Selection of Saccharomyces cerevisiae Strains Tolerant to Lead and Cadmium Toxicity
The aim of this study was to select the best strains of
Saccharomyces cerevisiae able to resist lead and cadmium. Ten
strains were screened on the basis of their resistance at different
concentrations of 0, 2, 4, 8, 12, 16, 20 and 24 ppm for Pb and 0, 0.5,
1, 2, 4, 6, 8 and 10 ppm for Cd. The properties of baker's yeast
quality were decreased by the increase of Pb or Cd in growth
medium. The slope values of yield, total viable cells and gassing
power of produced baker's yeast were investigated as an indicator of
metal resistant. In addition, concentrations of Pb and Cd in produced
baker's yeast were determined. The strain of S. cerevisiae FH-620
had the highest resistance against Pb and Cd and had the minimum
levels of both two investigated metals in produced baker's yeast.
[1] W. Damtew. Studies on the development of baker’s yeast using cane
molasses. M.Sc. Thesis, Fac. Technol., Addis Ababa Univ., Addis
Ababa, 2008, 189 p.
[2] P. Iatskovskaia, G. I. Solomko, I. V. Kononko and V. K. Ianchevskii.
Chemical composition of protein concentrate from Saccharomyces and
its effect on immunologic response. Vopr pitan., Jan-Feb., 1992 (1) pp.
63-67.
[3] L. V. Curtin. Molasses-General Considerations. In: Molasses in Animal
Nutrition (Ed. Curtin, L. V.), National Feed Ingredients Association,
West Des Moines, USA, 1983, pp. 211-235.
[4] H. Togrul and N. Arslan. Mathematical model for prediction of apparent
viscosity of molasses. Journal of Food Engineering, 2004, vol. 62 pp.
281–289.
[5] G. N. Abdel-Rahman. Effect of some heavy metals in molasses medium
on the produced baker's yeast properties. M.Sc. Thesis, Food
Technology Dep. Fac. Agric., Cairo Univ., Egypt, 2010, 171 p.
[6] I. S. Ross. Some effects of heavy metals on fungal cells. Trans. Br.
Mycol. Soc., 1975, vol. 64 pp. 175-193.
[7] G. M. Gadd. Interactions of fungi with toxic metals. New Phytol., 1993,
vol. 124 pp. 25-60.
[8] E. V. Soares, K. Hebbelinck and H. M. V. M. Soares. Toxic effects
caused by heavy metals in the yeast Saccharomyces cerevisiae: a
comparative study. Can J. Microbiol., 2003, vol. 49 pp. 336-343.
[9] P. Skountzou, M. Soupioni, A. Bekatorou, M. Kanellaki, A. A.
Koutinas, R. Marchant and I. M. Banat. Lead (II) uptake during baker’s
yeast production by aerobic fermentation of molasses. Process
Biochemistry, 2003, vol. 38 pp. 1479-1482.
[10] V. Brandolini, P. Tedeschi, A. Capece, A. Maietti, D. Mazzotta, G.
Salzano, A. Paparella and P. Romano. Saccharomyces cerevisiae wine
strains differing in copper resistance exhibit different capability to
reduce copper content in wine. World Journal of Microbiology &
Biotechnology, 2002, vol. 18 pp. 499–503.
[11] C. Li, Y. Xu, W. Jiang, X. Dong, D. Wang and B. Liu. Effect of NaCl on
the heavy metal tolerance and bioaccumulation of Zygosaccharomyces
rouxii and Saccharomyces cerevisiae. Bioresource Technology, 2013,
vol. 143 pp. 46–52.
[12] Egyptian Standard. Egyptian Standard of yeast Part 2: methods of
analysis and testing for yeast. Egyptian Organization for Standardization
and Quality Control, E.S. 191/2000.
[13] M. L. Suihko and V. Mfikinen. Candida krusei in baker's yeast
production. European J Appl Microbiol Biotechnol., 1981, vol. 13 pp.
113-116.
[14] SAS: Statistical Analysis System, SAS / STAT User's Guide. Release
6.03 Edn. SAS Institute, Cary, NC, 1028 PP., 1999.
[15] P. D. B. Adamis, D. S. Gomes, M. L. C. C. Pinto, A. D. Panek and E. C.
A. Eleutherio. The role of glutathione transferases in cadmium stress.
Toxicology Letters, 2004, vol. 154 pp. 81–88.
[16] M. Mapolelo, N. Torto and B. Prior. Evaluation of yeast strains as
possible agents for trace enrichment of metal ions in aquatic
environments. Talanta, 2005, vol. 65 pp. 930–937.
[17] D. S. Gomes, L. C. Fragoso, C. J. Riger, A. D. Panek and E. C. A.
Eleutherio. Regulation of cadmium uptake by Saccharomyces
cerevisiae. Biochimica et Biophysica Acta, 2002, vol. 1573 pp. 21- 25.
[18] M. Schmitt, G. Gellert, J. Ludwig and H. L. Frate. Phenotypic yeast
growth analysis for chronic toxicity testing. Ecotoxicology and
Environmental Safety, 2004, vol. 59 pp. 142–150.
[19] S. Vinopal, T. Ruml and P. Kotrba. Biosorption of Cd2+ and Zn2+ by cell
surface-engineered Saccharomyces cerevisiae. International
Biodeterioration and Biodegradation, 2007, vol. 60 pp. 96-102.
[20] F. Ghorbani, H. Younesi, S. M. Ghasempouri, A. A. Zinatizadeh, M.
Amini and A. Daneshi. Application of response surface methodology for
optimization of cadmium biosorption in an aqueous solution by
Saccharomyces cerevisiae. Chem. Eng. J., 2008, vol. 145 pp. 267-275.
[21] F. Di Caprio, P. Altimari, D. Uccelletti and F. Pagnanelli. Mechanistic
modelling of copper biosorption by wild type and engineered
Saccharomyces cerevisiae biomasses. Chemical Engineering Journal,
2014, vol. 244 pp. 561-568.
[22] W. Jiang, Y. Xu, C. Li, X. Lv and D. Wang. Effect of inorganic salts on
the growth and Cd2+ bioaccumulation of Zygosaccharomyces rouxii
cultured under Cd2+ stress. Bioresour. Technol., 2013, vol. 128 pp. 831-
834.
[23] I. Lefevre, G. Marchal, P. Meerts, E. Correal and S. Lutts. Chloride
salinity reduces cadmium accumulation by the Mediterranean halophyte
species Atriplex halimus L. Environ. Exp. Bot, 2009, vol. 65 pp. 142-
152.
[24] S. A. H. Munoz, K. Wrobel, G. J. F. Corona and K. Wrobel. The
protective effect of selenium inorganic forms against cadmium and
silver toxicity in mycelia of Pleurotus ostreatus. Mycol. Res., 2007, vol.
111 pp. 626-632.
[25] M. Strouhal, R. Kizek, J. Vacek, L. Trnkova and M. Nemec.
Electrochemical study of heavy metals and metallothionein in yeast
Yarrowia lipolytica. Bioelectrochemistry, 2003, vol. 60 pp. 29-36.
[1] W. Damtew. Studies on the development of baker’s yeast using cane
molasses. M.Sc. Thesis, Fac. Technol., Addis Ababa Univ., Addis
Ababa, 2008, 189 p.
[2] P. Iatskovskaia, G. I. Solomko, I. V. Kononko and V. K. Ianchevskii.
Chemical composition of protein concentrate from Saccharomyces and
its effect on immunologic response. Vopr pitan., Jan-Feb., 1992 (1) pp.
63-67.
[3] L. V. Curtin. Molasses-General Considerations. In: Molasses in Animal
Nutrition (Ed. Curtin, L. V.), National Feed Ingredients Association,
West Des Moines, USA, 1983, pp. 211-235.
[4] H. Togrul and N. Arslan. Mathematical model for prediction of apparent
viscosity of molasses. Journal of Food Engineering, 2004, vol. 62 pp.
281–289.
[5] G. N. Abdel-Rahman. Effect of some heavy metals in molasses medium
on the produced baker's yeast properties. M.Sc. Thesis, Food
Technology Dep. Fac. Agric., Cairo Univ., Egypt, 2010, 171 p.
[6] I. S. Ross. Some effects of heavy metals on fungal cells. Trans. Br.
Mycol. Soc., 1975, vol. 64 pp. 175-193.
[7] G. M. Gadd. Interactions of fungi with toxic metals. New Phytol., 1993,
vol. 124 pp. 25-60.
[8] E. V. Soares, K. Hebbelinck and H. M. V. M. Soares. Toxic effects
caused by heavy metals in the yeast Saccharomyces cerevisiae: a
comparative study. Can J. Microbiol., 2003, vol. 49 pp. 336-343.
[9] P. Skountzou, M. Soupioni, A. Bekatorou, M. Kanellaki, A. A.
Koutinas, R. Marchant and I. M. Banat. Lead (II) uptake during baker’s
yeast production by aerobic fermentation of molasses. Process
Biochemistry, 2003, vol. 38 pp. 1479-1482.
[10] V. Brandolini, P. Tedeschi, A. Capece, A. Maietti, D. Mazzotta, G.
Salzano, A. Paparella and P. Romano. Saccharomyces cerevisiae wine
strains differing in copper resistance exhibit different capability to
reduce copper content in wine. World Journal of Microbiology &
Biotechnology, 2002, vol. 18 pp. 499–503.
[11] C. Li, Y. Xu, W. Jiang, X. Dong, D. Wang and B. Liu. Effect of NaCl on
the heavy metal tolerance and bioaccumulation of Zygosaccharomyces
rouxii and Saccharomyces cerevisiae. Bioresource Technology, 2013,
vol. 143 pp. 46–52.
[12] Egyptian Standard. Egyptian Standard of yeast Part 2: methods of
analysis and testing for yeast. Egyptian Organization for Standardization
and Quality Control, E.S. 191/2000.
[13] M. L. Suihko and V. Mfikinen. Candida krusei in baker's yeast
production. European J Appl Microbiol Biotechnol., 1981, vol. 13 pp.
113-116.
[14] SAS: Statistical Analysis System, SAS / STAT User's Guide. Release
6.03 Edn. SAS Institute, Cary, NC, 1028 PP., 1999.
[15] P. D. B. Adamis, D. S. Gomes, M. L. C. C. Pinto, A. D. Panek and E. C.
A. Eleutherio. The role of glutathione transferases in cadmium stress.
Toxicology Letters, 2004, vol. 154 pp. 81–88.
[16] M. Mapolelo, N. Torto and B. Prior. Evaluation of yeast strains as
possible agents for trace enrichment of metal ions in aquatic
environments. Talanta, 2005, vol. 65 pp. 930–937.
[17] D. S. Gomes, L. C. Fragoso, C. J. Riger, A. D. Panek and E. C. A.
Eleutherio. Regulation of cadmium uptake by Saccharomyces
cerevisiae. Biochimica et Biophysica Acta, 2002, vol. 1573 pp. 21- 25.
[18] M. Schmitt, G. Gellert, J. Ludwig and H. L. Frate. Phenotypic yeast
growth analysis for chronic toxicity testing. Ecotoxicology and
Environmental Safety, 2004, vol. 59 pp. 142–150.
[19] S. Vinopal, T. Ruml and P. Kotrba. Biosorption of Cd2+ and Zn2+ by cell
surface-engineered Saccharomyces cerevisiae. International
Biodeterioration and Biodegradation, 2007, vol. 60 pp. 96-102.
[20] F. Ghorbani, H. Younesi, S. M. Ghasempouri, A. A. Zinatizadeh, M.
Amini and A. Daneshi. Application of response surface methodology for
optimization of cadmium biosorption in an aqueous solution by
Saccharomyces cerevisiae. Chem. Eng. J., 2008, vol. 145 pp. 267-275.
[21] F. Di Caprio, P. Altimari, D. Uccelletti and F. Pagnanelli. Mechanistic
modelling of copper biosorption by wild type and engineered
Saccharomyces cerevisiae biomasses. Chemical Engineering Journal,
2014, vol. 244 pp. 561-568.
[22] W. Jiang, Y. Xu, C. Li, X. Lv and D. Wang. Effect of inorganic salts on
the growth and Cd2+ bioaccumulation of Zygosaccharomyces rouxii
cultured under Cd2+ stress. Bioresour. Technol., 2013, vol. 128 pp. 831-
834.
[23] I. Lefevre, G. Marchal, P. Meerts, E. Correal and S. Lutts. Chloride
salinity reduces cadmium accumulation by the Mediterranean halophyte
species Atriplex halimus L. Environ. Exp. Bot, 2009, vol. 65 pp. 142-
152.
[24] S. A. H. Munoz, K. Wrobel, G. J. F. Corona and K. Wrobel. The
protective effect of selenium inorganic forms against cadmium and
silver toxicity in mycelia of Pleurotus ostreatus. Mycol. Res., 2007, vol.
111 pp. 626-632.
[25] M. Strouhal, R. Kizek, J. Vacek, L. Trnkova and M. Nemec.
Electrochemical study of heavy metals and metallothionein in yeast
Yarrowia lipolytica. Bioelectrochemistry, 2003, vol. 60 pp. 29-36.
@article{"International Journal of Biological, Life and Agricultural Sciences:69240", author = "Nadia R. A. Nassar and Yehia A. Heikal and Mahmoud A. M. Abou Donia and Mohamed Fadel and Gomaa N. Abdel-Rahman", title = "Selection of Saccharomyces cerevisiae Strains Tolerant to Lead and Cadmium Toxicity", abstract = "The aim of this study was to select the best strains of
Saccharomyces cerevisiae able to resist lead and cadmium. Ten
strains were screened on the basis of their resistance at different
concentrations of 0, 2, 4, 8, 12, 16, 20 and 24 ppm for Pb and 0, 0.5,
1, 2, 4, 6, 8 and 10 ppm for Cd. The properties of baker's yeast
quality were decreased by the increase of Pb or Cd in growth
medium. The slope values of yield, total viable cells and gassing
power of produced baker's yeast were investigated as an indicator of
metal resistant. In addition, concentrations of Pb and Cd in produced
baker's yeast were determined. The strain of S. cerevisiae FH-620
had the highest resistance against Pb and Cd and had the minimum
levels of both two investigated metals in produced baker's yeast.
", keywords = "Cadmium, lead, S. cerevisiae, tolerant.", volume = "9", number = "1", pages = "93-8", }
