Improved Technique of Non-viral Gene Delivery into Cancer Cells
Liposomal magnetofection is a simple, highly efficient
technology for cell transfection, demonstrating better outcome than a
number of other common gene delivery methods. However,
aggregate complexes distribution over the cell surface is non-uniform
due to the gradient of the permanent magnetic field. The aim of this
study was to estimate the efficiency of liposomal magnetofection for
prostate carcinoma PC3 cell line using newly designed device,
“DynaFECTOR", ensuring magnetofection in a dynamic gradient
magnetic field. Liposomal magnetofection in a dynamic gradient
magnetic field demonstrated the highest transfection efficiency for
PC3 cells – it increased for 21% in comparison with liposomal
magnetofection and for 42% in comparison with lipofection alone.
The optimal incubation time under dynamic magnetic field for PC3
cell line was 5 minutes and the optimal rotation frequency of
magnets – 5 rpm. The new approach also revealed lower cytotoxic
effect to cells than liposomal magnetofection.
[1] D. Luo & W.M Saltzman, "Enhancement of transfection by physical
concentration of DNA at the cell surface," Nat Biotechnol, vol. 18, pp.
893-589, 2000.
[2] C. Plank, M. Anton, C. Rudolph, J. Rosenecker, F. Krötz, "Enhancing
and targeting nucleic acid delivery by magnetic force," Expert. Opin.
Biol. Ther., vol. 3, pp. 745-758, 2003.
[3] C. Plank, U. Schillinger, F. Scherer, C. Bergemann, J.S. Rémy, F. Krötz,
M. Anton, J. Lausier, J. Rosenecker, "The magnetofection method: using
magnetic force to enhance gene delivery," Biol. Chem., vol. 384, pp.
737-747, 2003.
[4] S.W. Gersting, U. Schillinger, J. Lausier, P. Nicklaus, C. Rudolph, C.
Plank, D. Reinhardt, J. Rosenecker, "Gene delivery to respiratory
epithelial cells by magnetofection," J Gene Med., vol. 6, pp. 913-922,
2004.
[5] S. Huth, J. Lausier, S.W. Gersting, C. Rudolph, C. Plank, U. Welsch, J.
Rosenecker, "Insights into the mechanism of magnetofection using PEIbased
magnetofectins for gene transfer," J Gene Med., vol. 6, pp. 923-
936, 2004.
[6] S. W. Kamau, P. O. Hassa, B. Steitz, A. Petri-Fink, H. Hofmann, M.
Hofmann- Amtenbrink, B. von Rechenberg and M.O. Hottiger,
"Enhancement of the efficiency of non-viral gene delivery by
application of pulsed magnetic field," Nucleic Acids Research, vol. 34,
e40, 2006.
[7] S.C. McBain, U. Griesenbach, S. Xenariou, A. Keramane, C.D. Batich,
E.W.F.W, Alton, et al, ÔÇ×Magnetic nanoparticles as gene delivery agents:
enhanced transfection in the presence of oscillating magnet arrays",
Nanotechnology, vol. 19, no. 40, 2008.
[8] O. Mykhaylyk, Y.S. Antequera, D. Vlaskou, C. Plank, "Generation of
magnetic nonviral gene transfer agents and magnetofection in vitro,"
Nat. Protoc., vol. 2, pp. 2391-2411, 2007.
[9] O. Mykhaylyk, O. Zelphati, J. Rosenecker, C. Plank, "siRNA delivery
by magnetofection," Curr Opin Mol Ther., vol. 10, no. 5, pp. 493-505,
2008.
[10] O. Mykhaylyk, O. Zelphati, E. Hammerschmid, M. Anton, J.
Rosenecker, C. Plank, "Recent advances in magnetofection and its
potential to deliver siRNAs in vitro," Methods Mol Biol., vol. 487, pp.
111-146, 2009.
[11] O. Mykhaylyk, Y. Sánchez-Antequera, D. Vlaskou, E. Hammerschmid,
M. Anton, O. Zelphati, C. Plank, "Liposomal magnetofection," Methods
Mol Biol., vol. 605, pp. 487-525, 2010.
[12] C.H. Lee, E.Y. Kim, K. Jeon, J.C. Tae, K.S. Lee, Y.O. Kim, M.Y. Jeong,
C.W. Yun, D.K. Jeong, S.K. Cho, J.H. Kim, H.Y. Lee, K.Z. Riu, S.G.
Cho, S.P. Park, "Simple, efficient, and reproducible gene transfection of
mouse embryonic stem cells by magnetofection," Stem Cells Dev., vol.
17, no. 1, pp. 133-141, 2008.
[13] C. Fallini, G.J. Bassell, W. Rossoll, "High-efficiency transfection of
cultured primary motor neurons to study protein localization, trafficking,
and function," Mol Neurodegener., vol. 5, pp. 17, 2010.
[14] M. Pickard and D. Chari "Enhancement of magnetic nanoparticlemediated
gene transfer to astrocytes by magnetofection: effects of static
and oscillating fields," Nanomedicine (Lond), vol. 5, no. 2, 2010.
[15] C. Sapet, N. Laurent, A. de Chevigny, L. Le Gourrierec, E. Bertosio, O.
Zelphati, C. Béclin, High transfection efficiency of neural stem cells
with magnetofection," Biotechniques, vol. 50, no. 3, pp. 187-189, 2011.
[16] D. Ribble, N.B. Goldstein, D.A. Norris, and Y.G. Shellman, "A simple
technique for quantifying apoptosis in 96-well plates," BMC
Biotechnology, vol.5, no. 12, pp. 5-12, 2005.
[1] D. Luo & W.M Saltzman, "Enhancement of transfection by physical
concentration of DNA at the cell surface," Nat Biotechnol, vol. 18, pp.
893-589, 2000.
[2] C. Plank, M. Anton, C. Rudolph, J. Rosenecker, F. Krötz, "Enhancing
and targeting nucleic acid delivery by magnetic force," Expert. Opin.
Biol. Ther., vol. 3, pp. 745-758, 2003.
[3] C. Plank, U. Schillinger, F. Scherer, C. Bergemann, J.S. Rémy, F. Krötz,
M. Anton, J. Lausier, J. Rosenecker, "The magnetofection method: using
magnetic force to enhance gene delivery," Biol. Chem., vol. 384, pp.
737-747, 2003.
[4] S.W. Gersting, U. Schillinger, J. Lausier, P. Nicklaus, C. Rudolph, C.
Plank, D. Reinhardt, J. Rosenecker, "Gene delivery to respiratory
epithelial cells by magnetofection," J Gene Med., vol. 6, pp. 913-922,
2004.
[5] S. Huth, J. Lausier, S.W. Gersting, C. Rudolph, C. Plank, U. Welsch, J.
Rosenecker, "Insights into the mechanism of magnetofection using PEIbased
magnetofectins for gene transfer," J Gene Med., vol. 6, pp. 923-
936, 2004.
[6] S. W. Kamau, P. O. Hassa, B. Steitz, A. Petri-Fink, H. Hofmann, M.
Hofmann- Amtenbrink, B. von Rechenberg and M.O. Hottiger,
"Enhancement of the efficiency of non-viral gene delivery by
application of pulsed magnetic field," Nucleic Acids Research, vol. 34,
e40, 2006.
[7] S.C. McBain, U. Griesenbach, S. Xenariou, A. Keramane, C.D. Batich,
E.W.F.W, Alton, et al, ÔÇ×Magnetic nanoparticles as gene delivery agents:
enhanced transfection in the presence of oscillating magnet arrays",
Nanotechnology, vol. 19, no. 40, 2008.
[8] O. Mykhaylyk, Y.S. Antequera, D. Vlaskou, C. Plank, "Generation of
magnetic nonviral gene transfer agents and magnetofection in vitro,"
Nat. Protoc., vol. 2, pp. 2391-2411, 2007.
[9] O. Mykhaylyk, O. Zelphati, J. Rosenecker, C. Plank, "siRNA delivery
by magnetofection," Curr Opin Mol Ther., vol. 10, no. 5, pp. 493-505,
2008.
[10] O. Mykhaylyk, O. Zelphati, E. Hammerschmid, M. Anton, J.
Rosenecker, C. Plank, "Recent advances in magnetofection and its
potential to deliver siRNAs in vitro," Methods Mol Biol., vol. 487, pp.
111-146, 2009.
[11] O. Mykhaylyk, Y. Sánchez-Antequera, D. Vlaskou, E. Hammerschmid,
M. Anton, O. Zelphati, C. Plank, "Liposomal magnetofection," Methods
Mol Biol., vol. 605, pp. 487-525, 2010.
[12] C.H. Lee, E.Y. Kim, K. Jeon, J.C. Tae, K.S. Lee, Y.O. Kim, M.Y. Jeong,
C.W. Yun, D.K. Jeong, S.K. Cho, J.H. Kim, H.Y. Lee, K.Z. Riu, S.G.
Cho, S.P. Park, "Simple, efficient, and reproducible gene transfection of
mouse embryonic stem cells by magnetofection," Stem Cells Dev., vol.
17, no. 1, pp. 133-141, 2008.
[13] C. Fallini, G.J. Bassell, W. Rossoll, "High-efficiency transfection of
cultured primary motor neurons to study protein localization, trafficking,
and function," Mol Neurodegener., vol. 5, pp. 17, 2010.
[14] M. Pickard and D. Chari "Enhancement of magnetic nanoparticlemediated
gene transfer to astrocytes by magnetofection: effects of static
and oscillating fields," Nanomedicine (Lond), vol. 5, no. 2, 2010.
[15] C. Sapet, N. Laurent, A. de Chevigny, L. Le Gourrierec, E. Bertosio, O.
Zelphati, C. Béclin, High transfection efficiency of neural stem cells
with magnetofection," Biotechniques, vol. 50, no. 3, pp. 187-189, 2011.
[16] D. Ribble, N.B. Goldstein, D.A. Norris, and Y.G. Shellman, "A simple
technique for quantifying apoptosis in 96-well plates," BMC
Biotechnology, vol.5, no. 12, pp. 5-12, 2005.
@article{"International Journal of Biological, Life and Agricultural Sciences:57380", author = "D. Vainauska and S. Kozireva and A. Karpovs and M. Chistyakovs and M. Baryshev", title = "Improved Technique of Non-viral Gene Delivery into Cancer Cells", abstract = "Liposomal magnetofection is a simple, highly efficient
technology for cell transfection, demonstrating better outcome than a
number of other common gene delivery methods. However,
aggregate complexes distribution over the cell surface is non-uniform
due to the gradient of the permanent magnetic field. The aim of this
study was to estimate the efficiency of liposomal magnetofection for
prostate carcinoma PC3 cell line using newly designed device,
“DynaFECTOR", ensuring magnetofection in a dynamic gradient
magnetic field. Liposomal magnetofection in a dynamic gradient
magnetic field demonstrated the highest transfection efficiency for
PC3 cells – it increased for 21% in comparison with liposomal
magnetofection and for 42% in comparison with lipofection alone.
The optimal incubation time under dynamic magnetic field for PC3
cell line was 5 minutes and the optimal rotation frequency of
magnets – 5 rpm. The new approach also revealed lower cytotoxic
effect to cells than liposomal magnetofection.", keywords = "Dynamic gradient magnetic field, gene delivery,
liposomal magnetofection, prostate cancer cell line", volume = "5", number = "10", pages = "608-5", }
