Abstract: The identification of lipid and soluble sugar components in flour samples of different cultivars belonging to common oat species (Avena sativa L.) was performed: spring oat, winter oat and hulless oat. Fatty acids were extracted from flour samples with n-hexane, and derivatized into volatile methyl esters, using TMSH (trimethylsulfonium hydroxide in methanol). Soluble sugars were then extracted from defatted and dried samples of oat flour with 96% ethanol, and further derivatized into corresponding TMS-oximes, using hydroxylamine hydrochloride solution and BSTFA (N,O-bis-(trimethylsilyl)-trifluoroacetamide). The hexane and ethanol extracts of each oat cultivar were analyzed using GC-MS system. Lipid and simple sugar compositions are very similar in all samples of investigated cultivars. Chemometric tool was applied to numeric values of automatically integrated surface areas of detected lipid and simple sugar components in their corresponding derivatized forms. Hierarchical cluster analysis shows a very high similarity between the investigated flour samples of oat cultivars, according to the fatty acid content (0.9955). Moderate similarity was observed according to the content of soluble sugars (0.50). These preliminary results support the idea of establishing methods for oat flour authentication, and provide the means for distinguishing oat flour samples, regardless of the variety, from flour samples made of other cereal species, just by lipid and simple sugar profile analysis.
Abstract: This study was conducted for the investigation of
number of cellulolytic bacteria and their ability in decomposition.
Seven samples surface soil were collected on cellulose Zailiskii
Alatau slopes. Cellulolitic activity of new strains of Bacillus, isolated
from soil is determined. Isolated cellulose degrading bacteria were
screened for determination of the highest cellulose activity by
quantitative assay using Congo red, gravimetric assay and
colorimetric DNS method trough of the determination of the
parameters of sugar reduction. Strains are assigned to: B.subtilis,
B.licheniformis, B. cereus and, В. megaterium. Bacillus strains
consisting of several different types of cellulases have broad substrate
specificity of cellulase complexes formed by them. Cellulolitic
bacteria were recorded to have highest cellulase activity and selected
for optimization of cellulase enzyme production.
Abstract: Nowadays, food safety is a great public concern;
therefore, robust and effective techniques are required for detecting
the safety situation of goods. Hyperspectral Imaging (HSI) is an
attractive material for researchers to inspect food quality and safety
estimation such as meat quality assessment, automated poultry
carcass inspection, quality evaluation of fish, bruise detection of
apples, quality analysis and grading of citrus fruits, bruise detection
of strawberry, visualization of sugar distribution of melons,
measuring ripening of tomatoes, defect detection of pickling
cucumber, and classification of wheat kernels. HSI can be used to
concurrently collect large amounts of spatial and spectral data on the
objects being observed. This technique yields with exceptional
detection skills, which otherwise cannot be achieved with either
imaging or spectroscopy alone. This paper presents a nonlinear
technique based on kernel Fukunaga-Koontz transform (KFKT) for
detection of fat content in ground meat using HSI. The KFKT which
is the nonlinear version of FKT is one of the most effective
techniques for solving problems involving two-pattern nature. The
conventional FKT method has been improved with kernel machines
for increasing the nonlinear discrimination ability and capturing
higher order of statistics of data. The proposed approach in this paper
aims to segment the fat content of the ground meat by regarding the
fat as target class which is tried to be separated from the remaining
classes (as clutter). We have applied the KFKT on visible and nearinfrared
(VNIR) hyperspectral images of ground meat to determine
fat percentage. The experimental studies indicate that the proposed
technique produces high detection performance for fat ratio in ground
meat.
Abstract: This study investigated the effects of thermal
treatment on Tualang honey sample in terms of honey colour and
heat-induced small metabolites. The heating process was carried out
in a temperature controlled water batch at 90oC for 4 hours. The
honey samples were put in cylinder tubes with the dimension of 1 cm
diameter and 10 cm length for homogenous heat transfer. The results
found that the thermal treatment produced not only
hydroxylmethylfurfural, but also other harmful substances such as
phthalic anhydride and radiolytic byproducts. The degradation of
honey protein was due to the detection of free amino acids such as
cysteine and phenylalanine in heat-treated honey samples. Sugar
dehydration was also occurred because fragmented di-galactose was
identified based on the presence of characteristic ions in the mass
fragmentation pattern. The honey colour was found getting darker as
the heating duration was increased up to 4 hours. Approximately, 60
mm PFund of increment was noticed for the honey colour with the
colour change rate of 14.8 mm PFund per hour. Based on the
principal component analysis, the score plot clearly shows that the
chemical profile of Tualang honey was significantly altered after 2
hours of heating at 90oC.
Abstract: The myocardium is composed of specialized muscle
which relies mainly on fatty acid and sugar metabolism and it is
widely contribute to the heart functioning. The changes of the cardiac
energy-producing system during heart failure have been proved using
autoradiography techniques. This study focused on evaluating sugar
and fatty acid metabolism in myocardium as cardiac energy getting
system using heart-accumulated radiopharmaceuticals. Two sets of
autoradiographs of heart cross sections of Lewis male rats were
analyzed and the time- accumulation curve obtained with use of the
MATLAB image processing software to evaluate fatty acid and sugar
metabolic functions.
Abstract: In order to investigate the prebiotic potential of
oligosaccharides prepared by chemical hydrolysis of water-soluble
polysaccharides (WSP) from Zizyphus lotus leaves, the effect of
oligosaccharides on bacterial growth was studied. The chemical
composition of WSP was evaluated by colorimetric assays revealed
the average values: 7.05±0.73% proteins and 86.21±0.74%
carbohydrates, among them 64.81±0.42% is neutral sugar and the rest
16.25±1.62% is uronic acids. The characterization of
monosaccharides was determined by high performance anion
exchange chromatography with pulsed amperometric detection
(HPAEC-PAD) was found to be composed of galactose (23.95%),
glucose (21.30%), rhamnose (20.28%), arabinose (9.55%), and
glucuronic acid (22.95%). The effects of oligosaccharides on the
growth of lactic acid bacteria were compared with those of fructooligosaccharide
(RP95). The oligosaccharides concentration was
1g/L of Man, Rogosa, Sharpe broth. Bacterial growth was assessed
during 2, 4.5, 6.5, 9, 12, 16 and 24 h by measuring the optical density
of the cultures at 600 nm (OD600) and pH values. During
fermentation, pH in broth cultures decreased from 6.7 to 5.87±0.15.
The enumeration of lactic acid bacteria indicated that
oligosaccharides led to a significant increase in bacteria (P≤0.05)
compared to the control. The fermentative metabolism appeared to be
faster on RP95 than on oligosaccharides from Zizyphus lotus leaves.
Both RP95 and oligosaccharides showed clear prebiotic effects, but
had differences in fermentation kinetics because of to the different
degree of polymerization. This study shows the prebiotic
effectiveness of oligosaccharides, and provides proof for the selection
of leaves of Zizyphus lotus for use as functional food ingredients.
Abstract: The fuel potential of six tropical hardwood species
namely: Triplochiton scleroxylon, Ceiba pentandra, Aningeria
robusta, Terminalia superba, Celtis mildbreadii and Piptadenia
africana were studied. Properties studied included species density,
gross calorific value, volatile matter, ash content, organic carbon and
elemental composition. Fuel properties were determined using
standard laboratory methods. The result indicates that the gross
calorific value (GCV) of the species ranged from 20.16 to 22.22
MJ/kg and they slightly varied from each other. Additionally, the
GCV of the biomass materials were higher than that of other biomass
materials like; wheat straw, rice straw, maize straw and sugar cane.
The ash and volatile matter content varied from 0.6075 to 5.0407%,
and 75.23% to 83.70% respectively. The overall rating of the
properties of the six biomass materials suggested that Piptadenia
africana has the best fuel property to be used as briquettes and
Aningeria robusta the worse. This study therefore suggests that a
holistic assessment of a biomass material needs to be done before
selecting it for fuel purpose.
Abstract: New environmental regulations and the increasing
market preference for companies that respect the ecosystem had
encouraged the industry to look after new treatments for its effluents.
The sugar industry, one of the largest emitter of environmental
pollutants, follows this tendency. Membrane technology is
convenient for separation of suspended solids, colloids and high
molecular weight materials that are present in a wastewater from
sugar industry. The idea is to microfilter the wastewater, where the
permeate passes through the membrane and becomes available for
recycle and re-use in the sugar manufacturing process. For
microfiltration of this effluent a tubular ceramic membrane was used
with a pore size of 200 nm at transmembrane pressure in range of 1–3
bars and in range of flow rate of 50–150 l/h. Kenics static mixer was
used for permeate flux enhancement. Turbidity and suspended solids
were removed and the permeate flux was continuously monitored
during the microfiltration process. The flux achieved after 90 minutes
of microfiltration was in a range of 50–70 l/m2h. The obtained
turbidity decrease was in the range of 50-99 % and total amount of
suspended solids was removed.
Abstract: The phytotoxicity of heavy metals can be expressed
on roots and visible part of plants and is characterized by molecular
and metabolic answers at various levels of organization of the whole
plant. The present study was undertaken on two varieties of broad
bean Vicia faba (Sidi Aïch and Super Aguadulce). The device was
mounted on a substrate prepared by mixing sand, soil and compost,
the substrate was artificially contaminated with three doses of lead
nitrate [Pb(NO3)2] 0, 500 and 1000 ppm. Our objective is to follow
the behavior of plant opposite the stress by evaluating the
physiological parameters. The results reveal a reduction in the
parameters of the productivity (chlorophyll and proteins production)
with an increase in the osmoregulators (soluble sugars and
proline).These results show that the production of broad bean is
strongly modified by the disturbance of its internal physiology under
lead exposure.
Abstract: In this study, lipid-deprived residuals of microalgae
were hydrolyzed for the production of reducing sugars by using the
recombinant Bacillus cellulosome, carrying eight genes from the
Clostridium thermocellum ATCC27405. The obtained cellulosome
was found to exist mostly in the broth supernatant with a cellulosome
activity of 2.4 U/mL. Furthermore, the Michaelis-Menten constant
(Km) and Vmax of cellulosome were found to be 14.832 g/L and 3.522
U/mL. The activation energy of the cellulosome to hydrolyze
microalgae LDRs was calculated as 32.804 kJ/mol.
Abstract: The crude methanol extracts of five indigenous vegetables namely, Amarathus tricolor, Basella rubra L., Chochurus olitorius L., Ipomea batatas, and Momordica chuchinensis L., were examined for their phytochemical profile and antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical. The values for DPPH radical scavenging activity ranged from 7.6-89.53% with B. rubra and I. batatas having the lowest and highest values, respectively. The total flavonoid content of all five indigenous vegetables ranged from 74.65-277.3 mg quercetin equivalent per gram of dried vegetable material while the total phenolic content ranged from 1.93-6.15 mg gallic acid equivalent per gram dried material. Phytochemical screening revealed the presence of steroids, flavonoids, saponins, tannins, carbohydrates and reducing sugars, which may also be associated with the antioxidant activity shown by these indigenous vegetables.
Abstract: Because blueberries are worldwide recognized as a
good source of beneficial components, their consumption has
increased in the past decades, and so have the scientific works about
their properties. Hence, this work was undertaken to evaluate the
effect of some production and conservation factors on the properties
of blueberries from cultivar Bluecrop. The physical and chemical
analyses were done according to established methodologies and then
all data was treated using software SPSS for assessment of the
possible differences among the factors investigated and/or the
correlations between the variables at study. The results showed that
location of production influenced some of the berries properties
(caliber, sugars, antioxidant activity, color and texture) and that the
age of the bushes was correlated with moisture, sugars and acidity, as
well as lightness. On the other hand, altitude of the farm only was
correlated to sugar content. With regards to conservation, it
influenced only anthocyanins content and DPPH antioxidant activity.
Finally, the type of extract and the order of extraction had a
pronounced influence on all the phenolic properties evaluated.
Abstract: The comprehensive CFD models have been used to
represent and study the heterogeneous combustion of biomass. In the
present work, the operation of a global flue gas circuit in the sugarcane
bagasse combustion, from wind boxes below primary air grate
supply, passing by bagasse insertion in swirl burners and boiler
furnace, to boiler bank outlet is simulated. It uses five different
meshes representing each part of this system located in sequence:
wind boxes and grate, boiler furnace, swirl burners, superheaters and
boiler bank. The model considers turbulence using standard k-ε,
combustion using EDM, radiation heat transfer using DTM with 16
ray directions and bagasse particle tracking represented by Schiller-
Naumann model. The results showed good agreement with expected
behavior found in literature and equipment design. The more detailed
results view in separated parts of flue gas system allows observing
some flow behaviors that cannot be represented by usual
simplifications like bagasse supply under homogeneous axial and
rotational vectors and others that can be represented using new
considerations like the representation of 26 thousand grate orifices by
144 rectangular inlets.
Abstract: A comprehensive CFD model is developed to
represent heterogeneous combustion and two burner designs of
supply sugar-cane bagasse into a furnace. The objective of this work
is to compare the insertion and burning of a Brazilian south-eastern
sugar-cane bagasse using a new swirl burner design against an actual
geometry under operation. The new design allows control the
particles penetration and scattering inside furnace by adjustment of
axial/tangential contributions of air feed without change their mass
flow. The model considers turbulence using RNG k-, combustion
using EDM, radiation heat transfer using DTM with 16 ray directions
and bagasse particle tracking represented by Schiller-Naumann
model. The obtained results are favorable to use of new design swirl
burner because its axial/tangential control promotes more penetration
or more scattering than actual design and allows reproduce the actual
design operation without change the overall mass flow supply.
Abstract: In this work, two fermentations at different
temperatures (25 and 30ºC), with cell recycling, were accomplished
to produce ethanol, using a mix of commercial substrates, xylose
(70%) and glucose (30%), as organic source for Scheffersomyces
stipitis. Five consecutive fermentations of 80 g L-1 (1º, 2º and 3º
recycles), 96 g L-1 (4º recycle) and 120 g L-1 (5º recycle)reduced
sugars led to a final maximum ethanol concentration of 17.2 and 34.5
g L-1, at 25 and 30ºC, respectively. Glucose was the preferred
substrate; moreover xylose startup degradation was initiated after a
remaining glucose presence in the medium. Results showed that yeast
acid treatment, performed before each cycle, provided improvements
on cell viability, accompanied by ethanol productivity of 2.16 g L-1 h-
1 at 30ºC. A maximum 36% of xylose was retained in the
fermentation medium and after five-cycle fermentation an ethanol
yield of 0.43 g ethanol/g sugars was observed. S. stipitis fermentation
capacity and tolerance showed better results at 30ºC with 83.4% of
theoretical yield referenced on initial biomass.
Abstract: Lignocellolusic material is a substance that is resistant to be degraded by microorganisms or hydrolysis enzymes. To be used as materials for biofuel production, it needs pretreatment process to improve efficiency of hydrolysis. In this work, chemical pretreatments on rice straw using three diluted organic acids, including acetic acid, citric acid, oxalic acid, were optimized. Using Response Surface Methodology (RSM), the effect of three pretreatment parameters, acid concentration, treatment time, and reaction temperature, on pretreatment efficiency were statistically evaluated. The results indicated that dilute oxalic acid pretreatment led to the highest enhancement of enzymatic saccharification by commercial cellulase and yielded sugar up to 10.67 mg/ml when using 5.04% oxalic acid at 137.11 oC for 30.01 min. Compared to other acid pretreatment by acetic acid, citric acid, and hydrochloric acid, the maximum sugar yields are 7.07, 6.30, and 8.53 mg/ml, respectively. Here, it was demonstrated that organic acids can be used for pretreatment of lignocellulosic materials to enhance of hydrolysis process, which could be integrated to other applications for various biorefinery processes.
Abstract: Control of honey frauds is needed in Ecuador to
protect bee keepers and consumers because simple syrups and new
syrups with eucalyptus are sold as genuine honeys. Authenticity of
Ecuadorian commercial honeys was tested with a vortex emulsion
consisting on one volume of honey:water (1:1) dilution, and two
volumes of diethyl ether. This method allows a separation of phases
in one minute to discriminate genuine honeys that form three phase
and fake honeys that form two phases; 34 of the 42 honeys analyzed
from five provinces of Ecuador were genuine. This was confirmed
with 1H NMR spectra of honey dilutions in deuterated water with an
enhanced amino acid region with signals for proline, phenylalanine
and tyrosine. Classic quality indicators were also tested with this
method (sugars, HMF), indicators of fermentation (ethanol, acetic
acid), and residues of citric acid used in the syrup manufacture. One
of the honeys gave a false positive for genuine, being an admixture of
genuine honey with added syrup, evident for the high sucrose.
Sensory analysis was the final confirmation to recognize the honey
groups studied here, namely honey produced in combs by Apis
mellifera, fake honey, and honey produced in cerumen pots by
Geotrigona, Melipona, and Scaptotrigona. Chloroform extractions of
honey were also done to search lipophilic additives in NMR spectra.
This is a valuable contribution to protect honey consumers, and to
develop the beekeeping industry in Ecuador.
Abstract: The biodegradable family of polymers
polyhydroxyalkanoates is an interesting substitute for convectional
fossil-based plastics. However, the manufacturing and environmental
impacts associated with their production via intracellular bacterial
fermentation are strongly dependent on the raw material used and on
energy consumption during the extraction process, limiting their
potential for commercialization. Industrial wastewater is studied in
this paper as a promising alternative feedstock for waste valorization.
Based on results from laboratory and pilot-scale experiments, a
conceptual process design, techno-economic analysis and life cycle
assessment are developed for the large-scale production of the most
common type of polyhydroxyalkanoate, polyhydroxbutyrate.
Intracellular polyhydroxybutyrate is obtained via fermentation of
microbial community present in industrial wastewater and the
downstream processing is based on chemical digestion with
surfactant and hypochlorite. The economic potential and
environmental performance results help identifying bottlenecks and
best opportunities to scale-up the process prior to industrial
implementation. The outcome of this research indicates that the
fermentation of wastewater towards PHB presents advantages
compared to traditional PHAs production from sugars because the
null environmental burdens and financial costs of the raw material in
the bioplastic production process. Nevertheless, process optimization
is still required to compete with the petrochemicals counterparts.
Abstract: The purpose of the present work was to develop an
innovative food product with nutritional properties as well as
appealing organoleptic qualities. The product, a jam, was prepared
with the beans’ cooking water combined with fresh apple or carrot,
without the addition of any conservatives. Three different jams were
produced: bean and carrot, bean and apple and bean, apple and
cinnamon. The developed products underwent a sensorial analysis
that revealed that the bean, apple and cinnamon jam was globally
better accepted. However, with this study, the consumers determined
that the bean and carrot jam had the most attractive color and the
bean and apple jam the better consistency. Additionally, it was
possible to analyze the jams for their chemical components, namely
fat, fiber, protein, sugars and antioxidant activity. The obtained
results showed that the bean and carrot jam had the highest lipid
content, while the bean, apple and cinnamon jam had the highest
fiber content, when compared to the other two jams. Regarding the
sugar content, both jams with apple revealed similar sugar values,
which were higher than the sugar content of the bean and carrot jam.
The antioxidant activity was on average 10 mg TE/g.
Abstract: The objective of this study was to identify the optimal
level of partial replacement of Portland cement by the ashes
originating from burning straw and bagasse from sugar cane (ASB).
Order to this end, were made five series of flat plates and cylindrical
bodies: control and others with the partial replacement in 20, 30, 40
and 50% of ASB in relation to the mass of the Ordinary Portland
cement, and conducted a mechanical testing of simple axial
compression (cylindrical bodies) and the four-point bending (flat
plates) and determined water absorption (WA), bulk density (BD)
and apparent void volume (AVV) on both types of specimens. Based
on the data obtained, it may be noted that the control treatment
containing only Portland cement, obtained the best results. However,
the cylindrical bodies with 20% ashes showed better results
compared to the other treatments. And in the formulations plates, the
treatment which showed the best results was 30% cement
replacement by ashes.