Abstract: The increasing generation of saline wastewater through various industrial activities is becoming a global concern for activated sludge (AS) based biological treatment which is widely applied in wastewater treatment plants (WWTPs). As for the AS process, an increase in wastewater salinity has negative impact on its overall performance. The advent of conventional aerobic granular sludge (AGS) or bacterial AGS biotechnology has gained much attention because of its superior performance. The development of algal-bacterial AGS could enhance better nutrients removal, potentially reduce aeration cost through symbiotic algae-bacterial activity, and thus, can also reduce overall treatment cost. Nonetheless, the potential of salt stress to decrease biomass growth, microbial activity and nutrient removal exist. Up to the present, little information is available on saline wastewater treatment by algal-bacterial AGS. To the authors’ best knowledge, a comparison of the two AGS systems has not been done to evaluate nutrients removal capacity in the context of salinity increase. This study sought to figure out the impact of salinity on the algal-bacterial AGS system in comparison to bacterial AGS one, contributing to the application of AGS technology in the real world of saline wastewater treatment. In this study, the salt concentrations tested were 0 g/L, 1 g/L, 5 g/L, 10 g/L and 15 g/L of NaCl with 24-hr artificial illuminance of approximately 97.2 µmol m¯²s¯¹, and mature bacterial and algal-bacterial AGS were used for the operation of two identical sequencing batch reactors (SBRs) with a working volume of 0.9 L each, respectively. The results showed that salinity increase caused no apparent change in the color of bacterial AGS; while for algal-bacterial AGS, its color was progressively changed from green to dark green. A consequent increase in granule diameter and fluffiness was observed in the bacterial AGS reactor with the increase of salinity in comparison to a decrease in algal-bacterial AGS diameter. However, nitrite accumulation peaked from 1.0 mg/L and 0.4 mg/L at 1 g/L NaCl in the bacterial and algal-bacterial AGS systems, respectively to 9.8 mg/L in both systems when NaCl concentration varied from 5 g/L to 15 g/L. Almost no ammonia nitrogen was detected in the effluent except at 10 g/L NaCl concentration, where it averaged 4.2 mg/L and 2.4 mg/L, respectively, in the bacterial and algal-bacterial AGS systems. Nutrients removal in the algal-bacterial system was relatively higher than the bacterial AGS in terms of nitrogen and phosphorus removals. Nonetheless, the nutrient removal rate was almost 50% or lower. Results show that algal-bacterial AGS is more adaptable to salinity increase and could be more suitable for saline wastewater treatment. Optimization of operation conditions for algal-bacterial AGS system would be important to ensure its stably high efficiency in practice.
Abstract: Synthetic domestic wastewater was treated via combining treatment methods, including electrochemical oxidation, adsorption, and sequencing batch reactor (SBR). In the upper part of the reactor, an anode and a cathode (Ti/RuO2-IrO2) were organized in parallel for the electrochemical oxidation procedure. Sodium sulfate (Na2SO4) with a concentration of 2.5 g/L was applied as the electrolyte. The voltage and current were fixed on 7.50 V and 0.40 A, respectively. Then, 15% working value of the reactor was filled by activated sludge, and 85% working value of the reactor was added with synthetic wastewater. Powdered cockleshell, 1.5 g/L, was added in the reactor to do ion-exchange. Response surface methodology was employed for statistical analysis. Reaction time (h) and pH were considered as independent factors. A total of 97.0% biochemical oxygen demand, 99.9% phosphorous and 88.6% cadmium were eliminated at the optimum reaction time (80.0 min) and pH (6.4).
Abstract: Worldwide, about two-thirds of industrial and domestic wastewater effluent is discharged without treatment, which can cause contamination and eutrophication of the water. In particular, for Mediterranean countries, irrigation with treated wastewater would mitigate the water stress and support the agricultural sector. Changing global weather patterns will make the situation worse, due to increased susceptibility to drought, which can cause major environmental, social, and economic problems. The study was carried out in open field in an intensive agricultural area of the Apulian region in Southern Italy where freshwater resources are often scarce. As well as providing a water resource, irrigation with treated wastewater represents a significant source of nutrients for soil–plant systems. However, the use of wastewater might have further effects on soil. This study thus investigated the long-term impact of irrigation with reclaimed agro-industrial wastewater on the chemical characteristics of the soil. Two crops (processing tomato and broccoli) were cultivated in succession in Stornarella (Foggia) over four years from 2012 to 2016 using two types of irrigation water: groundwater and tertiary treated agro-industrial wastewater that had undergone an activated sludge process, sedimentation filtration, and UV radiation. Chemical analyses were performed on the irrigation waters and soil samples. The treated wastewater was characterised by high levels of several chemical parameters including TSS, EC, COD, BOD5, Na+, Ca2+, Mg2+, NH4-N, PO4-P, K+, SAR and CaCO3, as compared with the groundwater. However, despite these higher levels, the mean content of several chemical parameters in the soil did not show relevant differences between the irrigation treatments, in terms of the chemical features of the soil.
Abstract: Nitrification is essential to biological processes
designed to remove ammonia and/or total nitrogen. It removes excess
nitrogenous compound in wastewater which could be very toxic to
the aquatic fauna or cause serious imbalance of such aquatic
ecosystem. Efficient nitrification is linked to an in-depth knowledge
of the structure and dynamics of the nitrifying community structure
within the wastewater treatment systems. In this study, molecular
technique was employed for characterizing the microbial structure of
activated sludge [ammonia oxidizing bacteria (AOB) and nitrite
oxidizing bacteria (NOB)] in a municipal wastewater treatment with
intention of linking it to the plant efficiency. PCR based phylogenetic
analysis was also carried out. The average operating and
environmental parameters as well as specific nitrification rate of plant
was investigated during the study. During the investigation the average temperature was 23±1.5oC.
Other operational parameters such as mixed liquor suspended solids
and chemical oxygen demand inversely correlated with ammonia
removal. The dissolved oxygen level in the plant was constantly
lower than the optimum (between 0.24 and 1.267 mg/l) during this
study. The plant was treating wastewater with influent ammonia
concentration of 31.69 and 24.47 mg/L. The influent flow rates
(ML/Day) was 96.81 during period. The dominant nitrifiers include:
Nitrosomonas spp. Nitrobacter spp. and Nitrospira spp. The AOB
had correlation with nitrification efficiency and temperature. This
study shows that the specific ammonia oxidizing rate and the specific
nitrate formation rates can serve as good indicator of the plant overall
nitrification performance.
Abstract: Anammox is a novel and promising technology that has changed the traditional concept of biological nitrogen removal. The process facilitates direct oxidation of ammonical nitrogen under anaerobic conditions with nitrite as an electron acceptor without addition of external carbon sources. The present study investigated the feasibility of Anammox Hybrid Reactor (AHR) combining the dual advantages of suspended and attached growth media for biodegradation of ammonical nitrogen in wastewater. Experimental unit consisted of 4 nos. of 5L capacity AHR inoculated with mixed seed culture containing anoxic and activated sludge (1:1). The process was established by feeding the reactors with synthetic wastewater containing NH4-H and NO2-N in the ratio 1:1 at HRT (hydraulic retention time) of 1 day. The reactors were gradually acclimated to higher ammonium concentration till it attained pseudo steady state removal at a total nitrogen concentration of 1200 mg/l. During this period, the performance of the AHR was monitored at twelve different HRTs varying from 0.25-3.0 d with increasing NLR from 0.4 to 4.8 kg N/m3d. AHR demonstrated significantly higher nitrogen removal (95.1%) at optimal HRT of 1 day. Filter media in AHR contributed an additional 27.2% ammonium removal in addition to 72% reduction in the sludge washout rate. This may be attributed to the functional mechanism of filter media which acts as a mechanical sieve and reduces the sludge washout rate many folds. This enhances the biomass retention capacity of the reactor by 25%, which is the key parameter for successful operation of high rate bioreactors. The effluent nitrate concentration, which is one of the bottlenecks of anammox process was also minimised significantly (42.3-52.3 mg/L). Process kinetics was evaluated using first order and Grau-second order models. The first-order substrate removal rate constant was found as 13.0 d-1. Model validation revealed that Grau second order model was more precise and predicted effluent nitrogen concentration with least error (1.84±10%). A new mathematical model based on mass balance was developed to predict N2 gas in AHR. The mass balance model derived from total nitrogen dictated significantly higher correlation (R2=0.986) and predicted N2 gas with least error of precision (0.12±8.49%). SEM study of biomass indicated the presence of heterogeneous population of cocci and rod shaped bacteria of average diameter varying from 1.2-1.5 mm. Owing to enhanced NRE coupled with meagre production of effluent nitrate and its ability to retain high biomass, AHR proved to be the most competitive reactor configuration for dealing with nitrogen laden wastewater.
Abstract: Despite of few research works on municipal sludge, still there is a lack of actual data. Thus, this work was focused on the conditioning and rheology of fresh activated sludge. The effect of cationic polyelectrolyte has been investigated at different concentrations and pH values in a comparative fashion. Yield stress is presented in all results indicating the minimum stress that necessary to reach flow conditions. Connections between particle-particle is the reason for this yield stress, also, the addition of polyelectrolyte causes strong bonds between particles and water resulting in the aggregation of particles which required higher shear stress in order to flow. The results from the experiments indicate that the cationic polyelectrolytes have significant effluence on the sludge characteristic and water quality such as turbidity, SVI, zone settling rate and shear stress.
Abstract: 40L of hollow fiber membrane bioreactor with solids retention times (SRT) of 30, 15 and 4 days were setup for treating synthetic wastewater at hydraulic retention times (HRT) of 12, 8 and 4 hours. The objectives of the study were to investigate the effects of SRT and HRT on membrane fouling. A comparative analysis was carried out for physiochemical quality parameters (turbidity, suspended solids, COD, NH3-N and PO43-). Scanning electron microscopy (SEM), energy diffusive X-ray (EDX) analyzer and particle size distribution (PSD) were used to characterize the membrane fouling properties. The influence of SRT on the quality of effluent, activated sludge quality, and membrane fouling were also correlated. Lower membrane fouling and slower rise in trans-membrane pressure (TMP) were noticed at the longest SRT and HRT of 30d and 12h, respectively. Increasing SRT results in noticeable reduction of dissolved organic matters. The best removal efficiencies of COD, TSS, NH3-N and PO43- were 93%, 98%, 80% and 30% respectively. The high HRT with shorter SRT induced faster fouling rate. The main fouling resistance was cake layer. The most severe membrane fouling was observed at SRT and HRT of 4 and 12, respectively with thickness cake layer of 17mm as reflected by higher TMP, lower effluent removal and thick sludge cake layer.
Abstract: The heavy metals pollution in water, sediments and fish of Lake Manzala affected form the disposal of wastewater, industrial and agricultural drainage water into the lake on the environmental situation. A pilot plant with an industrial discharge flow of 135L/h designed according to the activated sludge plant to simulate between the biological and chemical treatment with the addition of alum to the aeration tank with dosages of 100, 150, 200 and 250 mg/L. The industrial discharge had concentrations of Lead and BOD5 with an average range 1.22, 145mg/L respectively. That means the average Pb was high up to 25 times than the allowed permissible concentration. The optimization of the chemical-biological process using 200mg/L Alum dosage compared has improvement of Lead and BOD5 removal efficiency to 61.76% and 56% respectively.
Abstract: The aim of the study is to improve removal of trace organic contaminants dissolved in activated sludge by the process of filtration with membrane bioreactor combined with modified activated carbon, for a maximum removal of organic compounds characterized by low molecular weight. Special treatment was conducted in laboratory on activated carbon. Tow reaction parameters: the pH of aqueous middle and the type of granular activated carbon were very important to improve the removal and to motivate the electrostatic Interactions of organic compounds with modified activated carbon in addition to physical adsorption, ligand exchange or complexation on the surface activated carbon. The results indicate that modified activated carbon has a strong impact in removal 21 of organic contaminants and in percentage of 100% of the process.
Abstract: The effect of polyelectrolytes; cationic and anionic charges and coagulants have been investigated for fresh activated sludge at different concentrations and pH values in a comparative fashion. The results from the experiments indicate that the cationic polyelectrolytes have a significant effluence on the sludge characteristic, degree of flocculation and water quality such as turbidity and SVI. The results show that the cationic CPAM-80 is the most effective polyelectrolyte used corresponding to turbidity and SVI despite of the variations in feed properties of the fresh activated sludge.
Abstract: Ever since industrial revolution began, our ecosystem
has changed. And indeed, the negatives outweigh the positives.
Industrial waste usually released into all kinds of body of water, such
as river or sea. Tempeh waste is one example of waste that carries
many hazardous and unwanted substances that will affect the
surrounding environment. Tempeh is a popular fermented food in
Asia which is rich in nutrients and active substances. Tempeh liquid
waste- in particular- can cause an air pollution, and if penetrates
through the soil, it will contaminates ground-water, making it
unavailable for the water to be consumed. Moreover, bacteria will
thrive within the polluted water, which often responsible for causing
many kinds of diseases. The treatment used for this chemical waste is
biological treatment such as constructed wetland and activated
sludge. These kinds of treatment are able to reduce both physical and
chemical parameters altogether such as temperature, TSS, pH, BOD,
COD, NH3-N, NO3-N, and PO4-P. These treatments are implemented
before the waste is released into the water. The result is a
comparation between constructed wetland and activated sludge,
along with determining which method is better suited to reduce the
physical and chemical subtances of the waste.
Abstract: Statistics Canada stated that the wastewater treatment
facilities in most provinces are aging and passes 63% of their useful
life in 2007 the highest ratio among public infrastructure assets.
Currently, there is no standard condition rating system for wastewater
treatment plants that give a specific rating index that describe the
physical integrity of different infrastructure elements in the treatment
plant and its environmental performance. The main objective of this
study is to develop a condition-rating index for wastewater treatment
plants mainly activated sludge systems. The proposed WWTP CRI, is
based on dividing the treatment plant into its three treatment phases;
primary phase, secondary phase and the tertiary phase. The
condition-rating index will reflect the infrastructures state for each
phase, mainly tanks, pipes, blowers and pumps.
Abstract: A shaft-type activated sludge reactor has been
developed in order to study the feasibility of high-rate wastewater
treatment. The reactor having volume of about 14.5 L was operated
with the acclimated mixed activated sludge under batch and
continuous mode using a synthetic wastewater as feed. The batch
study was performed with varying chemical oxygen demand (COD)
concentrations of 1000–3500 mg·L-1 for a batch period up to 9 h. The
kinetic coefficients: Ks, k, Y and kd were obtained as 2040.2 mg·L-1
and 0.105 h-1, 0.878 and 0.0025 h-1 respectively from Monod-s
approach. The continuous study showed a stable and steady state
operation for a hydraulic retention time (HRT) of 8 h and influent
COD of about 1000 mg·L-1. A maximum COD removal efficiency of
about 80% was attained at a COD loading rate and food-tomicroorganism
(F/M) ratio (COD basis) of 3.42 kg·m-3d-1 and 1.0
kg·kg-1d-1 respectively under a HRT of 8 h. The reactor was also
found to handle COD loading rate and F/M ratio of 10.8 kg·m-3d-1
and 2.20 kg·kg-1d-1 respectively showing a COD removal efficiency
of about 46%.
Abstract: In the present study, an attempt was made to examine
the potential of aerobic mixed culture for decolourization of Remazol Black B dye in batch reactors. The effect of pH, temperature, inoculum, initial concentration of dye and initial concentration of
glucose was studied with an aim to determine the optimal conditions
required for maximum decolourization and degradation. The culture
exhibited maximum decolourization ability at pH between 7-8 and at
30°C. A 10% (v/v) inoculum and 1% (w/v) glucose concentration
were found to be the optimum for decolourization. A maximum of
98% decolourization was observed at 25 ppm initial concentration of
dye after 18 hours of incubation period. At higher dye concentration
of 300 ppm, the removal in colour was found to be 75% in 48 hours of incubation period. The results show that the enriched mixed culture from activated sludge has good potential in removal of Remazol Black B dye from wastewater under aerobic conditions.
Abstract: The pollutant removal efficiency of the Intermittently
Decanted Extended Aeration (IDEA) wastewater treatment system at
Curtin University Sarawak Campus, and conventional activated
sludge wastewater treatment system at a local resort, Resort A, is
monitored. The influent and effluent characteristics are tested during
wet and dry weather conditions, and peak and off peak periods. For
the wastewater treatment systems at Curtin Sarawak and Resort A,
during dry weather and peak season, it was found that the BOD5
concentration in the influent is 121.7mg/L and 80.0mg/L
respectively, and in the effluent, 18.7mg/L and and 18.0mg/L
respectively. Analysis of the performance of the IDEA treatment
system showed that the operational costs can be minimized by 3%, by
decreasing the number of operating cycles. As for the treatment
system in Resort A, by utilizing a smaller capacity air blower, a
saving of 12% could be made in the operational costs.
Abstract: In this study, communities of ammonia-oxidizing
archaea (AOA) and ammonia-oxidizing bacteria (AOB) in nitrifying
activated sludge (NAS) prepared by enriching sludge from a
municipal wastewater treatment plant in three continuous-flow
reactors receiving an inorganic medium containing different
ammonium concentrations of 2, 10, and 30 mM NH4
+-N (NAS2,
NAS10, and NAS30, respectively) were investigated using molecular
analysis. Results suggested that almost all AOA clones from NAS2,
NAS10, and NAS30 fell into the same AOA cluster and AOA
communities in NAS2 and NAS10 were more diverse than those of
NAS30. In contrast to AOA, AOB communities obviously shifted
from the seed sludge to enriched NASs and in each enriched NAS,
communities of AOB varied particularly. The seed sludge contained
members of N. communis cluster and N. oligotropha cluster. After it
was enriched under various ammonium loads, members of N.
communis cluster disappeared from all enriched NASs. AOB with
high affinity to ammonia presented in NAS 2, AOB with low affinity
to ammonia presented in NAS 30, and both types of AOB survived in
NAS 10. These demonstrated that ammonium load significantly
influenced AOB communities, but not AOA communities in enriched
NASs.
Abstract: A local wastewater treatment plant (WWTP)
experiencing poor nitrification tracked down high level of
surfactants in the plant-s influent and effluent. The aims of this project were to assess the potential inhibitory effect of surfactants on activated sludge processes. The effect of the
presence of TergitolNP-9, TrigetolNP-7, Trigetol15-S-9,
dodecylbenzene sulphonate (SDBS) and sodium dodecyl
sulfate (SDS) on activated sludge oxygen uptake rate (OUR) and nitrification were assessed. The average concentration of non-ionic and anionic
surfactants in the influent to the local WWTP were 7 and 8.7 mg/L, respectively. Removal of 67% to 90% of the non-ionic and 93-99% of the anionic surfactants tested were measured. All surfactants tested showed inhibitory effects both on OUR
and nitrification. SDS incurred the lowest inhibition whereas
SDBS and NP-9 caused severe inhibition to OUR and
Nitrification. Activated sludge flocs sizes slightly decreased
after 3 hours contact with the surfactant present in the test.
The results obtained indicated that high concentrations of
surfactants are likely to have an adverse effect on the
performance of WWTPs utilizing activated sludge processes.
Abstract: 17α-ethinylestradiol (EE2) is a recalcitrant micropollutant which is found in small amounts in municipal wastewater. But these small amounts still adversely affect for the reproductive function of aquatic organisms. Evidence in the past suggested that full-scale WWTPs equipped with nitrification process enhanced the removal of EE2 in the municipal wastewater. EE2 has been proven to be able to be transformed by ammonia oxidizing bacteria (AOB) via co-metabolism. This research aims to clarify the EE2 degradation pattern by different consortium of ammonia oxidizing microorganism (AOM) including AOA (ammonia oxidizing archaea) and investigate contribution between the existing ammonia monooxygenase (AMO) and new synthesized AOM. The result showed that AOA or AOB of N. oligotropha cluster in enriched nitrifying activated sludge (NAS) from 2mM and 5mM, commonly found in municipal WWTPs, could degrade EE2 in wastewater via co-metabolism. Moreover, the investigation of the contribution between the existing ammonia monooxygenase (AMO) and new synthesized AOM demonstrated that the new synthesized AMO enzyme may perform ammonia oxidation rather than the existing AMO enzyme or the existing AMO enzyme may has a small amount to oxidize ammonia.
Abstract: Thirty six samples from each (aerobic and anoxic)
activated sludge were collected from two wastewater treatment plants
with MBRs in Berlin, Germany. The samples were prepared for count
and definition of fungal isolates; these isolates were purified by
conventional techniques and identified by microscopic examination.
Sixty tow species belonging to 28 genera were isolated from
activated sludge samples under aerobic conditions (28 genera and 58
species) and anoxic conditions (26 genera and 52 species). The
obtained data show that, Aspergillus was found at 94.4% followed by
Penicillium 61.1 %, Fusarium (61.1 %), Trichoderma (44.4 %) and
Geotrichum candidum (41.6 %) species were the most prevalent in all
activated sludge samples. The study confirmed that fungi can thrive
in activated sludge and sporulation, but isolated in different numbers
depending on the effect of aeration system. Some fungal species in
our study are saprophytic, and other a pathogenic to plants and
animals.
Abstract: The purpose of the experiments described in this article was the comparison of integrated fixed film activated sludge (IFAS) and activated sludge (AS) system. The IFAS applied system consists of the cigarette filter rods (wasted filter in tobacco factories) as a biofilm carrier. The comparison with activated sludge was performed by two parallel treatment lines. Organic substance, ammonia and TP removal was investigated over four month period. Synthetic wastewater was prepared with ordinary tap water and glucose as the main sources of carbon and energy, plus balanced macro and micro nutrients. COD removal percentages of 94.55%, and 81.62% were achieved for IFAS and activated sludge system, respectively. Also, ammonia concentration significantly decreased by increasing the HRT in both systems. The average ammonia removal of 97.40 % and 96.34% were achieved for IFAS and activated sludge system, respectively. The removal efficiency of total phosphorus (TP-P) was 60.64%, higher than AS process by 56.63% respectively.