Effect of Temperature and Time on Dilute Acid Pretreatment of Corn Cobs

Lignocellulosic materials are new targeted source to produce second generation biofuels like biobutanol. However, this process is significantly resisted by the native structure of biomass. Therefore, pretreatment process is always essential to remove hemicelluloses and lignin prior to the enzymatic hydrolysis. The goals of pretreatment are removing hemicelluloses and lignin, increasing biomass porosity, and increasing the enzyme accessibility. The main goal of this research is to study the important variables such as pretreatment temperature and time, which can give the highest total sugar yield in pretreatment step by using dilute phosphoric acid. After pretreatment, the highest total sugar yield of 13.61 g/L was obtained under an optimal condition at 140°C for 10 min of pretreatment time by using 1.75% (w/w) H3PO4 and at 15:1 liquid to solid ratio. The total sugar yield of two-stage process (pretreatment+enzymatic hydrolysis) of 27.38 g/L was obtained.




References:
[1] Icoz, E., Turgrul, K.M.M., Saral, A.M., and Icoz, E. (2009).Research on
ethanol production and use from sugar beet Turkey. Biomass and
Bioenergy, 33, 1-7.
[2] Palmqvist, E. and Hahn-Hagerdal, B. (2002). Fermentation of
lignocellulosic hydrolysates. : inhibitors and mechanisms of inhibition.
Bioresource Technology, 74(1), 25-33.
[3] Zheng, Y., Pan, Z.L., and Zhang, R.H. (2009). Overview of biomass
pretreatment for cellulosic ethanol production. Agricultural and
Biological Engineering, 2,51-68.
[4] Hendriks, A.T.W.M., and Zeeman, G. (2009). Pretreatment to enhance
the digestion digestibility of lignocellulosic biomass.Bioresource
Technology, 100, 10-18.
[5] Gamez, S.R., Gonzalez-Cabriales, J.J., Ramirez, J.A.B.T.,Garrote, G.,
Vazquez, M.N. (2006). Study of the hydrolysis of sugar cane bagasses
using phosphoric acid. Journal of Food Engineering, 74, 78-88.
[6] Gamez, S., Ramirez, J.A., Garrote, G., Vazquez, M. (2004).
Manufacture of Fermentable Sugar Solutions from Sugar Cane Bagasse
Hydrolyzed with Phosphoric Acid at atmospheric Pressure, 74, 4172-
4177.
[7] Kumar, P.V., Barrett, D.M., Delwiche, M.C., Stroeve, P.T. (2009).
Methods for pretreatment of lignocellulosic biomass for efficient
hydrolysis and biofuels production. Industrial and Engineering
Chemistry Research, 48, 3713-3729.
[8] Ezeji, T., Qureshi, N., and Blaschek, H.P. (2007). Butanol Production
From Agricultural Residues: Impact of Degradation Products on
Clostridium beijerinckii Growth and Butanol Fermentation.
Biotechnology ans Bioengineering, 97, 1460-1469.
[9] Vazquez, M., Oliva, M., Tellez-Luis, S.J. and Ramirez, J.A. (2007).
Hydrolysis of sorghum straw using phosphoric acid:Evaluation of
furfural production. Bioresource Technology, 98, 3053-3060.
[10] Weil, J R., Dien, B., Bothast, R., Hendrickson, R., Mosier, N.T. S., and
Ladisch M.M. R. (2002). Removal of Fermentation Inhibitors Formed
during Pretreatment of Biomass by Polymeric Adsorbents. Industry and
Engineering Chemistry Research, 41, 6132-6138.
[11] Redding, A.T., Wang, Z.Y., Keshwani, D.P., Cheng, J. (2011). High
temperature dilute acid pretreatment of coastal Bermuda grass for
enzymatic hydrolysis. Bioresource Technology, 102, 1415-1424.
[12] Almeida, J., Bertilsson, M., Gorwa-Grauslund, M., Gorsich, S., Gunnar,
L., (2009). Metabolic effects of fraldehydes and impacts on
biotechnological processes. Applied Micribiology and. Biotechnology.
82, 625-638.
[13] Delgenes, J., Moletta, R., Navarro, J., (1996). Effect of lignocellulosic
degradation products on ethanol fermentation of glucose and xylose by
Saccharomycescerevisiae, Zymomonas mobilis, Pichia stipitis, and
Candida shehatae. Enzyme and Microbial Technology, 19, 220-225.
[14] Taherzadeh, M., Eklund, R., Gustafsson, L., Niklasson, C., Liden, G.,
(1997). Characterization and fermentation of dilute acid hydrolyzates
from wood. Industrial and Engineering Chemistry Research, 36, 4659-
4665.