Abstract: The biomass-based fuels have become great concern in order to replace the petroleum-based fuels. Biofuels are a wide range of fuels referred to liquid, gas and solid fuels produced from biomass. Recently, higher chain alcohols such as 3-methyl-1-butanol and isobutanol have become a better candidate compared to bioethanol in order to replace gasoline as transportation fuel. Therefore, in this study, 3-methyl-1-butanol was produced through a fermentation process by yeast. Several types of yeast involved in this research including Saccharomyces cerevisiae, Kluyveromyces lactis GG799 and Pichia pastoris (KM71H, GS115 and X33). The result obtained showed that K. lactis GG799 gave the highest concentration of 3-methyl-1-butanol at 274 mg/l followed by S. cerevisiae, P. pastoris GS115, P. pastoris KM71H and P. pastoris X33 at 265 mg/l, 190 mg/l, 182 mg/l and 174 mg/l respectively. Based on the result, it proved that yeast have a potential in producing 3-methyl-1-butanol naturally.
Abstract: Compost can influence soil fertility and plant health. At the same time compost can play an important role in the nitrogen cycle and it can influence leaching of mineral nitrogen from soil to underground water.
This paper deals with the influence of compost addition and mineral nitrogen fertilizer on leaching of mineral nitrogen, nitrogen availability in microbial biomass and plant biomass production in the lysimetric experiment. Twenty one lysimeters were filed with topsoil and subsoil collected in the area of protection zone of underground source of drinking water - Březová nad Svitavou. The highest leaching of mineral nitrogen was detected in the variant fertilized only mineral nitrogen fertilizer (624.58 mg m-2), the lowest leaching was recorded in the variant with high addition of compost (315.51 mg m-2). On the other hand, losses of mineral nitrogen are not in connection with the losses of available form of nitrogen in microbial biomass. Because lost of mineral nitrogen was detected in variant with the least change in the availability of N in microbial biomass.
The leaching of mineral nitrogen, yields as well as the results concerning nitrogen availability from the first year of long term experiment suggest that compost can positive influence the leaching of nitrogen into underground water.
Abstract: Present paper deals with an evaluation of magnitude of changes in biomass and net primary productivity at ‘Gujar Tal’ sloppy lake margin at Jaunpur in tropical semi-arid region of eastern U.P. (India). The study site abandoned or neglected lands (50 ×125 m) was divided into two zones, i.e. upper zone (up-land) and lower zone (low-land). Maximum biomass in the upper zone of dominant weed Desmostachya bipinnata (L.) Stapf. was 207.47 g m-2 and ‘rest weeds’ was 457.45 g m-2 both in the month of September. In contrast, the peak biomass value in the lower zone of dominant weed Oryza rufipogon Griff. was 1571.44 g m-2 in October and ‘rest weeds’ 270.65 g m-2 in February. Among the two zones, the peak total community biomass was observed 1655.62 g m-2 (October) in the lower zone while its peak value for the upper zone 457.45 g m-2 (September) was comparatively low. Maximum percentage contribution of dominant weeds (D. bipinnata and O. rufipogon) in the respective upper and lower zones and ‘rest weeds’ in both the zones varied in different months in the total community biomass. The peak net primary productivity of dominant weed (D. bipinnata) was 2.09g m-2 day-1 (September) and ‘rest weeds’ was 2.37 g m-2 day-1 (August) in the upper zone, while the lower zone for O. rufipogon was 5.25 g m-2 day-1 (June) as this zone was inundated later and ‘rest weeds’ was 2.08 g m-2 day-1 (January, 2009). The annual net production of total community at site I was highest, 409.58 g m-2 yr-1 in the upper zone followed by 395.58 g m-2 per eight month in the lower zone as this zone was flooded with water during rainy season. The site significance of variations in biomass in relation to plant species was tested by analysis of variance. It was significant between months in all the two zones (p
Abstract: In order to fulfill world energy demand, several efforts have been done to look for new and renewable energy candidates to substitute oil and gas. Biomass is one of new and renewable energy sources, which is abundant in Indonesia. Palm kernel shell is a kind of biomass discharge from palm oil industries as a waste. On the other hand, Jatropha curcas that is easy to grow in Indonesia is also a typical energy source either for bio-diesel or biomass. In this study, biomass was used as co-fuel in briquetting of low-rank coal to suppress the release of emission (such as CO, NOx and SOx) during coal combustion. Desulfurizer, CaO-base, was also added to ensure the SOx capture is effectively occurred. Ratio of coal to palm kernel shell (w/w) in the bio-briquette were 50:50, 60:40, 70:30, 80:20 and 90:10, while ratio of calcium to sulfur (Ca/S) in mole/mole were 1:1; 1.25:1; 1.5:1; 1.75:1 and 2:1. The bio-briquette then subjected to physical characterization and combustion test. The results show that the maximum weight loss in the durability measurement was ±6%. In addition, the highest stove efficiency for each desulfurizer was observed at the coal/PKS ratio of 90:10 and Ca/S ratio of 1:1 (except for the scallop shell desulfurizer that appeared at two Ca/S ratios; 1.25:1 and 1.5:1, respectively), i.e. 13.8% for the lime; 15.86% for the oyster shell; 14.54% for the scallop shell and 15.84% for the green mussel shell desulfurizers.
Abstract: This study investigated the effect of a dilute acid, lime and ammonia aqueous pretreatment on the fermentable sugars conversion from empty fruit bunch (EFB) biomass. The dilute acid treatment was carried out in an autoclave, at 121ºC with 4% of sulfuric acid. In the lime pretreatment, 3 wt % of calcium hydroxide was used, whereas the third method was done by soaking EFB with 28% ammonia solution. The EFB biomass was then subjected to a two-stage-acid hydrolysis process. Subsequently, the hydrolysate was fermented by using instant baker’s yeast to produce bioethanol. The highest glucose yield was 890 mg/g of biomass, obtained from the sample which underwent lime pretreatment. The highest bioethanol yield of 6.1mg/g of glucose was achieved from acid pretreatment. This showed that the acid pretreatment gave the most fermentable sugars compared to the other two pretreatments.
Abstract: Grasslands of Iran are encountered with a vast
desertification and destruction. Some legumes are plants of forage
importance with high palatability. Studied legumes in this project are
Onobrychis, Medicago sativa (alfalfa) and Trifolium repens. Seeds
were cultivated in research field of Kaboutarabad (33 km East of
Isfahan, Iran) with an average 80 mm. annual rainfall. Plants were
cultivated in a split plot design with 3 replicate and two water
treatments (weekly irrigation, and under stress with same amount per
15 days interval). Water entrance to each plots were measured by
Partial flow. This project lasted 20 weeks. Destructive samplings
(1m2 each time) were done weekly. At each sampling plants were
gathered and weighed separately for each vegetative parts. An Area
Meter (Vista) was used to measure root surface and leaf area. Total
shoot and root fresh and dry weight, leaf area index and soil coverage
were evaluated too. Dry weight was achieved in 750c oven after 24
hours. Statgraphic and Harvard Graphic software were used to
formulate and demonstrate the parameters curves due to time. Our
results show that Trifolium repens has affected 60 % and Medicago
sativa 18% by water stress. Onobrychis total fresh weight was
reduced 45%. Dry weight or Biomass in alfalfa is not so affected by
water shortage. This means that in alfalfa fields we can decrease the
irrigation amount and have some how same amount of Biomass.
Onobrychis show a drastic decrease in Biomass. The increases in
total dry matter due to time in studied plants are formulated. For
Trifolium repens if removal or cattle entrance to meadows do not
occurred at perfect time, it will decrease the palatability and water
content of the shoots. Water stress in a short period could develop the
root system in Trifolium repens, but if it last more than this other
ecological and soil factors will affect the growth of this plant. Low
level of soil water is not so important for studied legume forges. But
water shortage affect palatability and water content of aerial parts.
Leaf area due to time in studied legumes is formulated. In fact leaf
area is decreased by shortage in available water. Higher leaf area
means higher forage and biomass production. Medicago and
Onobrychis reach to the maximum leaf area sooner than Trifolium
and are able to produce an optimum soil cover and inhibit the
transpiration of soil water of meadows. Correlation of root surface to
Total biomass in studied plants is formulated. Medicago under water
stress show a 40% decrease in crown cover while at optimum
condition this amount reach to 100%. In order to produce forage in
areas without soil erosion Medicago is the best choice even with a
shortage in water resources. It is tried to represent the growth
simulation of three famous Forage Legumes. By growth simulation
farmers and range managers could better decide to choose best plant
adapted to water availability without designing different time and
labor consuming field experiments.
Abstract: Enzymatic hydrolysis is one of the major steps involved in the conversion from sugarcane bagasse to yield ethanol. This process offers potential for yields and selectivity higher, lower energy costs and milder operating conditions than chemical processes. However, the presence of some factors such as lignin content, crystallinity degree of the cellulose, and particle sizes, limits the digestibility of the cellulose present in the lignocellulosic biomasses. Pretreatment aims to improve the access of the enzyme to the substrate. In this study sugarcane bagasse was submitted chemical pretreatment that consisted of two consecutive steps, the first with dilute sulfuric acid (1 % (v/v) H2SO4), and the second with alkaline solutions with different concentrations of NaOH (1, 2, 3 and 4 % (w/v)). Thermal Analysis (TG/ DTG and DTA) was used to evaluate hemicellulose, cellulose and lignin contents in the samples. Scanning Electron Microscopy (SEM) was used to evaluate the morphological structures of the in natura and chemically treated samples. Results showed that pretreatments were effective in chemical degradation of lignocellulosic materials of the samples, and also was possible to observe the morphological changes occurring in the biomasses after pretreatments.
Abstract: One of the major pollutants in the environment is arsenic (As). Due to the toxic effects of As to all organisms, its remediation is necessary. Conventional technologies used in the remediation of As contaminated soils are expensive and may even compromise the structure of the soil. An attractive alternative is phytoremediation, which is the use of plants which can take up the contaminant in their tissues. Plant growth promoting bacteria (PGPB) has been known to enhance growth of plants through several mechanisms such as phytohormone production, phosphate solubilization, siderophore production and 1-aminocyclopropane-1- carboxylate (ACC) deaminase production, which is an essential trait that aids plants especially under stress conditions such as As stress. Twenty one bacteria were isolated from As-contaminated soils in the vicinity of the Janghang Smelter in Chungnam Province, South Korea. These exhibited high tolerance to either arsenite (As III) or arsenate (As V) or both. Most of these isolates possess several plant growth promoting traits which can be potentially exploited to increase phytoremediation efficiency. Among the identified isolates is Pseudomonas sp. JS1215, which produces ACC deaminase, indole acetic acid (IAA), and siderophore. It also has the ability to solubilize phosphate. Inoculation of JS1215 significantly enhanced root and shoot length and biomass accumulation of maize under normal conditions. In the presence of As, particularly in lower As level, inoculation of JS1215 slightly increased root length and biomass. Ethylene increased with increasing As concentration, but was reduced by JS1215 inoculation. JS1215 can be a potential bioinoculant for increasing phytoremediation efficiency.
Abstract: Technology transfer of renewable energy technologies is very often unsuccessful in the developing world. Aside from challenges that have social, economic, financial, institutional and environmental dimensions, technology transfer has generally been misunderstood, and largely seen as mere delivery of high tech equipment from developed to developing countries or within the developing world from R&D institutions to society. Technology transfer entails much more, including, but not limited to: entire systems and their component parts, know-how, goods and services, equipment, and organisational and managerial procedures. Means to facilitate the successful transfer of energy technologies, including the sharing of lessons are subsequently extremely important for developing countries as they grapple with increasing energy needs to sustain adequate economic growth and development. Improving the success of technology transfer is an ongoing process as more projects are implemented, new problems are encountered and new lessons are learnt. Renewable energy is also critical to improve the quality of lives of the majority of people in developing countries. In rural areas energy is primarily traditional biomass. The consumption activities typically occur in an inefficient manner, thus working against the notion of sustainable development. This paper explores the implementation of technology transfer in the developing world (sub-Saharan Africa). The focus is necessarily on RETs since most rural energy initiatives are RETs-based. Additionally, it aims to highlight some lessons drawn from the cited RE projects and identifies notable differences where energy technology transfer was judged to be successful. This is done through a literature review based on a selection of documented case studies which are judged against the definition provided for technology transfer. This paper also puts forth research recommendations that might contribute to improved technology transfer in the developing world. Key findings of this paper include: Technology transfer cannot be complete without satisfying pre-conditions such as: affordability, maintenance (and associated plans), knowledge and skills transfer, appropriate know how, ownership and commitment, ability to adapt technology, sound business principles such as financial viability and sustainability, project management, relevance and many others. It is also shown that lessons are learnt in both successful and unsuccessful projects.
Abstract: The oleaginous yeasts Lipomyces starkey were grown
in the presence of dairy industry wastewaters (DIW). The yeasts were
able to degrade the organic components of DIW and to produce a
significant fraction of their biomass as triglycerides.
When using DIW from the Ricotta cheese production or residual
whey as growth medium, the L. starkey could be cultured without
dilution nor external organic supplement. On the contrary, the yeasts
could only partially degrade the DIW from the Mozzarella cheese
production, due to the accumulation of a metabolic product beyond
the threshold of toxicity. In this case, a dilution of the DIW was
required to obtain a more efficient degradation of the carbon
compounds and an higher yield in oleaginous biomass.
The fatty acid distribution of the microbial oils obtained showed a
prevalence of oleic acid, and is compatible with the production of a II
generation biodiesel offering a good resistance to oxidation as well as
an excellent cold-performance.
Abstract: Organic farmers across Saskatchewan face soil
phosphorus (P) shortages. Due to the restriction on inputs in organic
systems, farmers rely on crop rotation and naturally-occurring
arbuscular mycorrhizal fungi (AMF) for plant P supply. Crop rotation
is important for disease, pest, and weed management. Crops that are
not colonized by AMF (non-mycorrhizal) can decrease colonization
of a following crop. An experiment was performed to quantify soil P
cycling in four cropping sequences under organic management and
determine if mustard (non-mycorrhizal) was delaying the
colonization of subsequent wheat. Soils from the four cropping
sequences were measured for inorganic soil P (Pi), AMF spore
density (SD), phospholipid fatty acid analysis (PLFA, for AMF
biomarker counts), and alkaline phosphatase activity (ALPase,
related to AMF metabolic activity). Plants were measured for AMF
colonization and P content and uptake of above-ground biomass. A
lack of difference in AMF activity indicated that mustard was not
depressing colonization. Instead, AMF colonization was largely
determined by crop type and crop rotation.
Abstract: Seasonal variability of nutrients concentration in the Baltic Sea using the 3D ecosystem numerical model 3D-CEMBS has been investigated. Additionally this study shows horizontal and vertical distribution of nutrients in the Baltic Sea. Model domain is an extended Baltic Sea area divided into 600x640 horizontal grid cells. Aside from standard hydrodynamic parameters 3D-CEMBS produces modeled ecological variables such as: three types of phytoplankton, two detrital classes, dissolved oxygen and the nutrients (nitrate, ammonium, phosphate and silicate). The presented model allows prediction of parameters that describe distribution of nutrients concentration and phytoplankton biomass. 3D-CEMBS can be used to study the effect of different hydrodynamic and biogeochemical processes on distributions of these variables in a larger scale.
Abstract: It was determined that woody biomass and livestock excreta can be utilized as hydrogen resources and hydrogen produced from such sources can be used to fill fuel cell vehicles (FCVs) at hydrogen stations. It was shown that the biomass transport costs for hydrogen production may be reduced the costs for co-generation. In the Tokyo Metropolitan Area, there are only a few sites capable of producing hydrogen from woody biomass in amounts greater than 200 m3/h-the scale required for a hydrogen station to be operationally practical. However, in the case of livestock excreta, it was shown that 15% of the municipalities in this area are capable of securing sufficient biomass to be operationally practical for hydrogen production. The differences in feasibility of practical operation depend on the type of biomass.
Abstract: Lignocellulosic materials are new targeted source to
produce second generation biofuels like biobutanol. However, this
process is significantly resisted by the native structure of biomass.
Therefore, pretreatment process is always essential to remove
hemicelluloses and lignin prior to the enzymatic hydrolysis.
The goals of pretreatment are removing hemicelluloses and
lignin, increasing biomass porosity, and increasing the enzyme
accessibility. The main goal of this research is to study the important
variables such as pretreatment temperature and time, which can give
the highest total sugar yield in pretreatment step by using dilute
phosphoric acid. After pretreatment, the highest total sugar yield of
13.61 g/L was obtained under an optimal condition at 140°C for 10
min of pretreatment time by using 1.75% (w/w) H3PO4 and at 15:1
liquid to solid ratio. The total sugar yield of two-stage process
(pretreatment+enzymatic hydrolysis) of 27.38 g/L was obtained.
Abstract: Enzymatic saccharification of biomass for reducing
sugar production is one of the crucial processes in biofuel production
through biochemical conversion. In this study, enzymatic
saccharification of dilute potassium hydroxide (KOH) pre-treated
Tetraselmis suecica biomass was carried out by using cellulase
enzyme obtained from Trichoderma longibrachiatum. Initially, the
pre-treatment conditions were optimised by changing alkali reagent
concentration, retention time for reaction, and temperature. The T.
suecica biomass after pre-treatment was also characterized using
Fourier Transform Infrared Spectra and Scanning Electron
Microscope. These analyses revealed that the functional group such
as acetyl and hydroxyl groups, structure and surface of T. suecica
biomass were changed through pre-treatment, which is favourable for
enzymatic saccharification process. Comparison of enzymatic
saccharification of untreated and pre-treated microalgal biomass
indicated that higher level of reducing sugar can be obtained from
pre-treated T. suecica. Enzymatic saccharification of pre-treated T.
suecica biomass was optimised by changing temperature, pH, and
enzyme concentration to solid ratio ([E]/[S]). Highest conversion of
carbohydrate into reducing sugar of 95% amounted to reducing sugar
yield of 20 (wt%) from pre-treated T. suecica was obtained from
saccharification, at temperature: 40°C, pH: 4.5 and [E]/[S] of 0.1
after 72 h of incubation. Hydrolysate obtained from enzymatic
saccharification of pretreated T. suecica biomass was further
fermented into biobutanol using Clostridium saccharoperbutyliticum
as biocatalyst. The results from this study demonstrate a positive
prospect of application of dilute alkaline pre-treatment to enhance
enzymatic saccharification and biobutanol production from
microalgal biomass.
Abstract: Pretreatment of lignocellulosic biomass materials from
poplar, acacia, oak, and fir with different ionic liquids (ILs)
containing 1-alkyl-3-methyl-imidazolium cations and various anions
has been carried out. The dissolved cellulose from biomass was
precipitated by adding anti-solvents into the solution and vigorous
stirring. Commercial cellulases Celluclast 1.5L and Accelerase 1000
have been used for hydrolysis of untreated and pretreated
lignocellulosic biomass. Among the tested ILs, [Emim]COOCH3
showed the best efficiency, resulting in highest amount of liberated
reducing sugars. Pretreatment of lignocellulosic biomass using
glycerol-ionic liquids combined pretreatment and dilute acid-ionic
liquids combined pretreatment were evaluated and compared with
glycerol pretreatment, ionic liquids pretreatment and dilute acid
pretreatment.
Abstract: Pentachlorophenol (PCP) is a polychlorinated
aromatic compound that is widespread in industrial effluents and is
considered to be a serious pollutant. Among the variety of industrial
effluents encountered, effluents from tanning industry are very
important and have a serious pollution potential. PCP is also formed
unintentionally in effluents of paper and pulp industries. It is highly
persistent in soils and is lethal to a wide variety of beneficial
microorganisms and insects, human beings and animals. The natural
processes that breakdown toxic chemicals in the environment have
become the focus of much attention to develop safe and environmentfriendly
deactivation technologies. Microbes and plants are among
the most important biological agents that remove and degrade waste
materials to enable their recycling in the environment. The present
investigation was carried out with the aim of developing a microbial
system for bioremediation of PCP polluted soils. A number of plant
species were evaluated for their ability to tolerate different
concentrations of pentachlorophenol (PCP) in the soil. The
experiment was conducted for 30 days under pot culture conditions.
The toxic effect of PCP on plants was studied by monitoring seed
germination, plant growth and biomass. As the concentration of PCP
was increased to 50 ppm, the inhibition of seed germination, plant
growth and biomass was also increased. Although PCP had a
negative effect on all plant species tested, maize and groundnut
showed the maximum tolerance to PCP. Other tolerating crops
included wheat, safflower, sunflower, and soybean. From the
rhizosphere soil of the tolerant seedlings, as many as twenty seven
PCP tolerant bacteria were isolated. From soybean, 8; sunflower, 3;
safflower 8; maize 2; groundnut and wheat, 3 each isolates were
made. They were screened for their PCP degradation potentials.
HPLC analyses of PCP degradation revealed that the isolate MAZ-2
degraded PCP completely. The isolate MAZ-1 was the next best
isolate with 90 per cent PCP degradation. These strains hold promise
to be used in the bioremediation of PCP polluted soils.
Abstract: In order to evaluate the relationship between the sulphur (S), glucose (G), nitrogen (N) and plant residues (st), sulphur immobilization and microbial transformation were monitored in five soil samples from 0-30 cm of Bastam farmers fields of Shahrood area following 11 treatments with different levels of Sulphur (S), glucose (G), N and plant residues (wheat straw) in a randomized block design with three replications and incubated over 20, 45 and 60 days, the immobilization of SO4 -2-S presented as a percentage of that added, was inversely related to its addition rate. Additions of glucose and plant residues increased with the C-to-S ratio of the added amendments, irrespective of their origins (glucose and plant residues). In the presence of C sources (glucose or plant residues). N significantly increased the immobilization of SO4 -2-S, whilst the effect of N was insignificant in the absence of a C amendment. In first few days the amounts of added SO4 -2-S immobilized were linearly correlated with the amounts of added S recovered in the soil microbial biomass. With further incubation the proportions of immobilized SO4 -2-S remaining as biomass-S decreased. Decrease in biomass-S was thought to be due to the conversion of biomass-S into soil organic-S. Glucose addition increased the immobilization (microbial utilization and incorporation into the soil organic matter) of native soil SO4 -2-S. However, N addition enhance the mineralization of soil organic-S, increasing the concentration of SO4 - 2-S in soil.
Abstract: Biomass is becoming a large renewable resource for
power generation; it is involved in higher frequency in
environmentally clean processes, and even it is used for biofuels
preparation. On the other hand, hydrogen – other energy source – can
be produced in a variety of methods including gasification of
biomass. In this study, the production of hydrogen by gasification of
biomass waste is examined. This work explores the production of a
gaseous mixture with high power potential from Amazonas´ specie
known as copoazu, using a counter-flow fixed-bed bioreactor.
Abstract: Biofuels, like biobutanol, have been recognized for
being renewable and sustainable fuels which can be produced from
lignocellulosic biomass. To convert lignocellulosic biomass to
biofuel, pretreatment process is an important step to remove
hemicelluloses and lignin to improve enzymatic hydrolysis. Dilute
acid pretreatment has been successful developed for pretreatment of
corncobs and the optimum conditions of dilute sulfuric and
phosphoric acid pretreatment were obtained at 120 °C for 5 min with
15:1 liquid to solid ratio and 140 °C for 10 min with 10:1 liquid to
solid ratio, respectively. The result shows that both of acid
pretreatments gave the content of total sugar approximately 34–35
g/l. In case of inhibitor content (furfural), phosphoric acid
pretreatment gives higher than sulfuric acid pretreatment.
Characterizations of corncobs after pretreatment indicate that both of
acid pretreatments can improve enzymatic accessibility and the better
results present in corncobs pretreated with sulfuric acid in term of
surface area, crystallinity, and composition analysis.