Abstract: COPD is characterized by loss of elastic fibers from
small airways and alveolar walls, with the decrease in elastin
increasing with disease severity. It is unclear why there is a lack of
repair of elastic fibers. We have examined fibroblasts cultured from
lung tissue from normal and COPD subjects to determine if the
secretory profile explains lack of tissue repair. In this study,
fibroblasts were cultured from lung parenchyma of bronchial
carcinoma patients with varying degrees of COPD; controls
(non-COPD, n=5), mild COPD (GOLD 1, n=5) and moderate-severe
COPD (GOLD 2-3, n=12). Measurements were made of proliferation,
senescence-associated beta-galactosidase-1, mRNA expression of
IL-6, IL-8, MMP-1, tropoelastin and versican, and protein levels for
IL-6, IL-8, PGE2, tropoelastin, insoluble elastin, and versican. It was
found that GOLD 2-3 fibroblasts proliferated more slowly (p
Abstract: Recently, there has been a considerable increase in the
number of procedures carried out under regional anesthesia.
However, percutaneous nephrolithotomy (PCNL) procedures are
usually performed under general anesthesia. The aim of this study
was to assess the safety and efficacy of PCNL under spinal anesthesia
in patients with renal calculi. We describe our 9 years experience of
performing PCNL under spinal anesthesia for 387 patients with large
stones of the upper urinary tract, with regard to the effectiveness and
side effects. All patients received spinal anesthetics (Lidocain 5%, or
Bupivacaine 0.75%) and underwent PCNL in prone position. The
success rate was 94.1%. The incidence of complications was 11.6%.
PCNL under spinal anesthesia is feasible, safe, and well-tolerated in
management of patients with renal stones.
Abstract: Abdominal aortic aneurysms rupture (AAAs) is one of the main causes of death in the world. This is a very complex phenomenon that usually occurs “without previous warning". Currently, criteria to assess the aneurysm rupture risk (peak diameter and growth rate) can not be considered as reliable indicators. In a first approach, the main geometric parameters of aneurysms have been linked into five biomechanical factors. These are combined to obtain a dimensionless rupture risk index, RI(t), which has been validated preliminarily with a clinical case and others from literature. This quantitative indicator is easy to understand, it allows estimating the aneurysms rupture risks and it is expected to be able to identify the one in aneurysm whose peak diameter is less than the threshold value. Based on initial results, a broader study has begun with twelve patients from the Clinic Hospital of Valladolid-Spain, which are submitted to periodic follow-up examinations.
Abstract: Oxidative stress is considered to be the cause for onset
and the progression of type 2 diabetes mellitus (T2DM) and
complications including neuropathy. It is a deleterious process that
can be an important mediator of damage to cell structures: protein,
lipids and DNA. Data suggest that in patients with diabetes and
diabetic neuropathy DNA repair is impaired, which prevents effective
removal of lesions. Objective: The aim of our study was to evaluate
the association of the hOGG1 (326 Ser/Cys) and XRCC1 (194
Arg/Trp, 399 Arg/Gln) gene polymorphisms whose protein is
involved in the BER pathway with DNA repair efficiency in patients
with diabetes type 2 and diabetic neuropathy compared to the healthy
subjects. Genotypes were determined by PCR-RFLP analysis in 385
subjects, including 117 with type 2 diabetes, 56 with diabetic
neuropathy and 212 with normal glucose metabolism. The
polymorphisms studied include codon 326 of hOGG1 and 194, 399
of XRCC1 in the base excision repair (BER) genes. Comet assay was
carried out using peripheral blood lymphocytes from the patients and
controls. This test enabled the evaluation of DNA damage in cells
exposed to hydrogen peroxide alone and in the combination with the
endonuclease III (Nth). The results of the analysis of polymorphism
were statistically examination by calculating the odds ratio (OR) and
their 95% confidence intervals (95% CI) using the ¤ç2-tests. Our data
indicate that patients with diabetes mellitus type 2 (including those
with neuropathy) had higher frequencies of the XRCC1 399Arg/Gln
polymorphism in homozygote (GG) (OR: 1.85 [95% CI: 1.07-3.22],
P=0.3) and also increased frequency of 399Gln (G) allele (OR: 1.38
[95% CI: 1.03-1.83], P=0.3). No relation to other polymorphisms
with increased risk of diabetes or diabetic neuropathy. In T2DM
patients complicated by neuropathy, there was less efficient repair of
oxidative DNA damage induced by hydrogen peroxide in both the
presence and absence of the Nth enzyme. The results of our study
suggest that the XRCC1 399 Arg/Gln polymorphism is a significant
risk factor of T2DM in Polish population. Obtained data suggest a
decreased efficiency of DNA repair in cells from patients with
diabetes and neuropathy may be associated with oxidative stress.
Additionally, patients with neuropathy are characterized by even
greater sensitivity to oxidative damage than patients with diabetes,
which suggests participation of free radicals in the pathogenesis of
neuropathy.
Abstract: There are a number of different cars for transferring hundreds of close contacts of swine influenza patients to hospital, and we need to carefully assign the passengers to those cars in order to minimize the risk of influenza spreading during transportation. The paper presents an approach to straightforward obtain the optimal solution of the relaxed problems, and develops two iterative improvement algorithms to effectively tackle the general problem.
Abstract: Along with the advances in medicine, providing medical information to individual patient is becoming more important. In Japan such information via Braille is hardly provided to blind and partially sighted people. Thus we are researching and developing a Web-based automatic translation program “eBraille" to translate Japanese text into Japanese Braille. First we analyzed the Japanese transcription rules to implement them on our program. We then added medical words to the dictionary of the program to improve its translation accuracy for medical text. Finally we examined the efficacy of statistical learning models (SLMs) for further increase of word segmentation accuracy in braille translation. As a result, eBraille had the highest translation accuracy in the comparison with other translation programs, improved the accuracy for medical text and is utilized to make hospital brochures in braille for outpatients and inpatients.
Abstract: Previous studies have shown that there are arguments
regarding the reliability and validity of the Ashworth and Modified
Ashworth Scale towards evaluating patients diagnosed with upper
limb disorders. These evaluations depended on the raters’ experiences.
This initiated us to develop an upper limb disorder part-task trainer
that is able to simulate consistent upper limb disorders, such as
spasticity and rigidity signs, based on the Modified Ashworth Scale to
improve the variability occurring between raters and intra-raters
themselves. By providing consistent signs, novice therapists would be
able to increase training frequency and exposure towards various
levels of signs. A total of 22 physiotherapists and occupational
therapists participated in the study. The majority of the therapists
agreed that with current therapy education, they still face problems
with inter-raters and intra-raters variability (strongly agree 54%; n =
12/22, agree 27%; n = 6/22) in evaluating patients’ conditions. The
therapists strongly agreed (72%; n = 16/22) that therapy trainees
needed to increase their frequency of training; therefore believe that
our initiative to develop an upper limb disorder training tool will help
in improving the clinical education field (strongly agree and agree
63%; n = 14/22).
Abstract: The main aim is to perform mutational analysis of CTLA4 gene Exon 1 in SLE patients. A total of 61 SLE patients fulfilling “American College of Rheumatology (ACR) criteria" and 61 controls were enrolled in this study. The region of CTLA4 gene exon 1 was amplified by using Step-down PCR technique. Extracted DNA of band 354 bp was sequenced to analyze mutations in the exon-1 of CTLA-4 gene. Further, protein sequences were identified from nucleotide sequences of CTLA4 Exon 1 by using Expasy software and through Blast P software it was found that CTLA4 protein sequences of Pakistani SLE patients were similar to that of Chinese SLE population. No variations were found after patients sequences were compared with that of the control sequence. Furthermore it was found that CTLA4 protein sequences of Pakistani SLE patients were similar to that of Chinese SLE population. Thus CTLA4 gene may not be responsible for an autoimmune disease SLE.
Abstract: Endovascular aneurysm repair is a new and minimally invasive repair for patients with abdominal aortic aneurysm (AAA). This method has potential advantages that are incomparable with other repair methods. However, the enlargement of aneurysm in the absence of endoleak, which is known as endotension, may occur as one of post-operative compliances of this method. Typically, endotension is mainly as a result of pressure transmitted to aneurysm sac by endovascular installed graft. After installation of graft the aneurysm sac reduces significantly but remains non-zero. There are some factors which affect this pressure transmitted. In this study, the geometry features of installed vascular graft have been considered. It is inferred that graft neck angle and iliac bifurcation angle are two factors which can affect the drag force on graft and consequently the pressure transmitted to aneurysm.
Abstract: Background: Blunt aortic trauma (BAT) includes
various morphological changes that occur during deceleration,
acceleration and/or body compression in traffic accidents. The
various forms of BAT, from limited laceration of the intima to
complete transection of the aorta, depends on the force acting on the
vessel wall and the tolerance of the aorta to injury. The force depends
on the change in velocity, the dynamics of the accident and of the
seating position in the car. Tolerance to aortic injury depends on the
anatomy, histological structure and pathomorphological alterations
due to aging or disease of the aortic wall.
An overview of the literature and medical documentation reveals
that different terms are used to describe certain forms of BAT, which
can lead to misinterpretation of findings or diagnoses. We therefore,
propose a classification that would enable uniform systematic
screening of all forms of BAT. We have classified BAT into three
morphologycal types: TYPE I (intramural), TYPE II (transmural) and
TYPE III (multiple) aortic ruptures with appropriate subtypes.
Methods: All car accident casualties examined at the Institute of
Forensic Medicine from 2001 to 2009 were included in this
retrospective study. Autopsy reports were used to determine the
occurrence of each morphological type of BAT in deceased drivers,
front seat passengers and other passengers in cars and to define the
morphology of BAT in relation to the accident dynamics and the age
of the fatalities.
Results: A total of 391 fatalities in car accidents were included in
the study. TYPE I, TYPE II and TYPE III BAT were observed in
10,9%, 55,6% and 33,5%, respectively. The incidence of BAT in
drivers, front seat and other passengers was 36,7%, 43,1% and
28,6%, respectively. In frontal collisions, the incidence of BAT was
32,7%, in lateral collisions 54,2%, and in other traffic accidents
29,3%. The average age of fatalities with BAT was 42,8 years and of
those without BAT 39,1 years.
Conclusion: Identification and early recognition of the risk factors
of BAT following a traffic accident is crucial for successful treatment
of patients with BAT. Front seat passengers over 50 years of age who
have been injured in a lateral collision are the most at risk of BAT.
Abstract: As the Computed Tomography(CT) requires normally
hundreds of projections to reconstruct the image, patients are exposed
to more X-ray energy, which may cause side effects such as cancer.
Even when the variability of the particles in the object is very less,
Computed Tomography requires many projections for good quality
reconstruction. In this paper, less variability of the particles in an
object has been exploited to obtain good quality reconstruction.
Though the reconstructed image and the original image have same
projections, in general, they need not be the same. In addition
to projections, if a priori information about the image is known,
it is possible to obtain good quality reconstructed image. In this
paper, it has been shown by experimental results why conventional
algorithms fail to reconstruct from a few projections, and an efficient
polynomial time algorithm has been given to reconstruct a bi-level
image from its projections along row and column, and a known sub
image of unknown image with smoothness constraints by reducing the
reconstruction problem to integral max flow problem. This paper also
discusses the necessary and sufficient conditions for uniqueness and
extension of 2D-bi-level image reconstruction to 3D-bi-level image
reconstruction.
Abstract: Computed tomography and laminography are heavily investigated in a compressive sensing based image reconstruction framework to reduce the dose to the patients as well as to the radiosensitive devices such as multilayer microelectronic circuit boards. Nowadays researchers are actively working on optimizing the compressive sensing based iterative image reconstruction algorithm to obtain better quality images. However, the effects of the sampled data’s properties on reconstructed the image’s quality, particularly in an insufficient sampled data conditions have not been explored in computed laminography. In this paper, we investigated the effects of two data properties i.e. sampling density and data incoherence on the reconstructed image obtained by conventional computed laminography and a recently proposed method called spherical sinusoidal scanning scheme. We have found that in a compressive sensing based image reconstruction framework, the image quality mainly depends upon the data incoherence when the data is uniformly sampled.
Abstract: Recent medical studies have investigated the importance of enteral feeding and the use of feeding pumps for recovering patients unable to feed themselves or gain nourishment and nutrients by natural means. The most of enteral feeding system uses a peristaltic tube pump. A peristaltic pump is a form of positive displacement pump in which a flexible tube is progressively squeezed externally to allow the resulting enclosed pillow of fluid to progress along it. The squeezing of the tube requires a precise and robust controller of the geared motor to overcome parametric uncertainty of the pumping system which generates due to a wide variation of friction and slip between tube and roller. So, this paper proposes fuzzy adaptive controller for the robust control of the peristaltic tube pump. This new adaptive controller uses a fuzzy multi-layered architecture which has several independent fuzzy controllers in parallel, each with different robust stability area. Out of several independent fuzzy controllers, the most suited one is selected by a system identifier which observes variations in the controlled system parameter. This paper proposes a design procedure which can be carried out mathematically and systematically from the model of a controlled system. Finally, the good control performance, accurate dose rate and robust system stability, of the developed feeding pump is confirmed through experimental and clinic testing.
Abstract: It is crucial to quantitatively evaluate the treatment of
epilepsy patients. This study was undertaken to test the hypothesis that
compared to the healthy control subjects, the epilepsy patients have
abnormal resting-state connectivity. In this study, we used the
imaginary part of coherency to measure the resting-state connectivity.
The analysis results shown that compared to the healthy control
subjects, epilepsy patients tend to have abnormal rhythm brain
connectivity over their epileptic focus.
Abstract: The paper proposes a methodology to process the signals coming from the Transcranial Magnetic Stimulation (TMS) in order to identify the pathology and evaluate the therapy to treat the patients affected by demency diseases. In particular, a fuzzy model is developed to identify the demency of the patients affected by Subcortical Ischemic Vascular Dementia (SIVD) and to measure the effect of a repetitive TMS on their motor performances. A tool is also presented to support the mentioned analysis.
Abstract: Hearing impairment is the number one chronic
disability affecting many people in the world. Background noise is
particularly damaging to speech intelligibility for people with
hearing loss especially for sensorineural loss patients. Several
investigations on speech intelligibility have demonstrated
sensorineural loss patients need 5-15 dB higher SNR than the normal
hearing subjects. This paper describes Discrete Hartley Transform
Power Normalized Least Mean Square algorithm (DHT-LMS) to
improve the SNR and to reduce the convergence rate of the Least
Means Square (LMS) for sensorineural loss patients. The DHT
transforms n real numbers to n real numbers, and has the convenient
property of being its own inverse. It can be effectively used for noise
cancellation with less convergence time. The simulated result shows
the superior characteristics by improving the SNR at least 9 dB for
input SNR with zero dB and faster convergence rate (eigenvalue ratio
12) compare to time domain method and DFT-LMS.
Abstract: The aim of this study was to estimate the frequency of
EBV infection in Hodgkin's lymphoma (HL) and non-Hodgkin's
lymphoma (NHL) occurring in Jordanian patients. A total of 55
patients with lymphoma were examined in this study. Of 55 patients,
30 and 25 were diagnosed as HL and NHL, respectively. The four
HL subtypes were observed with the majority of the cases exhibited
the mixed cellularity (MC) subtype followed by the nodular sclerosis
(NS). The high grade was found to be the commonest subtype of
NHL in our sample, followed by the low grade. The presence of EBV
virus was detected by immunostating for expression of latent
membrane protein-1 (LMP-1). The frequency of LMP-1 expression
occurred more frequent in patients with HL (60.0%) than in patients
with NHL (32.0%). The frequency of LMP-1 expression was also
higher in patients with MC subtype (61.11%) than those patients with
NS (28.57%). No age or gender difference in occurrence of EBV
infection was observed among patient with HL. By contrast, the
prevalence of EBV infection in NHL patients aged below 50 was
lower (16.66%) than in NHL patients aged 50 or above (46.15%). In
addition, EBV infection was more frequent in females with NHL
(38.46%) than in male with NHL (25%). In NHL cases, the
frequency of EBV infection in intermediate grade (60.0%) was high
when compared with frequency of low (25%) or high grades (25%).
In conclusion, analysis of LMP-1 expression indicates an important
role for this viral oncogene in the pathogenesis of EBV-associated
malignant lymphomas. These data also support the previous findings
that people with EBV may develop lymphoma and that efforts to
maintain low lymphoma should be considered for people with EBV
infection.
Abstract: To distinguish small retinal hemorrhages in early
diabetic retinopathy from dust artifacts, we analyzed hue, lightness,
and saturation (HLS) color spaces. The fundus of 5 patients with
diabetic retinopathy was photographed. For the initial experiment, we
placed 4 different colored papers on the ceiling of a darkroom. Using
each color, 10 fragments of house dust particles on a magnifier were
photographed. The colored papers were removed, and 3 different
colored light bulbs were suspended from the ceiling. Ten fragments of
house dust particles on the camera-s object lens were photographed.
We then constructed an experimental device that can photograph
artificial eyes. Five fragments of house dust particles under the ocher
fundus of the artificial eye were photographed. On analyzing HLS
color space of the dust artifact, lightness and saturation were found to
be highly sensitive. However, hue was not highly sensitive.
Abstract: The nature, prevalence, cellular composition of
leukocyte infiltrates and immunohistochemical characteristics of
their constituent cells in the liver of patients with chronic viral
hepatitis B and C were investigated. It was found that the area of
distribution and cellular composition of infiltrates depended on the
virus type and process activity. The expediency of
immunohistochemical study using leukocyte infiltrates from liver
biopsies of patients with viral hepatitis aimed at clarifying diagnosis,
making prognosis, and choice of optimal treatment with elements of
immune correction is emphasized.
Abstract: Dilated cardiomyopathy (DCM) is a severe
cardiovascular disorder characterized by progressive systolic
dysfunction due to cardiac chamber dilatation and inefficient
myocardial contractility often leading to chronic heart failure.
Recently, a genome-wide association studies (GWASs) on DCM
indicate that the ZBTB17 gene rs10927875 single nucleotide
polymorphism is associated with DCM. The aim of the study was to
identify the distribution of ZBTB17 gene rs10927875 polymorphism
in 50 Slovak patients with DCM and 80 healthy control subjects
using the Custom Taqman®SNP Genotyping assays. Risk factors
detected at baseline in each group included age, sex, body mass
index, smoking status, diabetes and blood pressure. The mean age of
patients with DCM was 52.9±6.3 years; the mean age of individuals
in control group was 50.3±8.9 years. The distribution of investigated
genotypes of rs10927875 polymorphism within ZBTB17 gene in the
cohort of Slovak patients with DCM was as follows: CC (38.8%), CT
(55.1%), TT (6.1%), in controls: CC (43.8%), CT (51.2%), TT
(5.0%). The risk allele T was more common among the patients with
dilated cardiomyopathy than in normal controls (33.7% versus
30.6%). The differences in genotype or allele frequencies of ZBTB17
gene rs10927875 polymorphism were not statistically significant
(p=0.6908; p=0.6098). The results of this study suggest that ZBTB17
gene rs10927875 polymorphism may be a risk factor for
susceptibility to DCM in Slovak patients with DCM. Studies of
numerous files and additional functional investigations are needed to
fully understand the roles of genetic associations.