Abstract: Anammox is a novel and promising technology that has changed the traditional concept of biological nitrogen removal. The process facilitates direct oxidation of ammonical nitrogen under anaerobic conditions with nitrite as an electron acceptor without addition of external carbon sources. The present study investigated the feasibility of Anammox Hybrid Reactor (AHR) combining the dual advantages of suspended and attached growth media for biodegradation of ammonical nitrogen in wastewater. Experimental unit consisted of 4 nos. of 5L capacity AHR inoculated with mixed seed culture containing anoxic and activated sludge (1:1). The process was established by feeding the reactors with synthetic wastewater containing NH4-H and NO2-N in the ratio 1:1 at HRT (hydraulic retention time) of 1 day. The reactors were gradually acclimated to higher ammonium concentration till it attained pseudo steady state removal at a total nitrogen concentration of 1200 mg/l. During this period, the performance of the AHR was monitored at twelve different HRTs varying from 0.25-3.0 d with increasing NLR from 0.4 to 4.8 kg N/m3d. AHR demonstrated significantly higher nitrogen removal (95.1%) at optimal HRT of 1 day. Filter media in AHR contributed an additional 27.2% ammonium removal in addition to 72% reduction in the sludge washout rate. This may be attributed to the functional mechanism of filter media which acts as a mechanical sieve and reduces the sludge washout rate many folds. This enhances the biomass retention capacity of the reactor by 25%, which is the key parameter for successful operation of high rate bioreactors. The effluent nitrate concentration, which is one of the bottlenecks of anammox process was also minimised significantly (42.3-52.3 mg/L). Process kinetics was evaluated using first order and Grau-second order models. The first-order substrate removal rate constant was found as 13.0 d-1. Model validation revealed that Grau second order model was more precise and predicted effluent nitrogen concentration with least error (1.84±10%). A new mathematical model based on mass balance was developed to predict N2 gas in AHR. The mass balance model derived from total nitrogen dictated significantly higher correlation (R2=0.986) and predicted N2 gas with least error of precision (0.12±8.49%). SEM study of biomass indicated the presence of heterogeneous population of cocci and rod shaped bacteria of average diameter varying from 1.2-1.5 mm. Owing to enhanced NRE coupled with meagre production of effluent nitrate and its ability to retain high biomass, AHR proved to be the most competitive reactor configuration for dealing with nitrogen laden wastewater.
Abstract: Rice straw is lignocellulosic biomass which can be utilized as substrate for the biogas production. However, due to the property and composition of rice straw, it is difficult to be degraded by hydrolysis enzymes. One of the pretreatment methods that modify such properties of lignocellulosic biomass is the application of lignocellulose-degrading microbial consortia. The aim of this study is to investigate the effect of microbial consortia to enhance biogas production. To select the high efficient consortium, cellulase enzymes were extracted and their activities were analyzed. The results suggested that microbial consortium culture obtained from cattle manure is the best candidate compared to decomposed wood and horse manure. A microbial consortium isolated from cattle manure was then mixed with anaerobic sludge and used as inoculum for biogas production. The optimal conditions for biogas production were investigated using response surface methodology (RSM). The tested parameters were the ratio of amount of microbial consortium isolated and amount of anaerobic sludge (MI:AS), substrate to inoculum ratio (S:I) and temperature. Here, the value of the regression coefficient R2 = 0.7661 could be explained by the model which is high to advocate the significance of the model. The highest cumulative biogas yield was 104.6 ml/g-rice straw at optimum ratio of MI:AS, ratio of S:I, and temperature of 2.5:1, 15:1 and 44°C respectively.
Abstract: With demand for primary energy continuously
growing, search for renewable and efficient energy sources has been
high on agenda of our society. One of the most promising energy
sources is biogas technology. Residues coming from dairy industry
and milk processing could be used in biogas production; however,
low efficiency and high cost impede wide application of such
technology. One of the main problems is management and conversion
of organic residues through the anaerobic digestion process which is
characterized by acidic environment due to the low whey pH (
Abstract: Implementing of a continues flow gas lift system for one vertical oil well producer in Wafa field was investigated under five reservoir pressures and their dependent parameters. Well 03 producers were responded positively to the gas lift system despite of the high well head operating pressures. However, the flowing bottom hole pressures were reduced by a ratio from 6 to 33 % in the case A3 for example, for the design runs conducted under the existing operating conditions for years 2003, 2006 and 2009. This reduction in FBHP has increased the production rate by a ratio from 12 to 22.5%. The results indicated that continues flow gas lift system is a good candidate as an artificial lift system to be considered for the one vertical producer covered by this study. Most significantly, timing for artificial lift by a gas lift system for this field is highly dependent on the amount of gas available at the time of implementation because of the high gas production rate from the top of the reservoir.
Abstract: The aim of this study was to obtain the optimal conditions for biogas production from anaerobic digestion of organic fraction of municipal solid waste (OFMSW) using response surface methodology (RSM). The parameters studied were initial pH, substrate concentration and total organic carbon (TOC). The experimental results showed that the linear model terms of initial pH and substrate concentration and the quadratic model terms of the substrate concentration and TOC had significant individual effect (p < 0.05) on biogas yield. However, there was no interactive effect between these variables (p > 0.05). The highest level of biogas produced was 53.4 L/Kg VS at optimum pH, substrate concentration and total organic carbon of 6.5, 99gTS/L and 20.32 g/L respectively.
Abstract: The effect of carbon materials on TiO2 for the photocatalytic hydrogen gas production from water / alcohol mixtures was investigated. Single walled carbon nanotubes (SWNTs), multi walled carbon nanotubes (MWNTs), carbon nanofiber (CNF), fullerene (FLN), graphite (GP), and graphite silica (GS) were used as co-catalysts by directly mixing with TiO2. Drastic synergy effects were found with increase in the amount of hydrogen gas by a factor of ca. 150 and 100 for SWNTs and GS with TiO2, respectively. Moreover, the increment factor of hydrogen production reached to 180, when the mixture of SWNTs and TiO2 were smashed in an agate mortar before photocatalytic reactions. The order of H2 gas production for these carbon materials was SWNTs > GS >> MWNTs > FLN > CNF > GP. To maximize the hydrogen production from SWNTs/TiO2, various parameters of experimental condition were changed. Also, a comparison between Pt/TiO2, SWNTs/TiO2 and GS/TiO2 was made for the amount of H2 gas production. Finally, the recyclability of SWNTs/TiO2or GS/TiO2 was tested.
Abstract: The present study was undertaken with the objective of evaluating kitchen waste as an alternative organic material for biogas production in community level biogas plant. The field study was carried out for one month (January 19, 2012– February 17, 2012) at Centre for Energy Studies, IIT Delhi, New Delhi, India.
This study involves the uses of greenhouse canopy to increase the temperature for the production of biogas in winter period. In continuation, a semi-continuous study was conducted for one month with the retention time of 30 days under batch system. The gas generated from the biogas plant was utilized for cooking (burner) and lighting (lamp) purposes. Gas productions in the winter season registered lower than other months. It can be concluded that the solar greenhouse assisted biogas plant can be efficiently adopted in colder region or in winter season because temperature plays a major role in biogas production.
Abstract: Anaerobic digestion process is one of the alternative
methods to convert organic waste into methane gas which is a fuel
and energy source. Activities of various kinds of microorganisms are
the main factor for anaerobic digestion which produces methane gas.
Therefore, in this study a modified Anaerobic Baffled Reactor (ABR)
with working volume of 50 liters was designed to identify the
microorganisms through biogas production. The mixture of 75%
kitchen waste and 25% sewage sludge was used as substrate.
Observations on microorganisms in the ABR showed that there exists
a small amount of protozoa (5%) and fungi (2%) in the system, but
almost 93% of the microorganism population consists of bacteria. It
is definitely clear that bacteria are responsible for anaerobic
biodegradation of kitchen waste. Results show that in the
acidification zone of the ABR (front compartments of reactor) fast
growing bacteria capable of growth at high substrate levels and
reduced pH was dominant. A shift to slower growing scavenging
bacteria that grow better at higher pH was occurring towards the end
of the reactor. Due to the ability of activity in acetate environment the
percentages of Methanococcus, Methanosarcina and Methanotrix
were higher than other kinds of methane former in the system.
Abstract: Production of biogas from bakery waste was enhanced
by additional bacterial cell. This study was divided into 2 steps. First
step, grease waste from bakery industry-s grease trap was initially
degraded by Pseudomonas aeruginosa. The concentration of byproduct,
especially glycerol, was determined and found that glycerol
concentration increased from 12.83% to 48.10%. Secondary step, 3
biodigesters were set up in 3 different substrates: non-degraded waste
as substrate in first biodigester, degraded waste as substrate in
secondary biodigester, and degraded waste mixed with swine manure
in ratio 1:1 as substrate in third biodigester. The highest
concentration of biogas was found in third biodigester that was
44.33% of methane and 63.71% of carbon dioxide. The lower
concentration at 24.90% of methane and 18.98% of carbon dioxide
was exhibited in secondary biodigester whereas the lowest was found
in non-degraded waste biodigester. It was demonstrated that the
biogas production was greatly increased with the initial grease waste
degradation by Pseudomonas aeruginosa.
Abstract: This paper aims to study decomposition behavior in
pyrolytic environment of four lignocellulosic biomass (oil palm shell,
oil palm frond, rice husk and paddy straw), and two commercial
components of biomass (pure cellulose and lignin), performed in a
thermogravimetry analyzer (TGA). The unit which consists of a
microbalance and a furnace flowed with 100 cc (STP) min-1 Nitrogen,
N2 as inert. Heating rate was set at 20⁰C min-1 and temperature
started from 50 to 900⁰C. Hydrogen gas production during the
pyrolysis was observed using Agilent Gas Chromatography Analyzer
7890A. Oil palm shell, oil palm frond, paddy straw and rice husk
were found to be reactive enough in a pyrolytic environment of up to
900°C since pyrolysis of these biomass starts at temperature as low as
200°C and maximum value of weight loss is achieved at about
500°C. Since there was not much different in the cellulose,
hemicelluloses and lignin fractions between oil palm shell, oil palm
frond, paddy straw and rice husk, the T-50 and R-50 values obtained
are almost similar. H2 productions started rapidly at this temperature
as well due to the decompositions of biomass inside the TGA.
Biomass with more lignin content such as oil palm shell was found to
have longer duration of H2 production compared to materials of high
cellulose and hemicelluloses contents.
Abstract: Factor analysis was applied to two stages biogas
production from banana stem waste allowing a screening of the
experimental variables second stage temperature (T), organic loading
rates (OLR) and hydraulic retention times (HRT). Biogas production
was found to be strongly influenced by all the above experimental
variables. Results from factorial analysis have shown that all
variables which were HRT, OLR and T have significant effect to
biogas production. Increased in HRT and OLR could increased the
biogas yield. The performance was tested under the conditions of
various T (35oC-60oC), OLR (0.3 g TS/l.d–1.9 gTS/l.d), and HRT (3
d–15 d). Conditions for temperature, OLR and HRT in this study
were based on the best range obtained from literature review.
Abstract: The present investigation was undertaken to explore the biogas potentiality of Jatropha (Jatropha curcas, Euphorbiaceae) Fruit Coat (JFC) alone and in combination with cattle dung (CD) in various proportions at 15 per cent total solids by batch phase anaerobic digestion for a period of ten weeks HRT (Hydraulic Retention Time) under a temperature of 35°C+1°C. The maximum biogas production was noticed in Cattle dung and Jatropha Fruit Coat in 2:1 ratio with 403.84 L/kg dry matter followed by 3:1,1:2, 1:1 and 1:3 having 329.66, 219.77, 217.79, 203.64 L /kg dm respectively as compared to 178.49 L/kg dm in CD alone. The JFC alone found to produce 91 per cent of total biogas that obtained from Cattle dung. The per cent methane content of the biogas in all the treatments was found on par with Cattle dung.
Abstract: Municipal solid waste (MSW) comprises of a wide
range of heterogeneous materials generated by individual, household
or organization and may include food waste, garden wastes, papers,
textiles, rubbers, plastics, glass, ceramics, metals, wood wastes,
construction wastes but it is not limited to the above mentioned
fractions. The most common Municipal Solid Waste pretreatment
method in use is thermal pretreatment (incineration) and Mechanical
Biological pretreatment. This paper presents an overview of these
two pretreatment methods describing their benefits and laboratory
scale reactors that simulate landfill conditions were constructed in
order to compare emissions in terms of biogas production and
leachate contamination between untreated Municipal Solid Waste and
Mechanical Biological Pretreated waste. The findings of this study
showed that Mechanical Biological pretreatment of waste reduces the
emission level of waste and the benefit over the landfilling of
untreated waste is significant.
Abstract: In this study, the kinetic of biogas production was studied by performing a series laboratory experiment using rumen fluid of animal ruminant as inoculums. Cattle manure as substrate was inoculated by rumen fluid to the anaerobic biodigester. Laboratory experiments using 400 ml biodigester were performed in batch operation mode. Given 100 grams of fresh cattle manure was fed to each biodigester and mixed with rumen fluid by manure : rumen weight ratio of 1:1 (MR11). The operating temperatures were varied at room temperature and 38.5 oC. The cumulative volume of biogas produced was used to measure the biodigester performance. The research showed that the rumen fluid inoculated to biodigester gave significant effect to biogas production (P
Abstract: Landfill gas, particularly methane is one of the
greenhouse gases which contributes to global warming. This paper presents the findings of a study on methane gas production from
simulated landfill reactor under saturated conditions. A reactor was constructed to represent a landfill cell of 2.5 m thickness on sandy
soil. The reactor was 0.2 m in diameter and 4 m in height. One meter of sand and pebble layer was packed at the bottom of the reactor
followed by 2.5 m of solid waste layer and 0.4 m of sand layer as the cover soil. Degradation of waste in the solid waste layer was at
acidification stage as indicated by the leachate quality with COD as
high as 55,511 mg/L and pH as low as 5.1. However, methanogenic
environment was established at the bottom sand layer after one year of operation indicated by pH of 7.2 and methane gas generation.
Leachate degradation took place as the leachate moved through the
sand layer at an infiltration of rate 0.7 cm/day. This resulted in landfill gas production of 77 mL/day/kg containing 55 to 65% methane. The application of sand layer contributed to the gas
production from landfill by an in-situ degradation of leachate in the
sand at the bottom of the landfill.
Abstract: In this research, an anaerobic co-digestion using decanter cake from palm oil mill industry to improve the biogas production from frozen seafood wastewater is studied using Continuously Stirred Tank Reactor (CSTR) process. The experiments were conducted in laboratory-scale. The suitable Hydraulic Retention Time (HRT) was observed in CSTR experiments with 24 hours of mixing time using the mechanical mixer. The HRT of CSTR process impacts on the efficiency of biogas production. The best performance for biogas production using CSTR process was the anaerobic codigestion for 20 days of HRT with the maximum methane production rate of 1.86 l/d and the average maximum methane production of 64.6%. The result can be concluded that the decanter cake can improve biogas productivity of frozen seafood wastewater.
Abstract: The use of renewable energy sources incl. biogas has become topical in accordance with the increasing demand for energy, decrease of fossil energy resources and the efforts to reduce greenhouse gas emissions as well as to increase energy independence from the territories where fossil energy resources are available.
As the technologies of biogas production from agricultural biomass develop, risk assessment and risk management become necessary for farms producing such a renewable energy. The need for risk assessments has become particularly topical when discussions on changing the biogas policy in the EU take place, which may influence the development of the sector in the future, as well as the operation of existing biogas facilities and their income level.
The current article describes results of the risk assessment for farms producing biomass from agriculture biomass in Latvia, the risk assessment system included 24 risks, that affect the whole biogas production process and the obtained results showed the high significance of political and production risks.
Abstract: This paper discusses the utilization of marine biomass as an energy resource in Japan. A marine biomass energy system in Japan was proposed consisting of seaweed cultivation (Laminaria japonica) at offshore marine farms, biogas production via methane fermentation of the seaweeds, and fuel cell power generation driven by the generated biogas. We estimated energy output, energy supply potential, and CO2 mitigation in Japan on the basis of the proposed system. As a result, annual energy production was estimated to be 1.02-109 kWh/yr at nine available sites. Total CO2 mitigation was estimated to be 1.04-106 tonnes per annum at the nine sites. However, the CO2 emission for the construction of relevant facilities is not taken into account in this paper. The estimated CO2 mitigation is equivalent to about 0.9% of the required CO2 mitigation for Japan per annum under the Kyoto Protocol framework.
Abstract: A horizontal anaerobic digester was developed and
tested in pilot scale for Korean food waste with high water contents
(>80%). The hydrogen sulfide in the biogas was removed by a
biological desulfurization equipment integrated in the horizontal
digester. A mixer of the horizontal digester was designed to easily
remove the sediment in the bottom and scum layers on surface in the
digester. Experimental result for 120 days of operation of the pilot
plant showed a high removal efficiency of 81.2% for organic
substance and high stability during the whole operation period were
acquired. Also food waste was treated at high organic loading rates
over 4 kg•VS/m3∙day and a methane gas production rate of 0.62
m3/kg•VSremoved was accomplished.
The biological desulfurization equipment inside the horizontal
digester was proven to be an economic and effective method to reduce
the biogas desulfurization cost by removing hydrogen sulfide more
than 90% without external desulfurization equipments.
Abstract: Anaerobic treatment has many advantages over other
biological method particularly when used to treat complex
wastewater such as petroleum refinery wastewater. In this study two
Up-flow Anaerobic Sludge Blanket (UASB) reactors were operated
in parallel to treat six volumetric organic loads (0.58, 1.21, 0.89,
2.34, 1.47 and 4.14 kg COD/m3·d) to evaluate the chemical oxygen
demand (COD) removal efficiency. The reactors were continuously
adapting to the changing of operation condition with increase in the
removal efficiency or slight decrease until the last load which was
more than two times the load, at which the reactor stressed and the
removal efficiency decreased to 75% with effluent concentration of
1746 mg COD/L. Other parameters were also monitored such as pH,
alkalinity, volatile fatty acid and gas production rate. The UASB
reactor was suitable to treat petroleum refinery wastewater and the
highest COD removal rate was 83% at 1215 kg/m3·d with COD
concentration about 356 mg/L in the effluent.