Abstract: The process parameters, temperature, pH and
substrate concentration, were optimized for the production of
gentamicin using Micromonouspora echinospora. Experiments were
carried out according to central composite design in response surface
method. The optimum conditions for the maximum production of
gentamicin were found to be: temperature – 31.7oC, pH – 6.8 and
substrate concentration – 3%. At these optimized conditions the
production of gentamicin was found to be – 1040 mg/L. The R2 value
of 0.9465 indicates a good fitness of the model.
Abstract: In this work, statistical experimental design was
applied for the optimization of medium constituents for Gentamicin
production by Micromsonospora echinospora subs pallida (MTCC
708) in a batch reactor and the results are compared with the ANN
predicted values. By central composite design, 50 experiments are
carried out for five test variables: Starch, Soya bean meal, K2HPO4,
CaCO3 and FeSO4. The optimum condition was found to be: Starch
(8.9,g/L), Soya bean meal (3.3 g/L), K2HPO4 (0.8 g/L), CaCO3 (4
g/L) and FeSO4 (0.03 g/L). At these optimized conditions, the yield
of gentamicin was found to be 1020 mg/L. The R2 values were found
to be 1 for ANN training set, 0.9953 for ANN test set, and 0.9286 for
RSM.