Abstract: Liposomal magnetofection is the most powerful nonviral method for the nucleic acid delivery into the cultured cancer cells and widely used for in vitro applications. Use of the static magnetic field condition may result in non-uniform distribution of aggregate complexes on the surface of cultured cells. To prevent this, we developed the new device which allows to concentrate aggregate complexes under dynamic magnetic field, assisting more contact of these complexes with cellular membrane and, possibly, stimulating endocytosis. Newly developed device for magnetofection under dynamic gradient magnetic field, “DynaFECTOR", was used to compare transfection efficiency of human liver hepatocellular carcinoma cell line HepG2 with that obtained by lipofection and magnetofection. The effect of two parameters on transfection efficiency, incubation time under dynamic magnetic field and rotation frequency of magnet, was estimated. Liposomal magnetofection under dynamic gradient magnetic field showed the highest transfection efficiency for HepG2 cells.
Abstract: Knowledge Discovery in Databases (KDD) has
evolved into an important and active area of research because of
theoretical challenges and practical applications associated with the
problem of discovering (or extracting) interesting and previously
unknown knowledge from very large real-world databases. Rough
Set Theory (RST) is a mathematical formalism for representing
uncertainty that can be considered an extension of the classical set
theory. It has been used in many different research areas, including
those related to inductive machine learning and reduction of
knowledge in knowledge-based systems. One important concept
related to RST is that of a rough relation. In this paper we presented
the current status of research on applying rough set theory to KDD,
which will be helpful for handle the characteristics of real-world
databases. The main aim is to show how rough set and rough set
analysis can be effectively used to extract knowledge from large
databases.
Abstract: Availability of high dimensional biological datasets such as from gene expression, proteomic, and metabolic experiments can be leveraged for the diagnosis and prognosis of diseases. Many classification methods in this area have been studied to predict disease states and separate between predefined classes such as patients with a special disease versus healthy controls. However, most of the existing research only focuses on a specific dataset. There is a lack of generic comparison between classifiers, which might provide a guideline for biologists or bioinformaticians to select the proper algorithm for new datasets. In this study, we compare the performance of popular classifiers, which are Support Vector Machine (SVM), Logistic Regression, k-Nearest Neighbor (k-NN), Naive Bayes, Decision Tree, and Random Forest based on mock datasets. We mimic common biological scenarios simulating various proportions of real discriminating biomarkers and different effect sizes thereof. The result shows that SVM performs quite stable and reaches a higher AUC compared to other methods. This may be explained due to the ability of SVM to minimize the probability of error. Moreover, Decision Tree with its good applicability for diagnosis and prognosis shows good performance in our experimental setup. Logistic Regression and Random Forest, however, strongly depend on the ratio of discriminators and perform better when having a higher number of discriminators.
Abstract: Continuation of an active call is one of the most important quality measurements in the cellular systems. Handoff process enables a cellular system to provide such a facility by transferring an active call from one cell to another. Different approaches are proposed and applied in order to achieve better handoff service. The principal parameters used to evaluate handoff techniques are: forced termination probability and call blocking probability. The mechanisms such as guard channels and queuing handoff calls decrease the forced termination probability while increasing the call blocking probability. In this paper we present an overview about the issues related to handoff initiation and decision and discuss about different types of handoff techniques available in the literature.
Abstract: One of the essential requirements in order to have a
realistic surgical simulator is real-time interaction by means of a
haptic interface is. In fact, reproducing haptic sensations increases
the realism of the simulation. However, the interaction need to be
performed in real-time, since a delay between the user action and the
system reaction reduces the user immersion. In this paper, we present
a prototype of the coronary stent implant simulator developed in the
HERMES Project; this system allows real-time interactions with a
artery by means of a specific haptic device; thus the user can
interactively navigate in a reconstructed artery and force feedback is
produced when contact occurs between the artery walls and the
medical instruments
Abstract: Fourty one strains of ESBL producing P.aeruginosa
which were previously isolated from burn patients in Kerman
University general hospital, Iran were subjected to PCR, RFLP and
sequencing in order to determine the type of extended spectrum β-
lactamases (ESBL), the restriction digestion pattern and possibility of
mutation among detected genes. DNA extraction was carried out by
phenol chloroform method. PCR for detection of bla genes was
performed using specific primer for each gene. Restriction Fragment
Length Polymorphism (RFLP) for ESBL genes was carried out using
EcoRI, NheI, PVUII, EcoRV, DdeI, and PstI restriction enzymes. The
PCR products were subjected to direct sequencing of both the strands
for identification of the ESBL genes.The blaCTX-M, blaVEB-1, blaPER-1,
blaGES-1, blaOXA-1, blaOXA-4 and blaOXA-10 genes were detected in the
(n=1) 2.43%, (n=41)100%, (n=28) 68.3%, (n=10) 24.4%, (n=29)
70.7%, (n=7)17.1% and (n=38) 92.7% of the ESBL producing isolates
respectively. The RFLP analysis showed that each ESBL gene has
identical pattern of digestion among the isolated strains. Sequencing
of the ESBL genes confirmed the genuinety of PCR products and
revealed no mutation in the restriction sites of the above genes. From
results of the present investigation it can be concluded that blaVEB-1
and blaCTX-M were the most and the least frequently isolated ESBL
genes among the P.aeruginosa strains isolated from burn patients. The
RFLP and sequencing analysis revealed that same clone of the bla
genes were indeed existed among the antibiotic resistant strains.
Abstract: A simple impedance matching technique for inset feed
grooved microstrip patch antenna based on the concept of coplanar
waveguide feed line has been developed and investigated for a
printed antenna at X-Band frequency of 10GHz. The proposed
technique has been used in the design of Linear Grooved Microstrip
patch antenna array. The characteristics of the antenna are
determined in terms of Return loss, VSWR, gain, radiation pattern
etc. The measured and simulated results presented are found to be in
good agreement.
Abstract: This paper presents Cost per Equivalent Wafer Out, which we find useful in wafer fab operational cost monitoring and controlling. It removes the loading and product mix effect in the cost variance analysis. The operation heads, therefore, could immediately focus on identifying areas for cost improvement. Without this, they would have to measure the impact of the loading variance and product mix variance between actual and budgeted prior to make any decision on cost improvement. Cost per Equivalent Wafer Out, thereby, increases efficiency in wafer fab operational cost monitoring and controlling.
Abstract: In this paper, computational fluid dynamics (CFD) is utilized to characterize a prototype biolistic delivery system, the biomedical device based on the contoured-shock-tube design (CST), with the aim at investigating shocks induced flow instabilities within the contoured shock tube. The shock/interface interactions, the growth of perturbation at an interface between two fluids of different density are interrogated. The key features of the gas dynamics and gas-particle interaction are discussed
Abstract: Aligned and random nanofibrous scaffolds of PVA/PCL/nHA were fabricated by electrospinning method. The composite nanofibrous scaffolds were subjected to detailed analysis. Morphological investigations revealed that the prepared nanofibers have uniform morphology and the average fiber diameters of aligned and random scaffolds were 135.5 and 290 nm, respectively. The obtained scaffolds have a porous structure with porosity of 88 and 76% for random and aligned nanofibers, respectively. Furthermore, FTIR analysis demonstrated that there were strong intramolecular interactions between the molecules of PVA/PCL/nHA. On the other hand, mechanical characterizations show that aligning the nanofibers, could significantly improve the rigidity of the resultant biocomposite nanofibrous scaffolds.
Abstract: The study explored varied types of human smiles and
extracted most of the key factors affecting the smiles. These key
factors then were converted into a set of control points which could
serve to satisfy the needs for creation of facial expression for 3D
animators and be further applied to the face simulation for robots in the
future. First, hundreds of human smile pictures were collected and
analyzed to identify the key factors for face expression. Then, the
factors were converted into a set of control points and sizing
parameters calculated proportionally. Finally, two different faces
were constructed for validating the parameters via the process of
simulating smiles of the same type as the original one.
Abstract: The Integrated Performance Modelling Environment
(IPME) is a powerful simulation engine for task simulation and
performance analysis. However, it has no high level cognition such
as memory and reasoning for complex simulation. This article
introduces a knowledge representation and reasoning scheme that can
accommodate uncertainty in simulations of military personnel with
IPME. This approach demonstrates how advanced reasoning models
that support similarity-based associative process, rule-based abstract
process, multiple reasoning methods and real-time interaction can be
integrated with conventional task network modelling to provide
greater functionality and flexibility when modelling operator
performance.
Abstract: Modeling and simulation of fixed bed three-phase
catalytic reactors are considered for wet air catalytic oxidation of
phenol to perform a comparative numerical analysis between tricklebed
and packed-bubble column reactors. The modeling involves
material balances both for the catalyst particle as well as for different
fluid phases. Catalyst deactivation is also considered in a transient
reactor model to investigate the effects of various parameters
including reactor temperature on catalyst deactivation. The
simulation results indicated that packed-bubble columns were
slightly superior in performance than trickle beds. It was also found
that reaction temperature was the most effective parameter in catalyst
deactivation.
Abstract: Prostate cancer is one of the most frequent cancers in men and is a major cause of mortality in the most of countries. In many diagnostic and treatment procedures for prostate disease accurate detection of prostate boundaries in transrectal ultrasound (TRUS) images is required. This is a challenging and difficult task due to weak prostate boundaries, speckle noise and the short range of gray levels. In this paper a novel method for automatic prostate segmentation in TRUS images is presented. This method involves preprocessing (edge preserving noise reduction and smoothing) and prostate segmentation. The speckle reduction has been achieved by using stick filter and top-hat transform has been implemented for smoothing. A feed forward neural network and local binary pattern together have been use to find a point inside prostate object. Finally the boundary of prostate is extracted by the inside point and an active contour algorithm. A numbers of experiments are conducted to validate this method and results showed that this new algorithm extracted the prostate boundary with MSE less than 4.6% relative to boundary provided manually by physicians.
Abstract: This paper presents a novel control strategy of a threephase
four-wire Unified Power Quality (UPQC) for an improvement
in power quality. The UPQC is realized by integration of series and
shunt active power filters (APFs) sharing a common dc bus capacitor.
The shunt APF is realized using a thee-phase, four leg voltage source
inverter (VSI) and the series APF is realized using a three-phase,
three leg VSI. A control technique based on unit vector template
technique (UTT) is used to get the reference signals for series APF,
while instantaneous sequence component theory (ISCT) is used for
the control of Shunt APF. The performance of the implemented
control algorithm is evaluated in terms of power-factor correction,
load balancing, neutral source current mitigation and mitigation of
voltage and current harmonics, voltage sag and swell in a three-phase
four-wire distribution system for different combination of linear and
non-linear loads. In this proposed control scheme of UPQC, the
current/voltage control is applied over the fundamental supply
currents/voltages instead of fast changing APFs currents/voltages,
there by reducing the computational delay and the required sensors.
MATLAB/Simulink based simulations are obtained, which support
the functionality of the UPQC. MATLAB/Simulink based
simulations are obtained, which support the functionality of the
UPQC.
Abstract: In seismic survey, the information regarding the
velocity of compression wave (Vp) as well as shear wave (Vs) are
very useful especially during the seismic interpretation. Previous
studies showed that both Vp and Vs determined by above methods
are totally different with respect to each other but offered good
approximation. In this study, both Vp and Vs of consolidated granite
rock were studied by using ultrasonic testing method and seismic
refraction method. In ultrasonic testing, two different condition of
rock are used which is dry and wet. The differences between Vp and
Vs getting by using ultrasonic testing and seismic refraction were
investigated and studied. The effect of water content in granite rock
towards the value of Vp and Vs during ultrasonic testing are also
measured. Within this work, the tolerance of the differences between
the velocity of seismic wave getting from ultrasonic testing and the
velocity of seismic wave getting from seismic refraction are also
measured and investigated.
Abstract: With the exponential progress of technological
development comes a strong sense that events are moving too quickly
for our schools and that teachers may be losing control of them in the
process. This paper examines the impact of e-learning and e-teaching
in universities, from both the student and teacher perspective. In
particular, it is shown that e-teachers should focus not only on the
technical capacities and functions of IT materials and activities, but
must attempt to more fully understand how their e-learners perceive
the learning environment. From the e-learner perspective, this paper
indicates that simply having IT tools available does not automatically
translate into all students becoming effective learners. More
evidence-based evaluative research is needed to allow e-learning and
e-teaching to reach full potential.
Abstract: As business environments are rapidly changing,
the manufacturing system must be reconfigured to adapt to
various customer needs. In order to cope with this challenge, it
is quintessential to test industrial control logic rapidly and
easily in the design time, and monitor operational behavior in
the run time of automated manufacturing system. Proposed
integrated model for virtual prototyping and operational
monitoring of industrial control logic is to improve limitations
of current ladder programming practices and general discrete
event simulation method. Each plant layout model using HMI
package and object-oriented control logic model is designed
independently and is executed simultaneously in integrated
manner to reflect design practices of automation system in the
design time. Control logic is designed and executed using UML
activity diagram without considering complicated control
behavior to deal with current trend of reconfigurable
manufacturing. After the physical installation, layout model of
virtual prototype constructed in the design time is reused for
operational monitoring of system behavior during run time.
Abstract: The peculiarities of the nanoscale structure-phase
states formed after electroexplosive carburizing and subsequent
electron-beam treatment of technically pure titanium surface in different regimes are established by methods of transmission electron
diffraction microscopy and physical mechanisms are discussed. Electroexplosive carburizing leads to surface layer formation
(40 m thickness) with increased (in 3.5 times) microhardness. It consists of β-titanium, graphite (monocrystals 100-150 nm,
polycrystals 5-10 nm, amorphous particles 3-5nm), TiC (5-10 nm), β-Ti02 (2-20nm). After electron-beam treatment additionally increasing the microhardness the surface layer consists of TiC.
Abstract: System-level design based on high-level abstractions
is becoming increasingly important in hardware and embedded
system design. This paper analyzes meta-design techniques oriented
at developing meta-programs and meta-models for well-understood
domains. Meta-design techniques include meta-programming and
meta-modeling. At the programming level of design process, metadesign
means developing generic components that are usable in a
wider context of application than original domain components. At the
modeling level, meta-design means developing design patterns that
describe general solutions to the common recurring design problems,
and meta-models that describe the relationship between different
types of design models and abstractions. The paper describes and
evaluates the implementation of meta-design in hardware design
domain using object-oriented and meta-programming techniques.
The presented ideas are illustrated with a case study.