Abstract: MicroRNAs (miRNAs) are small, non-coding and
regulatory RNAs about 20 to 24 nucleotides long. Their conserved
nature among the various organisms makes them a good source of
new miRNAs discovery by comparative genomics approach. The
study resulted in 21 miRNAs of 20 pre-miRNAs belonging to 16
families (miR156, 157, 158, 164, 165, 168, 169, 172, 319, 390, 393,
394, 395, 400, 472 and 861) in evergreen spruce tree (Picea). The
miRNA families; miR 157, 158, 164, 165, 168, 169, 319, 390, 393,
394, 400, 472 and 861 are reported for the first time in the Picea. All
20 miRNA precursors form stable minimum free energy stem-loop
structure as their orthologues form in Arabidopsis and the mature
miRNA reside in the stem portion of the stem loop structure. Sixteen
(16) miRNAs are from Picea glauca and five (5) belong to Picea
sitchensis. Their targets consist of transcription factors, growth
related, stressed related and hypothetical proteins.
Abstract: The objective of this study is to evaluate the
occurrence of fungi in aerobic and anoxic activated sludge from
membrane bioreactors (MBRs). Thirty-six samples of both aerobic
and anoxic activated sludge were taken from 2 MBR treating
domestic wastewater. Over a period of eight months 2 samples from
each plant were taken per month. The samples were prepared for
count and definition of fungi. The obtained data show that, sixty
species belonging to 27 genera were collected from activated sludge
samples under aerobic and anoxic conditions. Regarding to the fungi
definition, under aerobic condition the Geotrichum was found at
(8.8%) followed by Penicillium (75.0%), Yeasts (65.7%) and
Trichoderma (55.5%), while Yeasts (77.1%) Geotrichum
candidumand Penicillium (61.1%) species were the most prevalent in
anoxic activated sludge. The results indicate that activated sludge is
habitat for growth and sporulation of different groups of fungi, both
saprophytic and pathogenic.
Abstract: As part of national epidemiological survey on bovine
viral diarrhea virus (BVDV), a total of 274 dejecta samples were
collected from 14 cattle farms in 8 areas of Xinjiang Uygur
Autonomous Region in northwestern China. Total RNA was extracted
from each sample, and 5--untranslated region (UTR) of BVDV
genome was amplified by using two-step reverse
transcriptase-polymerase chain reaction (RT-PCR). The PCR products
were subsequently sequenced to study the genetic variations of BVDV
in these areas. Among the 274 samples, 33 samples were found
virus-positive. According to sequence analysis of the PCR products,
the 33 samples could be arranged into 16 groups. All the sequences,
however, were highly conserved with BVDV Osloss strains. The virus
possessed theses sequences belonged to BVDV-1b subtype by
phylogenetic analysis. Based on these data, we established a typing
tree for BVDV in these areas. Our results suggested that BVDV-1b
was a predominant subgenotype in northwestern China and no
correlation between the genetic and geographical distances could be
observed above the farm level.
Abstract: It is important problems to increase the detection rates
and reduce false positive rates in Intrusion Detection System (IDS).
Although preventative techniques such as access control and
authentication attempt to prevent intruders, these can fail, and as a
second line of defence, intrusion detection has been introduced. Rare
events are events that occur very infrequently, detection of rare
events is a common problem in many domains. In this paper we
propose an intrusion detection method that combines Rough set and
Fuzzy Clustering. Rough set has to decrease the amount of data and
get rid of redundancy. Fuzzy c-means clustering allow objects to
belong to several clusters simultaneously, with different degrees of
membership. Our approach allows us to recognize not only known
attacks but also to detect suspicious activity that may be the result of
a new, unknown attack. The experimental results on Knowledge
Discovery and Data Mining-(KDDCup 1999) Dataset show that the
method is efficient and practical for intrusion detection systems.
Abstract: In many data mining applications, it is a priori known
that the target function should satisfy certain constraints imposed
by, for example, economic theory or a human-decision maker. In this
paper we consider partially monotone prediction problems, where the
target variable depends monotonically on some of the input variables
but not on all. We propose a novel method to construct prediction
models, where monotone dependences with respect to some of
the input variables are preserved by virtue of construction. Our
method belongs to the class of mixture models. The basic idea is to
convolute monotone neural networks with weight (kernel) functions
to make predictions. By using simulation and real case studies,
we demonstrate the application of our method. To obtain sound
assessment for the performance of our approach, we use standard
neural networks with weight decay and partially monotone linear
models as benchmark methods for comparison. The results show that
our approach outperforms partially monotone linear models in terms
of accuracy. Furthermore, the incorporation of partial monotonicity
constraints not only leads to models that are in accordance with the
decision maker's expertise, but also reduces considerably the model
variance in comparison to standard neural networks with weight
decay.
Abstract: Modern applications realized onto FPGAs exhibit high connectivity demands. Throughout this paper we study the routing constraints of Virtex devices and we propose a systematic methodology for designing a novel general-purpose interconnection network targeting to reconfigurable architectures. This network consists of multiple segment wires and SB patterns, appropriately selected and assigned across the device. The goal of our proposed methodology is to maximize the hardware utilization of fabricated routing resources. The derived interconnection scheme is integrated on a Virtex style FPGA. This device is characterized both for its high-performance, as well as for its low-energy requirements. Due to this, the design criterion that guides our architecture selections was the minimal Energy×Delay Product (EDP). The methodology is fully-supported by three new software tools, which belong to MEANDER Design Framework. Using a typical set of MCNC benchmarks, extensive comparison study in terms of several critical parameters proves the effectiveness of the derived interconnection network. More specifically, we achieve average Energy×Delay Product reduction by 63%, performance increase by 26%, reduction in leakage power by 21%, reduction in total energy consumption by 11%, at the expense of increase of channel width by 20%.
Abstract: Tofurther advance research on immune-related genes
from T. molitor, we constructed acDNA library and analyzed
expressed sequence taq (EST) sequences from 1,056 clones. After
removing vector sequence and quality checkingthrough thePhred
program (trim_alt 0.05 (P-score>20), 1039 sequences were generated.
The average length of insert was 792 bp. In addition, we identified 162
clusters, 167 contigs and 391 contigs after clustering and assembling
process using a TGICL package. EST sequences were searchedagainst
NCBI nr database by local BLAST (blastx, E
Abstract: In this paper, backup and recovery technique for Peer
to Peer applications, such as a distributed asynchronous Web-Based
Training system that we have previously proposed. In order to
improve the scalability and robustness of this system, all contents and
function are realized on mobile agents. These agents are distributed
to computers, and they can obtain using a Peer to Peer network
that modified Content-Addressable Network. In the proposed system,
although entire services do not become impossible even if some
computers break down, the problem that contents disappear occurs
with an agent-s disappearance. As a solution for this issue, backups
of agents are distributed to computers. If a failure of a computer is
detected, other computers will continue service using backups of the
agents belonged to the computer.
Abstract: Aerial and satellite images are information rich. They are also complex to analyze. For GIS systems, many features require fast and reliable extraction of roads and intersections. In this paper, we study efficient and reliable automatic extraction algorithms to address some difficult issues that are commonly seen in high resolution aerial and satellite images, nonetheless not well addressed in existing solutions, such as blurring, broken or missing road boundaries, lack of road profiles, heavy shadows, and interfering surrounding objects. The new scheme is based on a new method, namely reference circle, to properly identify the pixels that belong to the same road and use this information to recover the whole road network. This feature is invariable to the shape and direction of roads and tolerates heavy noise and disturbances. Road extraction based on reference circles is much more noise tolerant and flexible than the previous edge-detection based algorithms. The scheme is able to extract roads reliably from images with complex contents and heavy obstructions, such as the high resolution aerial/satellite images available from Google maps.
Abstract: Prickly pear fruit (Opuntia ficus indica L. Miller) belongs to the Cactaceae family. This species is very sensitive to low storage temperatures (< 5°C) which cause damages. The fruits can be peeled, suitably packaged and successfully commercialized as a ready-to-eat product. The main limit to the extension of the shelf life is the production of off-flavors due to different factors, the growth of microorganisms and the action of endogenous enzymes. Lipoxygenase (LOX) and Pectinesterase (PE) are involved in fruit degradation. In particular, LOX pathway is directly responsible for lipid oxidation, and the subsequent production of off-flavours, while PE causes the softening of fruit during maturation. They act on the texture and shelf-life of post-harvest, packaged fruits, as a function of the the grown of microorganisms and packaging technologies used. The aim of this work is to compare the effect of different packaging technologies on the shelf life extension of ready-to-eat prickly pear fruits with regards for the enzymes activities.
Abstract: Highly pathogenic avian influenza (HPAI) H5N1 viruses have created demand for a cost-effective vaccine to prevent a pandemic of the disease. Here, we report that Trichoplusia ni (T. ni) larvae can act as a cost-effective bioreactor to produce recombinant HA5 (rH5HA) proteins as an potential effective vaccine for chickens. To facilitate the recombinant virus identification, virus titer determination and access the infected larvae, we employed the internal ribosome entry site (IRES) derived from Perina nuda virus (PnV, belongs to insect picorna like Iflavirus genus) to construct a bi-cistronic baculovirus expression vector that can express the rH5HA protein and enhanced green fluorescent protein (EGFP) simultaneously. Western blot analysis revealed that the 70 kDa rH5HA protein and partially cleaved products (40 kDa H5HA1) were generated in T. ni larvae infected with recombinant baculovirus carrying the H5HA gene. These data suggest that the baculovirus-larvae recombinant protein expression system could be a cost-effective platform for H5N1 vaccine production.
Abstract: The research focuses on the effects of polyphenols
extracted from Sambucus nigra fruit, using an experimental arterial
hypertension pattern, as well as their influence on the oxidative
stress. The results reveal the normalization of the reduced glutathion
concentration, as well as a considerable reduction in the
malondialdehide serum concentration by the polyphenolic protection.
The rat blood pressure values were recorded using a CODATM
system, which uses a non-invasive blood pressure measuring method.
All the measured blood pressure components revealed a biostatistically
significant (p
Abstract: Multicast transmissions allow an host (the source) to send only one flow bound for a group of hosts (the receivers). Any equipment eager to belong to the group may explicitly register itself to that group via its multicast router. This router will be given the responsibility to convey all information relating to the group to all registered hosts. However in an environment in which the final receiver or the source frequently moves, the multicast flows need particular treatment. This constitutes one of the multicast transmissions problems around which several proposals were made in the Mobile IPv6 case in general. In this article, we describe the problems involved in this IPv6 multicast mobility and the existing proposals for their resolution. Then architecture will be proposed aiming to satisfy and optimize these transmissions in the specific case of a mobile multicast receiver in NC-HMIPv6 environment.
Abstract: Stevia rebaudiana Bertoni (natural sweetener) belongs
to Asteraceae family and can be used as substitute of artificial
sweeteners for diabetic patients. Conventionally, it is cultivated by
seeds or stem cutting, but seed viability rate is poor. A protocol for
callus induction and multiplication was developed to produce large
no. of calli in short period. Surface sterilized nodal, leaf and root
explants were cultured on Murashige and Skoog (MS) medium with
different concentrations of plant hormone like, IBA, kinetin, NAA,
2,4-D, and NAA in combination with 2,4-D. 100% callusing was
observed from leaf explants cultured on combination of NAA and
2,4-D after three weeks while with 2,4-D, only 10% callusing was
observed. Calli obtained from leaf and root explants were shiny green
while with nodal explants it was hard and brown. The present
findings deal with induction of callusing in Stevia to achieve the
rapid callus multiplication for study of steviol glycosides in callus
culture.
Abstract: Post-anthesis drought stress is the most important
problem affecting wheat production in dryland fields, specially in
Mediterranean regions. The main objective of this research was to
evaluate drought tolerance indices in dryland wheat genotypes under
post-anthesis drought stress. The research was including two different
experiments. In each experiment, twenty dryland bread wheat
genotypes were sown in a randomized complete blocks design
(RCBD) with three replications. One of experiments belonged to
rain-fed conditions (post-anthesis drought stress) and other
experiment was under non-stress conditions (with supplemental
irrigation). Different drought tolerance indices include Stress
Tolerance (Tol), Mean Productivity (MP), Geometric Mean
Productivity (GMP), Stress Susceptibility Index (SSI), Stress
Tolerance Index (STI), Harmonic Mean (HAM), Yield Index (YI)
and Yield Stability Index (YSI) were evaluate based on grain yield
under rain-fed (Ys) and supplemental irrigation (Yp) environments.
G10 and G12 were the most tolerant genotypes based on TOL and
SSI. But, based on MP, GMP, STI, HAM and YI indices, G1 and G2
were selected. STI, GMP and MP indices had high correlation with
grain yield under rain-fed and supplementary irrigation conditions
and were recognized as appropriate indices to identify genotypes with
high grain yield and low sensitivity to drought stress environments.
Abstract: Cost contribution arrangements (CCAs) and Cost
sharing agreements (CCAs) belong to the tools of modern finance
management. Costs spend by associated enterprises on developing
producing or obtaining assets, services or rights (in general -
benefits) are used for tax optimizing too. The main purpose of joint
research and development, producing or obtaining benefits is to
lower these costs as much as possible or to maximize the benefits. In
this article is mentioned the problematic of transfer pricing and arm's
length principle with connection of CCAs, CSAs. Next, there is
mentioned how to settle participation shares of the total cost and
benefits contributions with respect to the OECD Transfer pricing for
MNEs Guidelines and with respect to other significant regulations.
Abstract: Nigella sativa L. is an aromatic plant belonging to the
family Ranunculaceae. It has been used traditionally, especially in the
middle East and India, for the treatment of asthma, cough, bronchitis,
headache, rheumatism, fever, influenza and eczema. Several
biological activities have been reported in Nigella sativa seeds,
including antioxidant. In this context we tried to estimate the
antioxidant activity of various extracts prepared from Nigella sativa
seeds, methanolic extract (ME), chloroformic extract (CE), hexanic
extract (HE : fixed oil), ethyl acetate extract (EAE) water extract
(WE). The Folin-Ciocalteu assay showed that CE and EAE contained
high level of phenolic compounds 81.31 and 72.43μg GAE/mg of
extract respectively. Similarly, the CE and EAE exhibited the highest
DPPH radical scavenging activity, with IC50 values of 106.56μg/ml
and 121.62μg/ml respectively. In addition, CE and HE showed the
most scavenging activity against superoxide radical generated in the
PMS-NADH-NBT system with respective IC50 values of 361.86
μg/ml and 371.80 μg/ml, which is comparable to the activity of the
standard antioxidant BHT (344.59 μg/ml). Ferrous ion chelating
capacity assay showed that WE, EAE and ME are the most active
with 40.57, 39.70 and 22.02 mg EDTA-E/g of extract. The inhibition
of linoleic acid/ß-carotene coupled oxidation was estimated by ßcarotene
bleaching assay, this showed a highest relative antioxidant
activity with CE and EAE (69.82% of inhibition). The antioxidant
activities of the methanolic extract and the fixed oil are confirmed by
an in vivo assay in mice, the daily oral administration of methanolic
extract (500 and 800 mg/kg/day) and fixed oil (2 and 4 ml/kg/day)
during 21 days, resulted in a significant enhancement of the blood
total antioxidant capacity (measured by KRL test) and the plasmatic
antioxidant capacity towards DPPH radical.
Abstract: MMR vaccine failure had been reported globally and
here we report that it occurs now in India. Samples were collected from clinically suspected mumps cases were subjected for anti
mumps antibodies, virus isolation, RT-PCR, sequencing and
phylogenetic tree analysis. 56 samples collected from men and women belonging to various age groups. 30 had been vaccinated and
the status of 26 patients was unknown. 28 out of 30 samples were
found to be symptomatic and positive for Mumps IgM, indicating
active mumps infection in 93.4% of the vaccinated population. A
phylogenetic tree comparison of the clinical isolate is shown to be genotype C which is distinct from vaccine strain. Our study clearly sending warning signs that MMR vaccine is a failure and it needs to be revamped for the human use by increasing its efficacy and efficiency.
Abstract: This paper makes a contribution to the on-going
debate on conceptualization and lexicalization of cutting and
breaking (C&B) verbs by discussing data from Telugu, a language of
India belonging to the Dravidian family. Five Telugu native speakers-
verbalizations of agentive actions depicted in 43 short video-clips
were analyzed. It was noted that verbalization of C&B events in
Telugu requires formal units such as simple lexical verbs, explicator
compound verbs, and other complex verb forms. The properties of
the objects involved, the kind of instruments used, and the manner of
action had differential influence on the lexicalization patterns.
Further, it was noted that all the complex verb forms encode 'result'
and 'cause' sub-events in that order. Due to the polysemy associated
with some of the verb forms, our data does not support the
straightforward bipartition of this semantic domain.
Abstract: The great challenge of the agricultural sector is to
produce more crop from less water, which can be achieved by
increasing crop water productivity. The modernization of the
irrigation systems offers a number of possibilities to expand the
economic productivity of water and improve the virtual water status.
The objective of the present study is to assess the global water
productivity (GWP) within the major irrigation command areas of
I.R. Iran. For this purpose, fourteen irrigation command areas where
located in different areas of Iran were selected. In order to calculate
the global water productivity of irrigation command areas, all data on
the delivered water to cropping pattern, cultivated area, crops water
requirement, and yield production rate during 2002-2006 were
gathered. In each of the command areas it seems that the cultivated
crops have a higher amount of virtual water and thus can be replaced
by crops with less virtual water. This is merely suggested due to crop
water consumption and at the time of replacing crops, economic
value as well as cultural and political factors must be considered. The
results indicated that the lowest GWP belongs to Mahyar and
Borkhar irrigation areas, 0.24 kg m-3, and the highest is that of the
Dez irrigation area, 0.81 kg m-3. The findings demonstrated that
water management in the two irrigation areas is just efficient. The
difference in the GWP of irrigation areas is due to variations in the
cropping pattern, amount of crop productions, in addition to the
effective factors in the water use efficiency in the irrigation areas.