Abstract: In vitro storage and processing of animal semen represents a risk factor to spermatozoa vitality, potentially leading to reduced fertility. A variety of substances isolated from natural sources may exhibit protective or antioxidant properties on the spermatozoon, thus extending the lifespan of stored ejaculates. This study compared the ability of different concentrations of the Salvia officinalis extract on the motility, mitochondrial activity, viability and reactive oxygen species (ROS) production by bovine spermatozoa during different time periods (0, 2, 6 and 24 h) of in vitro culture. Spermatozoa motility was assessed using the Computer-assisted sperm analysis (CASA) system. Cell viability was examined using the metabolic activity MTT assay, the eosin-nigrosin staining technique was used to evaluate the sperm viability and ROS generation was quantified using luminometry. The CASA analysis revealed that the motility in the experimental groups supplemented with 0.5-2 µg/mL Salvia extract was significantly lower in comparison with the control (P
Abstract: The conventional assessment of human semen is a
highly subjective assessment, with considerable intra- and interlaboratory
variability. Computer-Assisted Sperm Analysis (CASA)
systems provide a rapid and automated assessment of the sperm
characteristics, together with improved standardization and quality
control. However, the outcome of CASA systems is sensitive to the
method of experimentation. While conventional CASA systems use
digital microscopes with phase-contrast accessories, producing
higher contrast images, we have used raw semen samples (no
staining materials) and a regular light microscope, with a digital
camera directly attached to its eyepiece, to insure cost benefits and
simple assembling of the system. However, since the accurate finding
of sperms in the semen image is the first step in the examination and
analysis of the semen, any error in this step can affect the outcome of
the analysis. This article introduces and explains an algorithm for
finding sperms in low contrast images: First, an image enhancement
algorithm is applied to remove extra particles from the image. Then,
the foreground particles (including sperms and round cells) are
segmented form the background. Finally, based on certain features
and criteria, sperms are separated from other cells.
Abstract: Nothing that an effective cure for infertility happens
when we can find a unique solution, a great deal of study has been
done in this field and this is a hot research subject for to days study.
So we could analyze the men-s seaman and find out about fertility
and infertility and from this find a true cure for this, since this will be
a non invasive and low risk procedure, it will be greatly welcomed.
In this research, the procedure has been based on few Algorithms
enhancement and segmentation of images which has been done on
the images taken from microscope in different fertility institution and
have obtained a suitable result from the computer images which in
turn help us to distinguish these sperms from fluids and its
surroundings.