Cultivation of Thymus by In Vitro And Hydroponics Combined Method
Our results showed that for the growth of qualitative
seedling and vegetative raw material of ðó. marschallianus Willd. and
T. serphyllum L. it is more profitable to use the in vitro and
hydroponics combined method. In in vitro culture it is possible to do
micro-propagation whole year with 98-99% rhizogenesis. 30000
micro-plants were obtained from one explant during 9 months.
Hydroponic conditions provide the necessary microclimate for
microplants where the survival rate without acclimatization was
93.3%. The essential oil content in hydroponic dry herb of both
species in vegetative and blossom phase was 1.3% whereas in wild
plants it was 1.2%, the content of extractive substances and vitamin
C also exceeded wild plants. Our biochemical and radiochemical
investigations indicated that the medicinal raw materials obtained
from hydroponic and wild plants of Thymus species correspond to
the demands of SPh XI, and the content of artificial radionuclides
does not exceed the MACL.
[1] R.G.Butenko Biology of Cultivated Cells of Streptophyta by In vitro
and Biotechnology on its Base, Monograph, Moscow, p. 159, 1999 (in
Russian)
[2] G.S. Davtyan Hydroponic Production Achievement for Agrochemical
Science. Yerevan, pp.352-365, 1980 (in Russian).
[3] Essential Oils Market Information Booklet, IENICA, May 2004,
www.ienica.net
[4] European Pharmacopoeia 2005. 5th edition, Strasburg-, Version 5.1
EDQM
[5] V.P.Georgievskiy, N.F. Komissarenko, S.E. Dmitruk The Biologically
Active Substances of Herbal Plants, 1990, p. 191-242 (in Russian)
[6] N.V.Kataeva, R.G.Butenko, Clonal Micro-propagation of Plants.
Moscow, p.96, 1983 (in Russian)
[7] T. Murashige, F.Skoog A Revised Medium for Rapid Growing and
Bioassay with Tobacco Tissue Culture. // Physical Plantarium, V.1 (15),
pp.473-497, 1962.
[8] N.I.Grinkevich, L.N. Safronich The Chemical Analysis of Herbal Plants.
p. 175, 1983 (in Russian)
[9] F.I.Pavlotskaya Methods of 90 Sr and other long-living isotopes
determination. In collection. Physiological-chemical methods of soil
study. p. 126, 1966 (in Russian)
[10] B.C. Prister, C.C. Zubach The using of Arsenazo III defining U in the
soils and biological objects. "Radiochemistry" 10, Issue 6, pp. 743-748,
1968 (in Russian)
[11] Red Book of Republic of Armenia. Second edition, p. 7, 2010.
[12] E.D.Sargsyan, N.N. Tambian, S.Kh. Mairapetyan Cultivation of
Lawsonia by Hydroponics and In Vitro Combined Method in Armenia.-
Transaction of International Forum on Problems of Science, Technology
& Education, Moscow, pp. 45-47, 1998 (in Russian)
[13] A.O.Sari, B.Oguz Importance of medicinal and aromatic plants in
horticultural crops of Eagean and Marmara Regions. Eagean
Agricultural Research Institute Publications, Izmir, 2000, N 98, p. 82
[14] State Pharmacopoeia USSR, 11th edition, Moscow: "Medicine--, pp.
323-325, 1990 (in Russian)
[15] The Radiation Safety Norms. Hygienic Norms, Moscow, sanitary rules
and regulations centre, Ministry of public health of Russia, p. 116, 1999
(in Russian)
[16] A.I. Yermakov, B.B. Arasimovich, M.N. Smirnova-Ikonkinova, I.K.
Murri. Methods of plants biochemical investigations. Moscow, pp. 90-
60, 1952 (in Russian)
[1] R.G.Butenko Biology of Cultivated Cells of Streptophyta by In vitro
and Biotechnology on its Base, Monograph, Moscow, p. 159, 1999 (in
Russian)
[2] G.S. Davtyan Hydroponic Production Achievement for Agrochemical
Science. Yerevan, pp.352-365, 1980 (in Russian).
[3] Essential Oils Market Information Booklet, IENICA, May 2004,
www.ienica.net
[4] European Pharmacopoeia 2005. 5th edition, Strasburg-, Version 5.1
EDQM
[5] V.P.Georgievskiy, N.F. Komissarenko, S.E. Dmitruk The Biologically
Active Substances of Herbal Plants, 1990, p. 191-242 (in Russian)
[6] N.V.Kataeva, R.G.Butenko, Clonal Micro-propagation of Plants.
Moscow, p.96, 1983 (in Russian)
[7] T. Murashige, F.Skoog A Revised Medium for Rapid Growing and
Bioassay with Tobacco Tissue Culture. // Physical Plantarium, V.1 (15),
pp.473-497, 1962.
[8] N.I.Grinkevich, L.N. Safronich The Chemical Analysis of Herbal Plants.
p. 175, 1983 (in Russian)
[9] F.I.Pavlotskaya Methods of 90 Sr and other long-living isotopes
determination. In collection. Physiological-chemical methods of soil
study. p. 126, 1966 (in Russian)
[10] B.C. Prister, C.C. Zubach The using of Arsenazo III defining U in the
soils and biological objects. "Radiochemistry" 10, Issue 6, pp. 743-748,
1968 (in Russian)
[11] Red Book of Republic of Armenia. Second edition, p. 7, 2010.
[12] E.D.Sargsyan, N.N. Tambian, S.Kh. Mairapetyan Cultivation of
Lawsonia by Hydroponics and In Vitro Combined Method in Armenia.-
Transaction of International Forum on Problems of Science, Technology
& Education, Moscow, pp. 45-47, 1998 (in Russian)
[13] A.O.Sari, B.Oguz Importance of medicinal and aromatic plants in
horticultural crops of Eagean and Marmara Regions. Eagean
Agricultural Research Institute Publications, Izmir, 2000, N 98, p. 82
[14] State Pharmacopoeia USSR, 11th edition, Moscow: "Medicine--, pp.
323-325, 1990 (in Russian)
[15] The Radiation Safety Norms. Hygienic Norms, Moscow, sanitary rules
and regulations centre, Ministry of public health of Russia, p. 116, 1999
(in Russian)
[16] A.I. Yermakov, B.B. Arasimovich, M.N. Smirnova-Ikonkinova, I.K.
Murri. Methods of plants biochemical investigations. Moscow, pp. 90-
60, 1952 (in Russian)
@article{"International Journal of Biological, Life and Agricultural Sciences:60898", author = "E. Sargsyan and A. Vardanyan and L. Ghalachyan and S. Bulgadaryan", title = "Cultivation of Thymus by In Vitro And Hydroponics Combined Method", abstract = "Our results showed that for the growth of qualitative
seedling and vegetative raw material of ðó. marschallianus Willd. and
T. serphyllum L. it is more profitable to use the in vitro and
hydroponics combined method. In in vitro culture it is possible to do
micro-propagation whole year with 98-99% rhizogenesis. 30000
micro-plants were obtained from one explant during 9 months.
Hydroponic conditions provide the necessary microclimate for
microplants where the survival rate without acclimatization was
93.3%. The essential oil content in hydroponic dry herb of both
species in vegetative and blossom phase was 1.3% whereas in wild
plants it was 1.2%, the content of extractive substances and vitamin
C also exceeded wild plants. Our biochemical and radiochemical
investigations indicated that the medicinal raw materials obtained
from hydroponic and wild plants of Thymus species correspond to
the demands of SPh XI, and the content of artificial radionuclides
does not exceed the MACL.", keywords = "Hydroponics, In vitro, Micro-propagation, Thymus", volume = "5", number = "8", pages = "472-4", }