Abstract: Liposomal magnetofection is a simple, highly efficient
technology for cell transfection, demonstrating better outcome than a
number of other common gene delivery methods. However,
aggregate complexes distribution over the cell surface is non-uniform
due to the gradient of the permanent magnetic field. The aim of this
study was to estimate the efficiency of liposomal magnetofection for
prostate carcinoma PC3 cell line using newly designed device,
“DynaFECTOR", ensuring magnetofection in a dynamic gradient
magnetic field. Liposomal magnetofection in a dynamic gradient
magnetic field demonstrated the highest transfection efficiency for
PC3 cells – it increased for 21% in comparison with liposomal
magnetofection and for 42% in comparison with lipofection alone.
The optimal incubation time under dynamic magnetic field for PC3
cell line was 5 minutes and the optimal rotation frequency of
magnets – 5 rpm. The new approach also revealed lower cytotoxic
effect to cells than liposomal magnetofection.
Abstract: Direct fermentation of 226 white rose tapioca stem to
ethanol by Fusarium oxysporum was studied in a batch reactor.
Fermentation of ethanol can be achieved by sequential pretreatment
using dilute acid and dilute alkali solutions using 100 mesh tapioca
stem particles. The quantitative effects of substrate concentration, pH
and temperature on ethanol concentration were optimized using a full
factorial central composite design experiment. The optimum process
conditions were then obtained using response surface methodology.
The quadratic model indicated that substrate concentration of 33g/l,
pH 5.52 and a temperature of 30.13oC were found to be optimum for
maximum ethanol concentration of 8.64g/l. The predicted optimum
process conditions obtained using response surface methodology was
verified through confirmatory experiments. Leudeking-piret model
was used to study the product formation kinetics for the production
of ethanol and the model parameters were evaluated using
experimental data.
Abstract: Gamboge disorder (GD) or fruit damage by the yellow sap is a major problem in mangosteen. Mangosteen plants varied in the level of GD, from very low or non GD to low, moderate and high GD. However it was difficult to differentiate between GD and non GD plants because evaluation of the disorder is strongly influenced by environment. In this study we investigated the usefulness of primer designed from bioinformatics related to cell wall strength, termed as MCWS, to predict GD. Plant materials used were 28 mangosteen plants selected based on percentage of GD categorized as high, moderate, low and very low or non GD. The result showed that the specific DNA fragments were absent in the high GD accessions. The MCWS marker suggests as a novel polymorphic marker for GD in mangosteen as well as a marker for detect variability in mangosteen as apomictic plant.
Abstract: Two novel hydrodesulfurization (HDS) catalysts:
FeN4/C and FeN2/C, were prepared using an impregnation-pyrolysis
method. The two materials were investigated as catalysts for
hydrodesulfurization (HDS) and hydrodearomitization (HDA) of
model compounds. The turnover frequency of the two FeN catalysts
is comparable to (FeN4/C) or even higher (FeN2/C) than that of
MoNi/Al2O3. The FeN4/C catalyst also exhibited catalytic activity
toward HDA.
Abstract: In CMOS integrated circuit design there is a trade-off between static power consumption and technology scaling. Recently, the power density has increased due to combination of higher clock speeds, greater functional integration, and smaller process geometries. As a result static power consumption is becoming more dominant. This is a challenge for the circuit designers. However, the designers do have a few methods which they can use to reduce this static power consumption. But all of these methods have some drawbacks. In order to achieve lower static power consumption, one has to sacrifice design area and circuit performance. In this paper, we propose a new method to reduce static power in the CMOS VLSI circuit using Variable Body Biasing technique without being penalized in area requirement and circuit performance.
Abstract: Determination of wellbore problems during a
production/injection process might be evaluated thorough
temperature log analysis. Other applications of this kind of log
analysis may also include evaluation of fluid distribution analysis
along the wellbore and identification of anomalies encountered
during production/injection process. While the accuracy of such
prediction is paramount, the common method of determination of a
wellbore temperature log includes use of steady-state energy balance
equations, which hardly describe the real conditions as observed in
typical oil and gas flowing wells during production operation; and
thus increase level of uncertainties. In this study, a practical method
has been proposed through development of a simplified semianalytical
model to apply for predicting temperature profile along the
wellbore. The developed model includes an overall heat transfer
coefficient accounting all modes of heat transferring mechanism,
which has been focused on the prediction of a temperature profile as
a function of depth for the injection/production wells. The model has
been validated with the results obtained from numerical simulation.
Abstract: Electrochemical-oxidation of Reactive Black-5 (RB- 5) was conducted for degradation using DSA type Ti/RuO2-SnO2- Sb2O5 electrode. In the study, for electro-oxidation, electrode was indigenously fabricated in laboratory using titanium as substrate. This substrate was coated using different metal oxides RuO2, Sb2O5 and SnO2 by thermal decomposition method. Laboratory scale batch reactor was used for degradation and decolorization studies at pH 2, 7 and 11. Current density (50mA/cm2) and distance between electrodes (8mm) were kept constant for all experiments. Under identical conditions, removal of color, COD and TOC at initial pH 2 was 99.40%, 55% and 37% respectively for initial concentration of 100 mg/L RB-5. Surface morphology and composition of the fabricated electrode coatings were characterized using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) respectively. Coating microstructure was analyzed by X-ray diffraction (XRD). Results of this study further revealed that almost 90% of oxidation occurred within 5-10 minutes.
Abstract: Purpose of this paper is two-folded. At first it explains
the major problems that are causing stagnation in brownfield
redevelopment. In addition, these problems given the context of the
present multi-actor built environment are becoming more complex to
observe. Therefore, this paper suggests also a prospective decisionmaking
approach that is the most appropriate to observe and react on
the given stagnation problems. Such an approach should be regarded
as prescriptive-interactive decision-making approach, a barely
established branch. This approach should offer models that have
prescriptive as well as an interactive component enabling them to
successfully cope with the multi-actor environment. Overall, this
paper provides up-to-date insight on the brownfield stagnation by
gradually introducing the nowadays major problems and offers a
prospective decision-making approach how these problems could be
tackled.
Abstract: Opportunistic network is a kind of Delay Tolerant Networks (DTN) where the nodes in this network come into contact with each other opportunistically and communicate wirelessly and, an end-to-end path between source and destination may have never existed, and disconnection and reconnection is common in the network. In such a network, because of the nature of opportunistic network, perhaps there is no a complete path from source to destination for most of the time and even if there is a path; the path can be very unstable and may change or break quickly. Therefore, routing is one of the main challenges in this environment and, in order to make communication possible in an opportunistic network, the intermediate nodes have to play important role in the opportunistic routing protocols. In this paper we proposed an Adaptive Fuzzy Routing in opportunistic network (AFRON). This protocol is using the simple parameters as input parameters to find the path to the destination node. Using Message Transmission Count, Message Size and Time To Live parameters as input fuzzy to increase delivery ratio and decrease the buffer consumption in the all nodes of network.
Abstract: The main aim of this paper is to develop and calibrate
an econometric model for modeling prices of long term electricity
futures contracts. The calibration of our model is performed on data
from EEX AG allowing us to capture the specific features of German
electricity market. The data sample contains several structural breaks
which have to be taken into account for modeling. We model the data
with an ARIMAX model which reveals high correlation between the
price of electricity futures contracts and prices of LT futures
contracts of fuels (namely coal, natural gas and crude oil). Besides
this, also a share price index of representative electricity companies
traded on Xetra, spread between 10Y and 1Y German bonds and
exchange rate between EUR and USD appeared to have significant
explanatory power over these futures contracts on EEX.
Abstract: Triglycerides and their derivatives are considered as viable alternatives for diesel fuels. Rice bran oil is used as diesel fuel. Highly viscous rice bran oil can be reduced by blending it with diesel fuel. The present research is aimed to investigate experimentally the performance, exhaust emission and combustion characteristics of a direct injection (DI) diesel engine, typically used in agricultural sector, over the entire load range when fuelled with rice bran oil and diesel fuel blends, RB10 (10% rice bran oil + 90% diesel fuel) to RB50. The performance, emission and combustion parameters of RB20 were found to be very close to neat diesel fuel (ND). The injector opening pressure (IOP) undoubtedly is of prime importance in diesel engine operation. Performance, emission and combustion characteristics with RB30 at enhanced IOPs are better than ND. Improved premixed heat release rate were noticed with RB30 when the IOP is enhanced.
Abstract: Design of Converter transformer insulation is a major
challenge. The insulation of these transformers is stressed by both
AC and DC voltages. Particle contamination is one of the major
problems in insulation structures, as they generate partial discharges
leading it to major failure of insulation. Similarly corona discharges
occur in transformer insulation. This partial discharge due to particle
movement / corona formation in insulation structure under different
voltage wave shapes, are different. In the present study, UHF
technique is adopted to understand the discharge activity and could
be realized that the characteristics of UHF signal generated under
low and high fields are different. In the case of corona generated
signal, the frequency content of the UHF sensor output lies in the
range 0.3-1.2 GHz and is not much varied except for its increase in
magnitude of discharge with the increase in applied voltage. It is
realized that the current signal injected due to partial
discharges/corona is about 4ns duration measured for first one half
cycle. Wavelet technique is adopted in the present study. It allows
one to identify the frequency content present in the signal at different
instant of time. The STD-MRA analysis helps one to identify the
frequency band in which the energy content of the UHF signal is
maximum.
Abstract: Modern building automation needs to deal with very
different types of demands, depending on the use of a building and the
persons acting in it. To meet the requirements of situation awareness
in modern building automation, scenario recognition becomes more
and more important in order to detect sequences of events and to react
to them properly. We present two concepts of scenario recognition
and their implementation, one based on predefined templates and the
other applying an unsupervised learning algorithm using statistical
methods. Implemented applications will be described and their advantages
and disadvantages will be outlined.
Abstract: The performances of a thermoacoustic travelling-wave
refrigerator are presented. Developed in the frame of the European
project called THATEA, it is designed for providing 600 W at a
temperature of 233 K with an efficiency of 40 % relative to the
Carnot efficiency. This paper presents the device and the results of
the first measurements. For a cooling power of 210 W, a coefficient
of performance relative to Carnot of 30 % is achieved when the
refrigerator is coupled with an existing standing-wave engine.
Abstract: Collected data must be organized to be utilized efficiently, and hierarchical classification of data is efficient approach to organize data. When data is classified to multiple categories or annotated with a set of labels, users request multi-labeled data by giving a set of labels. There are several interpretations of the data expressed by a set of labels. This paper discusses which data is expressed by a set of labels by introducing orders for sets of labels and shows that there are four types of orders, which are characterized by whether the labels of expressed data includes every label of the given set of labels within the range of the set. Desirable properties of the orders, data is also expressed by the higher set of labels and different sets of labels express different data, are discussed for the orders.
Abstract: In this work, statistical experimental design was
applied for the optimization of medium constituents for Gentamicin
production by Micromsonospora echinospora subs pallida (MTCC
708) in a batch reactor and the results are compared with the ANN
predicted values. By central composite design, 50 experiments are
carried out for five test variables: Starch, Soya bean meal, K2HPO4,
CaCO3 and FeSO4. The optimum condition was found to be: Starch
(8.9,g/L), Soya bean meal (3.3 g/L), K2HPO4 (0.8 g/L), CaCO3 (4
g/L) and FeSO4 (0.03 g/L). At these optimized conditions, the yield
of gentamicin was found to be 1020 mg/L. The R2 values were found
to be 1 for ANN training set, 0.9953 for ANN test set, and 0.9286 for
RSM.
Abstract: This paper presents an information retrieval model on
XML documents based on tree matching. Queries and documents are
represented by extended trees. An extended tree is built starting from
the original tree, with additional weighted virtual links between each
node and its indirect descendants allowing to directly reach each
descendant. Therefore only one level separates between each node
and its indirect descendants. This allows to compare the user query
and the document with flexibility and with respect to the structural
constraints of the query. The content of each node is very important to
decide weither a document element is relevant or not, thus the content
should be taken into account in the retrieval process. We separate
between the structure-based and the content-based retrieval processes.
The content-based score of each node is commonly based on the
well-known Tf × Idf criteria. In this paper, we compare between
this criteria and another one we call Tf × Ief. The comparison
is based on some experiments into a dataset provided by INEX1 to
show the effectiveness of our approach on one hand and those of
both weighting functions on the other.
Abstract: Use of the Internet and the World-Wide-Web
(WWW) has become widespread in recent years and mobile agent
technology has proliferated at an equally rapid rate. In this scenario
load balancing becomes important for P2P systems. Beside P2P
systems can be highly heterogeneous, i.e., they may consists of peers
that range from old desktops to powerful servers connected to
internet through high-bandwidth lines. There are various loads
balancing policies came into picture. Primitive one is Message
Passing Interface (MPI). Its wide availability and portability make it
an attractive choice; however the communication requirements are
sometimes inefficient when implementing the primitives provided by
MPI. In this scenario we use the concept of mobile agent because
Mobile agent (MA) based approach have the merits of high
flexibility, efficiency, low network traffic, less communication
latency as well as highly asynchronous. In this study we present
decentralized load balancing scheme using mobile agent technology
in which when a node is overloaded, task migrates to less utilized
nodes so as to share the workload. However, the decision of which
nodes receive migrating task is made in real-time by defining certain
load balancing policies. These policies are executed on PMADE (A
Platform for Mobile Agent Distribution and Execution) in
decentralized manner using JuxtaNet and various load balancing
metrics are discussed.
Abstract: The Taiwan government has invested approximately
21 billion NT dollars in the construction of bicycle paths since
bicycling has gained huge popularity as a healthy leisure and
recreational activity. This study focuses on the behavior of
recreational bicyclists in Danshuei and Bali, northern Taiwan. Data
were collected from a field investigation carried out along the
Danshuei bicycle path and Bali left-bank bicycle path. A total of 578
questionnaires were gathered for data analysis. Descriptive statistics
and Chi-Square tests were used to assess bicyclists- behaviors. The
frequency shows that, in these areas, Danshuei and Bali, most
bicyclists rented bicycles, rode the bicycle path in the afternoon for
about 2 hours. The used the bicycle path one time per week. For most,
it was the first time to ride these bicycle paths. There were significant
differences in distribution of bicycle ownership, time of day, duration
of ride, ride frequency, and whether riding occurred on weekdays or
weekends. Results indicated that most bicyclists in Danshuei and Bali
were infrequent users.
Abstract: Solar sunspot rotation, latitudinal bands are studied based on intelligent computation methods. A combination of image fusion method with together tree decomposition is used to obtain quantitative values about the latitudes of trajectories on sun surface that sunspots rotate around them. Daily solar images taken with SOlar and Heliospheric (SOHO) satellite are fused for each month separately .The result of fused image is decomposed with Quad Tree decomposition method in order to achieve the precise information about latitudes of sunspot trajectories. Such analysis is useful for gathering information about the regions on sun surface and coordinates in space that is more expose to solar geomagnetic storms, tremendous flares and hot plasma gases permeate interplanetary space and help human to serve their technical systems. Here sunspot images in September, November and October in 2001 are used for studying the magnetic behavior of sun.