Abstract: Physical, sensory properties and hypoglycemic effect of crackers produced from sprouted pigeon pea, unripe plantain and brewers’ spent grain fed to diabetic rats were investigated. Different composite flours were used to produce crackers. Physical and sensory properties of the crackers, the blood serum of the rats and changes in the rat body weight were measured. Spread ratio and break strength of the crackers from different flour blends ranges from 7.01 g to 8.51 g and 1.87 g to 3.01 g respectively. The acceptability of the crackers revealed that Sample A (100% wheat crackers) was not significantly (p>0.05) different from Samples C and D. Feeding the rats with formulated crackers caused an increase in the body weight of the rats but a reduced body weight was observed in diabetic rats fed with normal rat feed. The result indicated that cracker produced from the formulated flour blends caused a significant hypoglycemic effect in diabetic rats and led to a reduction of measured biochemical indices. Therefore, this work showed that consumption of crackers from the above formulated flour blend was able to decrease hyperglycemia in diabetic rats.
Abstract: A number of toxic chlorophenolic compounds are formed during pulp bleaching. The nature and concentration of these chlorophenolic compounds largely depends upon the amount and nature of bleaching chemicals used. These compounds are highly recalcitrant and difficult to remove but are partially removed by the biochemical treatment processes adopted by the paper industry. Identification and estimation of these chlorophenolic compounds has been carried out in the primary and secondary clarified effluents from the paper mill by GCMS. Twenty-six chorophenolic compounds have been identified and estimated in paper mill waste waters. Electrochemical treatment is an efficient method for oxidation of pollutants and has successfully been used to treat textile and oil waste water. Electrochemical treatment using less expensive anode material, stainless steel electrodes has been tried to study their removal. The electrochemical assembly comprised a DC power supply, a magnetic stirrer and stainless steel (316 L) electrode. The optimization of operating conditions has been carried out and treatment has been performed under optimized treatment conditions. Results indicate that 68.7% and 83.8% of cholorphenolic compounds are removed during 2 h of electrochemical treatment from primary and secondary clarified effluent respectively. Further, there is a reduction of 65.1, 60 and 92.6% of COD, AOX and color, respectively for primary clarified and 83.8%, 75.9% and 96.8% of COD, AOX and color, respectively for secondary clarified effluent. EC treatment has also been found to increase significantly the biodegradability index of wastewater because of conversion of non- biodegradable fraction into biodegradable fraction. Thus, electrochemical treatment is an efficient method for the degradation of cholorophenolic compounds, removal of color, AOX and other recalcitrant organic matter present in paper mill waste water.
Abstract: Cisplatin (CIS) is one of the most effective an anticancer drug and also toxic to cells by activating oxidative stress. Oleuropein (OLE) has key role against oxidative stress in mammalian cells, but the role of this antioxidant in the toxicity of CIS remains unknown. The aim of the present study was to investigate the efficacy of OLE on CIS-induced liver damages in male rats. With this aim, male Sprague Dawley rats were randomly assigned to one of eight groups: Control group; the group treated with 7 mg/kg/day CIS; the groups treated with 50, 100 and 200 mg/kg/day OLE (i.p.); and the groups treated with OLE for three days starting at 24 h following CIS injection. After 4 days of injections, serum was provided to assess the blood AST, ALT and LDH values. The liver tissues were removed for histological, biochemical (TAC, TOS and MDA) and genotoxic evaluations. In the CIS treated group, the whole liver tissue showed significant histological changes. Also, CIS significantly increased both the incidence of oxidative stress and the induction of 8-hydroxy-deoxyguanosine (8-OH-dG). Moreover, the rats taking CIS have abnormal results on liver function tests. However, these parameters reached to the normal range after administration of OLE for 3 days. Finally, OLE demonstrated an acceptable high potential and was effective in attenuating CIS-induced liver injury. In this trial, the 200 mg/kg dose of OLE firstly appeared to induce the most optimal protective response.
Abstract: Intense oxidative stress, increased glycated hemoglobin and mineral imbalance represent risk factors for complications in diabetic patients. Cardiovascular complications are most common in these patients, including nephropathy. This study was conducted in 2015 at the Procardia Laboratory in Tîrgu Mureș, Romania on 40 type 2 diabetic adults. Routine biochemical tests were performed on the Konleab 20XTi analyzer (serum glucose, total cholesterol, LDL and HDL cholesterol, triglyceride, creatinine, urea). We also measured serum uric acid, magnesium and calcium concentration by photometric procedures, potassium, sodium and chloride by ion selective electrode, and chromium by atomic absorption spectrometry in a group of patients. Glycated hemoglobin (HbA1c) dosage was made by reflectometry. Urine analysis was performed using the HandUReader equipment. The level of oxidative stress was measured by serum malondialdehyde dosage using the thiobarbituric acid reactive substances method. MDRD (Modification of Diet in Renal Disease) formula was applied for calculation of creatinine-derived glomerular filtration rate. GraphPad InStat software was used for statistical analysis of the data. The diabetic subject included in the study presented high MDA concentrations, showing intense oxidative stress. Calcium was deficient in 5% of the patients, chromium deficiency was present in 28%. The atherogenic cholesterol fraction was elevated in 13% of the patients. Positive correlation was found between creatinine and MDRD-creatinine values (p
Abstract: Biochemical and hormonal changes that occur in both follicular fluid and blood are involved in the control of ovarian physiology. The present study was conducted on follicular fluid and serum samples obtained from 708 buffaloes. Samples were examined for estradiol, progesterone, and cholesterol concentrations in relation to seasonal changes, ovarian follicular size, and stage of estrous cycle. The obtained results revealed that follicular fluid and serum levels of estradiol, progesterone, and cholesterol were significantly lower during summer and autumn when compared to winter and spring seasons. With the increase in follicular size, the follicular fluid levels of progesterone and cholesterol were significantly decreased, while estradiol levels were significantly increased. Estradiol and progesterone levels were significantly higher in follicular fluid than blood, while cholesterol was significantly lower in follicular fluid than serum. In conclusion, the current study threw a light on the hormonal changes in the follicular fluid and blood under the effect of heat stress which could be related to the low fertility of buffalo in the summer.
Abstract: Not all proteins have the same nutritional value, since
protein quality strongly depends on its amino acid composition and
digestibility. The meat of game animals could be a high protein
source because of its well-balanced essential amino acids
composition. Investigations about biochemical composition of game
meat such as wild boar (Sus scrofa scrofa), roe deer (Capreolus
capreolus) and beaver (Castor fiber) are not very much. Therefore,
the aim of the investigation was evaluate protein composition of
game meat hunted in Latvia. The biochemical analysis, evaluation of
connective tissue and essential amino acids in meat samples were
done, the amino acids score were calculate. Results of analysis
showed that protein content 20.88-22.05% of all types of meat
samples is not different statistically. The content of connective tissue
from 1.3% in roe deer till 1.5% in beaver meat allowed classified
game animal as high quality meat. The sum of essential amino acids
in game meat samples were determined 7.05–8.26g100g-1. Roe deer
meat has highest protein content and lowest content of connective
tissues among game meat hunted in Latvia. Concluded that amino
acid score for limiting amino acids phenylalanine and tyrosine is high
and shows high biological value of game meat.
Abstract: Landfill waste is a common problem as it has an
economic and environmental impact even if it is closed. Landfill
waste contains a high density of various persistent compounds such
as heavy metals, organic and inorganic materials. As persistent
compounds are slowly-degradable or even non-degradable in the
environment, they often produce sublethal or even lethal effects on
aquatic organisms. The aims of the present study were to estimate
sublethal effects of the Kairiai landfill (WGS: 55°55‘46.74“,
23°23‘28.4“) leachate on the locomotor activity of rainbow trout
Oncorhynchus mykiss juveniles using the original system package
developed in our laboratory for automated monitoring, recording and
analysis of aquatic organisms’ activity, and to determine patterns of
fish behavioral response to sublethal effects of leachate. Four
different concentrations of leachate were chosen: 0.125; 0.25; 0.5 and
1.0 mL/L (0.0025; 0.005; 0.01 and 0.002 as part of 96-hour LC50,
respectively). Locomotor activity was measured after 5, 10 and 30
minutes of exposure during 1-minute test-periods of each fish (7 fish
per treatment). The threshold-effect-concentration amounted to 0.18
mL/L (0.0036 parts of 96-hour LC50). This concentration was found
to be even 2.8-fold lower than the concentration generally assumed to
be “safe” for fish. At higher concentrations, the landfill leachate
solution elicited behavioral response of test fish to sublethal levels of
pollutants. The ability of the rainbow trout to detect and avoid
contaminants occurred after 5 minutes of exposure. The intensity of
locomotor activity reached a peak within 10 minutes, evidently
decreasing after 30 minutes. This could be explained by the
physiological and biochemical adaptation of fish to altered
environmental conditions. It has been established that the locomotor
activity of juvenile trout depends on leachate concentration and
exposure duration. Modeling of these parameters showed that the
activity of juveniles increased at higher leachate concentrations, but
slightly decreased with the increasing exposure duration. Experiment
results confirm that the behavior of rainbow trout juveniles is a
sensitive and rapid biomarker that can be used in combination with
the system for fish behavior monitoring, registration and analysis to
determine sublethal concentrations of pollutants in ambient water.
Further research should be focused on software improvement aimed
to include more parameters of aquatic organisms’ behavior and to
investigate the most rapid and appropriate behavioral responses in
different species. In practice, this study could be the basis for the
development and creation of biological early-warning systems
(BEWS).
Abstract: This study evaluated the acute toxicity and tissue
distribution of intravenously administered gold nanoparticles
(AuNPs) in male rabbits. Rabbits were exposed to single dose of
AuNPs (300 μg/ kg). Toxic effects were assessed via general
behavior, hematological parameters, serum biochemical parameters,
and histopathological examination of various rabbits’ organs.
Inductively coupled plasma–mass spectrometry (ICP-MS) was used
to determine gold concentrations in tissue samples collected at
predetermined time intervals. After one week, AuNPs exerted no
obvious acute toxicity in rabbits. However, inflammatory reactions
were observed in liver, lungs and kidneys accompanied with mild
absolute neutrophilia and significant monocytosis. The highest gold
levels were found in the spleen and liver followed by lungs, and
kidneys. These results indicated that AuNPs could be distributed
extensively to various tissues in the body, but primarily in the spleen
and liver.
Abstract: Kurtbogazi Dam has gained special meaning for Ankara, Turkey for the last decade due to the rapid depletion of nearby resources of drinking water. In this study, the results of the analyses of Kurtbogazi Dam outlet water and the rivers flowing into the Kurtbogazi Dam were discussed for the period of last five years between 2008 and 2012. Some physical and chemical properties (pH, temperature, biochemical oxygen demand (BOD5), nitrate, phosphate and chlorine) of these water resources were evaluated. They were classified according to the Council Directive (75/440/EEC). Moreover, the properties of these surface waters were assessed to determine the quality of water for drinking and irrigation purposes using Piper, US Salinity Laboratory and Wilcox diagrams. The results showed that all the water resources are acceptable level as surface water except for Pazar Stream in terms of ortho-phosphate and BOD5 concentration for 2008.
Abstract: High moisture content in fruits generates post-harvest
problems such as mechanical, biochemical, microbial and physical
losses. Dehydration, which is based on the reduction of water activity
of the fruit, is a common option for overcoming such losses.
However, regular hot air drying could affect negatively the quality
properties of the fruit due to the long residence time at high
temperature. Power ultrasound (US) application during the
convective drying has been used as a novel method able to enhance
drying rate and, consequently, to decrease drying time. In the present
study, a new approach was tested to evaluate the effect of US on the
drying time, the final antioxidant activity (AA) and the total
polyphenol content (TPC) of banana slices (BS), mango slices (MS)
and guava slices (GS). There were also studied the drying kinetics
with nine different models from which water effective diffusivities
(Deff) (with or without shrinkage corrections) were calculated.
Compared with the corresponding control tests, US assisted drying
for fruit slices showed reductions in drying time between 16.23 and
30.19%, 11.34 and 32.73%, and 19.25 and 47.51% for the MS, BS
and GS respectively. Considering shrinkage effects, Deff calculated
values ranged from 1.67*10-10 to 3.18*10-10 m2/s, 3.96*10-10 and
5.57*10-10 m2/s and 4.61*10-10 to 8.16*10-10 m2/s for the BS, MS and
GS samples respectively. Reductions of TPC and AA (as DPPH)
were observed compared with the original content in fresh fruit data
in all kinds of drying assays.
Abstract: Urinary Tract Infections are considered as one of the
most common bacterial infections with an estimated annual global
incidence of 150 million. Antimicrobial drug resistance is one of the
major threats due to wide spread usage of uncontrolled antibiotics. In
this study, a total number of 9149 urine samples were collected from
R.H Patiala and processed in the Department of Microbiology G. M.
C Patiala (January 2013 to December 2013). Urine samples were
inoculated on MacConkey’s and blood agar plates and incubated at
370C for 24 hrs. The organisms were identified by colony characters,
Gram’s staining, and biochemical reactions. Antimicrobial
susceptibility of the isolates was determined against various
antimicrobial agents (Hi – Media Mumbai India) by Kirby Bauer
DISK diffusion method on Muller Hinton agar plates. Maximum patients were in the age group of 21-30 yrs followed by
31-40 yrs. Males (34%) are less prone to urinary tract infections than
females (66%). Culture was positive in 25% of the samples.
Escherichia coli was the most common isolate 60.3% followed by
Klebsiella pneumoniae 13.5%, Proteus spp. 9% and Staphylococcus
aureus 7.6%. Most of the urinary isolates were sensitive to,
carbepenems, Aztreonam, Amikacin, and Piperacillin + Tazobactum.
All the isolates showed a good sensitivity towards Nitrofurantoin
(82%). ESBL production was found to be 70.6% in Escherichia coli
and 29.4% in Klebsiella pneumonia. Susceptibility of ESBL
producers to Imipenem, Nitrofurantoin and Amikacin were found to
be 100%, 76%, and 75% respectively. Uropathogens are increasingly
showing resistance to many antibiotics making empiric management
of outpatient UTIs challenging. Ampicillin, Cotrimoxazole and
Ciprofloxacin should not be used in empiric treatment. Nitrofurantoin
could be used in lower urinary tract infection. Knowledge of
uropathogens and their antimicrobial susceptibility pattern in a
geographical region will help in appropriate and judicious antibiotic
usage in a health care setup.
Abstract: The consumption of food contaminated with molds
(microscopic filamentous fungi) and their toxic metabolites results in
the development of food-borne mycotoxicosis. The spores of molds
are ubiquitously spread in the environment and can be detected
everywhere. Ochratoxin A is a toxic and potentially carcinogenic
fungal toxin found in a variety of food commodities. In this study, the
mycological quality of various ready-to-eat local and imported pork
meat and meat byproducts sold in Egyptian markets were assessed
and the presence of various molds was determined in pork used as a
raw material, edible organs as liver and kidney as well as in
fermented raw meat by-products. The study assessed the mycological
quality of pork raw meat and their by-products sold in commercial
shops in Cairo, Egypt. Mycological analysis was conducted on
(n=110) samples which included pig’s livers and kidneys from
Egyptian Bassatin slaughter house; local and imported processed
pork meat by-products from Egyptian pork markets. The isolates
were identified using traditional mycological and biochemical tests.
All kidney and liver samples were positive to molds growth while all
byproducts were negative. Ochratoxin A levels were quantitatively
analyzed using the high performance liquid chromatography (HPLC)
and the highest results were present in kidney 7.51 part per billion
(ppb) followed by minced meat 6.19 ppb generally the local samples
showed higher levels than the imported ones. To the best of our
knowledge, this is the first report on mycotoxins detection and
quantification from pork by-products in Egypt.
Abstract: The quantitative study of cell mechanics is of
paramount interest, since it regulates the behaviour of the living cells
in response to the myriad of extracellular and intracellular
mechanical stimuli. The novel experimental techniques together with
robust computational approaches have given rise to new theories and
models, which describe cell mechanics as combination of
biomechanical and biochemical processes. This review paper
encapsulates the existing continuum-based computational approaches
that have been developed for interpreting the mechanical responses of
living cells under different loading and boundary conditions. The
salient features and drawbacks of each model are discussed from both
structural and biological points of view. This discussion can
contribute to the development of even more precise and realistic
computational models of cell mechanics based on continuum
approaches or on their combination with microstructural approaches,
which in turn may provide a better understanding of
mechanotransduction in living cells.
Abstract: This study aimed to determine the possible protective effects of L‐carnitine against gentamicin‐induced nephrotoxicity. Forty male albino rats were divided into 4 groups (10 rats each); Group 1: normal control, group 2: induced nephrotoxicity (gentamicin 50 mg/kg/day S.C; 8 days), group 3: treated with L‐ carnitine (40 mg/kg/d SC for 12 days) and group 4: treated with L‐ carnitine 4 days before and for 8 days in concomitant with gentamicin. Gentamicin‐induced nephrotoxicity (group 2): caused significant increase in serum urea, creatinine, urinary N‐acetyl‐B‐D‐ glucosaminidase (NAG), gamma glutamyl transpeptidase (GGT), urinary total protein and kidney tissue malondialdehyde (MDA) with significant decrease in serum superoxide dismutase (SOD), serum catalase and creatinine clearance and marked tubular necrosis in the proximal convoluted tubules with interruption in the basement membrane around the necrotic tubule compared to the normal control group. L‐carnitine 4 days before and for 8 days in concomitant with gentamicin (group 4) offered marked decrease in serum urea, serum creatinine, urinary NAG, urinary GGT, urinary proteins and kidney tissue MDA, with marked increase in serum SOD, serum catalase and creatinine clearance with marked improvement in the tubular damage compared to gentamicin‐induced nephrotoxicity group. L‐carnitine administered for 12 days produced no change in the parameters mentioned above as compared to the normal control group. In conclusion: L‐carnitine could reduce most of the biochemical parameters and also improve the histopathological features of kidney asscociated with gentamicin induced‐nephrotoxicity.
Abstract: Current research is targeting new molecular
mechanisms that underlie non-alcoholic fatty liver disease (NAFLD)
and associated metabolic disorders like non-alcoholic steatohepatitis
(NASH). Forty New Zealand White rabbits have been used and fed a
high protein (HP) and energy diet based on grains and containing
11.76 MJ/kg. Boron added to 3 experimental groups’ drinking waters
(30 mg boron/L) as boron compounds. Biochemical analysis
including boron levels, and nuclear magnetic resonance (NMR) based
metabolomics evaluation, and mRNA expression of peroxisome
proliferator-activated receptor (PPAR) family was performed. LDLcholesterol
concentrations alone were decreased in all the
experimental groups. Boron levels in serum and feces were increased.
Content of acetate was in about 2x higher for anhydrous borax group,
at least 3x higher for boric acid group. PPARα mRNA expression
was significantly decreased in boric acid group. Anhydrous borax
attenuated mRNA levels of PPARγ, which was further suppressed by
boric acid. Boron supplementation decreased the degenerative
alterations in hepatocytes. Except borax group other boron groups did
not have a pronounced change in tubular epithels of kidney. In
conclusion, high protein and energy diet leads hepatocytes’
degenerative changes which can be prevented by boron
supplementation. Boric acid seems to be more effective in this
situation.
Abstract: This paper shortly describes various types of biomass
and a growing number of facilities utilizing the biomass in the Czech
Republic. The considerable part of this paper deals with energy
parameters of the most frequently used types of biomass and results
of their gasification testing. Sixteen most used "Czech" woody plants
and grasses were selected; raw, element and biochemical analyses
were performed and basic calorimetric values, ash composition, and
ash characteristic temperatures were identified. Later, each biofuel
was tested in a fluidized bed gasifier. The essential part of this paper
provides results of the gasification of selected biomass types.
Operating conditions are described in detail with a focus on
individual fuels properties. Gas composition and impurities content
are also identified. In terms of operating conditions and gas quality,
the essential difference occurred mainly between woody plants and
grasses. The woody plants were evaluated as more suitable fuels for
fluidized bed gasifiers. Testing results significantly help with a
decision-making process regarding suitability of energy plants for
growing and with a selection of optimal biomass-treatment
technology.
Abstract: In review the generalized data about biosynthetic routs
formation anthraquinone molecules in natural cells. The basic
possibilities of various ways of biosynthesis of different quinoid
substances are shown.
Abstract: Enterococci are important inhabitants of the animal
intestine and are widely used in probiotic products. A probiotic strain
is expected to possess several desirable properties in order to exert
beneficial effects. Therefore, the objective of this study was to
isolate, characterize and identify Enterococcus sp. from chicken cecal
and fecal samples to determine potential probiotic properties.
Enterococci were isolated from chicken ceca and feces of thirty three
clinically healthy chickens from a local farm. In vitro studies were
performed to assess antibacterial activity of the isolated LAB (using
agar well diffusion and cell free supernatant broth technique against
Salmonella enterica serotype Enteritidis), survival in acidic
conditions, resistance to bile salts, and their survival during simulated
gastric juice conditions at pH 2.5. Isolates were identified by
biochemical carbohydrate fermentation patterns using an API 50
CHL kit and API ZYM kits and by sequenced 16S rDNA. An isolate
belonging to E. faecium species exhibited inhibitory effect against S.
enteritidis. This isolate producing a clear zone as large as 10.30 mm
or greater and was able to grow in the coculture medium and at the
same time, inhibited the growth S. enteritidis. In addition, E. faecium
exhibited significant resistance under highly acidic conditions at pH
2.5 for 8 h and survived well in bile salt at 0.2% for 24 h and showing
ability to survive in the presence of simulated gastric juice at pH 2.5.
Based on these results, E. faecium isolate fulfills some of the criteria
to be considered as a probiotic strain and therefore, could be used as a
feed additive with good potential for controlling S. Enteritidis in
chickens. However, in vivo studies are needed to determine the safety
of the strain.
Abstract: The rhizome of Java grass, Cyperus rotundus was
extracted different organic polar and non-polar solvents and
performed the in vitro antiviral and immunostimulant activities
against White Spot Syndrome Virus (WSSV) and Vibrio harveyi
respectively. Based on the initial screening the ethyl acetate extract of
C. rotundus was strong activities and further it was purified through
silica column chromatography and the fractions were screened again
for antiviral and immunostimulant activity. Among the different
fractions screened against the WSSV and V. harveyi, the fractions, FIII
to FV had strong activities. In order to study the in vivo influence
of C. rotundus, the fractions (F-III to FV) were pooled and delivered
to the F. indicus through artificial feed for 30 days. After the feeding
trail the experimental and control diet fed F. indicus were challenged
with virulent WSSV and studied the survival, molecular diagnosis,
biochemical, haematological, and immunological parameters.
Surprisingly, the pooled fractions (F-IV to FVI) incorporated diets
helped to significantly (P
Abstract: The effects of basil and/or chamomile seed
supplementation on the growth of Hubbard broiler chicks were
evaluated. The antioxidant effects of these supplements were also
assessed. 120 1-day-old broiler chicks were randomly divided into
four equal groups. The control group (group 1) was fed a basal diet
(BD) without supplementation. Groups 2, 3, and 4 were fed the BD
supplemented with 10g basil, 10g chamomile, and 5g basil plus 5g
chamomile per kg of food, respectively. Basil supplementation alone
or in combination with chamomile non-significantly (P≥0.05)
increased final body weight (3.2% and 0.3%, respectively) and
weight gain (3.5% and 3.6%, respectively) over the experimental
period. Chamomile supplementation alone non-significantly (P≥0.05)
reduced final body weight and weight gain over the experimental
period by 1.7% and 1.7%, respectively. In comparison to the control
group, herbal seed supplementation reduced feed intake and
improved the feed conversion and protein efficiency ratios. In
general, basil seed supplementation stimulated chicken growth and
improved the feed efficiency more effectively than chamomile seed
supplementation. The antioxidant activities of basil and/or chamomile
supplementation were examined in the thymus, bursa, and spleen. In
chickens that received supplements, the level of malondialdehyde
was significantly decreased, whereas the activities of glutathione,
superoxide dismutase, and catalase were significantly increased
(P