Abstract: Education, as the most important resource in any country, has multiplying effects on all facets of development in a society. The new social realities, particularly the interplay between democratization of education; unprecedented developments in IT sector; emergence of knowledge society, liberalization of economy and globalization have greatly influenced the educational process of all nations. This turbulence entails upon education to undergo dramatic changes to keep up with the new expectations. Growth of entrepreneurship among Indian women is highly important for empowering them and this is highly essential for socio-economic development of a society. Unfortunately in India there is poor acceptance of entrepreneurship among women as unfounded myths and fears restrain them to be enterprising. To remove these inhibitions, education system needs to be re-engineered to make entrepreneurship more acceptable. This paper empirically analyses the results of a survey done on around 500 female graduates in North India to measure and evaluate various entrepreneurial traits present in them. A formative model has been devised in this context, which should improve the teaching-learning process in our education system, which can lead to sustainable growth of women entrepreneurship in India.
Abstract: Phytotoxicity of Daphne gnidium L. was evaluated
through the effect of incorporating leaves, stems and roots biomass
into soil (at 12.5, 25, 50g/Kg) and irrigation by their aqueous extracts
(50g/L), on the growth of two crops (Lactuca sativa L. and Raphanus
sativus L.) and two weeds (Peaganum harmala L. and Scolymus
maculatus L.). Results revealed a perceptible phytotoxic effect which
increased with dose and concentration. At the highest dose, roots and
leaves residues was the most toxic and caused total inhibition
respectively, for lettuce and thistle seedling growth. Irrigation with
aqueous extracts of D. gnidium different organs decreased also
seedlings length of all test species. Stems extract was more inhibitor
on thistle than peganum seedling growth; it induced a significant
reduction of 80% and 67%, for, respectively, roots and shoots.
Results of the present study suggest that different organs of D.
gnidium could be exploited in the management of agro-ecosystems.
Abstract: The association between emotional inhibition strategies linked to depression has been showed inconsistent among studies. Mild emotional inhibition maybe benefit for social interaction, especially for female among East Asian cultures. The present study aimed to examine whether the inhibition–depression relationship is dependent on level of emotion inhibition and gender context, given differing value of suppressing emotional displays. We hypothesized that the negative associations between inhibition and adolescent depression would not directly, in which affected by interaction between emotion inhibition and gender. To test this hypothesis, we asked 309 junior high school students which age range from 12 to14 years old to report on their use of emotion inhibition and depression syndrome. A multiple regressions analysis revealed that significant interaction that gender as a moderator to the relationships between emotion inhibition and adolescent depression. The group with the highest level of depression was girls with high levels of emotion inhibition, whose depression score was higher than that of boys with high levels of emotion inhibition. The result highlights that the importance of context in understanding the inhibition-depression relationship.
Abstract: This paper explains how mobile learning assures sustainable e-education for multicultural group of students. This paper reports the impact of mobile learning on distance education in multicultural environment. The emergence of learning technologies through CD, internet, and mobile is increasingly adopted by distance institutes for quick delivery and cost-effective purposes. Their sustainability is conditioned by the structure of learners as well as the teaching community. The experimental study was conducted among the distant learners of Vinayaka Missions University located at Salem in India. Students were drawn from multicultural environment based on different languages, religions, class and communities. During the mobile learning sessions, the students, who are divided on language, religion, class and community, were dominated by play impulse rather than study anxiety or cultural inhibitions. This study confirmed that mobile learning improved the performance of the students despite their division based on region, language or culture. In other words, technology was able to transcend the relative deprivation in the multicultural groups. It also confirms sustainable e-education through mobile learning and cost-effective system of instruction. Mobile learning appropriates the self-motivation and play impulse of the young learners in providing sustainable e-education to multicultural social groups of students.
Abstract: Vernonia divergens Benth., commonly known as
“Insulin Plant” (Fam: Asteraceae) is a potent sugar killer. Locally the
leaves of the plant, boiled in water are successfully administered to a
large number of diabetic patients. The present study evaluates the
putative anti-diabetic ingredients, isolated from the in vivo and in
vitro grown plantlets of V. divergens for their antimicrobial and
anticancer activities. Sterilized explants of nodal segments were
cultured on MS (Musashige and Skoog, 1962) medium in presence of
different combinations of hormones. Multiple shoots along with
bunch of roots were regenerated at 1mg l-1 BAP and 0.5 mg l-1 NAA.
Micro-plantlets were separated and sub-cultured on the double
strength (2X) of the above combination of hormones leading to
increased length of roots and shoots. These plantlets were
successfully transferred to soil and survived well in nature. The
ethanol extract of plantlets from both in vivo & in vitro sources were
prepared in soxhlet extractor and then concentrated to dryness under
reduced pressure in rotary evaporator. Thus obtainedconcentrated
extracts showed significant inhibitory activity against gram
negative bacteria like Escherichia coli and Pseudomonas
aeruginosa but no inhibition was found against gram positive
bacteria. Further, these ethanol extracts were screened for in vitro
percentage cytotoxicity at different time periods (24 h, 48 h and 72 h)
of different dilutions. The in vivo plant extract inhibited the growth of
EAC mouse cell lines in the range of 65, 66, 78, and 88% at 100, 50,
25 & 12.5μg mL-1 but at 72 h of treatment. In case of the extract of in
vitro origin, the inhibition was found against EAC cell lines even at
48h. During spectrophotometric scanning, the extracts exhibited
different maxima (ʎ) - four peaks in in vitro extracts as against single
in in vivo preparation suggesting the possible change in the nature of
ingredients during micropropagation through tissue culture
techniques.
Abstract: In this study, cometabolic biodegradation of
chloroform was experimented with mixed cultures in the presence of
various organic solvents like methanol, ethanol, isopropanol, acetone,
acetonitrile and toluene as these are predominant discharges in
pharmaceutical industries. Toluene and acetone showed higher
specific chloroform degradation rate when compared to other
compounds. Cometabolic degradation of chloroform was further
confirmed by observation of free chloride ions in the medium. An
extended Haldane model, incorporating the inhibition due to
chloroform and the competitive inhibition between primary
substrates, was developed to predict the biodegradation of primary
substrates, cometabolic degradation of chloroform and the biomass
growth. The proposed model is based on the use of biokinetic
parameters obtained from single substrate degradation studies. The
model was able to satisfactorily predict the experimental results of
ternary and quaternary mixtures. The proposed model can be used for
predicting the performance of bioreactors treating discharges from
pharmaceutical industries.
Abstract: 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) catalyzes the conversion of HMG-CoA to mevalonate using NADPH and the enzyme is involved in rate-controlling step of mevalonate. Inhibition of HMGR is considered as effective way to lower cholesterol levels so it is drug target to treat hypercholesterolemia, major risk factor of cardiovascular disease. To discover novel HMGR inhibitor, we performed structure-based pharmacophore modeling combined with molecular dynamics (MD) simulation. Four HMGR inhibitors were used for MD simulation and representative structure of each simulation were selected by clustering analysis. Four structure-based pharmacophore models were generated using the representative structure. The generated models were validated used in virtual screening to find novel scaffolds for inhibiting HMGR. The screened compounds were filtered by applying drug-like properties and used in molecular docking. Finally, four hit compounds were obtained and these complexes were refined using energy minimization. These compounds might be potential leads to design novel HMGR inhibitor.
Abstract: In this study, three subtypes of influenza A viruses (pH1N1, H5N1 and H3N2) which naturally infected human were analyzed by bioinformatic approaches to find candidate human cellular miRNAs targeting viral genomes. There were 76 miRNAs targeting influenza A viruses. Among these candidates, 70 miRNAs were subtypes specifically targeting each subtype of influenza A virus including 21 miRNAs targeted subtype H1N1, 27 miRNAs targeted subtype H5N1 and 22 miRNAs targeted subtype H3N2. The remaining 6 miRNAs target on multiple subtypes of influenza A viruses. Uniquely, hsa-miR-3145 is the only one candidate miRNA targeting PB1 gene of all three subtypes. Obviously, most of the candidate miRNAs are targeting on polymerase complex genes (PB2, PB1 and PA) of influenza A viruses. This study predicted potential human miRNAs targeting on different subtypes of influenza A viruses which might be useful for inhibition of viral replication and for better understanding of the interaction between virus and host cell.
Abstract: In the following text, we show that by introducing
universal kinetic scheme, the origin of rate retardation and inhibition
period which observed in dithiobenzoate-mediated RAFT
polymerization can be described properly. We develop our model by
utilizing the method of moments, then we apply our model to
different monomer/RAFT agent systems, both homo- and
copolymerization. The modeling results are in an excellent
agreement with experiments and imply the validity of universal
kinetic scheme, not only for dithiobenzoate-mediated systems, but
also for different types of monomer/RAFT agent ones.
Abstract: EcoDam is an adenine-N6 DNA methyltransferase
that methylates the GATC sites in the Escherichia coli genome.
DNA-adenine methylation is not present in higher eukaryotes
including humans. These observations raise the possibility that dam
inhibitors may be used as anti-microbial agents. Polyphosphate
(Poly(P)) is an important metabolite and signaling molecule in
prokaryotes and eukaryotes. Here, by using gel retardation
experiments to investigate the competition of DNA binding by
EcoDam in the presence of polyphosphate, we found that Poly (P)
strongly interferes with DNA binding by EcoDam, while same
concentration of monophosphate does not. In addition, we
demonstrated that Poly (P) binding inhibits the activity of EcoDam
and our results suggest that Poly (P) led to strong inhibition of the
EcoDam catalytic activity, while monophosphate had only moderate
effect.
Abstract: Antibacterial activity of Plumeria alba (Frangipani)
petals methanolic extracts were evaluated against Escherichia coli,
Proteus vulgaris,Staphylococcus aureus, Klebsiella pneumoniae,
Pseudomonas aeruginosa, Staphylococcus saprophyticus,
Enterococcus faecalis and Serratia marcescens by using disk
diffusion method. Concentration extracts (80 %) showed the highest
inhibition zone towards Escherichia coli (14.3 mm). Frangipani
extract also showed high antibacterial activity against
Staphylococcus saprophyticus, Proteus vulgaris and Serratia
marcescens, but not more than the zones of the positive control used.
Comparison between two broad specrum antibiotics to frangipani
extracts showed that the 80 % concentration extracts produce the
same zone of inhibition as Streptomycin. Frangipani extracts showed
no bacterial activity towards Klebsiella pneumoniae, Pseudomonas
aeruginosa and Enterococcus faecalis. There are differences in the
sensitivity of different bacteria to frangipani extracts, suggesting that
frangipani-s potency varies between these bacteria. The present
results indicate that frangipani showed significant antibacterial
activity especially to Escherichia coli.
Abstract: In production of medicinal plants, seed germination is
very important problem. The treated seeds (control, hydro priming
and ZnSO4) of Cumin (Cuminum cyminum L.) were evaluated at
germination and seedling growth for tolerance to salt (NaCl and
Na2SO4) conditions at the same water potentials of 0.0, -0.3, -0.6, -
0.9 and -1.2MPa. Electrical conductivity (EC) values of the NaCl
solutions were 0.0, 6.5, 12.7, 18.4 and 23.5 dSm-1, respectively. The
objective of the study was to determine factors responsible for
germination and early seedling growth due to salt toxicity or osmotic
effect and to optimize the best priming treatment for these stress
conditions. Results revealed that germination delayed in both
solutions, having variable germination with different priming
treatments. Germination, shoot and weight, root and shoot length
were higher but mean germination time and abnormal germination
percentage were lower in NaCl than Na2SO4 at the same water
potential. The root / shoot weight and R/S length increased with
increase in osmotic potential in both NaCl and Na2SO4 solutions.
NaCl had less inhibitor effect on seedling growth than the
germination. It was concluded that inhibition of germination at the
same water potential of NaCl and Na2SO4 resulted from salt toxicity
rather than osmotic effect. Hydro priming increased germination and
seedling growth under salt stress. This protocol has practical
importance and could be recommended to farmers to achieve higher
germination and uniform emergence under field conditions.
Abstract: Pentachlorophenol (PCP) is a polychlorinated
aromatic compound that is widespread in industrial effluents and is
considered to be a serious pollutant. Among the variety of industrial
effluents encountered, effluents from tanning industry are very
important and have a serious pollution potential. PCP is also formed
unintentionally in effluents of paper and pulp industries. It is highly
persistent in soils and is lethal to a wide variety of beneficial
microorganisms and insects, human beings and animals. The natural
processes that breakdown toxic chemicals in the environment have
become the focus of much attention to develop safe and environmentfriendly
deactivation technologies. Microbes and plants are among
the most important biological agents that remove and degrade waste
materials to enable their recycling in the environment. The present
investigation was carried out with the aim of developing a microbial
system for bioremediation of PCP polluted soils. A number of plant
species were evaluated for their ability to tolerate different
concentrations of pentachlorophenol (PCP) in the soil. The
experiment was conducted for 30 days under pot culture conditions.
The toxic effect of PCP on plants was studied by monitoring seed
germination, plant growth and biomass. As the concentration of PCP
was increased to 50 ppm, the inhibition of seed germination, plant
growth and biomass was also increased. Although PCP had a
negative effect on all plant species tested, maize and groundnut
showed the maximum tolerance to PCP. Other tolerating crops
included wheat, safflower, sunflower, and soybean. From the
rhizosphere soil of the tolerant seedlings, as many as twenty seven
PCP tolerant bacteria were isolated. From soybean, 8; sunflower, 3;
safflower 8; maize 2; groundnut and wheat, 3 each isolates were
made. They were screened for their PCP degradation potentials.
HPLC analyses of PCP degradation revealed that the isolate MAZ-2
degraded PCP completely. The isolate MAZ-1 was the next best
isolate with 90 per cent PCP degradation. These strains hold promise
to be used in the bioremediation of PCP polluted soils.
Abstract: Characterization and evaluation of the activity of Vespa basalis DPP-IV, which expressed in Spodoptera frugiperda 21 cells. The expression of rDPP-IV was confirmed by SDS–PAGE, Western blot analyses, LC-MS/MS and measurement of its peptidase specificity. One-step purification by Ni-NTA affinity chromatography and the total amount of rDPP-IV recovered was approximately 6.4mg per liter from infected culture medium; an equivalent amount would be produced by 1x109 infected Sf21 insect cells. Through the affinity purification led to highly stable rDPP-IV enzyme was recovered and with significant peptidase activity. The rDPP-IV exhibited classical Michaelis–Menten kinetics, with kcat/Km in the range of 10-500 mM-1×S-1 for the five synthetic substrates and optimum substrate is Ala-Pro-pNA. As expected in inhibition assay, the enzymatic activity of rDPP-IV was significantly reduced by 80 or 60% in the presence of sitagliptin (a DPP-IV inhibitor) or PMSF (a serine protease inhibitor), but was not apparently affected by iodoacetamide (a cysteine protease inhibitor).
Abstract: In order to enhance the knowledge of certain
phytochemical Algerian plants that are widely used in traditional
medicine and to exploit their therapeutic potential in modern
medicine, we have done a specific extraction of terpenes and
alkaloids from the leaves of Euphorbia granulata to evaluate the
antioxidant and antibacterial activity of this extracts. After the
extraction it was found that the terpene extract gave the highest yield
59.72% compared with alkaloids extracts.
The disc diffusion method was used to determine the antibacterial
activity against different bacterial strains: Escherichia coli
(ATCC25922), Pseudomonas aeruginosa (ATCC27853) and
Staphylococcus aureus (ATCC25923). All extracts have shown
inhibition of growth bacteria. The different zones of inhibition have
varied from (7 -10 mm) according to the concentrations of extract
used.
Testing the antiradical activity on DPPH-TLC plates indicated the
presence of substances that have potent anti-free radical. As against,
the BC-TLC revealed that only terpenes extract which was reacted
positively. These results can validate the importance of Euphorbia
granulata in traditional medicine.
Abstract: The bioassay-guided isolation and purification of an
ethyl acetate extract of Aspergillus terreus MC751 led to the
characterization of butyrolactone I as an antidiabetic and antioxidant.
The antidiabetic activity of butyrolactone I was evaluated by α-
glucosidase and α-amylase inhibition assays. Butyrolactone I
demonstrated significant concentration-dependent, mixed-type
inhibitory activity against yeast α-glucosidase with an IC50 of 54μM.
However, the compound exhibited less activity against rat intestinal
α-glucosidase and α-amylase. This is the first report on α-glucosidase
inhibitory activity of butyrolactone I. The antioxidative activity of
butyrolactone I was evaluated based on scavenging effects on 1,1-
diphenyl-2-picrylhydrazyl (DPPH) (IC50 =51 μM) and hydrogen
peroxide (IC50= 141 μM) radicals as well as a reducing power assay.
The results suggest that butyrolactone I is a promising antidiabetic as
well as antioxidant and should be considered for clinical trials.
Abstract: NFκB activation plays a crucial role in anti-apoptotic responses in response to the apoptotic signaling during tumor necrosis factor (TNFa) stimulation in Multiple Myeloma (MM). Although several drugs have been found effective for the treatment of MM by mainly inhibiting NFκB pathway, there are no any quantitative or qualitative results of comparison assessment on inhibition effect between different single drugs or drug combinations. Computational modeling is becoming increasingly indispensable for applied biological research mainly because it can provide strong quantitative predicting power. In this study, a novel computational pathway modeling approach is employed to comparably assess the inhibition effects of specific single drugs and drug combinations on the NFκB pathway in MM, especially the prediction of synergistic drug combinations.
Abstract: Candida spp. are common and aggressive pathogens.
Because of the growing resistance of Candida spp. to current
antifungals, novel targets, found in Candida spp. but not in humans
or other flora, have to be identified. The alternative oxidase (AOX)
is one such possibility. This enzyme is insensitive to cyanide, but is
sensitive to compounds such as salicylhydroxamic acid (SHAM),
disulfiram and n-alkyl gallates. The growth Candida albicans was
inhibited by SHAM (Ki = 9-15 mM) and cyanide (Ki = 2-4 mM),
albeit to differing extents. The rate of O2 uptake was inhibited by
less than 10% by 25 mM SHAM and by about 90% by 250 μM
KCN. Although SHAM substantially inhibited the growth of C.
albicans, it is unlikely that the inhibition of AOX was the cause.
Salicylhydroxamic acid is used therapeutically in the treatment of
urinary tract infections and urolithiasis, but it also has some potential
in the treatment of C. albicans infection.
Abstract: In this study, inhibition of Microcystis aeruginosa by
antialgal alleochemical gramine, was studied by analyzing algal
metabolic activity (represented by esterase and total dehydrogenase
activities) and cell ultrastructure (showing morphological and
ultrastructure alterations using transmission electron microscopy and
DNA ladder analysis). After gramine exposure, esterase and total
dehydrogenase activities were increased firstly but decreased later. In
contrast with the controls, the cells exposed to gramine showed
apparent ultrastructure alterations with thylakoids in breakage,
phycobilins in decrease, lipid and cyanophycin granules abundant
firstly but dissolved afterwards, DNA in fragementation. The
occurrence of increase of metabolic activity and specific granules
reflected that the resistance of cellular response to gramine was
initiated. DNA fragementation associated with the increase of
metabolic activity and specific granules hinted that gramine caused M.
aeruginosa cells to initiate some morphotype of programmed cell
death.
Abstract: Proteins levels produced by bacteria may be increased
in stressful surroundings, such as in the presence of antibiotics. It
appears that many antimicrobial agents or antibiotics, when used at
low concentrations, have in common the ability to activate or repress
gene transcription, which is distinct from their inhibitory effect.
There have been comparatively few studies on the potential of
antibiotics or natural compounds in nature as a specific chemical
signal that can trigger a variety of biological functions. Therefore,
this study was focusing on the effect of essential oils from
Cymbopogon flexuosus and C. nardus in regulating proteins
production by Bacillus subtilis ATCC 21332. The Minimum
Inhibition Concentrations (MICs) of both essential oils on B. subtilis
were determined by using microdilution assay, resulting 0.2% and
1.56% for each C. flexuosus and C. nardus subsequently. The
bacteria were further exposed to each essential oils at concentration
of 0.01XMIC for 2 days. The proteins were then isolated and
analyzed by sodium dodecyl sulfate polyacrylamide gel
electrophoresis (SDS-PAGE). Protein profile showed that a band
with approximate size of 250 kD was appeared for the treated
bacteria with essential oils. Thus, Bacillus subtilis ATCC 21332 in
stressful condition with the presence of essential oils at low
concentration could induce the protein production.