Abstract: The adverse effects of Clindamycin (Clind.) /
Ibuprofen (Ibu.) combination on liver, kidney, blood elements and the
significances of antioxidants (N-acetylcysteine and Zinc) against
these effects were evaluated. The study includes: Group I; control
n=30, Group II; patients on Clind.300mg/Ibu.400mg twice daily for a
week n=30, Group III; patients on Clind.300mg/Ibu.400mg+Nacetylcysteine
200mg twice daily for a week n=15 and Group IV;
patients on Clind.300mg/Ibu.400mg+Zinc50mg twice daily for a
week n=15. Serum malondialdehyde (MDA), alanine transferase
(ALT), aspartate transferase (AST), γ glutamyl transferase (GGT),
creatinine, blood urea nitrogen (BUN) were measured. Applying one
way ANOVA followed by Tuckey Kramer post test, Group II showed
significant increase in ALT, AST, GGT, BUN and decrease in Hb,
RBCs, platelets than Group I. Group III showed significant decrease
in ALT, AST, GGT, BUN than Group II. Moreover, Group IV
showed significant decrease in ALT, AST, GGT and increase in Hb,
RBCs, and platelets than Group II. Conclusively, Adding Zinc or Nacetylcysteine
buffer the oxidative stress and improve the therapeutic
outcome of Clindamycin/Ibuprofen combination.
Abstract: Our results showed that for the growth of qualitative
seedling and vegetative raw material of ðó. marschallianus Willd. and
T. serphyllum L. it is more profitable to use the in vitro and
hydroponics combined method. In in vitro culture it is possible to do
micro-propagation whole year with 98-99% rhizogenesis. 30000
micro-plants were obtained from one explant during 9 months.
Hydroponic conditions provide the necessary microclimate for
microplants where the survival rate without acclimatization was
93.3%. The essential oil content in hydroponic dry herb of both
species in vegetative and blossom phase was 1.3% whereas in wild
plants it was 1.2%, the content of extractive substances and vitamin
C also exceeded wild plants. Our biochemical and radiochemical
investigations indicated that the medicinal raw materials obtained
from hydroponic and wild plants of Thymus species correspond to
the demands of SPh XI, and the content of artificial radionuclides
does not exceed the MACL.
Abstract: Biochemical and molecular analysis of some
antioxidant enzyme genes revealed different level of gene expression
on oilseed (Brassica napus). For molecular and biochemical
analysis, leaf tissues were harvested from plants at eight different
developmental stages, from young to senescence. The levels of total
protein and chlorophyll were increased during maturity stages of
plant, while these were decreased during the last stages of plant
growth. Structural analysis (nucleotide and deduced amino acid
sequence, and phylogenic tree) of a complementary DNA revealed a
high level of similarity for a family of Catalase genes. The
expression of the gene encoded by different Catalase isoforms was
assessed during different plant growth phase. No significant
difference between samples was observed, when Catalase activity
was statistically analyzed at different developmental stages. EST
analysis exhibited different transcripts levels for a number of other
relevant antioxidant genes (different isoforms of SOD and
glutathione). The high level of transcription of these genes at
senescence stages was indicated that these genes are senescenceinduced
genes.
Abstract: The effects of seawater and slurry ice bleeding methods on the sensory, microbiological and chemical quality changes of cod fillets during chilled storage were examined in this study. The results from sensory evaluation showed that slurry ice bleeding method prolonged the shelf life of cod fillets up to 13-14 days compared to 10-11 days for fish bled in seawater. Slurry ice bleeding method also led to a slower microbial growth and biochemical developments, resulting lower total plate count (TPC), H2S-producing bacteria count, total volatile basic nitrogen (TVB-N), trimethylamine (TMA), free fatty acid (FFA) content and higher phospholipid content (PL) compared to those of samples bled in seawater. The results of principle component analysis revealed that TPC, H2S-producing bacteria, TVB-N, TMA and FFA were in significant correlation. They were also in negative correlation with sensory evaluation (Torry score), PL and water holding capacity (WHC).
Abstract: The understanding of the system level of biological behavior and phenomenon variously needs some elements such as gene sequence, protein structure, gene functions and metabolic pathways. Challenging problems are representing, learning and reasoning about these biochemical reactions, gene and protein structure, genotype and relation between the phenotype, and expression system on those interactions. The goal of our work is to understand the behaviors of the interactions networks and to model their evolution in time and in space. We propose in this study an ontological meta-model for the knowledge representation of the genetic regulatory networks. Ontology in artificial intelligence means the fundamental categories and relations that provide a framework for knowledge models. Domain ontology's are now commonly used to enable heterogeneous information resources, such as knowledge-based systems, to communicate with each other. The interest of our model is to represent the spatial, temporal and spatio-temporal knowledge. We validated our propositions in the genetic regulatory network of the Aarbidosis thaliana flower
Abstract: Visfatin and apelin are two new adipokines that recently gained a special interest in diabetes research. This study was conducted to study the interplay between these two adipokines and their correlation with other inflammatory and biochemical parameters in type 2 diabetic (T2D) postmenopausal women with CAD. Visfatin and apelin were measured by enzyme-linked immunoassay (ELISA). Visfatin was found to be significantly higher in the following groups: T2D patients without CAD, non-obese and obese T2D patients with CAD when compared to control group. Apelin was found to be significantly lower in non-obese and obese T2D patients with CAD when compared to control group. Visfatin and apelin were found to be significantly associated with each other and with other biochemical parameters. The current study provides evidence for the interplay between visfatin and apelin through the inflammatory milieu characteristic of T2D and their possible role in the pathogenesis of CAD complication of T2D.
Abstract: The possible therapeutic effect of cannabidiol, the major non-psychotropic Cannabis constituent, was investigated against acute hepatotoxicity induced by a single oral dose of acetaminophen (500mg/kg) in mice. Cannabidiol (two intraperitoneal injections, 5mg/kg, each) was given 1 hour and 12 hours following acetaminophen administration. Acetaminophen administration caused significant elevations of serum alanine aminotransferase, and hepatic malondialdehyde, and nitric oxide levels, and a significant decrease in hepatic reduced glutathione. Cannabidiol significantly attenuated the deterioration in the measured biochemical parameters resulted from acetaminophen administration. Also, histopathological examination showed that cannabidiol markedly attenuated ameliorated acetaminophen-induced liver tissue damage. These results emphasize that cannabidiol represents a potential therapeutic option to protect against acetaminophen hepartotoxicity which is a common clinical problem.
Abstract: Water samples were collected from river Pandu at six
stations where human and animal activities were high. Composite
samples were analyzed for dissolved oxygen (DO), biochemical
oxygen demand (BOD), chemical oxygen demand (COD) , pH values
during dry and wet seasons as well as the harmattan period. The total
data points were used to establish relationships between the
parameters and data were also subjected to statistical analysis and
expressed as mean ± standard error of mean (SEM) at a level of
significance of p
Abstract: Tomato nutrition value, color, flavor of their fruits and products depends mainly on lycopene, β-carotene, ascorbic acid, sugars and their ratio. The two most important carotenoids in fruits of tomato are lycopene, which determined fruits red color, and β- carotene, which accounts for approximately 7% of the tomato carotenoids. Therefore, tomato products and their quality can be well characterized by the content of these elements.Maturity at harvest is very important to composition and quality of tomatoes. This is especially a problem with tomatoes picked green since it is difficult to differentiate between mature and immature-green fruits. Typical and advanced mature-green tomatoes will usually attain a much better flavor than those picked at the immature or partially mature stages.To better understand the synthesis of biochemical compounds, their concentration should be compared not only at the last stage of maturity, but also during all fruit ripening period in different varieties.
Abstract: Five original strains of entomopathogenic bacteria
with insecticidal activity against mosquito larvae of the genera Aedes,
Culex and Anopheles have been isolated from natural conditions in
Armenia and characterized. According to morphological,
physiological and biochemical parameters, all isolates were identified
as Bacillus thuringiensis spp. israelensis (Bti). High larvicidal
activity has been showed by three strains Bti. These strains can be
recommended for industrial production of bacterial preparations.
Abstract: In the present research, two nutraceuticals made from
red grape and walnut that showed previously to improve kidney
dysfunction were incorporated separately into functional foods' bread
made from barley and rice bran. The functional foods were evaluated
in rats in which chronic renal failure was induced through feeding
diet rich in adenine and phosphate (APD). The evaluation based on
assessing kidney function, oxidative stress, inflammatory biomarkers
and body weight gain. Results showed induction of chronic kidney
failure reflected in significant increase in plasma urea, creatinine,
malondialdehyde, tumor necrosis factor- α and low density
lipoprotein cholesterol along with significant reduction of plasma
albumin, and total antioxidant and creatinine clearance and body
weight gain on feeding APD compared to control healthy group.
Feeding the functional foods produced amelioration in the different
biochemical parameters and body weight gain indicating
improvement in kidney function.
Abstract: The Yasuj city stream named the Beshar supply
water for different usages such as aquaculture farms , drinking,
agricultural and industrial usages. Fish processing plants
,Agricultural farms, waste water of industrial zones and hospitals
waste water which they are generate by human activity produce a
considerable volume of effluent and when they are released in to the
stream they can effect on the water quality and down stream aquatic
systems. This study was conducted to evaluate the effects of outflow
effluent from different human activity and point and non point
pollution sources on the water quality and health of the Beshar
river next to Yasuj. Yasuj is the biggest and most important city in
the Kohkiloye and Boyerahmad province . The Beshar River is one
of the most important aquatic ecosystems in the upstream of the
Karun watershed in south of Iran which is affected by point and non
point pollutant sources . This study was done in order to evaluate the
effects of human activities on the water quality and health of the
Beshar river. This river is approximately 190 km in length and
situated at the geographical positions of 51° 20' to 51° 48' E and 30°
18' to 30° 52' N it is one of the most important aquatic ecosystems of
Kohkiloye and Boyerahmad province in south-west Iran. In this
research project, five study stations were selected to examine water
pollution in the Beshar River systems. Human activity is now one of
the most important factors affecting on hydrology and water quality
of the Beshar river. Humans use large amounts of resources to sustain
various standards of living, although measures of sustainability are
highly variable depending on how sustainability is defined. The
Beshar river ecosystems are particularly sensitive and vulnerable to
human activities. The water samples were analyzed, then some
important water quality parameters such as pH, dissolve oxygen
(DO), Biochemical Oxygen Demand (BOD5), Chemical Oxygen
Demand (COD), Total Suspended Solids (TDS),Turbidity,
Temperature, Nitrates (NO3) and Phosphates (PO4) were estimated
at the two stations. The results show a downward trend in the water
quality at the down stream of the city. The amounts of
BOD5,COD,TSS,T,Turbidity, NO3 and PO4 in the down stream
stations were considerably more than the station 1. By contrast the
amounts of DO in the down stream stations were less than to the
station 1. However when effluent discharge consequence of human
activities are released into the Beshar river near the city, the quality
of river are decreases and the environmental problems of the river
during the next years are predicted to rise.
Abstract: Quantitative trait loci (QTL) experiments have yielded
important biological and biochemical information necessary for
understanding the relationship between genetic markers and
quantitative traits. For many years, most QTL algorithms only
allowed one observation per genotype. Recently, there has been an
increasing demand for QTL algorithms that can accommodate more
than one observation per genotypic distribution. The Bayesian
hierarchical model is very flexible and can easily incorporate this
information into the model. Herein a methodology is presented that
uses a Bayesian hierarchical model to capture the complexity of the
data. Furthermore, the Markov chain Monte Carlo model composition
(MC3) algorithm is used to search and identify important markers. An
extensive simulation study illustrates that the method captures the
true QTL, even under nonnormal noise and up to 6 QTL.
Abstract: Solid waste can be considered as an urban burden or
as a valuable resource depending on how it is managed. To meet the
rising demand for energy and to address environmental concerns, a
conversion from conventional energy systems to renewable resources
is essential. For the sustainability of human civilization, an
environmentally sound and techno-economically feasible waste
treatment method is very important to treat recyclable waste. Several
technologies are available for realizing the potential of solid waste as
an energy source, ranging from very simple systems for disposing of
dry waste to more complex technologies capable of dealing with
large amounts of industrial waste. There are three main pathways for
conversion of waste material to energy: thermo chemical,
biochemical and physicochemical. This paper investigates the thermo
chemical conversion of solid waste for energy recovery. The
processes, advantages and dis-advantages of various thermo chemical
conversion processes are discussed and compared. Special attention
is given to Gasification process as it provides better solutions
regarding public acceptance, feedstock flexibility, near-zero
emissions, efficiency and security. Finally this paper presents
comparative statements of thermo chemical processes and introduces
an integrated waste management system.
Abstract: The present study was conducted to investigate the
response of plants exposed to lignite-based thermal power plant
emission. For this purpose, five plant species were collected from 1.0
km distance (polluted site) and control plants were collected from
20.0 km distance (control site) to thermal power plant. The common
tree species Cassia siamea Lamk., Polyalthia longifolia. Sonn,
Acacia longifolia (Andrews) Wild., Azadirachta indica A.Juss, Ficus
religiosa L. were selected as test plants. Photosynthetic pigments
changes (chlorophyll a, chlorophyll b and carotenoids) and rubisco
enzyme modifications were studied. Reduction was observed in the
photosynthetic pigments of plants growing in polluted site and also
large sub unit of the rubisco enzyme was degraded in Azadirachta
indica A. Juss collected from polluted site.
Abstract: The mathematical modeling of different biological
processes is usually used to predict or assess behavior of systems in
which these processes take place. This study deals with mathematical
and computer modeling of bi-substrate enzymatic reactions with
ping-pong mechanism, which play an important role in different
biochemical pathways. Besides that, three models of competitive
inhibition were designed using different software packages. The main
objective of this study is to represent the results from in silico
investigation of bi-substrate enzymatic reactions with ordered pingpong
mechanism in the presence of competitive inhibitors, as well as
to describe in details the inhibition effects. The simulation of the
models with certain kinetic parameters allowed investigating the
behavior of reactions as well as determined some interesting aspects
concerning influence of different cases of competitive inhibition.
Simultaneous presence of two inhibitors, competitive to the S1 and S2
substrates have been studied. Moreover, we have found the pattern of
simultaneous influence of both inhibitors.
Abstract: Atrazine, a herbicide widely used in sugarcane and corn production, is a frequently detected groundwater contaminant. An atrazine-degrading bacterium, strain KB02, was obtained from long-term atrazine-treated sugarcane field soils in Kanchanaburi province of Thailand. Strain KB02 had a rod-to-coccus morphological cycle during growth. Sequence analysis of the PCR product indicated that the 16S rRNA gene in strain KB02 was ranging from 97-98% identical to the same region in Klebsiella sp. Based on biochemical, physiological analysis and 16S rDNA sequence analysis of one representative isolate, strain KB02, the isolates belong to the genus Klebsiella in the family Enterobacteriaceae. Interestingly that the various primers for atzA, B and C failed to amplify genomic DNA of strain KB02. Whereas the expected PCR product of atzA, B and C were obtained from the reference strain, Arthrobacter sp. strain KU001.
Abstract: We demonstrate a nonfaradaic electrochemical impedance spectroscopy measurement of biochemically modified gold plated electrodes using a two-electrode system. The absence of any redox indicator in the impedance measurements provide more precise and accurate characterization of the measured bioanalyte at molecular resolution. An equivalent electrical circuit of the electrodeelectrolyte interface was deduced from the observed impedance data of saline solution at low and high concentrations. The detection of biomolecular interactions was fundamentally correlated to electrical double-layer variation at modified interface. The investigations were done using 20mer deoxyribonucleic acid (DNA) strands without any label. Surface modification was performed by creating mixed monolayer of the thiol-modified single-stranded DNA and a spacer thiol (mercaptohexanol) by a two-step self-assembly method. The results clearly distinguish between the noncomplementary and complementary hybridization of DNA, at low frequency region below several hundreds Hertz.
Abstract: Water quality is a subject of ongoing concern.
Deterioration of water quality has initiated serious management
efforts in many countries. This study endeavors to automatically
classify water quality. The water quality classes are evaluated using 6
factor indices. These factors are pH value (pH), Dissolved Oxygen
(DO), Biochemical Oxygen Demand (BOD), Nitrate Nitrogen
(NO3N), Ammonia Nitrogen (NH3N) and Total Coliform (TColiform).
The methodology involves applying data mining
techniques using multilayer perceptron (MLP) neural network
models. The data consisted of 11 sites of canals in Dusit district in
Bangkok, Thailand. The data is obtained from the Department of
Drainage and Sewerage Bangkok Metropolitan Administration
during 2007-2011. The results of multilayer perceptron neural
network exhibit a high accuracy multilayer perception rate at 96.52%
in classifying the water quality of Dusit district canal in Bangkok
Subsequently, this encouraging result could be applied with plan and
management source of water quality.
Abstract: A game animals – elk (Alces alces), deer (Cervus
elaphus), roe deer (Capreolus capreolus) or wild boar (Sus scrofa
scrofa) - every autumn and winter period provide an excellent
investment, diversification of many consumer meals. In last years
consumption and assortiment of game meat products significantly
increase. Investigations about biochemical composition of game meat
are not very much. The meat of wild animals is more favourable for
human health because it has lower saturated fatty acids content, but
higher content of protein. Therefore the aim of investigations was to
compare biochemical composition of ungulates obtained in
Latvia.Investigations were carried out in wild animals different
regions of Latvia. In the studied samples protein, intramuscular fat,
fatty acids and cholesterol were determined. The biochemical
analysis of 54 samples were done. Results of analysis showed that
protein content 22.36 – 22.92% of all types of meat samples is not
different statistically, significantly lower fat content 1.33 ± 0.88%
had elk meat samples and 1.59 ± 0.59% roe deer samples. Content of
cholesterol was various 64.41 – 95.07% in the ruminant meat
samples of different species. From the dietetic point of view the best
composition of fatty acids has meat samples of roe deer.