Abstract: Comparing other methods of waste water treatment,
constructed wetlands are one of the most fascinating practices
because being a natural process they are eco-friendly have low
construction and maintenance cost and have considerable capability
of wastewater treatment. The current research was focused mainly on
comparison of Ranunculus muricatus and Typha latifolia as wetland
plants for domestic wastewater treatment by designing and
constructing efficient pilot scale horizontal subsurface flow
mesocosms. Parameters like chemical oxygen demand, biological
oxygen demand, phosphates, sulphates, nitrites, nitrates, and
pathogenic indicator microbes were studied continuously with
successive treatments. Treatment efficiency of the system increases
with passage of time and with increase in temperature. Efficiency of
T. latifolia planted setups in open environment was fairly good for
parameters like COD and BOD5 which was showing reduction up to
82.5% for COD and 82.6% for BOD5 while DO was increased up to
125%. Efficiency of R. muricatus vegetated setup was also good but
lowers than that of T. latifolia planted showing 80.95% removal of
COD and BOD5. Ranunculus muricatus was found effective in
reducing bacterial count in wastewater. Both macrophytes were
found promising in wastewater treatment.
Abstract: The work studied the effect of germination on
proximate, phenol and flavonoid content and antioxidant activities
(AOA) of African Yam been (AYB). Germination was done in
controlled dark chamber (100% RH, 28oC). The proximate, phenol
and flavonoid content and antioxidant activities before and after
germination were investigated. The crude protein, moisture, and
crude fiber content of germinated AYB were significantly higher
(P
Abstract: Sound exposure effects have been investigated by
broadcasting a group of broilers with sound of Quran verses (Group
B) whereas the other group is the control broilers (Group C). The
growth rate comparisons in terms of weight and raw meat texture
measured by shear force have been investigated. Twenty-seven
broilers were randomly selected from each group on Day 24 and
weight measurement was carried out every week till the harvest day
(Day 39).Group B showed a higher mean weight on Day 24 (1.441 ±
0.013 kg) than Group C. Significant difference in the weight on Day
39 existed for Group B compared to Group C (p < 0.05). However,
there was no significant (p >0.05) difference of shear force in the
same muscles (breast and drumstick raw meat) of both groups but the
shear force of the breast meat for Group B and C broilers was lower
(p < 0.05) than that of their drumstick meat. Thus, broadcasting the
sound of Quran verses in the coop can be applied to improve the
growth rate of broilers for producing better quality poultry.
Abstract: The aim of this study was to select the best strains of
Saccharomyces cerevisiae able to resist lead and cadmium. Ten
strains were screened on the basis of their resistance at different
concentrations of 0, 2, 4, 8, 12, 16, 20 and 24 ppm for Pb and 0, 0.5,
1, 2, 4, 6, 8 and 10 ppm for Cd. The properties of baker's yeast
quality were decreased by the increase of Pb or Cd in growth
medium. The slope values of yield, total viable cells and gassing
power of produced baker's yeast were investigated as an indicator of
metal resistant. In addition, concentrations of Pb and Cd in produced
baker's yeast were determined. The strain of S. cerevisiae FH-620
had the highest resistance against Pb and Cd and had the minimum
levels of both two investigated metals in produced baker's yeast.
Abstract: Agriculture is the backbone of economy of Pakistan
and cotton is the major agricultural export and supreme source of raw
fiber for our textile industry. To combat severe problems of insect
and weed, combination of three genes namely Cry1Ac, Cry2A and
EPSPS genes was transferred in locally cultivated cotton variety
MNH-786 with the use of Agrobacterium mediated genetic
transformation. The present study focused on the molecular screening
of transgenic cotton plants at T3 generation in order to confirm
integration and expression of all three genes (Cry1Ac, Cry2A and
EPSP synthase) into the cotton genome. Initially, glyphosate spray
assay was used for screening of transgenic cotton plants containing
EPSP synthase gene at T3 generation. Transgenic cotton plants which
were healthy and showed no damage on leaves were selected after 07
days of spray. For molecular analysis of transgenic cotton plants in
the laboratory, the genomic DNA of these transgenic cotton plants
were isolated and subjected to amplification of the three genes. Thus,
seventeen out of twenty (Cry1Ac gene), ten out of twenty (Cry2A
gene) and all twenty (EPSP synthase gene) were produced positive
amplification. On the base of PCR amplification, ten transgenic plant
samples were subjected to protein expression analysis through
ELISA. The results showed that eight out of ten plants were actively
expressing the three transgenes. Real-time PCR was also done to
quantify the mRNA expression levels of Cry1Ac and EPSP synthase
gene. Finally, eight plants were confirmed for the presence and active
expression of all three genes at T3 generation.
Abstract: Biofuels production has come forth as a future
technology to combat the problem of depleting fossil fuels. Bio-based
ethanol production from enzymatic lignocellulosic biomass
degradation serves an efficient method and catching the eye of
scientific community. High cost of the enzyme is the major obstacle
in preventing the commercialization of this process. Thus main
objective of the present study was to optimize composition of
medium components for enhancing cellulase production by newly
isolated strain of Bacillus tequilensis. Nineteen factors were taken
into account using statistical Plackett-Burman Design. The significant
variables influencing the cellulose production were further employed
in statistical Response Surface Methodology using Central
Composite Design for maximizing cellulase production. The
optimum medium composition for cellulase production was: peptone
(4.94 g/L), ammonium chloride (4.99 g/L), yeast extract (2.00 g/L),
Tween-20 (0.53 g/L), calcium chloride (0.20 g/L) and cobalt chloride
(0.60 g/L) with pH 7, agitation speed 150 rpm and 72 h incubation at
37oC. Analysis of variance (ANOVA) revealed high coefficient of
determination (R2) of 0.99. Maximum cellulase productivity of 11.5
IU/ml was observed against the model predicted value of 13 IU/ml.
This was found to be optimally active at 60oC and pH 5.5.
Abstract: This study evaluated the use of raw or processed
Prosopis juliflora (Meskit) pods as a major ingredient in a formulated
ration to provide an alternative non-conventional concentrate for
livestock feeding in Oman. Dry Meskit pods were reduced to lengths
of 0.5- 1.0 cm to ensure thorough mixing into three diets. Meskit
pods were subjected to two types of treatments; roasting and soaking.
They were roasted at 150оC for 30 minutes using a locally-made
roasting device (40 kg barrel container rotated by electric motor and
heated by flame gas cooker). Chopped pods were soaked in tap water
for 24 hours and dried for 2 days under the sun with frequent turning.
The Meskit-pod-based diets (MPBD) were formulated and pelleted
from 500 g/kg ground Meskit pods, 240 g/kg wheat bran, 200 g/kg
barley grain, 50 g/kg local dried sardines and 10 g/kg of salt. Twenty
four 10 months-old intact Omani male lambs with average body
weight of 27.3 kg (± 0.5 kg) were used in a feeding trial for 84 days.
They were divided (on body weight basis) and allocated to four diet
combination groups. These were: Rhodes grass hay (RGH) plus a
general ruminant concentrate (GRC); RGH plus raw Meskit pods
(RMP) based concentrate; RGH plus roasted Meskit pods (ROMP)
based concentrate; RGH plus soaked Meskit pods (SMP) based
concentrate Daily feed intakes and bi-weekly body weights were
recorded. MPBD had higher contents of crude protein (CP), acid
detergent fibre (ADF) and neutral detergent fibre (NDF) than the
GRC. Animals fed various types of MPBD did not show signs of ill
health. There was a significant effect of feeding ROMP on the
performance of Omani sheep compared to RMP and SMP. The
ROMP fed animals had similar performance to those fed the GRC in
terms of feed intake, body weight gain and feed conversion ratio
(FCR).This study indicated that roasted Meskit pods based diet may
be used instead of the commercial concentrate for feeding Omani
sheep without adverse effects on performance. It offers a cheap
alternative source of protein and energy for feeding Omani sheep.
Also, it might help in solving the spread impact of Meskit trees,
maintain the ecosystem and helping in preserving the local tree
species.
Abstract: Eucalyptus species are well reputed for their
traditional use in Asia as well as in other parts of the world; therefore,
the present study was designed to investigate the antimicrobial and
antioxidant activities associated with essential oils from different
Eucalyptus species. Essential oils from the leaves of six Eucalyptus
species, including: Eucalyptus woodwardi, Eucalyptus stricklandii,
Eucalyptus salubris, Eucalyptus sargentii, Eucalyptus torquata and
Eucalyptus wandoo were separated by hydrodistillation and dried
over anhydrous sodium sulphate. DPPH, ferric reducing antioxidant
power, and hydroxyl radical scavenging activity assays were carried
out to evaluate the antioxidant potential of the oils. The results
indicate that examined oils exhibit substantial antioxidant activities
relative to ascorbic acid. Previously, these oils were evaluated for
their antimicrobial activities, against wide range of bacterial and
fungal strains, and they were shown to possess significant
antimicrobial activities. In this study, further investigation into the
growth kinetics of oil-treated microbial cultures was conducted. The
results clearly demonstrate that the microbial growth was markedly
inhibited when treated with sub-MIC concentrations of the oils.
Taken together, the results obtained indicate a high potential of the
examined essential oils as bioactive oils, for nutraceutical and
medical applications, possessing significant antioxidant and anti
microbial activities.
Abstract: Bacterial strains capable of degradation of malathion
from the domestic sewage were isolated by an enrichment culture
technique. Three bacterial strains were screened and identified as
Acinetobacter baumannii (AFA), Pseudomonas aeruginosa (PS1),
and Pseudomonas mendocina (PS2) based on morphological,
biochemical identification and 16S rRNA sequence analysis.
Acinetobacter baumannii AFA was the most efficient malathion
degrading bacterium, so used for further biodegradation study. AFA
was able to grow in mineral salt medium (MSM) supplemented with
malathion (100 mg/l) as a sole carbon source, and within 14 days,
84% of the initial dose was degraded by the isolate measured by high
performance liquid chromatography. Strain AFA could also degrade
other organophosphorus compounds including diazinon, chlorpyrifos
and fenitrothion. The effect of different culture conditions on the
degradation of malathion like inoculum density, other carbon or
nitrogen sources, temperature and shaking were examined.
Degradation of malathion and bacterial cell growth were accelerated
when culture media were supplemented with yeast extract, glucose
and citrate. The optimum conditions for malathion degradation by
strain AFA were; an inoculum density of 1.5x 10^12CFU/ml at 30°C
with shaking. A specific polymerase chain reaction primers were
designed manually using multiple sequence alignment of the
corresponding carboxylesterase enzymes of Acinetobacter species.
Sequencing result of amplified PCR product and phylogenetic
analysis showed low degree of homology with the other
carboxylesterase enzymes of Acinetobacter strains, so we suggested
that this enzyme is a novel esterase enzyme. Isolated bacterial strains
may have potential role for use in bioremediation of malathion
contaminated.
Abstract: The present study was carried out to evaluate the
effect of different levels of dietary seed and extract of Harmal
(Peganum harmala L.) on immunity of broiler chicks. A total of 350
one-day old broiler chicks (Ross 308) were randomly allocated to
five dietary treatments with four replicates pen of 14 birds each.
Dietary treatments consisted of control, 1 and 2 g/kg Harmal seed in
diet, 100 and 200 mg/L Harmal seed extract in water. Broilers
received dietary treatments from 1 to 42 d. Two birds from each pen
were randomly weighed and sacrificed at 42 d of age, the relative
weight of lymphoid organs (bursa of Fabercius and spleen) to live
weight were calculated. Antibody titers against Newcastle and
influenza viruses and sheep red blood cell were measured at 30 d of
age. Results showed that the relative weights of lymphoid organs
were not affected by dietary treatments. Furthermore, antibody titer
against Newcastle and influenza viruses as well as sheep red blood
cell antigen were significantly (P
Abstract: To date, one of the few comprehensive indicators for
the measurement of food security is the Global Food Security Index
(GFSI). This index is a dynamic quantitative and qualitative
benchmarking model, constructed from 28 unique indicators, that
measures drivers of food security across both developing and
developed countries. Whereas the GFSI has been calculated across a
set of 109 countries, in this paper we aim to present and compare, for
the Middle East and North Africa (MENA), 1) the Food Security
Index scores achieved and 2) the data available on affordability,
availability, and quality of food. The data for this work was taken
from the latest available report published by the creators of the GFSI,
which in turn used information from national and international
statistical sources. MENA countries rank from place 17/109 (Israel,
although with resent political turmoil this is likely to have changed)
to place 91/109 (Yemen) with household expenditure spent in food
ranging from 15.5% (Israel) to 60% (Egypt). Lower spending on food
as a share of household consumption in most countries and better
food safety net programs in the MENA have contributed to a notable
increase in food affordability. The region has also, however,
experienced a decline in food availability, owing to more limited
food supplies and higher volatility of agricultural production. In
terms of food quality and safety the MENA has the top ranking
country (Israel). The most frequent challenges faced by the countries
of the MENA include public expenditure on agricultural research and
development as well as volatility of agricultural production. Food
security is a complex phenomenon that interacts with many other
indicators of a country’s wellbeing; in the MENA it is slowly but
markedly improving.
Abstract: A strain of Monascus purpureus CMU001 was used to
prepare red yeast rice from Thai glutinous rice Korkor 6 (RD 6).
Adding of different amounts of histidine (156, 312, 625 and 1250 mg
in 100 g of rice grains)) under aerobic and air limitation (air-lock)
condition were used in solid fermentation. Determination of the yield
as well as monacolin K content was done. Citrinin content was also
determined in order to confirm the safety use of prepared red yeast
rice. It was found that under air-lock condition with 1250 mg of
histidine addition gave the highest yield of 37.40 g of dried red yeast
rice prepared from 100 g of rice. Highest 5.72 mg content of
monacolin K was obtained under air-lock condition with 312 mg
histidine addition. In the other hand, citrinin content was found to be
less than 24462 ng/g of all dried red yeast rice samples under the
experimental methods used in this work.
Abstract: Food as perishable goods represents a specific and
sensitive part in the supply chain theory, since changing physical or
chemical characteristics considerably influence the approach to stock
management. The most delicate phase of this process is
transportation, where it becomes difficult to ensure the stable
conditions which limit deterioration, since the value of the
deterioration rate could be easily influenced by the mode of
transportation. The fuzzy definition of variables allows one to take
these variations into account. Furthermore, an appropriate choice of
the defuzzification method permits one to adapt results to real
conditions as far as possible. In this article those methods which take
into account the relationship between the deterioration rate of
perishable goods and transportation by ship will be applied with the
aim of (a) minimizing the total cost function, defined as the sum of
the ordering cost, holding cost, disposing cost and transportation
costs, and (b) improving the supply chain sustainability by reducing
environmental impact and waste disposal costs.
Abstract: A feeding experiment was conducted to determine the
optimum dietary protein and lipid levels for juvenile fancy carp. Eight
experimental diets were formulated to contain four protein levels (200,
300, 400 and 500 g kg-1) with two lipid levels (70 and 140 g kg-1).
Triplicate groups of fish (initial weight, 12.1±0.2 g fish-1) were
hand-fed the diets to apparent satiation for 8 weeks. Fish growth
performance, feed utilization and feed intake were significantly
(P0.05). Weight gain and feed efficiency ratio tended to
increase as dietary protein level increased up to 400 and 500 g kg-1,
respectively. Daily feed intake of fish decreased with increasing
dietary protein level and that of fish fed diet contained 500 g kg-1
protein was significantly lower than other fish groups. The protein
efficiency ratio of fish fed 400 and 500 g kg-1 protein was lower than
that of fish fed 200 and 300 g kg-1 protein. Moisture, crude protein and
crude lipid contents of muscle and liver were significantly affected by
dietary protein, but not by dietary lipid level (P>0.05). The increase in
dietary lipid level resulted in an increase in linoleic acid in liver and
muscle paralleled with a decrease in n-3 highly unsaturated fatty acids
content in muscle of fish. In considering these results, it was concluded
that the diet containing 400 g kg-1 protein with 70 g kg-1 lipid level is
optimal for growth and efficient feed utilization of juvenile fancy carp.
Abstract: Due to water shortage, application of saline water for
irrigation is an urgent in agriculture. In this study the effect of
calcium and potassium application as additive in saline root media for
reduce salinity adverse effects was investigated on tomato growth in
a hydroponic system with unequal distribution of salts in the root
media, which was divided in to two equal parts containing full
Johnson nutrient solution and 40 mMNaCl solution, alone or in
combination with KCl (6 mM), CaCl2 (4 mM), K+Ca (3+2 mM) or
half-strength Johnson nutrient solution. The root splits were
exchanged every 7 days. Results showed that addition of calcium,
calcium-potassium and nutrition elements equivalent to half the
concentration of Johnson formula to the saline-half of culture media
minimized the reduction in plant growth caused by NaCl, although
addition of potassium to culture media wasn’t effective. The greatest
concentration of sodium was observed at the shoot of treatments
which had smallest growth. According to the results of this study, in
case of dynamic and non-uniform distribution of salts in the root
media, by addition of additive to the saline solution, it would be
possible to use of saline water with no significant growth reduction.
Abstract: In this paper, effect of marginal quality groundwater
on yield of cotton crop and soil salinity was studied. In this
connection, three irrigation treatments each with four replications
were applied. These treatments were i) use of canal water (T1), ii) use
of marginal quality groundwater from tubewell (T2), and iii)
conjunctive use by mixing with the ratio of 1:1 of canal water and
marginal quality tubewell water (T3).
Water was applied to the crop cultivated in Kharif season 2011; its
quantity has been measured using cut-throat flume. Total 11 watering
each of 50 mm depth have been applied from 20th April to 20th July,
2011. Further, irrigations were stopped due to monsoon rainfall up to
crop harvesting.
Maximum crop yield (seed cotton) was observed under T1 which
was 1,517 kg/ha followed by T3 (mixed canal and tubewell water)
having 1009 kg/ha and T2 i.e. marginal quality groundwater having
709 kg/ha. This concludes that crop yield in T2 and T3 in comparison
to T1was reduced by about 53 and 30% respectively.
It has been observed that yield of cotton crop is below potential
limit for three treatments due to unexpected rainfall at the time of full
flowering season; thus the yield was adversely affected.
However, salt deposition in soil profiles was not observed that is
due to leaching effect of heavy rainfall occurred during monsoon
season.
Abstract: In this study which has been conducted in Akçasu
Forest Range District of Devrek Forest Directorate; 3 methods (weed
control with labourer power, cover removal with Hitachi F20
Excavator, and weed control with agricultural equipment mounted on
a Ferguson 240S agriculture tractor) were utilized in weed control
efforts in regeneration of degraded oriental beech forests have been
compared. In this respect, 3 methods have been compared by
determining certain work hours and standard durations of unit areas
(1 hectare). For this purpose, evaluating the tasks made with human
and machine force from the aspects of duration, productivity and
costs, it has been aimed to determine the most productive method in
accordance with the actual ecological conditions of research field.
Within the scope of the study, the time studies have been conducted
for 3 methods used in weed control efforts. While carrying out those
studies, the performed implementations have been evaluated by
dividing them into business stages. Also, the actual data have been
used while calculating the cost accounts. In those calculations, the
latest formulas and equations which are also used in developed
countries have been utilized. The variance of analysis (ANOVA) was
used in order to determine whether there is any statistically
significant difference among obtained results, and the Duncan test
was used for grouping if there is significant difference. According to
the measurements and findings carried out within the scope of this
study, it has been found during living cover removal efforts in
regeneration efforts in demolished oriental beech forests that the
removal of weed layer in 1 hectare of field has taken 920 hours with
labourer force, 15.1 hours with excavator and 60 hours with an
equipment mounted on a tractor. On the other hand, it has been
determined that the cost of removal of living cover in unit area (1
hectare) was 3220.00 TL for labourer power, 1250 TL for excavator
and 1825 TL for equipment mounted on a tractor.
According to the obtained results, it has been found that the
utilization of excavator in weed control effort in regeneration of
degraded oriental beech regions under actual ecological conditions of
research field has been found to be more productive from both of
aspects of duration and costs. These determinations carried out
should be repeated in weed control efforts in degraded forest fields
with different ecological conditions, it is compulsory for finding the
most efficient weed control method. These findings will light the way
of technical staff of forestry directorate in determination of the most
effective and economic weed control method. Thus, the more actual
data will be used while preparing the weed control budgets, and there
will be significant contributions to national economy. Also the results of this and similar studies are very important for developing the policies for our forestry in short and long term.
Abstract: The tomato is a very important crop, whose
cultivation in the Mediterranean basin is severely affected by the
phytoparasitic weed Phelipanche ramosa. The semiarid regions of
the world are considered the main areas where this parasitic weed is
established causing heavy infestation as it is able to produce high
numbers of seeds (up to 500,000 per plant), which remain viable for
extended period (more than 20 years). In this paper the results
obtained from eleven treatments in order to control this parasitic
weed including chemical, agronomic, biological and biotechnological
methods compared with the untreated test under two plowing depths
(30 and 50 cm) are reported. The split-plot design with 3 replicates
was adopted. In 2014 a trial was performed in Foggia province
(southern Italy) on processing tomato (cv Docet) grown in the field
infested by Phelipanche ramosa. Tomato seedlings were transplant
on May 5, on a clay-loam soil. During the growing cycle of the
tomato crop, at 56-78 and 92 days after transplantation, the number
of parasitic shoots emerged in each plot was detected. At tomato
harvesting, on August 18, the major quantity-quality yield parameters
were determined (marketable yield, mean weight, dry matter, pH,
soluble solids and color of fruits). All data were subjected to analysis
of variance (ANOVA) and the means were compared by Tukey's test.
Each treatment studied did not provide complete control against
Phelipanche ramosa. However, among the different methods tested,
some of them which Fusarium, gliphosate, radicon biostimulant and
Red Setter tomato cv (improved genotypes obtained by Tilling
technology) under deeper plowing (50 cm depth) proved to mitigate
the virulence of the Phelipanche ramose attacks. It is assumed that
these effects can be improved combining some of these treatments
each other, especially for a gradual and continuing reduction of the
“seed bank” of the parasite in the soil.
Abstract: Heavy metals are one of the major groups of
contaminants in the environment and many of them are toxic even at
very low concentration in plants and animals. However, some metals
play important roles in the biological function of many enzymes in
living organisms. Metals such as zinc, iron, and cooper are important
for survival and activity of enzymes in plants, however heavy metals
can inhibit enzyme which is responsible for defense system of plants.
Polyphenol oxidase (PPO) is a copper-containing metalloenzyme
which is responsible for enzymatic browning reaction of plants.
Enzymatic browning is a major problem for the handling of
vegetables and fruits in food industry. It can be increased and
effected with many different futures such as metals in the nature and
ground. In the present work, PPO was isolated and characterized
from green leaves of red poppy plant (Papaverr hoeas). Then, the
effect of some known antibrowning agents which can form
complexes with metals and metals were investigated on the red poppy
PPO activity. The results showed that glutathione was the most
potent inhibitory effect on PPO activity. Cu(II) and Fe(II) metals
increased the enzyme activities however, Sn(II) had the maximum
inhibitory effect and Zn(II) and Pb(II) had no significant effect on the
enzyme activity. In order to reduce the effect of heavy metals, the
effects of metal-antibrowning agent complexes on the PPO activity
were determined. EDTA and metal complexes had no significant
effect on the enzyme. L-ascorbic acid and metal complexes decreased
but L-ascorbic acid-Cu(II)-complex had no effect. Glutathione–metal
complexes had the best inhibitory effect on Red poppy leaf PPO
activity.
Abstract: The small interfering RNA (siRNA) alters the
regulatory role of mRNA during gene expression by translational
inhibition. Recent studies show that upregulation of mRNA because
serious diseases like cancer. So designing effective siRNA with good
knockdown effects plays an important role in gene silencing. Various
siRNA design tools had been developed earlier. In this work, we are
trying to analyze the existing good scoring second generation siRNA
predicting tools and to optimize the efficiency of siRNA prediction
by designing a computational model using Artificial Neural Network
and whole stacking energy (%G), which may help in gene silencing
and drug design in cancer therapy. Our model is trained and tested
against a large data set of siRNA sequences. Validation of our results
is done by finding correlation coefficient of experimental versus
observed inhibition efficacy of siRNA. We achieved a correlation
coefficient of 0.727 in our previous computational model and we
could improve the correlation coefficient up to 0.753 when the
threshold of whole tacking energy is greater than or equal to -32.5
kcal/mol.