Characterization of a Novel Galactose-Binding Lectin Homologue from Tenebrio molitor

Year: 2013 Volume: 7 Issue: 9 903 - 905 Pages

• Authors:
• JiEun Jeong
• Dong Hyun Kim
• Bharat Bhusan Patnaik
• Se Won Kang
• HeeJu Hwang
• Yong Hun Jo
• Dae-Hyun Seog
• YeonSooHan
• Yong Seok Lee

Abstract: An expressed sequence tag (EST) analysis provideus portions of expressed genes. We have constructed cDNA library and determined randomly sequences from cDNA library clones of T. molitor injected with acholeplasma lysate. We identified the homologous to a galectin gene. As the result of cloning and characterization of novel, we found that the protein has an open reading frame (ORF) of 495 bp, with 164 amino acid residues and molecular weight of 18.5 kDa. To characterize the role of novel Tm-galectin in immune system, we quantified the mRNA level of galectin at different times after treatment with immune elicitors. The galectin mRNA was up-regulated about 7-folds within 18 hrs. This suggests that Tm-galectin is a novel member of animal lectins, and has a role in the process of pathogen recognition. Our study would be helpful for the study on immune defense system and signaling cascade.

• Keywords:
• EST
• Innate immunity
• Tenebrio molitor
• Galectin.

Performance Evaluation of an Aboveground LNG Storage Tank Cover using Nondestructive and Destructive Tests

Year: 2013 Volume: 7 Issue: 2 135 - 141 Pages

• Authors:
• Sungnam Hong
• Sun-Kyu Park
• Jieun Jeong
• Jinwoong Choi

Abstract: In this study, a new procedure for inspecting damages on LNG storage tanks was proposed with the use of structural diagnostic techniques: i.e., nondestructive inspection techniques such as macrography, the hammer sounding test, the Schmidt hammer test, and the ultrasonic pulse velocity test, and destructive inspection techniques such as the compressive strength test, the chloride penetration test, and the carbonation test. From the analysis of all the test results, it was concluded that the LNG storage tank cover was in good condition. Such results were also compared with the Korean concrete standard specifications and design values. In addition, the remaining life of the LNG storage tank was estimated by using existing models. Based on the results, an LNG storage tank cover performance evaluation procedure was suggested.

• Keywords:
• Destructive test
• LNG storage tank
• Nondestructive test
• Performance evaluation procedure
• Remaining life.

Construction of cDNALibrary and EST Analysis of Tenebriomolitorlarvae

Year: 2011 Volume: 5 Issue: 11 644 - 646 Pages

• Authors:
• JiEun Jeong
• Se-Won Kang
• Hee-Ju Hwang
• Sung-Hwa Chae
• Sang-Haeng Choi
• Hong-SeogPark
• YeonSoo Han
• Bok-Reul Lee
• Dae-Hyun Seog
• Yong Seok Lee

Abstract: Tofurther advance research on immune-related genes from T. molitor, we constructed acDNA library and analyzed expressed sequence taq (EST) sequences from 1,056 clones. After removing vector sequence and quality checkingthrough thePhred program (trim_alt 0.05 (P-score>20), 1039 sequences were generated. The average length of insert was 792 bp. In addition, we identified 162 clusters, 167 contigs and 391 contigs after clustering and assembling process using a TGICL package. EST sequences were searchedagainst NCBI nr database by local BLAST (blastx, E

• Keywords:
• EST
• Innate immunity
• Tenebriomolitor