Abstract: The cDNA encoding the 326 amino acids of a Class I
basic chitinase gene from Leucaena leucocephala de Wit (KB3,
Genbank accession: AAM49597) was cloned under the control of
CaMV35S promoter in pCAMBIA 1300 and transferred to
Koshihikari. Calli of Koshihikari rice was transformed with
agrobacterium with this construct expressing the chitinase and β-
glucouronidase (GUS). The frequencies of calli 90 % has been
obtained from rice seedlings cultured on NB medium. The high
regeneration frequencies, 74% was obtained from calli cultured on
regeneration medium containing 4 mg/l BAP, and 7 g/l phytagel at
25°C. Various factors were studied in order to establish a procedure
for the transformation of Koshihikari Agrobacterium tumefaciens.
Supplementation of 50 mM acetosyringone to the medium during
coculivation was important to enhance the frequency to transient
transformation. The 4 week-old scutellum-derived calli were
excellent starting materials. Selection medium based on NB medium
supplement with 40 mg/l hygromycin and 400 mg/l cefotaxime were
an optimized medium for selection of transformed rice calli. The
percentage of transformation 70 was obtained. Recombinant calli and
regenerated rice plants were checked the expression of chitinase and
gus by PCR, northern blot gel, southern blot gel, and gus assay.
Chitinase and gus were expressed in all parts of recombinant rice.
The rice line expressing the KB3 chiitnase was more resistant to the
blast fungus Fusarium monoliforme than control line.
Abstract: The avian phytohaemagglutinin skin test is being
proved as an in vivo system for the evaluation an avian in vivo T cell
mitogenicity. The test system was one week old Gallus domesticus
broiler Chickens. Five replicates were done for each of the whole,
1:10 dilutions of each of 0.05 IU tuberculin, tetanus immunoglobulin
and DPT vaccine as test materials. The evaluation parameters were
the skin indurations and lymphoblast percentages in bone marrow
lymphocytes.
Tuberculin indurations were 2.06 and 1.26mm for 0.05 IU
respectively while lymphoblast percent were 0.234 and 0.1
accordingly.
The skin indurations of 135mg/ml and 1.35mg/ml tetanus
immunoglobulin were 4.86 and 3.96mm while lymphoblast
percentages were 0.3 and 0.14 respectively.
The whole DPT and 1:10 concentration were with 4.5 and 3.2mm
while their lymphoblast percentages were 0.28 and 0.12 accordingly.
Thus the mitogenicity of the test materials was of dependant type.
Abstract: The objective of this research is to develop the
performance indicators (PIs) in operational level for the Pre-hospital Emergency Medical Service (EMS) system employing in Thailand. This research started with ascertaining the current pre-hospital care
system. The team analyzed the strategies of Narerthorn, a government unit under the ministry of public health, and the existing PIs of the pre-hospital care. Afterwards, the current National Strategic Plan of EMS development (2008-2012) of the Emergency
Medical Institute of Thailand (EMIT) was considered using strategic
analysis to developed Strategy Map (SM) and identified the Success
Factors (SFs). The analysis results from strategy map and SFs were used to develop the Performance Indicators (PIs). To verify the set of
PIs, the team has interviewed with the relevant practitioners for the possibilities to implement the PIs. To this paper, it was to ascertain
that all the developed PIs support the objectives of the strategic plan. Nevertheless, the results showed that the operational level PIs suited
only with the first dimension of National Strategic Plan
(infrastructure and information technology development). Besides,
the SF was the infrastructure development (to contribute the EMS system to people throughout with standard and efficiency both in normally and disaster conditions). Finally, twenty-nine indicators
were developed from the analysis results of SM and SFs.
Abstract: In this paper, transversal vibration of buried pipelines
during loading induced by underground explosions is analyzed. The
pipeline is modeled as an infinite beam on an elastic foundation, so
that soil-structure interaction is considered by means of transverse
linear springs along the pipeline. The pipeline behavior is assumed to
be ideal elasto-plastic which an ultimate strain value limits the plastic
behavior. The blast loading is considered as a point load, considering
the affected length at some point of the pipeline, in which the
magnitude decreases exponentially with time. A closed-form solution
for the quasi-static problem is carried out for both elastic and elasticperfect
plastic behaviors of pipe materials. At the end, a comparative
study on steel and polyethylene pipes with different sizes buried in
various soil conditions, affected by a predefined underground
explosion is conducted, in which effect of each parameter is
discussed.
Abstract: A conventional binding method for low power in a
high-level synthesis mainly focuses on finding an optimal binding for
an assumed input data, and obtains only one binding table. In this
paper, we show that a binding method which uses multiple binding
tables gets better solution compared with the conventional methods
which use a single binding table, and propose a dynamic bus binding
scheme for low power using multiple binding tables. The proposed
method finds multiple binding tables for the proper partitions of an
input data, and switches binding tables dynamically to produce the
minimum total switching activity. Experimental result shows that the
proposed method obtains a binding solution having 12.6-28.9%
smaller total switching activity compared with the conventional
methods.
Abstract: Blood pulse is an important human physiological signal commonly used for the understanding of the individual physical health. Current methods of non-invasive blood pulse sensing require direct contact or access to the human skin. As such, the performances of these devices tend to vary with time and are subjective to human body fluids (e.g. blood, perspiration and skin-oil) and environmental contaminants (e.g. mud, water, etc). This paper proposes a simulation model for the novel method of non-invasive acquisition of blood pulse using the disturbance created by blood flowing through a localized magnetic field. The simulation model geometry represents a blood vessel, a permanent magnet, a magnetic sensor, surrounding tissues and air in 2-dimensional. In this model, the velocity and pressure fields in the blood stream are described based on Navier-Stroke equations and the walls of the blood vessel are assumed to have no-slip condition. The blood assumes a parabolic profile considering a laminar flow for blood in major artery near the skin. And the inlet velocity follows a sinusoidal equation. This will allow the computational software to compute the interactions between the magnetic vector potential generated by the permanent magnet and the magnetic nanoparticles in the blood. These interactions are simulated based on Maxwell equations at the location where the magnetic sensor is placed. The simulated magnetic field at the sensor location is found to assume similar sinusoidal waveform characteristics as the inlet velocity of the blood. The amplitude of the simulated waveforms at the sensor location are compared with physical measurements on human subjects and found to be highly correlated.
Abstract: Electrospinning is a broadly used technology to obtain
polymeric nanofibers ranging from several micrometers down to
several hundred nanometers for a wide range of applications. It offers
unique capabilities to produce nanofibers with controllable porous
structure. With smaller pores and higher surface area than regular
fibers, electrospun fibers have been successfully applied in various
fields, such as, nanocatalysis, tissue engineering scaffolds, protective
clothing, filtration, biomedical, pharmaceutical, optical electronics,
healthcare, biotechnology, defense and security, and environmental
engineering. In this study, polyurethane nanofibers were obtained
under different electrospinning parameters. Fiber morphology and
diameter distribution were investigated in order to understand them
as a function of process parameters.
Abstract: This paper reports a case study on how a conceptual
and analytical thinking approach was used in Art and Design Department at Multimedia University (Malaysia) in addressing the
issues of one nation and its impact in the society through artworks. The art project was designed for students to increase the know-how
and develop creative thinking in design and communication. Goals of the design project were: (1) to develop creative thinking in design
and communication, (2) to increase student understanding on the
process of problem solving for design work, and (3) to use design
elements and principles to generate interest, attention and emotional responses. An exhibition entitled "One Nation" was showcased to
local and international viewers consisting of the general public, professionals, academics, artists and students. Findings indicate that the project supported several visual art standards, as well as
generated awareness in the society. This project may be of interest to
current and future art educators and others interested in the potential
of utilizing global issues as content for art, community and environment studies for the purpose of educational art.
Abstract: Software Development Risks Identification (SDRI),
using Fault Tree Analysis (FTA), is a proposed technique to identify
not only the risk factors but also the causes of the appearance of the
risk factors in software development life cycle. The method is based
on analyzing the probable causes of software development failures
before they become problems and adversely affect a project. It uses
Fault tree analysis (FTA) to determine the probability of a particular
system level failures that are defined by A Taxonomy for Sources of
Software Development Risk to deduce failure analysis in which an
undesired state of a system by using Boolean logic to combine a
series of lower-level events. The major purpose of this paper is to use
the probabilistic calculations of Fault Tree Analysis approach to
determine all possible causes that lead to software development risk
occurrence
Abstract: Software estimation accuracy is among the greatest
challenges for software developers. This study aimed at building and
evaluating a neuro-fuzzy model to estimate software projects
development time. The forty-one modules developed from ten
programs were used as dataset. Our proposed approach is compared
with fuzzy logic and neural network model and Results show that the
value of MMRE (Mean of Magnitude of Relative Error) applying
neuro-fuzzy was substantially lower than MMRE applying fuzzy
logic and neural network.
Abstract: Conventionally the selection of parameters depends
intensely on the operator-s experience or conservative technological
data provided by the EDM equipment manufacturers that assign
inconsistent machining performance. The parameter settings given by
the manufacturers are only relevant with common steel grades. A
single parameter change influences the process in a complex way.
Hence, the present research proposes artificial neural network (ANN)
models for the prediction of surface roughness on first commenced
Ti-15-3 alloy in electrical discharge machining (EDM) process. The
proposed models use peak current, pulse on time, pulse off time and
servo voltage as input parameters. Multilayer perceptron (MLP) with
three hidden layer feedforward networks are applied. An assessment
is carried out with the models of distinct hidden layer. Training of the
models is performed with data from an extensive series of
experiments utilizing copper electrode as positive polarity. The
predictions based on the above developed models have been verified
with another set of experiments and are found to be in good
agreement with the experimental results. Beside this they can be
exercised as precious tools for the process planning for EDM.
Abstract: Analytical solution of the first-order and third-order
shear deformation theories are developed to study the free vibration
behavior of simply supported functionally graded plates. The
material properties of plate are assumed to be graded in the thickness
direction as a power law distribution of volume fraction of the
constituents. The governing equations of functionally graded plates
are established by applying the Hamilton's principle and are solved
by using the Navier solution method. The influence of side-tothickness
ratio and constituent of volume fraction on the natural
frequencies are studied. The results are validated with the known
data in the literature.
Abstract: In this paper, we proposed a new routing protocol for
Unmanned Aerial Vehicles (UAVs) that equipped with directional
antenna. We named this protocol Directional Optimized Link State
Routing Protocol (DOLSR). This protocol is based on the well
known protocol that is called Optimized Link State Routing Protocol
(OLSR). We focused in our protocol on the multipoint relay (MPR)
concept which is the most important feature of this protocol. We
developed a heuristic that allows DOLSR protocol to minimize
the number of the multipoint relays. With this new protocol the
number of overhead packets will be reduced and the End-to-End
delay of the network will also be minimized. We showed through
simulation that our protocol outperformed Optimized Link State
Routing Protocol, Dynamic Source Routing (DSR) protocol and Ad-
Hoc On demand Distance Vector (AODV) routing protocol in
reducing the End-to-End delay and enhancing the overall
throughput. Our evaluation of the previous protocols was based
on the OPNET network simulation tool.
Abstract: Heterogeneity of solid waste characteristics as well as the complex processes taking place within the landfill ecosystem motivated the implementation of soft computing methodologies such as artificial neural networks (ANN), fuzzy logic (FL), and their combination. The present work uses a hybrid ANN-FL model that employs knowledge-based FL to describe the process qualitatively and implements the learning algorithm of ANN to optimize model parameters. The model was developed to simulate and predict the landfill gas production at a given time based on operational parameters. The experimental data used were compiled from lab-scale experiment that involved various operating scenarios. The developed model was validated and statistically analyzed using F-test, linear regression between actual and predicted data, and mean squared error measures. Overall, the simulated landfill gas production rates demonstrated reasonable agreement with actual data. The discussion focused on the effect of the size of training datasets and number of training epochs.
Abstract: The aim of the present study is to analyze the generation of osteoporotic vertebral bone induced by lack of calcium during growth period and analyze its effects for disc degeneration, based on biomechanical and histomorphometrical study. Mechanical and histomorphological characteristics of lumbar vertebral bones and discs of rats with calcium free diet (CFD) were detected and tracked by using high resolution in-vivo micro-computed tomography (in-vivo micro-CT), finite element (FE) and histological analysis. Twenty female Sprague-Dawley rats (6 weeks old, approximate weight 170g) were randomly divided into two groups (CFD group: 10, NOR group: 10). The CFD group was maintained on a refmed calcium-controlled semisynthetic diet without added calcium, to induce osteoporosis. All lumbar (L 1-L6) were scanned by using in vivo micro-CT with 35i.un resolution at 0, 4, 8 weeks to track the effects of CFD on the generation of osteoporosis. The fmdings of the present study indicated that calcium insufficiency was the main factor in the generation of osteoporosis and it induced lumbar vertebral disc degeneration. This study is a valuable experiment to firstly evaluate osteoporotic vertebral bone and disc degeneration induced by lack of calcium during growth period from a biomechanical and histomorphometrical point of view.
Abstract: research goal was to determine the expression levels cDNA of brain embrio at gestation days 10 (GD-10). The Electroforesis DNA results showed that GAPDH, Fibronectin1, Ncam1, Tenascin, Vimentin, Neurofilament heavy, Neurofilament medium and Neurofilament low were 447 bp, 462 bp, 293 bp. 416 bp, 327 bp, 301 bp, 398 bp and 289 bp. Result of real-time RT-PCR on brain Embryo at gestation days 10 showed that the expression of copy gen Fibronectin 36 copies, Ncam 21,708 copies; Tenascin 24,505 copies; Vimentin 538,554 copies; Neurofilament heavy 2,419 copies; Neurofilament medium 92,928 copies; Neurofilament low 125,809 copies. Vimentin expressed gene copies is very high compared with other gene copies. This condition are caused by Vimentin, that contribute to proliferate of brain development. The vimentin role to cell proliferation of brain.
Abstract: In order to assess optical fiber reliability in different environmental and stress conditions series of testing are performed simulating overlapping of chemical and mechanical controlled varying factors. Each series of testing may be compared using statistical processing: i.e. Weibull plots. Due to the numerous data to treat, a software application has appeared useful to interpret selected series of experiments in function of envisaged factors. The current paper presents a software application used in the storage, modelling and interpretation of experimental data gathered from optical fibre testing. The present paper strictly deals with the software part of the project (regarding the modelling, storage and processing of user supplied data).
Abstract: Contamination of heavy metals in tin tailings has
caused an interest in the scientific approach of their remediation. One
of the approaches is through phytoremediation, which is using tree
species to extract the heavy metals from the contaminated soils. Tin
tailings comprise of slime and sand tailings. This paper reports only
on the finding of the four timber species namely Acacia mangium,
Hopea odorata, Intsia palembanica and Swietenia macrophylla on
the removal of cadmium (Cd) and lead (Pb) from the slime tailings.
The methods employed for sampling and soil analysis are established
methods. Six trees of each species were randomly selected from a
0.25 ha plot for extraction and determination of their heavy metals.
The soil samples were systematically collected according to 5 x 5 m
grid from each plot. Results showed that the concentration of heavy
metals in soils and trees varied according to species. Higher
concentration of heavy metals was found in the stem than the
primary roots of all the species. A. Mangium accumulated the highest
total amount of Pb per hectare basis.
Abstract: Nothing that an effective cure for infertility happens
when we can find a unique solution, a great deal of study has been
done in this field and this is a hot research subject for to days study.
So we could analyze the men-s seaman and find out about fertility
and infertility and from this find a true cure for this, since this will be
a non invasive and low risk procedure, it will be greatly welcomed.
In this research, the procedure has been based on few Algorithms
enhancement and segmentation of images which has been done on
the images taken from microscope in different fertility institution and
have obtained a suitable result from the computer images which in
turn help us to distinguish these sperms from fluids and its
surroundings.
Abstract: In our current political climate of assessment and
accountability initiatives we are failing to prepare our children for a
participatory role in the creative economy. The field of education is
increasingly falling prey to didactic methodologies which train a
nation of competent test takers, foregoing the opportunity to educate
students to find problems and develop multiple solutions. No where is
this more evident than in the area of art education. Due to a myriad of
issues including budgetary shortfalls, time constraints and a general
misconception that anyone who enjoys the arts is capable of teaching
the arts, our students are not developing the skills they require to
become fully literate in critical thinking and creative processing.
Although art integrated curriculum is increasingly being viewed as a
reform strategy for motivating students by offering alternative
presentation of concepts and representation of knowledge acquisition,
misinformed administrators are often excluding the art teacher from
the integration equation. The paper to follow addresses the problem
of the need for divergent thinking and conceptualization in our
schools. Furthermore, this paper explores the role of education, and
specifically, art education in the development of a creatively literate
citizenry.