Abstract: Type 2 diabetes mellitus (T2DM) is a complex
metabolic disorder that characterized by the presence of high glucose
in blood that cause from insulin resistance and insufficiency due to
deterioration β-cell Langerhans functions. T2DM is commonly
caused by the combination of inherited genetic variations as well as
our own lifestyle. Metallothionein (MT) is a known cysteine-rich
protein responsible in helping zinc homeostasis which is important in
insulin signaling and secretion as well as protection our body from
reactive oxygen species (ROS). MT scavenged ROS and free
radicals in our body happen to be one of the reasons of T2DM and its
complications. The objective of this study was to investigate the
association of MT1A and MT2A polymorphisms between T2DM and
control subjects among Malay populations. This study involved 150
T2DM and 120 Healthy individuals of Malay ethnic with mixed
genders. The genomic DNA was extracted from buccal cells and
amplified for MT1A and MT2A loci; the 347bp and 238bp banding
patterns were respectively produced by mean of the Polymerase
Chain Reaction (PCR). The PCR products were digested with Mlucl
and Tsp451 restriction enzymes respectively and producing
fragments lengths of (158/189/347bp) and (103/135/238bp)
respectively. The ANOVA test was conducted and it shown that there
was a significant difference between diabetic and control subjects for
age, BMI, WHR, SBP, FPG, HBA1C, LDL, TG, TC and family
history with (P0.05). The genotype
frequency for AA, AG and GG of MT1A polymorphisms was 72.7%,
22.7% and 4.7% in cases and 15%, 55% and 30% in control
respectively. As for MT2A, genotype frequency of GG, GC and CC
was 42.7%, 27.3% and 30% in case and 5%, 40% and 55% for
control respectively. Both polymorphisms show significant difference
between two investigated groups with (P=0.000). The Post hoc test
was conducted and shows a significant difference between the
genotypes within each polymorphism (P=0. 000). The MT1A and
MT2A polymorphisms were believed to be the reliable molecular
markers to distinguish the T2DM subjects from healthy individuals in
Malay populations.
Abstract: To evaluate genetic variation of wheat (Triticum
aestivum) affected by heat and drought stress on eight Australian
wheat genotypes that are parents of Doubled Haploid (HD) mapping
populations at the vegetative stage, the water stress experiment was
conducted at 65% field capacity in growth room. Heat stress
experiment was conducted in the research field under irrigation over
summer. Result show that water stress decreased dry shoot weight
and RWC but increased osmolarity and means of Fv/Fm values in all
varieties except for Krichauff. Krichauff and Kukri had the
maximum RWC under drought stress. Trident variety was shown
maximum WUE, osmolarity (610 mM/Kg), dry mater, quantum yield
and Fv/Fm 0.815 under water stress condition. However, the
recovery of quantum yield was apparent between 4 to 7 days after
stress in all varieties. Nevertheless, increase in water stress after that
lead to strong decrease in quantum yield. There was a genetic
variation for leaf pigments content among varieties under heat stress.
Heat stress decreased significantly the total chlorophyll content that
measured by SPAD. Krichauff had maximum value of Anthocyanin
content (2.978 A/g FW), chlorophyll a+b (2.001 mg/g FW) and
chlorophyll a (1.502 mg/g FW). Maximum value of chlorophyll b
(0.515 mg/g FW) and Carotenoids (0.234 mg/g FW) content
belonged to Kukri. The quantum yield of all varieties decreased
significantly, when the weather temperature increased from 28 ÔùªC to
36 ÔùªC during the 6 days. However, the recovery of quantum yield
was apparent after 8th day in all varieties. The maximum decrease
and recovery in quantum yield was observed in Krichauff. Drought
and heat tolerant and moderately tolerant wheat genotypes were
included Trident, Krichauff, Kukri and RAC875. Molineux, Berkut
and Excalibur were clustered into most sensitive and moderately
sensitive genotypes. Finally, the results show that there was a
significantly genetic variation among the eight varieties that were
studied under heat and water stress.
Abstract: It is believed that DNA damaging toxic metabolites contributes to the development of different pathological conditions. To prevent harmful influence of toxic agents, cells developed number of protecting mechanisms, such as enzymatic reaction of detoxification of reactive metabolites and repair of DNA damage. The aim of the study was to examine the association between polymorphism of GSTT1/GSTM1 and XRCC1/3 genes and coronary artery disease (CAD) incidence. To examine a polymorphism of these genes in CAD susceptibility in patients and controls, PCR based genotyping assay was performed. For GST genes, frequency of GSTM1 null genotype among CAD affected group was significantly increased than in control group (P0.1). We found that neither XRCC1 Arg399Gln nor XRCC3 Thr241Met were associated with CAD risk. Obtained data suggests that GSTM1 null genotype carriers are more susceptible to CAD development.
Abstract: Data on 657 lactation from 163 Maltese goat,
collected over a 5-year period were analyzed by a mixed model to
estimate the variance components for heritability. The considered
lactation traits were: milk yield (MY) and lactation length (LL). Year,
parity and type of birth (single or twin) were significant sources of
variation for lactation length; on the other hand milk yield was
significantly influenced only by the year. The average MY was
352.34 kg and the average LL was 230 days. Estimates of heritability
were 0.21 and 0.15 for MY and LL respectively. These values
suggest there is low correlation between genotype and phenotype so
it may be difficult to evaluate animals directly on phenotype. So, the
genetic improvement of this breed may be quite slow without the
support of progeny test aimed to select Maltese breeders.
Abstract: As part of national epidemiological survey on bovine
viral diarrhea virus (BVDV), a total of 274 dejecta samples were
collected from 14 cattle farms in 8 areas of Xinjiang Uygur
Autonomous Region in northwestern China. Total RNA was extracted
from each sample, and 5--untranslated region (UTR) of BVDV
genome was amplified by using two-step reverse
transcriptase-polymerase chain reaction (RT-PCR). The PCR products
were subsequently sequenced to study the genetic variations of BVDV
in these areas. Among the 274 samples, 33 samples were found
virus-positive. According to sequence analysis of the PCR products,
the 33 samples could be arranged into 16 groups. All the sequences,
however, were highly conserved with BVDV Osloss strains. The virus
possessed theses sequences belonged to BVDV-1b subtype by
phylogenetic analysis. Based on these data, we established a typing
tree for BVDV in these areas. Our results suggested that BVDV-1b
was a predominant subgenotype in northwestern China and no
correlation between the genetic and geographical distances could be
observed above the farm level.
Abstract: The p53 tumor suppressor gene plays two important
roles in genomic stability: blocking cell proliferation after DNA
damage until it has been repaired, and starting apoptosis if the
damage is too critical. Codon 72 exon4 polymorphism (Arg72Pro) of
the P53 gene has been implicated in cancer risk. Various studies have
been done to investigate the status of p53 at codon 72 for arginine
(Arg) and proline (Pro) alleles in different populations and also the
association of this codon 72 polymorphism with various tumors. Our
objective was to investigate the possible association between P53
Arg72Pro polymorphism and susceptibility to colorectal cancer
among Isfahan and Chaharmahal Va Bakhtiari (a part of south west
of Iran) population. We investigated the status of p53 at codon 72 for
Arg/Arg, Arg/Pro and Pro/Pro allele polymorphisms in blood
samples from 145 colorectal cancer patients and 140 controls by
Nested-PCR of p53 exon 4 and digestion with BstUI restriction
enzyme and the DNA fragments were then resolved by
electrophoresis in 2% agarose gel. The Pro allele was 279 bp, while
the Arg allele was restricted into two fragments of 160 and 119 bp.
Among the 145 colorectal cancer cases 49 cases (33.79%) were
homozygous for the Arg72 allele (Arg/Arg), 18 cases (12.41%) were
homozygous for the Pro72 allele (Pro/Pro) and 78 cases (53.8%)
found in heterozygous (Arg/Pro).
In conclusion, it can be said that p53Arg/Arg genotype may be
correlated with possible increased risk of this kind of cancers in south
west of Iran.
Abstract: In the recent past Learning Classifier Systems have
been successfully used for data mining. Learning Classifier System
(LCS) is basically a machine learning technique which combines
evolutionary computing, reinforcement learning, supervised or
unsupervised learning and heuristics to produce adaptive systems. A
LCS learns by interacting with an environment from which it
receives feedback in the form of numerical reward. Learning is
achieved by trying to maximize the amount of reward received. All
LCSs models more or less, comprise four main components; a finite
population of condition–action rules, called classifiers; the
performance component, which governs the interaction with the
environment; the credit assignment component, which distributes the
reward received from the environment to the classifiers accountable
for the rewards obtained; the discovery component, which is
responsible for discovering better rules and improving existing ones
through a genetic algorithm. The concatenate of the production rules
in the LCS form the genotype, and therefore the GA should operate
on a population of classifier systems. This approach is known as the
'Pittsburgh' Classifier Systems. Other LCS that perform their GA at
the rule level within a population are known as 'Mitchigan' Classifier
Systems. The most predominant representation of the discovered
knowledge is the standard production rules (PRs) in the form of IF P
THEN D. The PRs, however, are unable to handle exceptions and do
not exhibit variable precision. The Censored Production Rules
(CPRs), an extension of PRs, were proposed by Michalski and
Winston that exhibit variable precision and supports an efficient
mechanism for handling exceptions. A CPR is an augmented
production rule of the form: IF P THEN D UNLESS C, where
Censor C is an exception to the rule. Such rules are employed in
situations, in which conditional statement IF P THEN D holds
frequently and the assertion C holds rarely. By using a rule of this
type we are free to ignore the exception conditions, when the
resources needed to establish its presence are tight or there is simply
no information available as to whether it holds or not. Thus, the IF P
THEN D part of CPR expresses important information, while the
UNLESS C part acts only as a switch and changes the polarity of D
to ~D. In this paper Pittsburgh style LCSs approach is used for
automated discovery of CPRs. An appropriate encoding scheme is
suggested to represent a chromosome consisting of fixed size set of
CPRs. Suitable genetic operators are designed for the set of CPRs
and individual CPRs and also appropriate fitness function is proposed
that incorporates basic constraints on CPR. Experimental results are
presented to demonstrate the performance of the proposed learning
classifier system.
Abstract: This study carried out in order to investigate the
effects of salinity on carbon isotope discrimination (Δ) of shoots and
roots of four sugar beet cultivars (cv) including Madison (British
origin) and three Iranian culivars (7233-P12, 7233-P21 and 7233-P29).
Plants were grown in sand culture medium in greenhouse conditions.
Plants irrigated with saline water (tap water as control, 50 mM, 150
mM, 250 mM and 350 mM of NaCl + CaCl2 in 5 to 1 molar ratio)
from 4 leaves stage for 16 weeks. Carbon isotope discrimination
significantly decreased with increasing salinity. Significant
differences of Δ between shoot and root were observed in all cvs and
all levels of salinity. Madison cv showed lower Δ in shoot and root
than other three cvs at all levels of salinity expect control, but cv
7233-P29 had significantly higher Δ values at saline conditions of 150
mM and above. Therefore, Δ might be applicable, as a useful tool, for
study of salinity tolerance of sugar beet genotypes.
Abstract: The objective was to determine the single gene and
interaction effect of composite genotype of beta-kappa casein and
DGAT1 gene on milk yield (MY) and milk composition, content of
milk fat (%FAT), milk protein (%PRO), solid not fat (%SNF), and
total solid (%TS) in crossbred Holstein cows. Two hundred and
thirty- one cows were genotyped with PCR-RFLP for DGAT1 and
composite genotype data of beta-kappa casein from previous work
were used. Two model, (1), and (2), was used to estimate single gene
effect, and interaction effect on the traits, respectively. The
significance of interaction effects on all traits were detected. Most
traits have consistent pattern of significant when model (1), and (2)
were compared, except the effect of composite genotype of betakappa
casein on %FAT, and the effect of DGAT1 on MY, which the
significant difference was detected in only model (1).The results
suggested that when the optimum of all traits was necessary,
interaction effect should be concerned.
Abstract: The influence of humidity and low temperature on the α- amylase activity and isoenzyme composition of grains of different wheat varieties have been studied. The identified samples of varieties have significant difference in the level of enzyme induction under the impact of high humidity and low temperature. It is proposed to use this methodological approach for testing genotypes and wheat breeding lines for resistance to pre-harvest sprouting (PHS).
Abstract: A lot of recent research have spoken on the relation
between the increase of the homocysteinemia and some kinds of
cancer . For that, our study was based on the research of a possible
relation between the increase of the concentration of this amino-acid
in the plasma and the appearance of the disease of the Acute
Lymphoblastic Leukaemia in a part of Algerian children with Berber
origin in the East of Algeria . The study has done on 47 ill persons
with an average age of (09±06 ) years , with whom the disease has
diagnosed by blood and marrow examination in the hospital of blood
diseases in the CHU of Batna, and on 194 healthy witnesses of the
same age. The two groups were benefited by a dosage of the
concentration of the homocysteine vitamin B9 ,vitamin B12 , and
also of the study of special polymorphisms of indispensable enzymes
in the metabolism of this acid , and that by the use of the method (
Light cycler ) Real time PCR , on the following enzymes : MS (
C2756G ), MSR ( A66G ) ,MTHFR1 ( C677T ) and MTHFR2
(A1298C). The obtained results have revealed that the rate of the
homozygote muted genotype is the less frequent in the two groups ,
and that exist at list one genotype of each enzyme in the ill group and
in which the percentage exceed with remarkable way the same
genotype in the healthy group and we notice specially the muted
genotype GG of -the methionine synthetase-and the form TT of the
enzyme – methyline tetra hydrofolate reductase – We notice the
existence of considerable number of genotypes in the ill group lied
with characteristic increase of this Amino-acid ,and that for the
reduction of the biologic activity of these enzymes which become
inefficient in the transfer of the homocysteine into the methionine
and cause the diminution of the biologic activity of these enzymes
and with consequence the reduction of the percentage of methylic
radicals in the DNA of studied genes and that lead to the increase of
the activity and the capacity of transcription , and it-s so probably
that this last one is one of the factors of this disease especially if we
know that the specific check-up of vitamins is normal and similar in
the two groups , which ovoid the hypothesis of the reduction of
vitamins . We notice also that the heterozygote genotype is the less in
the sick category except the MTHFR2. Wild genotype is more
frequent in the witness group except MSR. Even these results are
partials; they open a new way in the genetic diagnosis of this
malicious disease which allow a precocious diagnosis and the use of
an effective and appropriated treatment in the same time.
Abstract: Post-anthesis drought stress is the most important
problem affecting wheat production in dryland fields, specially in
Mediterranean regions. The main objective of this research was to
evaluate drought tolerance indices in dryland wheat genotypes under
post-anthesis drought stress. The research was including two different
experiments. In each experiment, twenty dryland bread wheat
genotypes were sown in a randomized complete blocks design
(RCBD) with three replications. One of experiments belonged to
rain-fed conditions (post-anthesis drought stress) and other
experiment was under non-stress conditions (with supplemental
irrigation). Different drought tolerance indices include Stress
Tolerance (Tol), Mean Productivity (MP), Geometric Mean
Productivity (GMP), Stress Susceptibility Index (SSI), Stress
Tolerance Index (STI), Harmonic Mean (HAM), Yield Index (YI)
and Yield Stability Index (YSI) were evaluate based on grain yield
under rain-fed (Ys) and supplemental irrigation (Yp) environments.
G10 and G12 were the most tolerant genotypes based on TOL and
SSI. But, based on MP, GMP, STI, HAM and YI indices, G1 and G2
were selected. STI, GMP and MP indices had high correlation with
grain yield under rain-fed and supplementary irrigation conditions
and were recognized as appropriate indices to identify genotypes with
high grain yield and low sensitivity to drought stress environments.
Abstract: MMR vaccine failure had been reported globally and
here we report that it occurs now in India. Samples were collected from clinically suspected mumps cases were subjected for anti
mumps antibodies, virus isolation, RT-PCR, sequencing and
phylogenetic tree analysis. 56 samples collected from men and women belonging to various age groups. 30 had been vaccinated and
the status of 26 patients was unknown. 28 out of 30 samples were
found to be symptomatic and positive for Mumps IgM, indicating
active mumps infection in 93.4% of the vaccinated population. A
phylogenetic tree comparison of the clinical isolate is shown to be genotype C which is distinct from vaccine strain. Our study clearly sending warning signs that MMR vaccine is a failure and it needs to be revamped for the human use by increasing its efficacy and efficiency.
Abstract: Evolutionary Programming (EP) represents a
methodology of Evolutionary Algorithms (EA) in which mutation is
considered as a main reproduction operator. This paper presents a
novel EP approach for Artificial Neural Networks (ANN) learning.
The proposed strategy consists of two components: the self-adaptive,
which contains phenotype information and the dynamic, which is
described by genotype. Self-adaptation is achieved by the addition of
a value, called the network weight, which depends on a total number
of hidden layers and an average number of neurons in hidden layers.
The dynamic component changes its value depending on the fitness
of a chromosome, exposed to mutation. Thus, the mutation step size
is controlled by two components, encapsulated in the algorithm,
which adjust it according to the characteristics of a predefined ANN
architecture and the fitness of a particular chromosome. The
comparative analysis of the proposed approach and the classical EP
(Gaussian mutation) showed, that that the significant acceleration of
the evolution process is achieved by using both phenotype and
genotype information in the mutation strategy.
Abstract: The selection of parents and breeding strategies for
the successful maize hybrid production will be facilitated by
heterotic groupings of parental lines and determination of combining
abilities of them. Fourteen maize inbred lines, used in maize breeding
programs in Iran, were crossed in a diallel mating design. The 91 F1
hybrids and the 14 parental lines were studied during two years at
four locations of Iran for investigation of combining ability of
gentypes for grain yield and to determine heterotic patterns among
germplasm sources, using both, the Griffing-s method and the biplot
approach for diallel analysis. The graphical representation offered by
biplot analysis allowed a rapid and effective overview of general
combining ability (GCA) and specific combining ability (SCA)
effects of the inbred lines, their performance in crosses, as well as
grouping patterns of similar genotypes. GCA and SCA effects were
significant for grain yield (GY). Based on significant positive GCA
effects, the lines derived from LSC could be used as parent in crosses
to increase GY. The maximum best- parent heterosis values and
highest SCA effects resulted from crosses B73 × MO17 and A679 ×
MO17 for GY. The best heterotic patterns were LSC × RYD, which
would be potentially useful in maize breeding programs to obtain
high-yielding hybrids in the same climate of Iran.
Abstract: The common bean is the most important grain legume for direct human consumption in the world and BCMV is one of the world's most serious bean diseases that can reduce yield and quality of harvested product. To determine the best tolerance index to BCMV and recognize tolerant genotypes, 2 experiments were conducted in field conditions. Twenty five common bean genotypes were sown in 2 separate RCB design with 3 replications under contamination and non-contamination conditions. On the basis of the results of indices correlations GMP, MP and HARM were determined as the most suitable tolerance indices. The results of principle components analysis indicated 2 first components totally explained 98.52% of variations among data. The first and second components were named potential yield and stress susceptible respectively. Based on the results of BCMV tolerance indices assessment and biplot analysis WA8563-4, WA8563-2 and Cardinal were the genotypes that exhibited potential seed yield under contamination and noncontamination conditions.
Abstract: The paper makes part from a complex research project
on Romanian Grey Steppe, a unique breed in terms of biological and
cultural-historical importance, on the verge of extinction and which
has been included in a preservation programme of genetic resources
from Romania. The study of genetic polymorphism of protean
fractions, especially kappa-casein, and the genotype relations of
these lactoproteins with some quantitative and qualitative features of
milk yield represents a current theme and a novelty for this breed. In
the estimation of the genetic parameters we used R.E.M.L.
(Restricted Maximum Likelihood) method.
The main lactoprotein from milk, kappa - casein (K-cz),
characterized in the specialized literature as a feature having a high
degree of hereditary transmission, behaves as such in the nucleus under
study, a value also confirmed by the heritability coefficient (h2 = 0.57
%). We must mention the medium values for milk and fat quantity
(h2=0.26, 0.29 %) and the fat and protein percentage from milk
having a high hereditary influence h2 = 0.71 - 0.63 %.
Correlations between kappa-casein and the milk quantity are
negative and strong. Between kappa-casein and other qualitative
features of milk (fat content 0.58-0.67 % and protein content 0.77-
0.87%), there are positive and very strong correlations. At the same
time, between kappa-casein and β casein (β-cz), β lactoglobulin (β-
lg) respectively, correlations are positive having high values (0.37 –
0.45 %), indicating the same causes and determining factors for the
two groups of features.