Abstract: Some of the main problems man contends with are the quantity (source and amount) and quality of water in Nigeria. Scarcity leads to water being obtained from various sources and microbiological contamination of the water may thus occur between the collection point and the point of usage. This study thus aims to assess the general and microbiological quality of domestic water sources and household stored water used within selected areas in Ile-Ife, South-Western part of Nigeria for microbial contaminants. Physicochemical and microbiological examination were carried out on 45 source and stored water samples collected from well and spring in three different local government areas i.e. Ife east, Ife-south and Ife-north. Physicochemical analysis included pH value, temperature, total dissolved solid, dissolved oxygen and biochemical oxygen demand. Microbiology involved most probable number analysis, total coliform, heterotrophic plate, faecal coliform and streptococcus count.
The result of the physicochemical analysis of samples showed anomalies compared to acceptable standards with the pH value of 7.20-8.60 for stored and 6.50-7.80 for source samples. The total dissolved solids (TDS of stored 20-70mg/L, source 352-691mg/L), dissolved oxygen (DO of stored 1.60-9.60mg/L, source 1.60-4.80mg/L), biochemical oxygen demand (BOD stored 0.80-3.60mg/L, source 0.60-5.40mg/L). General microbiological quality indicated that both stored and source samples with the exception of a sample were not within acceptable range as indicated by analysis of the MPN/100ml which ranges between (stored 290-1100mg/L, source 9-1100mg/L). Apart from high counts, most samples did not meet the World Health Organization standard for drinking water with the presence of some pathogenic bacteria and fungi such as Salmonella and Aspergillus spp. To annul these constraints, standard treatment methods should be adopted to make water free from contaminants. This will help identify common and likely water related infection origin within the communities and thus help guide in terms of interventions required to prevent the general populace from such infections.
Abstract: Currently, mathematical and computer modeling are widely used in different biological studies to predict or assess behavior of such a complex systems as a biological are. This study deals with mathematical and computer modeling of bi-substrate enzymatic reactions, which play an important role in different biochemical pathways. The main objective of this study is to represent the results from in silico investigation of bi-substrate enzymatic reactions in the presence of uncompetitive inhibitors, as well as to describe in details the inhibition effects. Four models of uncompetitive inhibition were designed using different software packages. Particularly, uncompetitive inhibitor to the first [ES1] and the second ([ES1S2]; [FS2]) enzyme-substrate complexes have been studied. The simulation, using the same kinetic parameters for all models allowed investigating the behavior of reactions as well as determined some interesting aspects concerning influence of different cases of uncompetitive inhibition. Besides, it has been shown that uncompetitive inhibitors exhibit specific selectivity depending on mechanism of bi-substrate enzymatic reaction.
Abstract: Introduction: Pegylated Interferon and Ribavirin combination is standard of care in the management of chronic HCV infected patients. Efficacy of the therapy is judged by the ability to achieve biochemical and virological response as judged by RVR, EVR, ETR and SVR.Objective: To evaluate the efficacy of newly marketed biosimilar Pegylated Interferon Alpha 40KD (Peg INF) in chronic HCV patients. Materials and methods: This was observational, prospective multicentre study to evaluate the ability of biosimilar pegylated interferon alfa 2a (40KD) along with Ribavirin (weight based) to achieve SVR. The enrolled patients were separated into Naïve (A), Relapsers (B) and Non Responders(C) based on the previous history of interferon exposure and its response. The RGT was followed on ALT and RVR, EVR, ETR and SVR.Results:As per protocol analysis estimated SVR for three groups is 86.6% for naïve, 89.4% for relapsers and 52.4% for non-responders to standard interferon. Conclusion: It is concluded that Bio-similar pegylated interferon alfa-2a (40kD) along with Ribavirin has good anti-viral efficacy in Naïve, Relapsers and Non-responders to standard IFN of chronic HCV infected patients requiring treatment.
Abstract: The degrading effect due to bacterial growth on the structural integrity of concrete floor surfaces is predictable; this consequently cause development of surface micro cracks in which organisms penetrate through resulting in surface spalling. Hence, the need to develop mix design meeting the requirement of floor surfaces exposed to aggressive agent to improve certain material properties with good workability, extended lifespan and low cost is essential. In this work, tests were performed to examine the microbial activity on kitchen floor surfaces and the effect of adding admixtures. The biochemical test shows the existence of microorganisms (E.coli, Streptococcus) on newly casted structure. Of up to 6% porosity was reduced and improvement on structural integrity was observed upon adding mineral admixtures from the concrete mortar. The SEM result after 84 days of curing specimens, shows that chemical admixtures have significant role to enable retard bacterial penetration and good quality structure is achieved.
Abstract: This study was conducted to evaluate the anti-diabetic
properties of ethanolic extract of two plants commonly used in folk
medicine, Mormodica charantia (bitter melon) and Trigonella
foenum-graecum (fenugreek). The study was performed on STZinduced
diabetic rats (DM type-I). Plant extracts of these two plants
were given to STZ diabetic rats at the concentration of 500 mg/kg
body weight ,50 mg/kg body weight respectively. Cidophage®
(metformin HCl) were administered to another group to support the
results at a dose of 500 mg/kg body weight, the ethanolic extracts and
Cidophage administered orally once a day for four weeks using a
stomach tube and; serum samples were obtained for biochemical
analysis. The extracts caused significant decreases in glucose levels
compared with diabetic control rats. Insulin secretions were increased
after 4 weeks of treatment with Cidophage® compared with the
control non-diabetic rats. Levels of AST and ALT liver enzymes were
normalized by all treatments. Decreases in liver cholesterol,
triglycerides, and LDL in diabetic rats were observed with all
treatments. HDL levels were increased by the treatments in the
following order: bitter melon, Cidophage®, and fenugreek. Creatinine
levels were reduced by all treatments. Serum nitric oxide and
malonaldehyde levels were reduced by all extracts. GSH levels were
increased by all extracts. Extravasation as measured by the Evans
Blue test increased significantly in STZ-induced diabetic animals.
This effect was reversed by ethanolic extracts of bitter melon or
fenugreek.
Abstract: An overview of the important aspects of managing
and controlling industrial effluent discharges to public sewers namely
sampling, characterization, quantification and legislative controls has
been presented. The findings have been validated by means of a case
study covering three industrial sectors namely, tanning, textile
finishing and food processing industries. Industrial effluents
discharges were found to be best monitored by systematic and
automatic sampling and quantified using water meter readings
corrected for evaporative and consumptive losses. Based on the
treatment processes employed in the public owned treatment works
and the chemical oxygen demand and biochemical oxygen demand
levels obtained, the effluent from all the three industrial sectors
studied were found to lie in the toxic zone. Thus, physico-chemical
treatment of these effluents is required to bring them into the
biodegradable zone. KL values (quoted to base e) were greater than
0.50 day-1 compared to 0.39 day-1 for typical municipality
wastewater.
Abstract: Microbial-induced calcite precipitation (MICP) is a
relatively green and sustainable soil improvement technique. It
utilizes biochemical process that exists naturally in soil to improve
engineering properties of soils. The calcite precipitation process is
uplifted by the mean of injecting higher concentration of urease
positive bacteria and reagents into the soil. The main objective of this
paper is to provide an overview of the factors affecting the MICP in
soil. Several factors were identified including nutrients, bacteria type,
geometric compatibility of bacteria, bacteria cell concentration,
fixation and distribution of bacteria in soil, temperature, reagents
concentration, pH, and injection method. These factors were found to
be essential for promoting successful MICP soil treatment.
Furthermore, a preliminary laboratory test was carried out to
investigate the potential application of the technique in improving the
shear strength and impermeability of a residual soil specimen. The
results showed that both shear strength and impermeability of
residual soil improved significantly upon MICP treatment. The
improvement increased with increasing soil density.
Abstract: Gastric ulceration is a discontinuity in gastric mucosa, usually occurs due to imbalance between the gastric mucosal protective factors, that is called gastric mucosal barrier, and the aggressive factors, to which the mucosa is exposed. This study was carried out on sixty male Sprague-Dowely rats (12- 16 weeks old) allocated into two groups. The first control group and the second Gastric lesion group which induced by oral administration of a single daily dose of aspirin at a dose of 300 mg/kg body weight for 7 consecutive-days (6% aspirin solution will be prepared and each rat will be given 5 ml of that solution/kg body weight). Blood is collected 1, 2 and 3 weeks after induction of gastric ulceration. Significant increase in serum copper, nitric oxide, and prostaglandin E2 all over the period of experiment. Significant decrease in erythrocyte superoxide dismutase (t-SOD) activities, serum (calcium, phosphorus, glucose and insulin) levels. Non-significant changes in serum sodium and potassium levels are obtained.
Abstract: The experimental design was 4 x 5 factorial with three
replications in fully controlled research greenhouse in Department of
Soil Sciences and Plant Nutrition, Faculty of Agriculture, University
of Selcuk in the year of 2009. Determination of tolerant chickpea
genotypes to drought was made in the research. Additionally,
sophisticated effects of drought on plant growth and development,
biochemical and physical properties or physical defense mechanisms
were presented. According to the results, the primary genotypes were
Ilgın YP (0.0063 g/gh) for leaf water capacity, 22235 70.44(%) for
relative water content, 22159 (82.47%) for real water content,
22159 (5.03 mg/l) for chlorophyll a+b, Ilgın YP (125.89 nmol
H2O2.dak-1/ mg protein-1) for peroxidase, Yunak YP (769.67
unit/ mg protein-1) for superoxide dismutase, Seydişehir YP
(16.74 μg.TA-1) for proline, Gökçe (80.01 nmol H2O2.dak-1/ mg
protein-1) for catalase. Consequently, all the genotypes
increased their enzyme activity depending on the increasing of
drought stress consider with the effects of drought stress on leaf
enzyme activity. Chickpea genotypes are increasing enzyme
activity against to drought stress.
Abstract: Inflammatory bowel disease (IBD) is a chronic
relapsing-remitting condition that afflicts millions of people
throughout the world and impairs their daily functions and quality of
life. Treatment of IBD depends largely on 5-aminosalicylic acid (5-
ASA) and corticosteroids. The present study aimed to clarify the
effects of 5-aminosalicylic acid, budesonide and currcumin on 90
male albino rats against trinitrobenzene sulfonic acid (TNB) induced
colitis. TNB was injected intrarectally to 50 rats. The other 40 rats
served as control groups. Both 5-ASA (in a dose of 120 mg/kg) and
budesonide (in a dose of 0.1 mg/kg) were administered daily for one
week whereas currcumin was injected intraperitonially (in a dose of
30 mg/kg daily) for 14 days after injection of either TNB in the
colitis rats (group B) or saline in control groups (group A). The study
included estimation of macroscopic score index, histological
examination of H&E stained sections of the colonic tissue,
biochemical estimation of myeloperoxidase (MPO), nitric oxide
(NO), and caspase-3 levels, in addition to studying the effect of tested
drugs on colonic motility. It was found that budesonide and curcumin
improved mucosal healing, reduced both NO production and caspase-
3 level. They had the best impact on the disturbed colonic motility in
TNBS-model of colitis.
Abstract: A total of twenty tensile biopsies were collected from
children undergoing tonsillectomy from teaching hospital ENT
department and Kurdistan private hospital in sulaimani city. All
biopsies were homogenized and cultured; the obtained bacterial
isolates were purified and identified by biochemical tests and VITEK
2 compact system. Among the twenty studied samples, only one
Pseudomonas putida with probability of 99% was isolated.
Antimicrobial susceptibility was carried out by disk diffusion
method, Pseudomonas putida showed resistance to all antibiotics
used except vancomycin. The isolate further subjected to PCR and
DNA sequence analysis of blaVIM gene using different set of primers
for different regions of VIM gene. The results were found to be PCR
positive for the blaVIM gene. To determine the sequence of blaVIM
gene, DNA sequencing performed. Sequence alignment of blaVIM
gene with previously recorded blaVIM gene in NCBI- database showed
that P. putida isolate have different blaVIM gene.
Abstract: This study was conducted to determine effect of water stress on chlorophyll content and chlorophyll fluorescence parameter in young `Dezful- olive trees. Three irrigation regimes (40% ETcrop, 65% ETcrop and 100% ETcrop) were used. After irrigation treatments were applied, some of biochemical parameters including chlorophyll a, b, total chlorophyll, chlorophyll fluorescence and also chlorophyll content index (C.C.I) were measured. Results of Analysis of variance showed that irrigation treatments had significant effect on chlorophylla, total chlorophyll (chl a+b), C.C.I and Fv/Fm ratio. The amount of decreased chlorophyll a and total chlorophyll in plants were received 40% ETcrop were 51.55% and 46.86%, respectively, compared with 100% ETcrop.
Abstract: Modeling and simulation of biochemical reactions is of great interest in the context of system biology. The central dogma of this re-emerging area states that it is system dynamics and organizing principles of complex biological phenomena that give rise to functioning and function of cells. Cell functions, such as growth, division, differentiation and apoptosis are temporal processes, that can be understood if they are treated as dynamic systems. System biology focuses on an understanding of functional activity from a system-wide perspective and, consequently, it is defined by two hey questions: (i) how do the components within a cell interact, so as to bring about its structure and functioning? (ii) How do cells interact, so as to develop and maintain higher levels of organization and functions? In recent years, wet-lab biologists embraced mathematical modeling and simulation as two essential means toward answering the above questions. The credo of dynamics system theory is that the behavior of a biological system is given by the temporal evolution of its state. Our understanding of the time behavior of a biological system can be measured by the extent to which a simulation mimics the real behavior of that system. Deviations of a simulation indicate either limitations or errors in our knowledge. The aim of this paper is to summarize and review the main conceptual frameworks in which models of biochemical networks can be developed. In particular, we review the stochastic molecular modelling approaches, by reporting the principal conceptualizations suggested by A. A. Markov, P. Langevin, A. Fokker, M. Planck, D. T. Gillespie, N. G. van Kampfen, and recently by D. Wilkinson, O. Wolkenhauer, P. S. Jöberg and by the author.
Abstract: Thyroid dysfunction is one of the most frequently
reported complications of chronic blood transfusion therapy in patients with beta-thalassemia major (BTM). However, the occurrence of thyroid dysfunction and its possible association with
iron overload in BTM patients is still under debate. Therefore, this
study aimed to investigate the status of thyroid functions and iron overload in adolescent and young adult patients with BTM in Jordan population. Thirty six BTM patients aged 12-28 years and matched controls were included in this study. All patients have been receiving frequent blood transfusion to maintain pretransfusion hemoglobin
concentration above 10 g dl-1 and deferoxamine at a dose of 45 mg kg-1 day-1 (8 h, 5-7 days/week) by subcutaneous infusion. Blood
samples were drawn from patients and controls. The status of thyroid functions and iron overload was evaluated by measurements of serum
free thyroxine (FT4), triiodothyronine (FT3), thyrotropin (TSH) and
serum ferritin level. A number of some hematological and
biochemical parameters were also measured. It was found that hematocrit, serum ferritin, hemoglobin, FT3 and zinc, copper mean values were significantly higher in the patients than in the controls (P< 0.05). On other hand, leukocyte, FT4 and TSH mean values were
similar to that of the controls. In addition, our data also indicated that
all of the above examined parameters were not significantly affected
by the patient-s age and gender. Deferoxamine approach for removing excess iron from our BTM patient did not normalize the
values of serum ferritin, copper and zinc, suggesting poor compliance
with deferoxamine chelation therapy. Thus, we recommend the use
of a combination of deferoxamine and deferiprone to reduce the risk
of excess of iron in our patients. Furthermore, thyroid dysfunction
appears to be a rare complication, because our patients showed
normal mean levels for serum TSH and FT4. However, high mean
levels of serum ferritin, zinc, copper might be seen as potential risk
factors for initiation and development of thyroid dysfunctions and
other diseases. Therefore, further studies must be carried out at
yearly intervals with large sample number, to detect subclinical
thyroid dysfunction cases.
Abstract: This study aims to initiate sustainable water management for tourist accommodations in Amphawa, Samut Songkram Province, Thailand. Wastewater generated by tourist accommodation was conducted in 10 homestays and resorts in Amphawa during August – October, 2011. The prominent parameters which are of pH, Dissolved Oxygen (DO), Biochemical Oxygen Demand (BOD), Fat Oil and Grease (FOG), Nitrate (No3-), and Phosphate (PO43-) were conducted monthly. The results revealed that some parameters were over national water quality standard (Class II). Especially, 90% of tourist accommodations have been recorded that FOG was over the standard of wastewater quality from accommodation (group I: total room of accommodation less than 200 rooms). Therefore, grease trap and natural treatment should be utilized in tourist accommodations in order to reduce the discharged of fat, oil, and grease from tourism activities. In addition, number of tourists also relate statistically with BOD and Nitrate at 0.05 level of significance.
Abstract: Sickle cell anemia is a recessive genetic disease
caused by the presence in the red blood cell, of abnormal hemoglobin
called hemoglobin S. It results from the replacement in the beta chain
of the acid glutamic acid by valin at position 6. Topics may be
homozygous (SS) or heterozygous (AS) most often
asymptomatic. Other mutations result in compound heterozygous:
- Synthesis of hemoglobin C mutation in the sixth leucin codon
(heterozygous SC);
- ß-thalassemia (heterozygous S-ß thalassemia).
SS homozygous, heterozygous SC and S- ß -thalassemia are grouped
under the major sickle cell syndromes.
To make a laboratory diagnosis of hemoglobinopathies in a
portion of the population in region of Batna, our study was
conducted on 115 patients with suspected sickle cell anemia, all cases
have benefited from hematological tests as blood count (count RBC,
calculated erythrocyte indices, MCV and MCHC, measuring the
hemoglobin concentration) and a biochemical test in this case
electrophoresis CAPILLARYS HEMOGLOBIN (E).
The results showed:
27 cases of sickle cell anemia were found on 115 suspected cases,
73,03% homozygous sickle cell disease and 59,25% sickle cell trait.
Finally, the double heterozygous S/C, represent the incidence rate of
3, 70%.
Abstract: The Egyptian Bacillus thuringiensis isolate (M5) produce crystal proteins that is toxic against insects was irradiated with UV light to induce mutants. Upon testing 10 of the resulting mutants for their toxicity against cotton leafworm larvae, the three mutants 62, 64 and 85 proved to be the most toxic ones. Upon testing these mutants along with their parental isolate by SDS-PAGE analysis of spores-crystals proteins as well as vegetative cells proteins, new induced bands appeared in the three mutants by UV radiation and also they showed disappearance of some other bands as compared with the wild type isolate. Multiplex PCR technique, with five sets of specific primers, was used to detect the three types of cryI genes cryIAa, cryIAb and cryIAc. Results showed that these three genes exist, as distinctive bands, in the wild type isolate (M5) as well as in mutants 62 and 85, while the mutant 64 had two distinctive bands of cryIAb and cryIAc genes, and a faint band of cryI Aa gene. Finally, these results revealed that mutant 62 is considered as the promising mutant since it is UV resistant, highly toxic against Spodoptera littoralis and active against a wide range of Lepidopteran insects.
Abstract: This study was conducted to evaluate the effects of
gibberellic acid and 2,4- dichlorophenoxyacetic acid on flower
number, fruit growth and fruit quality of wax apple. GA3 and 2,4-D
were applied at small bud and petal fall stage. Number of flower,
fruit set, fruit drop, fruit crack, fruit growth and fruit quality were
recorded. Results indicated that spraying with 10 ppm GA3 had the
best results in number of flower. GA3 spray at 30 ppm gave the
faster rate of fruit growth than the other treatments. Fruit set, fruit
size as well as fruit weight markedly improved by spraying 30 ppm
GA3, followed by 10 ppm GA3 compared to untreated control.
Moreover, spray GA3 at 30 ppm was the most effective and
increased total soluble solids, reduced titratable acidity and fruit
drop. On the other hand, it was noticed that with 10 ppm 2,4-D
application also enhanced the fruit growth rate, improved
physiological and biochemical characters of fruit compared to
untreated control. It was concluded that both GA3 and 2,4-D spray
have positive effects on fruit development, reduced fruit drop, fruit
crack and improved fruit quality of wax apple under field conditions.
Abstract: The shelf life of fish was extended using disinfection
properties of ozone. For this purpose, Trout specimens were exposed
to ozone in the aqueous media for two hours and their microbial
growth and biochemical properties were measured over time.
Microbial growth of ozone treated fish was significantly slower than
control sample, resulting in lower counts of bacteria. According to
the biochemical tests; ozone treatment had no negative effects on fat,
protein and humidity of fish. Peroxide and TVN (Total Volatile
Nitrogen) measurements showed that treatment by ozone increased
the trout shelf life from 4 days to 6 days. According to the sensory
analysis, no changes were observed in color or flavor of the ozone
treated trout.
Abstract: This study was designed to investigate the role of serum nitric oxide and sialic acid in the development of diabetic nephropathy as disease marker. Total 210 diabetic patients (age and sex matched) were selected followed by informed consent and divided into four groups (70 each) as I: control; II: diabetic; III: diabetic hypertensive; IV: diabetic nephropathy. The blood samples of all subjects were collected and analyzed for serum nitric oxide, sialic acid, fasting blood glucose, serum urea, creatinine, HbA1c and GFR. The BMI, systolic and diastolic blood pressures, blood glucose, HbA1c and serum sialic acid levels were high (p