Abstract: The conventional assessment of human semen is a
highly subjective assessment, with considerable intra- and interlaboratory
variability. Computer-Assisted Sperm Analysis (CASA)
systems provide a rapid and automated assessment of the sperm
characteristics, together with improved standardization and quality
control. However, the outcome of CASA systems is sensitive to the
method of experimentation. While conventional CASA systems use
digital microscopes with phase-contrast accessories, producing
higher contrast images, we have used raw semen samples (no
staining materials) and a regular light microscope, with a digital
camera directly attached to its eyepiece, to insure cost benefits and
simple assembling of the system. However, since the accurate finding
of sperms in the semen image is the first step in the examination and
analysis of the semen, any error in this step can affect the outcome of
the analysis. This article introduces and explains an algorithm for
finding sperms in low contrast images: First, an image enhancement
algorithm is applied to remove extra particles from the image. Then,
the foreground particles (including sperms and round cells) are
segmented form the background. Finally, based on certain features
and criteria, sperms are separated from other cells.