (S), glucose (G), N and plant residues (wheat straw) in a randomized block design with three replications and incubated over 20, 45 and 60 days, the immobilization of SO4 -2-S presented as a percentage of that added, was inversely related to its addition rate. Additions of glucose and plant residues increased with the C-to-S ratio of the added amendments, irrespective of their origins (glucose and plant residues). In the presence of C sources (glucose or plant residues). N significantly increased the immobilization of SO4 -2-S, whilst the effect of N was insignificant in the absence of a C amendment. In first few days the amounts of added SO4 -2-S immobilized were linearly correlated with the amounts of added S recovered in the soil microbial biomass. With further incubation the proportions of immobilized SO4 -2-S remaining as biomass-S decreased. Decrease in biomass-S was thought to be due to the conversion of biomass-S into soil organic-S. Glucose addition increased the immobilization (microbial utilization and incorporation into the soil organic matter) of native soil SO4 -2-S. However, N addition enhance the mineralization of soil organic-S, increasing the concentration of SO4 - 2-S in soil.
Abstract: This paper introduces application of multi degree of freedom fuzzy(MDOFF) controller in permanent magnet (PM)drive system. The drive system model is developed for FO control. Simulation of the system is carried out to predict the performance at NL and under load,. The results indicate that application of MDOFF controller is effective for sensorless PM drive system.
Abstract: The main objective of this research was to investigate
the biosorption capacity for biofilms of sulphate reducing bacteria
(SRB) to remove heavy metals, such as Zn, Pb and Cd from
rainwater using laboratory-scale reactors containing mixed support
media. Evidence showed that biosorption had contributed to removal
of heavy metals including Zn, Pb and Cd in presence of SRB and
SRB were also found in the aqueous samples from reactors.
However, the SRB and specific families (Desulfobacteriaceae and
Desulfovibrionaceae) were found mainly in the biomass samples
taken from all reactors at the end of the experiment. EDX-analysis
of reactor solids at end of experiment showed that heavy metals Zn,
Pb and Cd had also accumulated in these precipitates.
Abstract: In this study, the possibility of using potato as a
substrate for glutamic acid production by Brevibacterium linens
was investigated. For preparation of fermentation medium, potato
was hydrolyzed by hydrochloridric acid. The medium contained
potato hydrolysate, tween 80, mineral solution, glucose, and
potassium hydrogen phosphate. The initial pH of the medium was
adjusted to 7-7.5. For achieving the optimum time with maximum
yield, the beakers containing the medium and the inoculums were
incubated in a rotary water bath flask shaker for one to five days.
Thin layer choromatography was used for quantitative and
qualitative assay of the glutamic acid produced. The results
revealed that as fermentation time increased, pH of the
fermentation medium significantly decreased (P
Abstract: Basel III (or the Third Basel Accord) is a global
regulatory standard on bank capital adequacy, stress testing and
market liquidity risk agreed upon by the members of the Basel
Committee on Banking Supervision in 2010-2011, and scheduled to
be introduced from 2013 until 2018. Basel III is a comprehensive set
of reform measures. These measures aim to; (1) improve the banking
sector-s ability to absorb shocks arising from financial and economic
stress, whatever the source, (2) improve risk management and
governance, (3) strengthen banks- transparency and disclosures.
Similarly the reform target; (1) bank level or micro-prudential,
regulation, which will help raise the resilience of individual banking
institutions to periods of stress. (2) Macro-prudential regulations,
system wide risk that can build up across the banking sector as well
as the pro-cyclical implication of these risks over time. These two
approaches to supervision are complementary as greater resilience at
the individual bank level reduces the risk system wide shocks.
Macroeconomic impact of Basel III; OECD estimates that the
medium-term impact of Basel III implementation on GDP growth is
in the range -0,05 percent to -0,15 percent per year. On the other hand
economic output is mainly affected by an increase in bank lending
spreads as banks pass a rise in banking funding costs, due to higher
capital requirements, to their customers. Consequently the estimated
effects on GDP growth assume no active response from monetary
policy. Basel III impact on economic output could be offset by a
reduction (or delayed increase) in monetary policy rates by about 30
to 80 basis points. The aim of this paper is to create a framework
based on the recent regulations in order to prevent financial crises.
Thus the need to overcome the global financial crisis will contribute
to financial crises that may occur in the future periods. In the first
part of the paper, the effects of the global crisis on the banking
system examine the concept of financial regulations. In the second
part; especially in the financial regulations and Basel III are analyzed.
The last section in this paper explored the possible consequences of
the macroeconomic impacts of Basel III.
Abstract: Using steelmaking slag as a raw material, aragonite superstructure product had been synthesized via an indirect CO2 mineral sequestration rout. It mainly involved two separate steps, in which the element of calcium is first selectively leached from steelmaking slag by a novel leaching media consisting of organic solvent Tributyl phosphate (TBP), acetic acid, and ultra-purity water, followed by enhanced carbonation in a separate step for aragonite superstructure production as well as efficiency recovery of leaching media. Based on the different leaching medium employed in the steelmaking slag leaching process, two typical products were collected from the enhanced carbonation step. The products were characterized by X-ray powder diffraction (XRD) and scanning electron microscopy (SEM), respectively. It reveals that the needle-like aragonite crystals self-organized into aragonite superstructure particles including aragonite microspheres as well as dumbbell-like spherical particles, can be obtained from the steelmaking slag with the purity over 99%.