Fermentation of Xylose and Glucose Mixture in Intensified Reactors by Scheffersomyces stipitis to Produce Ethanol
In this work, two fermentations at different
temperatures (25 and 30ºC), with cell recycling, were accomplished
to produce ethanol, using a mix of commercial substrates, xylose
(70%) and glucose (30%), as organic source for Scheffersomyces
stipitis. Five consecutive fermentations of 80 g L-1 (1º, 2º and 3º
recycles), 96 g L-1 (4º recycle) and 120 g L-1 (5º recycle)reduced
sugars led to a final maximum ethanol concentration of 17.2 and 34.5
g L-1, at 25 and 30ºC, respectively. Glucose was the preferred
substrate; moreover xylose startup degradation was initiated after a
remaining glucose presence in the medium. Results showed that yeast
acid treatment, performed before each cycle, provided improvements
on cell viability, accompanied by ethanol productivity of 2.16 g L-1 h-
1 at 30ºC. A maximum 36% of xylose was retained in the
fermentation medium and after five-cycle fermentation an ethanol
yield of 0.43 g ethanol/g sugars was observed. S. stipitis fermentation
capacity and tolerance showed better results at 30ºC with 83.4% of
theoretical yield referenced on initial biomass.
[1] A. Gupta and J.P. Verma. (2015) “Sustainable bio-ethanol production
from agro-residues: A review”. Renew Sustain Energy Reviews, 41: 550-
67, 2015.
[2] B. Gutiérrez-Rivera, B. Ortiz-Muñiz, J. Gómez-Rodríguez, et al. (2015)
“Bioethanol production from hydrolyzed sugarcane bagasse
supplemented with molasses “B” in a mixed yeast culture”. Renew
Energy, 74: 399-405.
[3] R. den Hann, E. van Rensburg, S.H. Rose, et al. (2015) “Progress and
challenges in the engineering of non-cellulolytic microorganisms for
consolidated bioprocessing” Current Opinion Biotechnol, 33:32-8.
[4] M. Liang, M.H. Kim, Q.P. HE, et al. (2013) “Impact of pseudocontinuous
fermentation on the ethanol tolerance of Scheffersomyces
stipitis. J Bioscien Bioengin, 116:319-326.
[5] J. Shi, M. Zhang, L. Zhang, P. Wang, et al. (2014) “Xylose-fermenting
Pichia stipitis by genome shuffling for improved ethanol production”.
Microbial Biotechnol, 7:90-9.
[6] T. C. Yang, J. Kumaran, S. Amartey, et al. (2014) “Chapter 5 - Biofuels
and Bioproducts Produced through Microbial Conversion of Biomass”.
Bioenergy Research: Advances and Applications, 71-93.
[7] P.J. Slininger, S.R.Thompson, S. Weber, et al. (2011) “Repression of
xylose-specific enzymes by ethanol in Scheffersomyces (Pichia) stipitis
and utility of repitching xylose-grown populations to eliminate diauxic
lag". Biotechnol Bioengin, 8:1801-15.
[8] F.K. Agbogbo, F.D. Haagensen, D. Milam and K.S. Wenger (2008)
“Fermentation of acid-pretreatment corn stover to ethanol without
detoxification using Pichia stipitis”. Appl Biochem Biotechnol, 145:53-
8.
[9] M. Bertilsson, J. Andersson and G. Liden (2008) “Modeling
simultaneous glucose and xylose uptake in Saccharomyces cerevisiae
from kinetics and gene expression of sugar transporters”. Bioprocess
Biosyst Eng., 31:369-377.
[10] P.K. Bajwa, T. Shireen, F. D’Aoust, et al. (2009) “Mutants of the
pentose-fermenting yeast Pichia stipitis with improved tolerance to
inhibitors in hardwood spent sulfite liquor”. Biotechnol Bioengin,
104:892-900.
[11] A.M.R.B Xavier, C.J.R. Frazão, S.R. Pereira,et al. (2014) “Bioethanol
Production: Adaptation of Scheffersomyces stipitis to Hardwood Spent
Sulfite Liquor”. New Biotechnol, 31:S101
[12] I.C. Roberto, L.S. Lack, M.F.S. Barbosa and I.M. de Mancilha (1991).
“Utilization of Sugar Cane Bagasse Hemicellulosic Hydrolysate by
Pichia stipitis for the Production of Ethanol” Process Biochem, 26:15-
21.
[13] L. Canilha, W. Carvalho, M.G.A. Felipe, et al. (2010) “Ethanol
Production from Sugarcane Bagasse Hydrolysate Using Pichia stipitis.
Appl Biochem Biotechnol, 161:84-92.
[14] A.D. Ferreira, S.I. Mussatto, R.M. Cadete, et al. (2011) “Ethanol
production by a new pentose-fermenting yeast strain, Scheffersomyces
stipitis UFMG-IMH 43.2, isolated from the Brazilian forest”. Yeast,
28:547-554.
[15] R. Gupta, G. Mehta and G. Kuhad (2012). Fermentation of pentose and
hexose sugars from corncob, a low cost feedstock into ethanol. Biomass
Bioenergy, 47: 334-341.
[16] D. Scordia, S.L. Cosentino, J.W. Lee and T.W. Jeffries (2012)
“Bioconversion of giant reed (Arundo donax L.) hemicellulose
hydrolysate to ethanol by Scheffersomyces stipitis CBS6054”. Biomass
Bioenergy, 39: 296-305.
[17] T.H., Lin, C.F. Huang, G.L. Guo, et al. (2012) “Pilot-scale ethanol
production from rice straw hydrolysates using xylose-fermenting Pichia
stipitis. Bioresour Technol, 116:314-319.
[18] A. Singh, S. Bajar and N.R. Bishnoi (2014) “Enzymatic hydrolysis of
microwave alkali pretreated rice husk for ethanol production by
Saccharomyces cerevisiae, Scheffersomyces stipitis and their coculture”.
Fuel, 116:699-702.
[19] B. Erdei, B. Frankó, M. Galbe, G. Zacchi (2013) “Glucose and xylose
co-fermentation of pretreated wheat straw using mutants of S. cerevisiae
TMB3400”. J Biotechnol 164:50-58.
[20] K.K. Cheng, B.Y. Cai, J.A. Zhang, et al. (2008) “Sugarcane bagasse
hemicellulose hydrolysate for ethanol production by acid recovery
process”. Biochemical Engineerin J, 38:105-9.
[21] P.K. Bajwa, D. Pinel, V.J.J. Martin, et al. (2010) “Strain improvement of
the pentose-fermenting yeast Pichia stipitis by genome shuffling”. J
Microbiologic Methods, 81:179-186.
[22] M. Galbe and G. Zazzhi (2012) “Pretreatment: the key to efficient
utilization of lignocellulosic materials”. Biomass and Bioenergy, 46:70-
78.
[23] P.K. Bajwa, C. Phaenark, N. Grant, et al. (2011) “Ethanol production
from selected lignocellulosic hydrolysates by genome shuffled strains of
Scheffersomyces stipitis”. Bioresour Technol, 102:9965-9.
[24] L.C. Basso, T.O. Basso and S.N. Rocha (2011) “Biofuel Production –
Recent Developments and Prospects” Chapter - Ethanol Production in
Brazil: The Industrial Process and Its Impact on Yeast Fermentation, p.
85-100.
[25] R.R. de Andrade, F.M. Filho, R.M. Filho, A.C. da Costa (2013).
“Kinetics of ethanol production from sugarcane bagasse enzymatic
hydrolysate concentrated with molasses under cell recycle”. Bioresour
Technol, 130:351-9.
[26] R. Landaeta, G. Aroca, F. Azevedo, J.A. Teixeira, S.I. Mussato (2013)
“Adaptation of a flocculent Saccharomyces cerevisiae strain to
lignocellulosic inhibitors by cell recycle batch fermentation. Applied
Energy, 102:124-130.
[27] Y. Matano, T. Hasunuma, A. Kondo (2013) “Cell recycle batch
fermentation of high-solid lignocellulose using a recombinant cellulasedisplaying
yeast strain for high yield ethanol production in consolidated
bioprocessing”. Bioresource Technology, 135:403-409.
[28] J.P.A. Silva, S.I, Mussato, I.C. Roberto, et al. (2012) “Fermentation
medium and oxygen transfer conditions that maximize the xylose
conversion to ethanol by Pichia stipitis. Renewable Energy, 37:259-265.
[29] J.M. Gancedo (1992) Carbon catabolite repression in yeast. Eur J
Biochem,. 206: 297-313. [30] M.J. Taherzadeh and K. Karimi (2011) “Chapter 12 - Fermentation
Inhibitors in Ethanol Processes and Different Strategies to Reduce Their
Effects”. Biofuels, 287-311.
[31] P. Phitsuwan, K. Sakka, K. Ratanakhanokchai (2013) “Improvement of
lignocellulosic biomass in planta: A review of feedstocks, biomass
recalcitrance, and strategic manipulation of ideal plants designed for
ethanol production and processability” Biomass and Bioenergy, 58:390-
405.
[1] A. Gupta and J.P. Verma. (2015) “Sustainable bio-ethanol production
from agro-residues: A review”. Renew Sustain Energy Reviews, 41: 550-
67, 2015.
[2] B. Gutiérrez-Rivera, B. Ortiz-Muñiz, J. Gómez-Rodríguez, et al. (2015)
“Bioethanol production from hydrolyzed sugarcane bagasse
supplemented with molasses “B” in a mixed yeast culture”. Renew
Energy, 74: 399-405.
[3] R. den Hann, E. van Rensburg, S.H. Rose, et al. (2015) “Progress and
challenges in the engineering of non-cellulolytic microorganisms for
consolidated bioprocessing” Current Opinion Biotechnol, 33:32-8.
[4] M. Liang, M.H. Kim, Q.P. HE, et al. (2013) “Impact of pseudocontinuous
fermentation on the ethanol tolerance of Scheffersomyces
stipitis. J Bioscien Bioengin, 116:319-326.
[5] J. Shi, M. Zhang, L. Zhang, P. Wang, et al. (2014) “Xylose-fermenting
Pichia stipitis by genome shuffling for improved ethanol production”.
Microbial Biotechnol, 7:90-9.
[6] T. C. Yang, J. Kumaran, S. Amartey, et al. (2014) “Chapter 5 - Biofuels
and Bioproducts Produced through Microbial Conversion of Biomass”.
Bioenergy Research: Advances and Applications, 71-93.
[7] P.J. Slininger, S.R.Thompson, S. Weber, et al. (2011) “Repression of
xylose-specific enzymes by ethanol in Scheffersomyces (Pichia) stipitis
and utility of repitching xylose-grown populations to eliminate diauxic
lag". Biotechnol Bioengin, 8:1801-15.
[8] F.K. Agbogbo, F.D. Haagensen, D. Milam and K.S. Wenger (2008)
“Fermentation of acid-pretreatment corn stover to ethanol without
detoxification using Pichia stipitis”. Appl Biochem Biotechnol, 145:53-
8.
[9] M. Bertilsson, J. Andersson and G. Liden (2008) “Modeling
simultaneous glucose and xylose uptake in Saccharomyces cerevisiae
from kinetics and gene expression of sugar transporters”. Bioprocess
Biosyst Eng., 31:369-377.
[10] P.K. Bajwa, T. Shireen, F. D’Aoust, et al. (2009) “Mutants of the
pentose-fermenting yeast Pichia stipitis with improved tolerance to
inhibitors in hardwood spent sulfite liquor”. Biotechnol Bioengin,
104:892-900.
[11] A.M.R.B Xavier, C.J.R. Frazão, S.R. Pereira,et al. (2014) “Bioethanol
Production: Adaptation of Scheffersomyces stipitis to Hardwood Spent
Sulfite Liquor”. New Biotechnol, 31:S101
[12] I.C. Roberto, L.S. Lack, M.F.S. Barbosa and I.M. de Mancilha (1991).
“Utilization of Sugar Cane Bagasse Hemicellulosic Hydrolysate by
Pichia stipitis for the Production of Ethanol” Process Biochem, 26:15-
21.
[13] L. Canilha, W. Carvalho, M.G.A. Felipe, et al. (2010) “Ethanol
Production from Sugarcane Bagasse Hydrolysate Using Pichia stipitis.
Appl Biochem Biotechnol, 161:84-92.
[14] A.D. Ferreira, S.I. Mussatto, R.M. Cadete, et al. (2011) “Ethanol
production by a new pentose-fermenting yeast strain, Scheffersomyces
stipitis UFMG-IMH 43.2, isolated from the Brazilian forest”. Yeast,
28:547-554.
[15] R. Gupta, G. Mehta and G. Kuhad (2012). Fermentation of pentose and
hexose sugars from corncob, a low cost feedstock into ethanol. Biomass
Bioenergy, 47: 334-341.
[16] D. Scordia, S.L. Cosentino, J.W. Lee and T.W. Jeffries (2012)
“Bioconversion of giant reed (Arundo donax L.) hemicellulose
hydrolysate to ethanol by Scheffersomyces stipitis CBS6054”. Biomass
Bioenergy, 39: 296-305.
[17] T.H., Lin, C.F. Huang, G.L. Guo, et al. (2012) “Pilot-scale ethanol
production from rice straw hydrolysates using xylose-fermenting Pichia
stipitis. Bioresour Technol, 116:314-319.
[18] A. Singh, S. Bajar and N.R. Bishnoi (2014) “Enzymatic hydrolysis of
microwave alkali pretreated rice husk for ethanol production by
Saccharomyces cerevisiae, Scheffersomyces stipitis and their coculture”.
Fuel, 116:699-702.
[19] B. Erdei, B. Frankó, M. Galbe, G. Zacchi (2013) “Glucose and xylose
co-fermentation of pretreated wheat straw using mutants of S. cerevisiae
TMB3400”. J Biotechnol 164:50-58.
[20] K.K. Cheng, B.Y. Cai, J.A. Zhang, et al. (2008) “Sugarcane bagasse
hemicellulose hydrolysate for ethanol production by acid recovery
process”. Biochemical Engineerin J, 38:105-9.
[21] P.K. Bajwa, D. Pinel, V.J.J. Martin, et al. (2010) “Strain improvement of
the pentose-fermenting yeast Pichia stipitis by genome shuffling”. J
Microbiologic Methods, 81:179-186.
[22] M. Galbe and G. Zazzhi (2012) “Pretreatment: the key to efficient
utilization of lignocellulosic materials”. Biomass and Bioenergy, 46:70-
78.
[23] P.K. Bajwa, C. Phaenark, N. Grant, et al. (2011) “Ethanol production
from selected lignocellulosic hydrolysates by genome shuffled strains of
Scheffersomyces stipitis”. Bioresour Technol, 102:9965-9.
[24] L.C. Basso, T.O. Basso and S.N. Rocha (2011) “Biofuel Production –
Recent Developments and Prospects” Chapter - Ethanol Production in
Brazil: The Industrial Process and Its Impact on Yeast Fermentation, p.
85-100.
[25] R.R. de Andrade, F.M. Filho, R.M. Filho, A.C. da Costa (2013).
“Kinetics of ethanol production from sugarcane bagasse enzymatic
hydrolysate concentrated with molasses under cell recycle”. Bioresour
Technol, 130:351-9.
[26] R. Landaeta, G. Aroca, F. Azevedo, J.A. Teixeira, S.I. Mussato (2013)
“Adaptation of a flocculent Saccharomyces cerevisiae strain to
lignocellulosic inhibitors by cell recycle batch fermentation. Applied
Energy, 102:124-130.
[27] Y. Matano, T. Hasunuma, A. Kondo (2013) “Cell recycle batch
fermentation of high-solid lignocellulose using a recombinant cellulasedisplaying
yeast strain for high yield ethanol production in consolidated
bioprocessing”. Bioresource Technology, 135:403-409.
[28] J.P.A. Silva, S.I, Mussato, I.C. Roberto, et al. (2012) “Fermentation
medium and oxygen transfer conditions that maximize the xylose
conversion to ethanol by Pichia stipitis. Renewable Energy, 37:259-265.
[29] J.M. Gancedo (1992) Carbon catabolite repression in yeast. Eur J
Biochem,. 206: 297-313. [30] M.J. Taherzadeh and K. Karimi (2011) “Chapter 12 - Fermentation
Inhibitors in Ethanol Processes and Different Strategies to Reduce Their
Effects”. Biofuels, 287-311.
[31] P. Phitsuwan, K. Sakka, K. Ratanakhanokchai (2013) “Improvement of
lignocellulosic biomass in planta: A review of feedstocks, biomass
recalcitrance, and strategic manipulation of ideal plants designed for
ethanol production and processability” Biomass and Bioenergy, 58:390-
405.
@article{"International Journal of Biological, Life and Agricultural Sciences:69976", author = "S. C. Santos and S. R. Dionísio and A. L. D. De Andrade and L. R. Roque and A. C. Da Costa and J. L. Ienczak", title = "Fermentation of Xylose and Glucose Mixture in Intensified Reactors by Scheffersomyces stipitis to Produce Ethanol", abstract = "In this work, two fermentations at different
temperatures (25 and 30ºC), with cell recycling, were accomplished
to produce ethanol, using a mix of commercial substrates, xylose
(70%) and glucose (30%), as organic source for Scheffersomyces
stipitis. Five consecutive fermentations of 80 g L-1 (1º, 2º and 3º
recycles), 96 g L-1 (4º recycle) and 120 g L-1 (5º recycle)reduced
sugars led to a final maximum ethanol concentration of 17.2 and 34.5
g L-1, at 25 and 30ºC, respectively. Glucose was the preferred
substrate; moreover xylose startup degradation was initiated after a
remaining glucose presence in the medium. Results showed that yeast
acid treatment, performed before each cycle, provided improvements
on cell viability, accompanied by ethanol productivity of 2.16 g L-1 h-
1 at 30ºC. A maximum 36% of xylose was retained in the
fermentation medium and after five-cycle fermentation an ethanol
yield of 0.43 g ethanol/g sugars was observed. S. stipitis fermentation
capacity and tolerance showed better results at 30ºC with 83.4% of
theoretical yield referenced on initial biomass.", keywords = "5-carbon sugar, cell recycling fermenter, mixed
sugars, xylose-fermenting yeast.", volume = "9", number = "5", pages = "539-6", }
