Abstract: MicroRNAs (miRNAs) are small, non-coding and
regulatory RNAs about 20 to 24 nucleotides long. Their conserved
nature among the various organisms makes them a good source of
new miRNAs discovery by comparative genomics approach. The
study resulted in 21 miRNAs of 20 pre-miRNAs belonging to 16
families (miR156, 157, 158, 164, 165, 168, 169, 172, 319, 390, 393,
394, 395, 400, 472 and 861) in evergreen spruce tree (Picea). The
miRNA families; miR 157, 158, 164, 165, 168, 169, 319, 390, 393,
394, 400, 472 and 861 are reported for the first time in the Picea. All
20 miRNA precursors form stable minimum free energy stem-loop
structure as their orthologues form in Arabidopsis and the mature
miRNA reside in the stem portion of the stem loop structure. Sixteen
(16) miRNAs are from Picea glauca and five (5) belong to Picea
sitchensis. Their targets consist of transcription factors, growth
related, stressed related and hypothetical proteins.