Analysis of a Lignocellulose Degrading Microbial Consortium to Enhance the Anaerobic Digestion of Rice Straws
Rice straw is lignocellulosic biomass which can be utilized as substrate for the biogas production. However, due to the property and composition of rice straw, it is difficult to be degraded by hydrolysis enzymes. One of the pretreatment methods that modify such properties of lignocellulosic biomass is the application of lignocellulose-degrading microbial consortia. The aim of this study is to investigate the effect of microbial consortia to enhance biogas production. To select the high efficient consortium, cellulase enzymes were extracted and their activities were analyzed. The results suggested that microbial consortium culture obtained from cattle manure is the best candidate compared to decomposed wood and horse manure. A microbial consortium isolated from cattle manure was then mixed with anaerobic sludge and used as inoculum for biogas production. The optimal conditions for biogas production were investigated using response surface methodology (RSM). The tested parameters were the ratio of amount of microbial consortium isolated and amount of anaerobic sludge (MI:AS), substrate to inoculum ratio (S:I) and temperature. Here, the value of the regression coefficient R2 = 0.7661 could be explained by the model which is high to advocate the significance of the model. The highest cumulative biogas yield was 104.6 ml/g-rice straw at optimum ratio of MI:AS, ratio of S:I, and temperature of 2.5:1, 15:1 and 44°C respectively.
[1] L. A. Lucia, “Lignocellulosic biomass: Replace petroleum,” Raleigh,
N.C., 2008, pp.981-982.
[2] Data Retrieved from the link: www.irri.org
[3] Data Retrieved from the link: www.dede.go.th
[4] P. S. Nigam, N. Gupta, and A. Anthwal, “Pre-treatment of agroindustrial
residues,” in Biotechnology for agro-industrial residues
utilization, 1st ed. Netherlands: Springer, 2009, pp. 13-33.
[5] S. Wongwilaiwalina, U. Rattanachomsria, T. Laothanachareona, L.
Eurwilaichitra, Y. Igarashib, and V. Champredaa, “Analysis of a
thermophilic lignocellulose degrading microbial consortium and
multi-species lignocellulolytic enzyme system,” Enzyme and Microb
Tech, vol. 47, 2010, pp. 283–290.
[6] V.A. Vavilina, B. Fernandezb, J. Palatsib, and X. Flotatsb,
“Hydrolysis kinetics in anaerobic degradation of particulate organic
material: An overview,” Waste Manage, vol. 28, 2008, pp. 939-951.
[7] N. Mosiera, C. Wymanb, B.Dalec, R. Elanderd, Y.Y. Leee, M.
Holtzapplef, and M. Ladischa, “Features of promising technologies for pretreatment of lignocellulosic biomass,” Bioresour Technol, vol.
96, 2005, pp. 673–686.
[8] S. Soundar, and T. S. Chandra, “Cellulose degradation by a mixed
bacterial culture,” J Ind Microbiol, Vol. 2, 1987, pp. 257–265.
[9] M. de la Torre, and C. C. Campillo, “Isolation and characterization of
a symbiotic cellulolytic mixed bacterial culture,” Appl Microbiol
Biotechnol, vol. 19, 1984, pp. 430–434.
[10] S. Haruta, Z. Cui, Z. Huang, M. Li, M. Ishii, and Y. Igarashi,
“Construction of a stable microbial community with high cellulosedegradation
ability,” Appl Microbiol Biotechnol, vol. 59, 2002, pp.
529–534.
[11] X. Yuan, Y. Cao, J. Li, B. Wen, W. Zhu, X. Wang, and Z. Cui,
“Effect of pretreatment by a microbial consortium on methane
production of waste paper and cardboard,” Bioresour Technol, vol.
118, 2012, pp. 281–288.
[12] Y. Gu, X. Chen,mj Z. Liu, X. Zhou, and Y. Zhang, “Effect of
inoculum sources on the anaerobic digestion of rice straw,” Bioresour
Technol, vol. 158, 2014, pp. 149–155.
[13] Q. Zhang, J. He, M. Tian, Z. Maoa, L. Tang, J. Zhang, and H. Zhang,
“Enhancement of methane production from cassava residues by
biological pretreatment using a constructed microbial consortium,”
Bioresour Technol, vol. 102, 2011, pp. 8899-8906.
[14] B. Wen, X. Yuan, Y. Cao, Y. Liu, X. Wang, and Z. Cui,
“Optimization of liquid fermentation of microbial consortium WSD-5
followed by saccharification and acidification of wheat straw,”
Bioresour Technol, vol. 118, 2012, pp. 141-149.
[15] G. L. Miller, “Use of dinitosalicylic acid reagent for determination of
reducing sugas,” Anal Chem, vol. 47, 1959, pp. 426-428.
[16] V. Shahhrmma, H. Vij, P. K. Singh, and S. Bhatt, “Potential Cellulase
Production, Optimization and Saccharification Study by Novel
Thermophilic Microbes,” Sustain Biotechnol, vol. 1,2013, pp.19-26.
[17] H.Y. Yang, H. Wu, X. Wang, Z. Cui, and Y. H. Li, “Selection and
characteristic of a switch grass-colonizing microbial community to
produce extracellular cellulose and xylanases,” Bioresour Technol,
vol. 102, 2011, pp. 3546-3550.
[18] R. Chandra, H. Takeuchi, and T. Hasegawa, "Hydrothermal
pretreatment of rice straw biomass: A potential and promising method
for enhanced methane production" Appli Energ, vol. 94, 2012, pp.
129-140
[19] U. G. Phutela, and N. Sahni, "Effect ofFusarium sp. on paddy straw
digestibility and biogas production", J Adv Lab Res in Biol, vol. 3,
2012 pp. 9-12.
[1] L. A. Lucia, “Lignocellulosic biomass: Replace petroleum,” Raleigh,
N.C., 2008, pp.981-982.
[2] Data Retrieved from the link: www.irri.org
[3] Data Retrieved from the link: www.dede.go.th
[4] P. S. Nigam, N. Gupta, and A. Anthwal, “Pre-treatment of agroindustrial
residues,” in Biotechnology for agro-industrial residues
utilization, 1st ed. Netherlands: Springer, 2009, pp. 13-33.
[5] S. Wongwilaiwalina, U. Rattanachomsria, T. Laothanachareona, L.
Eurwilaichitra, Y. Igarashib, and V. Champredaa, “Analysis of a
thermophilic lignocellulose degrading microbial consortium and
multi-species lignocellulolytic enzyme system,” Enzyme and Microb
Tech, vol. 47, 2010, pp. 283–290.
[6] V.A. Vavilina, B. Fernandezb, J. Palatsib, and X. Flotatsb,
“Hydrolysis kinetics in anaerobic degradation of particulate organic
material: An overview,” Waste Manage, vol. 28, 2008, pp. 939-951.
[7] N. Mosiera, C. Wymanb, B.Dalec, R. Elanderd, Y.Y. Leee, M.
Holtzapplef, and M. Ladischa, “Features of promising technologies for pretreatment of lignocellulosic biomass,” Bioresour Technol, vol.
96, 2005, pp. 673–686.
[8] S. Soundar, and T. S. Chandra, “Cellulose degradation by a mixed
bacterial culture,” J Ind Microbiol, Vol. 2, 1987, pp. 257–265.
[9] M. de la Torre, and C. C. Campillo, “Isolation and characterization of
a symbiotic cellulolytic mixed bacterial culture,” Appl Microbiol
Biotechnol, vol. 19, 1984, pp. 430–434.
[10] S. Haruta, Z. Cui, Z. Huang, M. Li, M. Ishii, and Y. Igarashi,
“Construction of a stable microbial community with high cellulosedegradation
ability,” Appl Microbiol Biotechnol, vol. 59, 2002, pp.
529–534.
[11] X. Yuan, Y. Cao, J. Li, B. Wen, W. Zhu, X. Wang, and Z. Cui,
“Effect of pretreatment by a microbial consortium on methane
production of waste paper and cardboard,” Bioresour Technol, vol.
118, 2012, pp. 281–288.
[12] Y. Gu, X. Chen,mj Z. Liu, X. Zhou, and Y. Zhang, “Effect of
inoculum sources on the anaerobic digestion of rice straw,” Bioresour
Technol, vol. 158, 2014, pp. 149–155.
[13] Q. Zhang, J. He, M. Tian, Z. Maoa, L. Tang, J. Zhang, and H. Zhang,
“Enhancement of methane production from cassava residues by
biological pretreatment using a constructed microbial consortium,”
Bioresour Technol, vol. 102, 2011, pp. 8899-8906.
[14] B. Wen, X. Yuan, Y. Cao, Y. Liu, X. Wang, and Z. Cui,
“Optimization of liquid fermentation of microbial consortium WSD-5
followed by saccharification and acidification of wheat straw,”
Bioresour Technol, vol. 118, 2012, pp. 141-149.
[15] G. L. Miller, “Use of dinitosalicylic acid reagent for determination of
reducing sugas,” Anal Chem, vol. 47, 1959, pp. 426-428.
[16] V. Shahhrmma, H. Vij, P. K. Singh, and S. Bhatt, “Potential Cellulase
Production, Optimization and Saccharification Study by Novel
Thermophilic Microbes,” Sustain Biotechnol, vol. 1,2013, pp.19-26.
[17] H.Y. Yang, H. Wu, X. Wang, Z. Cui, and Y. H. Li, “Selection and
characteristic of a switch grass-colonizing microbial community to
produce extracellular cellulose and xylanases,” Bioresour Technol,
vol. 102, 2011, pp. 3546-3550.
[18] R. Chandra, H. Takeuchi, and T. Hasegawa, "Hydrothermal
pretreatment of rice straw biomass: A potential and promising method
for enhanced methane production" Appli Energ, vol. 94, 2012, pp.
129-140
[19] U. G. Phutela, and N. Sahni, "Effect ofFusarium sp. on paddy straw
digestibility and biogas production", J Adv Lab Res in Biol, vol. 3,
2012 pp. 9-12.
@article{"International Journal of Biological, Life and Agricultural Sciences:69806", author = "Supanun Kangrang and Kraipat Cheenkachorn and Kittiphong Rattanaporn and Malinee Sriariyanun", title = "Analysis of a Lignocellulose Degrading Microbial Consortium to Enhance the Anaerobic Digestion of Rice Straws", abstract = "Rice straw is lignocellulosic biomass which can be utilized as substrate for the biogas production. However, due to the property and composition of rice straw, it is difficult to be degraded by hydrolysis enzymes. One of the pretreatment methods that modify such properties of lignocellulosic biomass is the application of lignocellulose-degrading microbial consortia. The aim of this study is to investigate the effect of microbial consortia to enhance biogas production. To select the high efficient consortium, cellulase enzymes were extracted and their activities were analyzed. The results suggested that microbial consortium culture obtained from cattle manure is the best candidate compared to decomposed wood and horse manure. A microbial consortium isolated from cattle manure was then mixed with anaerobic sludge and used as inoculum for biogas production. The optimal conditions for biogas production were investigated using response surface methodology (RSM). The tested parameters were the ratio of amount of microbial consortium isolated and amount of anaerobic sludge (MI:AS), substrate to inoculum ratio (S:I) and temperature. Here, the value of the regression coefficient R2 = 0.7661 could be explained by the model which is high to advocate the significance of the model. The highest cumulative biogas yield was 104.6 ml/g-rice straw at optimum ratio of MI:AS, ratio of S:I, and temperature of 2.5:1, 15:1 and 44°C respectively.
", keywords = "Lignocellulolytic biomass, microbial consortium,
cellulase, biogas, Response Surface Methodology.
", volume = "9", number = "5", pages = "498-5", }
