Abstract: Nitrification is essential to biological processes
designed to remove ammonia and/or total nitrogen. It removes excess
nitrogenous compound in wastewater which could be very toxic to
the aquatic fauna or cause serious imbalance of such aquatic
ecosystem. Efficient nitrification is linked to an in-depth knowledge
of the structure and dynamics of the nitrifying community structure
within the wastewater treatment systems. In this study, molecular
technique was employed for characterizing the microbial structure of
activated sludge [ammonia oxidizing bacteria (AOB) and nitrite
oxidizing bacteria (NOB)] in a municipal wastewater treatment with
intention of linking it to the plant efficiency. PCR based phylogenetic
analysis was also carried out. The average operating and
environmental parameters as well as specific nitrification rate of plant
was investigated during the study. During the investigation the average temperature was 23±1.5oC.
Other operational parameters such as mixed liquor suspended solids
and chemical oxygen demand inversely correlated with ammonia
removal. The dissolved oxygen level in the plant was constantly
lower than the optimum (between 0.24 and 1.267 mg/l) during this
study. The plant was treating wastewater with influent ammonia
concentration of 31.69 and 24.47 mg/L. The influent flow rates
(ML/Day) was 96.81 during period. The dominant nitrifiers include:
Nitrosomonas spp. Nitrobacter spp. and Nitrospira spp. The AOB
had correlation with nitrification efficiency and temperature. This
study shows that the specific ammonia oxidizing rate and the specific
nitrate formation rates can serve as good indicator of the plant overall
nitrification performance.
Abstract: 17α-ethinylestradiol (EE2) is a recalcitrant micropollutant which is found in small amounts in municipal wastewater. But these small amounts still adversely affect for the reproductive function of aquatic organisms. Evidence in the past suggested that full-scale WWTPs equipped with nitrification process enhanced the removal of EE2 in the municipal wastewater. EE2 has been proven to be able to be transformed by ammonia oxidizing bacteria (AOB) via co-metabolism. This research aims to clarify the EE2 degradation pattern by different consortium of ammonia oxidizing microorganism (AOM) including AOA (ammonia oxidizing archaea) and investigate contribution between the existing ammonia monooxygenase (AMO) and new synthesized AOM. The result showed that AOA or AOB of N. oligotropha cluster in enriched nitrifying activated sludge (NAS) from 2mM and 5mM, commonly found in municipal WWTPs, could degrade EE2 in wastewater via co-metabolism. Moreover, the investigation of the contribution between the existing ammonia monooxygenase (AMO) and new synthesized AOM demonstrated that the new synthesized AMO enzyme may perform ammonia oxidation rather than the existing AMO enzyme or the existing AMO enzyme may has a small amount to oxidize ammonia.