Abstract: Five original strains of entomopathogenic bacteria
with insecticidal activity against mosquito larvae of the genera Aedes,
Culex and Anopheles have been isolated from natural conditions in
Armenia and characterized. According to morphological,
physiological and biochemical parameters, all isolates were identified
as Bacillus thuringiensis spp. israelensis (Bti). High larvicidal
activity has been showed by three strains Bti. These strains can be
recommended for industrial production of bacterial preparations.
Abstract: Acute disseminated encephalomyelitis (ADEM) has
been reported to develop after a hymenoptera sting, but its
pathogenesis is not known in detail. Myelin basic protein (MBP)-
specific T cells have been detected in the blood of patients with
ADEM, and a proportion of these patients develop multiple sclerosis
(MS). In an attempt to understand the mechanisms underlying
ADEM, molecular mimicry between hymenoptera venom peptides
and the human immunodominant MBP peptide was scrutinized,
based on the sequence and structural similarities, whether it was the
root of the disease. The results suggest that the three wasp venom
peptides have low sequence homology with the human
immunodominant MBP residues 85-99. Structural similarity analysis
among the three venom peptides and the MS-related HLA-DR2b
(DRA, DRB1*1501)-associated immunodominant MHC
binding/TCR contact residues 88-93, VVHFFK showed that
hyaluronidase residues 7-12, phospholipase A1 residues 98-103, and
antigen 5 residues 109-114 showed a high degree of similarity
83.3%, 100%, and 83.3% respectively. In conclusion, some wasp
venom peptides, particularly phospholipase A1, may potentially act
as the molecular motifs of the human 3HLA-DR2b-associated
immunodominant MBP88-93, and possibly present a mechanism for
induction of wasp sting-associated ADEM.
Abstract: Nowadays, cardiac disease is one of the most common
cause of death. Each year almost one million of angioplasty interventions and stents implantations are made all over the world.
Unfortunately, in 20-30% of cases neointimal proliferations leads to
restenosis occurring within the following period of 3-6 months. Three major factors are believed to contribute mostly to the edge
restenosis: (a) mechanical damage of the artery-s wall caused by the
stent implantation, (b) interaction between the stent and the blood constituents and (c) endothelial growth stimulation by small (lower
that 1.5 Pa) and oscillating wall shear stress. Assuming that this last actor is particularly important, a numerical model of restenosis
basing on wall shear stress distribution in the stented artery was elaborated. A numerical simulations of the development of in-stent
restenosis have been performed and realistic geometric patterns of a
progressing lumen reduction have been obtained
Abstract: Transport and logistics are the lifeblood of societies.
There is a strong correlation between overall growth in economic
activity and growth of transport. The movement of people and goods
has the potential for creating wealth and prosperity, therefore the
state of transportation infrastructure and especially the condition of
road networks is often a governmental priority. The design, building
and maintenance of national roads constitute a substantial share of
government budgets. Taking into account the magnitude and
importance of these investments, the expedience, efficiency and
sustainability of these projects are of great public interest. This paper
provides an overview of supply chain management principles applied
to road construction. In addition, road construction performance
measurement systems and ICT solutions are discussed. Road
construction in Estonia is analyzed. The authors propose the
development of a national performance measurement system for road
construction.
Abstract: Oxidative stress is considered to be the cause for onset
and the progression of type 2 diabetes mellitus (T2DM) and
complications including neuropathy. It is a deleterious process that
can be an important mediator of damage to cell structures: protein,
lipids and DNA. Data suggest that in patients with diabetes and
diabetic neuropathy DNA repair is impaired, which prevents effective
removal of lesions. Objective: The aim of our study was to evaluate
the association of the hOGG1 (326 Ser/Cys) and XRCC1 (194
Arg/Trp, 399 Arg/Gln) gene polymorphisms whose protein is
involved in the BER pathway with DNA repair efficiency in patients
with diabetes type 2 and diabetic neuropathy compared to the healthy
subjects. Genotypes were determined by PCR-RFLP analysis in 385
subjects, including 117 with type 2 diabetes, 56 with diabetic
neuropathy and 212 with normal glucose metabolism. The
polymorphisms studied include codon 326 of hOGG1 and 194, 399
of XRCC1 in the base excision repair (BER) genes. Comet assay was
carried out using peripheral blood lymphocytes from the patients and
controls. This test enabled the evaluation of DNA damage in cells
exposed to hydrogen peroxide alone and in the combination with the
endonuclease III (Nth). The results of the analysis of polymorphism
were statistically examination by calculating the odds ratio (OR) and
their 95% confidence intervals (95% CI) using the ¤ç2-tests. Our data
indicate that patients with diabetes mellitus type 2 (including those
with neuropathy) had higher frequencies of the XRCC1 399Arg/Gln
polymorphism in homozygote (GG) (OR: 1.85 [95% CI: 1.07-3.22],
P=0.3) and also increased frequency of 399Gln (G) allele (OR: 1.38
[95% CI: 1.03-1.83], P=0.3). No relation to other polymorphisms
with increased risk of diabetes or diabetic neuropathy. In T2DM
patients complicated by neuropathy, there was less efficient repair of
oxidative DNA damage induced by hydrogen peroxide in both the
presence and absence of the Nth enzyme. The results of our study
suggest that the XRCC1 399 Arg/Gln polymorphism is a significant
risk factor of T2DM in Polish population. Obtained data suggest a
decreased efficiency of DNA repair in cells from patients with
diabetes and neuropathy may be associated with oxidative stress.
Additionally, patients with neuropathy are characterized by even
greater sensitivity to oxidative damage than patients with diabetes,
which suggests participation of free radicals in the pathogenesis of
neuropathy.
Abstract: The purpose of this research was to demonstrate
prevalence of post-exposure preventive measures (PEP) after needlestick
injuries and its relationship with locus of control beliefs in a
sample of medical students. In this cross-sectional study, 300 medical
students with history of having experienced needle stick injuries
(NSI) for at least once filled in a questionnaire to determine if they
perceived themselves to be responsible and effective in preventing
blood born infections after NSI. About 38% of students did not seek
any professional consult or PEP after NSI due to lack of enough time
or access, anxiety about tests results, belief in uselessness of followup
and not being able to change destiny. These 114 students were not
different from others regarding their scores on NSI specific scale of
locus of health control. Thus, the potentiality of NSI locus of control
beliefs in predicting PEP was not seen in this study.
Abstract: Cardiovascular disease mostly in the form of atherosclerosis is responsible for 30% of all world deaths amounting to 17 million people per year. Atherosclerosis is due to the formation of plaque. The fatty plaque may be at risk of rupture, leading typically to stroke and heart attack. The plaque is usually associated with a high degree of lumen reduction, called a stenosis. The initiation and progression of the disease is strongly linked to the hemodynamic environment near the vessel wall. The aim of this study is to validate the flow of blood mimic through an arterial stenosis model with computational fluid dynamics (CFD) package. In experiment, an axisymmetric model constructed consists of contraction and expansion region that follow a mathematical form of cosine function. A 30% diameter reduction was used in this study. Particle image velocimetry (PIV) was used to characterize the flow. The fluid consists of rigid spherical particles suspended in waterglycerol- NaCl mixture. The particles with 20 μm diameter were selected to follow the flow of fluid. The flow at Re=155, 270 and 390 were investigated. The experimental result is compared with FLUENT simulated flow that account for viscous laminar flow model. The results suggest that laminar flow model was sufficient to predict flow velocity at the inlet but the velocity at stenosis throat at Re =390 was overestimated. Hence, a transition to turbulent regime might have been developed at throat region as the flow rate increases.
Abstract: Size based filtration is one of the common methods
employed to isolate circulating tumor cells (CTCs) from whole
blood. It is well known that this method suffers from isolation
efficiency to purity tradeoff. However, this tradeoff is poorly
understood. In this paper, we present the design and manufacturing
of a special rectangular slit filter. The filter was designed to retain
maximal amounts of nucleated cells, while minimizing the pressure
on cells, thereby preserving their morphology. The key parameter,
namely, input pressure, was optimized to retain the maximal number
of tumor cells, whilst maximizing the depletion of normal blood cells
(red and white blood cells and platelets). Our results indicate that for
a slit geometry of 5 × 40 μm on a 13 mm circular membrane with a
fill factor of 21%, a pressure of 6.9 mBar yields the optimum for
maximizing isolation of MCF-7 and depletion of normal blood cells.
Abstract: En bloc assumes modeling all phases of the orthostatic test with the only one mathematical model, which allows the complex parametric view of orthostatic response. The work presents the implementation of a mathematical model for processing of the measurements of systolic, diastolic blood pressure and heart rate performed on volunteers during orthostatic test. The original assumption of model hypothesis that every postural change means only one Stressor, did not complying with the measurements of physiological circulation factor-time profiles. Results of the identification support the hypothesis that second postural change of orthostatic test causes induced Stressors, with the observation of a physiological regulation mechanism. Maximal demonstrations are on the heart rate and diastolic blood pressure-time profile, minimal are for the measurements of the systolic blood pressure. Presented study gives a new view on orthostatic test with impact on clinical practice.
Abstract: Sixteen female Holstein calves allocated in three
treatments including: 1: control, 2: fed raw fiber concentrate (RFC)
for 45 days and 3: fed RFC for 90 days. RFC supplement (Vitacel®
200) was added to milk immediately before feeding (10 g/L milk).
Withers height and body weights of calves were measured monthly.
Individual dry matter intake was recorded daily. Blood samples were
taken monthly. The result showed that calves consumed RFC had
significantly greater weaning and final body weight. Treatment effect
on dry matter intake was not significant (p>0.05). Calves fed RFC
had better feed efficiency. Withers height of calves fed RFC were
taller than the control group (p
Abstract: This experiment was conducted to investigate the
effect of dietary supplementation of different levels of black seed
(Nigella sativa L.) on the performance and immune response of broiler chicks. A total 240 day-old broiler chicks were used and
randomly allotted equally into six experimental groups designated as 1, 2, 3, 4, 5 and 6 having black seed at the rate of 0, 2, 4, 6, 8 and
10 g /kg diet respectively. The study was lasted for 42 days. Average body weight, weight gain, relative growth rate, feed
conversion, antibody titer against Newcastle disease, phagocytic activity and phagocytic index, some blood parameters(GOT, GPT,
Glucose, Cholesterol, Triglyceride, Total protein, Albumen, WBCs,
RBCs, Hb and PCV), dressing percentage, weight of different body
organs, abdominal fat weight, were determined. It was found that, N. Sativa significantly improved final body weight, total body gain
and feed conversion ratio of groups 2 and 3 when compared with the control group. Higher levels of N. Sativa did not improve
growth performance of the chicks. Non significant differences were
observed for antibody titer against Newcastle virus, WBCs count,
serum GOT, glucose level, dressing %, relative liver, spleen, heart and head percentages. Lymphoid organs (Bursa and Thymus)
improved significantly with increasing N. Sativa level in all supplemented groups. Serum cholesterol, triglyceride and visible fat
% significantly decreased with Nigella sativa supplementation while
serum GPT level significantly increased with nigella sativa
supplementation.
Abstract: In elevating performance in competetive sports, an
athlete must continously train in achieving maximum
performance,but needs to pay attention to recovery therapy, that is to
recover from fatigue as well as injury.The correct recovery therapy
will assist in process of recovery and helps in the training in
achieving better performace. Binahong (Anredera cordifolia) was
proven empirically by the locals in assisting speedy recovery from an
injury.Clinical research with lab animals receiving blunt trauma
injury, microscopically shown signs of: 1) redness, 2) heatiness, 3)
swelling and, 4) lack of activity. There is also microscopic indication
of: 1) infiltration of inflame cells (migration of cells to the trauma
area), 2) Cells necrosis, 3) Congestion (as a result of dead red blood
cells), 4) uedema. On administration of Binahong for 3 days, there is
a significant drop of 5% in cell inflammation, 2% increase of
fibroblast (cell membrance) count.Conclutin: Binahong do assist in
reducing cell inflammation and increase counts of cells fibroblast.
Suggestion: In helping athlete's to recover from force injury, we need
study about Binahong's roots to inflammation cell and healing of
injuried cell.
Abstract: Expression and secretion of inflammation markers are
disturbed in obesity. Interleukin-6 reduces body fat mass. The
common G-174C polymorphism in the promoter of IL-6 gene has
been reported that effects on transcriptional regulation. The objective
was to investigate association of the common polymorphism G-174C
with obesity in Iranian population. The present study is cross
sectional association study that included 242 individuals (110 men
and 132 women). Serum IL-6 levels, C-reactive protein, fasting
blood glucose and blood lipids profile were measured .BMI and
WHR were calculated. Genotyping is carried out by PCR and RFLP.
The frequencies of G and C allele were 64.5% and 35.5%,
respectively. The G-174C polymorphism was not associated with
BMI and WHR. However in obese individual, fasting blood glucose
was significantly higher in carrier of C allele compared with the noncarrier.
The IL-6 G-174C polymorphism is not a risk factor for
obesity in Iranian population.
Abstract: Dilated cardiomyopathy (DCM) is a severe
cardiovascular disorder characterized by progressive systolic
dysfunction due to cardiac chamber dilatation and inefficient
myocardial contractility often leading to chronic heart failure.
Recently, a genome-wide association studies (GWASs) on DCM
indicate that the ZBTB17 gene rs10927875 single nucleotide
polymorphism is associated with DCM. The aim of the study was to
identify the distribution of ZBTB17 gene rs10927875 polymorphism
in 50 Slovak patients with DCM and 80 healthy control subjects
using the Custom Taqman®SNP Genotyping assays. Risk factors
detected at baseline in each group included age, sex, body mass
index, smoking status, diabetes and blood pressure. The mean age of
patients with DCM was 52.9±6.3 years; the mean age of individuals
in control group was 50.3±8.9 years. The distribution of investigated
genotypes of rs10927875 polymorphism within ZBTB17 gene in the
cohort of Slovak patients with DCM was as follows: CC (38.8%), CT
(55.1%), TT (6.1%), in controls: CC (43.8%), CT (51.2%), TT
(5.0%). The risk allele T was more common among the patients with
dilated cardiomyopathy than in normal controls (33.7% versus
30.6%). The differences in genotype or allele frequencies of ZBTB17
gene rs10927875 polymorphism were not statistically significant
(p=0.6908; p=0.6098). The results of this study suggest that ZBTB17
gene rs10927875 polymorphism may be a risk factor for
susceptibility to DCM in Slovak patients with DCM. Studies of
numerous files and additional functional investigations are needed to
fully understand the roles of genetic associations.
Abstract: The increasing interest in plant sterol enriched foods
is due to the fact that they reduce blood cholesterol concentrations
without adverse side effects. In this context, enriched foods with
phytosterols may be helpful in protecting population against
atherosclerosis and cardiovascular diseases. The aim of the present
work was to evaluate in a population of Viseu, Portugal, the
consumption habits low-fat, plant sterol-enriched yoghurt. For this
study, 577 inquiries were made and the sample was randomly
selected for people shopping in various supermarkets. The
preliminary results showed that the biggest consumers of these
products were women aged 45 to 65 years old. Most of the people
who claimed to buy these products consumed them once a day. Also,
most of the consumers under antidyslipidemic therapeutics noticed
positive effects on hypercholesterolemia.
Abstract: We have developed a computer program consisting of
6 subtests assessing the children hand dexterity applicable in the
rehabilitation medicine. We have carried out a normative study on a
representative sample of 285 children aged from 7 to 15 (mean age
11.3) and we have proposed clinical standards for three age groups
(7-9, 9-11, 12-15 years). We have shown statistical significance of
differences among the corresponding mean values of the task time
completion. We have also found a strong correlation between the task
time completion and the age of the subjects, as well as we have
performed the test-retest reliability checks in the sample of 84
children, giving the high values of the Pearson coefficients for the
dominant and non-dominant hand in the range 0.740.97 and
0.620.93, respectively.
A new MATLAB-based programming tool aiming at analysis of
cardiologic RR intervals and blood pressure descriptors, is worked
out, too. For each set of data, ten different parameters are extracted: 2
in time domain, 4 in frequency domain and 4 in Poincaré plot
analysis. In addition twelve different parameters of baroreflex
sensitivity are calculated. All these data sets can be visualized in time
domain together with their power spectra and Poincaré plots. If
available, the respiratory oscillation curves can be also plotted for
comparison. Another application processes biological data obtained
from BLAST analysis.
Abstract: We develop new nonlinear methods of
immunofluorescence analysis for a sensitive technology of
respiratory burst reaction of DNA fluorescence due to oxidative
activity in the peripheral blood neutrophils. Histograms in flow
cytometry experiments represent a fluorescence flashes frequency as
functions of fluorescence intensity. We used the Shannon-Weaver
index for definition of neutrophils- biodiversity and Hurst index for
definition of fractal-s correlations in immunofluorescence for
different donors, as the basic quantitative criteria for medical
diagnostics of health status. We analyze frequencies of flashes,
information, Shannon entropies and their fractals in
immunofluorescence networks due to reduction of histogram range.
We found the number of simplest universal correlations for
biodiversity, information and Hurst index in diagnostics and
classification of pathologies for wide spectra of diseases. In addition
is determined the clear criterion of a common immunity and human
health status in a form of yes/no answers type. These answers based
on peculiarities of information in immunofluorescence networks and
biodiversity of neutrophils. Experimental data analysis has shown the
existence of homeostasis for information entropy in oxidative activity
of DNA in neutrophil nuclei for all donors.
Abstract: Von Willebrand-s disease is the most common
inherited bleeding disorder in humans, it
caused by qualitative abnormalities of the von Willebrand factor
(vWF). Our objective is to determine the prevalence of this disease at
part of the Algerian population in the East and the South by a
biological diagnosis based on specific biological tests (automated
platelet count, the bleeding time (TS), the time of cephalin + activator
(TCA), measure of the prothrombin rate (TP), vWF rate and factor
VIII rate, Molecular electrophoresis of vWF multimers in agarose gel
in the presence of SDS). Four patients of type III or severe
Willebrand-s disease were found on 200 suspect cases. All cases are
showed a deficit in vWF rate (< 5%), and factor VIII (P
Abstract: Automatic determination of blood in less bright or
noisy capsule endoscopic images is difficult due to low S/N ratio.
Especially it may not be accurate to analyze these images due to the
influence of external disturbance. Therefore, we proposed detection
methods that are not dependent only on color bands. In locating
bleeding regions, the identification of object outlines in the frame and
features of their local colors were taken into consideration. The results
showed that the capability of detecting bleeding was much improved.
Abstract: Hepatitis C is an infectious disease transmitted by
blood and due to hepatitis C virus (HCV), which attacks the liver.
The infection is characterized by liver inflammation (hepatitis) that is
often asymptomatic but can progress to chronic hepatitis and later
cirrhosis and liver cancer. Our problem tends to highlight on the one
hand the prevalence of infectious disease in the population of the
region of Batna and on other hand the biological characteristics of
this disease by a screening and a specific diagnosis based on
serological tests, liver checkup (measurement of haematological and
biochemical parameters).
The results showed:
The serology of hepatitis C establishes the diagnosis of infection
with hepatitis C. In this study and with the serological test, 24 cases
of the disease of hepatitis C were found in 1000 suspected cases (7
cases with normal transaminases and 17 cases with elevated
transaminases). The prevalence of this disease in this study
population was 2.4%.
The presence of hepatitis C disrupts liver function including the
onset of cytolysis, cholestasis, jaundice, thrombocytopenia, and
coagulation disorders.