Abstract: In article the data of acute toxicity for pre-clinical
researches of Ramon preparation is described. Ramon effects to
clinical characteristics of blood, cardio-vascular system, hepatotoxic
and diuretic effects were studied.
Abstract: This study was designed to investigate the role of serum nitric oxide and sialic acid in the development of diabetic nephropathy as disease marker. Total 210 diabetic patients (age and sex matched) were selected followed by informed consent and divided into four groups (70 each) as I: control; II: diabetic; III: diabetic hypertensive; IV: diabetic nephropathy. The blood samples of all subjects were collected and analyzed for serum nitric oxide, sialic acid, fasting blood glucose, serum urea, creatinine, HbA1c and GFR. The BMI, systolic and diastolic blood pressures, blood glucose, HbA1c and serum sialic acid levels were high (p
Abstract: Organochlorine pesticides (OCPs) are known to be
persistent and bioaccumulative toxicants that may cause reproductive
impairments in wildlife as well as human. The current study uses the
snail-eating turtle Malayemys macrocephala, a long-lived animal
commonly distribute in rice field habitat in central part of Thailand,
as a sentinel to monitor OCP contamination in environment. The
nest soil, complete clutch of eggs, and blood of the turtle were
collected from agricultural areas in the Chao Phraya River Basin,
Thailand during the nesting season of 2007-2008. The novel
methods for tissue extraction by an accelerated solvent extractor
(ASE, for egg) and liquid-liquid extraction (for blood) have been
developed. The nineteen OCP residues were analyzed by gas
chromatography with micro-electron captured detector (GC-μECD).
The validated methods have met requirements of the AOAC
standard. The results indicated that significant amounts of OCPs are
still contaminated in nest soil and eggs of the turtle even though the
OCPs had been banned in this area for many years. This suggested
the potential risk to health of wildlife as well as human in the area.
Abstract: This research studied the hypoglycemic effect of
water soluble polysaccharide (WSP) extracted from yam (Dioscorea
hispida) tuber by three different methods: aqueous extraction, papain
assisted extraction, and tempeh inoculums assisted extraction. The
two later extraction methods were aimed to remove WSP binding
protein to have more pure WSP. The hypoglycemic activities were
evaluated by means in vivo test on alloxan induced hyperglycemic
rats, glucose response test (GRT), in situ glucose absorption test
using everted sac, and short chain fatty acids (SCFAs) analysis. All
yam WSP extracts exhibited ability to decrease blood glucose level in
hyperglycemia condition as well as inhibited glucose absorption and
SCFA formation. The order of hypoglycemic activity was tempeh
inoculums assisted- >papain assisted- >aqueous WSP extracts. GRT
and in situ glucose absorption test showed that order of inhibition
was papain assisted- >tempeh inoculums assisted- >aqueous WSP
extracts. Digesta of caecum of yam WSP extracts oral fed rats had
more SCFA than control. Tempeh inoculums assisted WSP extract
exhibited the most significant hypoglycemic activity.
Abstract: The adverse effects of Clindamycin (Clind.) /
Ibuprofen (Ibu.) combination on liver, kidney, blood elements and the
significances of antioxidants (N-acetylcysteine and Zinc) against
these effects were evaluated. The study includes: Group I; control
n=30, Group II; patients on Clind.300mg/Ibu.400mg twice daily for a
week n=30, Group III; patients on Clind.300mg/Ibu.400mg+Nacetylcysteine
200mg twice daily for a week n=15 and Group IV;
patients on Clind.300mg/Ibu.400mg+Zinc50mg twice daily for a
week n=15. Serum malondialdehyde (MDA), alanine transferase
(ALT), aspartate transferase (AST), γ glutamyl transferase (GGT),
creatinine, blood urea nitrogen (BUN) were measured. Applying one
way ANOVA followed by Tuckey Kramer post test, Group II showed
significant increase in ALT, AST, GGT, BUN and decrease in Hb,
RBCs, platelets than Group I. Group III showed significant decrease
in ALT, AST, GGT, BUN than Group II. Moreover, Group IV
showed significant decrease in ALT, AST, GGT and increase in Hb,
RBCs, and platelets than Group II. Conclusively, Adding Zinc or Nacetylcysteine
buffer the oxidative stress and improve the therapeutic
outcome of Clindamycin/Ibuprofen combination.
Abstract: A mammal-s body can be seen as a blood vessel with
complex tunnels. When heart pumps blood periodically, blood runs
through blood vessels and rebounds from walls of blood vessels.
Blood pressure signals can be measured with complex but periodic
patterns. When an artery is clamped during a surgical operation, the
spectrum of blood pressure signals will be different from that of
normal situation. In this investigation, intestinal artery clamping
operations were conducted to a pig for simulating the situation of
intestinal blocking during a surgical operation. Similarity theory is a
convenient and easy tool to prove that patterns of blood pressure
signals of intestinal artery blocking and unblocking are surely
different. And, the algorithm of Hilbert Huang Transform can be
applied to extract the character parameters of blood pressure pattern.
In conclusion, the patterns of blood pressure signals of two different
situations, intestinal artery blocking and unblocking, can be
distinguished by these character parameters defined in this paper.
Abstract: Dietary macro and micro nutrients in their respective proportion and fractions present a practical potential tool to fabricate milk constituents since cells of lactating mammary glands obtain about 80 % of milk synthesis nutrients from blood, reflecting the existence of an isotonic equilibrium between blood and milk. Diverting milk biosynthetic activities through manipulation of nutrients towards producing milk not only keeping in view its significance as natural food but also as food item which prevents or dilutes the adverse effects of some diseases (like cardiovascular problem by saturated milk fat intake) has been area of interest in the last decade. Nutritional modification / supplementation has been reported to enhance conjugated linoleic acid, fatty acid type and concentration, essential fatty acid concentration, vitamin B12& C, Se, Cu, I and Fe which are involved to counter the health threats to human well being. Synchronizing dietary nutrients aimed to modify rumen dynamics towards synthesis of nutrients or their precursors to make their drive towards formulated milk constituents presents a practical option. Formulating dietary constituents to design milk constituents will let the farmers, consumers and investors know about the real potential and profit margins associated with this enterprise. This article briefly recapitulates the ways and means to modify milk constituents keeping an eye on human health and well being issues, which allows milk to serve more than a food item.
Abstract: In vitro gastro-duodenal digestion model was used to investigate the changes of emulsions under digestion conditions. Oil in water emulsions stabilized by whey proteins (2%) and stabilized by whey proteins (2%) with addition of carboxymethyl cellulose (0.75%) as gelling agent of continuous phase were prepared at pH7. Both emulsions were destabilized under gastric conditions; however the protective role of carboxymethyl cellulose was indicated by recording delay of fat digestibility of this emulsion. In the presence of carboxymethyl cellulose whey proteins on the interfacial surface of droplets were more resistant to gastric degradation causing limited hydrolysis of fat due to the poor acceptability of lipids for the enzymes. Studies of emulsions using in vivo model supported results from in vitro studies. Lower content of triglycerides in blood serum and higher amount of fecal fat of rats were determined when rats were fed by diet containing emulsion made with whey proteins and carboxymethyl cellulose.
Abstract: The effects of commercial or bovine yeasts on the
performance and blood variables of broiler chickens intoxicated with
aflatoxin were investigated in broilers. Four hundred eighty broilers
(Arbor Acres; 3-wk-old) were randomly assigned to 4 groups. Each
group (120 broiler chickens) was further randomly divided into 6
replicates of 20 chickens. The treatments were control diet without
additives (treatment 1), 250 ppb AFB1 (treatment 2), commercial
yeast, Saccharomyces cerevisiae, (CY 2.5 x 107 CFU/g) + 250 ppb
AFB1 (treatment 3) and bovine yeast, Saccharomyces cerevisiae,
(BY 2.5 x 107 CFU/g + 250 ppb AFB1 (treatment 4). Complete
randomized design (CRD) was used in the experiment. Feed
consumption and body weight were recorded at every five-day
period. On day 42, carcass compositions were determined from 30
birds per treatment. While chicks were sacrificed, 3-4 ml blood
sample was taken and stored frozen at (-20°C) for serum chemical
analysis to determine effects of consumption of diets on blood
chemistry (total protein, albumin, glucose, urea, cholesterol and
triglycerides). There were no significant differences in ADFI among
the treatments(P>0.05). However, BWG, FCR and mortality were
highly significantly different (P
Abstract: In this paper, algorithm estimating the blood pressure
was proposed using the pulse transit time (PTT) as a more convenient
method of measuring the blood pressure. After measuring ECG and
pressure pulse, and photoplethysmography, the PTT was calculated
from the acquired signals. Thereafter, the system to indirectly measure
the systolic pressure and the diastolic pressure was composed using
the statistic method. In comparison between the blood pressure
indirectly measured by proposed algorithm estimating the blood
pressure and real blood pressure measured by conventional
sphygmomanometer, the systolic pressure indicates the mean error of
±3.24mmHg and the standard deviation of 2.53mmHg, while the
diastolic pressure indicates the satisfactory result, that is, the mean
error of ±1.80mmHg and the standard deviation of 1.39mmHg. These
results are satisfied with the regulation of ANSI/AAMI for
certification of sphygmomanometer that real measurement error value
should be within the mean error of ±5mmHg and the standard
deviation of 8mmHg. These results are suggest the possibility of
applying to portable and long time blood pressure monitoring system
hereafter.
Abstract: Photoplethysmography is a simple measurement of the
variation in blood volume in tissue. It detects the pulse signal of heart
beat as well as the low frequency signal of vasoconstriction and
vasodilation. The transmission type measurement is limited to only a
few specific positions for example the index finger that have a short
path length for light. The reflectance type measurement can be
conveniently applied on most parts of the body surface. This study
analyzed the factors that determine the quality of reflectance
photoplethysmograph signal including the emitter-detector distance,
wavelength, light intensity, and optical properties of skin tissue.
Light emitting diodes (LEDs) with four different visible
wavelengths were used as the light emitters. A phototransistor was
used as the light detector. A micro translation stage adjusts the
emitter-detector distance from 2 mm to 15 mm.
The reflective photoplethysmograph signals were measured on
different sites. The optimal emitter-detector distance was chosen to
have a large dynamic range for low frequency drifting without signal
saturation and a high perfusion index. Among these four wavelengths,
a yellowish green (571nm) light with a proper emitter-detection
distance of 2mm is the most suitable for obtaining a steady and reliable
reflectance photoplethysmograph signal
Abstract: This paper presents a method for the detection of OD in the retina which takes advantage of the powerful preprocessing techniques such as the contrast enhancement, Gabor wavelet transform for vessel segmentation, mathematical morphology and Earth Mover-s distance (EMD) as the matching process. The OD detection algorithm is based on matching the expected directional pattern of the retinal blood vessels. Vessel segmentation method produces segmentations by classifying each image pixel as vessel or nonvessel, based on the pixel-s feature vector. Feature vectors are composed of the pixel-s intensity and 2D Gabor wavelet transform responses taken at multiple scales. A simple matched filter is proposed to roughly match the direction of the vessels at the OD vicinity using the EMD. The minimum distance provides an estimate of the OD center coordinates. The method-s performance is evaluated on publicly available DRIVE and STARE databases. On the DRIVE database the OD center was detected correctly in all of the 40 images (100%) and on the STARE database the OD was detected correctly in 76 out of the 81 images, even in rather difficult pathological situations.
Abstract: Hypertension is characterized with stress on the heart and blood vessels thus increasing the risk of heart attack and renal diseases. The Renin angiotensin system (RAS) plays a major role in blood pressure control. Renin is the enzyme that controls the RAS at the rate-limiting step. Our aim is to develop new drug-like leads which can inhibit renin and thereby emerge as therapeutics for hypertension. To achieve this, molecular dynamics (MD) simulation and receptor-based pharmacophore modeling were implemented, and three rennin-inhibitor complex structures were selected based on IC50 value and scaffolds of inhibitors. Three pharmacophore models were generated considering conformations induced by inhibitor. The compounds mapped to these models were selected and subjected to drug-like screening. The identified hits were docked into the active site of renin. Finally, hit1 satisfying the binding mode and interaction energy was selected as possible lead candidate to be used in novel renin inhibitors.
Abstract: A analysis on the conventional the blood pressure estimation method using an oscillometric sphygmomanometer was
performed through a computer simulation using an arterial pressure-volume (APV) model. Traditionally, the maximum amplitude algorithm (MAP) was applied on the oscillation waveforms of the APV model to obtain the mean arterial pressure and the characteristic ratio. The estimation of mean arterial pressure and
characteristic ratio was significantly affected with the shape of the blood pressure waveforms and the cutoff frequency of high-pass filter
(HPL) circuitry. Experimental errors are due to these effects when estimating blood pressure. To find out an algorithm independent from
the influence of waveform shapes and parameters of HPL, the volume
oscillation of the APV model and the phase shift of the oscillation with fast fourier transform (FFT) were testified while increasing the cuff
pressure from 1 mmHg to 200 mmHg (1 mmHg per second). The phase shift between the ranges of volume oscillation was then only observed between the systolic and the diastolic blood pressures. The same results were also obtained from the simulations performed on two different the arterial blood pressure waveforms and one
hyperthermia waveform.
Abstract: New graph similarity methods have been proposed in this work with the aim to refining the chemical information extracted from molecules matching. For this purpose, data fusion of the isomorphic and nonisomorphic subgraphs into a new similarity measure, the Approximate Similarity, was carried out by several approaches. The application of the proposed method to the development of quantitative structure-activity relationships (QSAR) has provided reliable tools for predicting several pharmacological parameters: binding of steroids to the globulin-corticosteroid receptor, the activity of benzodiazepine receptor compounds, and the blood brain barrier permeability. Acceptable results were obtained for the models presented here.
Abstract: Kwashiorkor is one of nutritional problem in
Indonesia, which lead to decrease immune system. This condition
causes susceptibility to infectious disease, especially tuberculosis.
Development of new tuberculosis vaccine will be an important
strategy to eliminate tuberculosis in kwashiorkor. Previous research
showed that 38-kDa Mycobacterium tuberculosis protein is one of the
potent immunogen. However, the role of oral immunization with 38-
kDa Mycobacterium tuberculosis protein to the number of
lymphocytes in the rat model of kwashiorkor is still unknown. We
used kwashiorkor rat model groups with 4% and 2% low protein diet.
Oral immunization with 38-kDa Mycobacterium tuberculosis protein
given with 2 booster every week. The lymphocytes number were
measured by flowcytometry. There was no significant difference
between the number of lymphocytes in the normal rat group and the
kwashiorkor rat groups. It may reveal the role of 38-kDa
Mycobacterium tuberculosis protein as a potent immunogen that can
increase the lymphocytes number from kwashiorkor rat model same
as normal rat.
Abstract: In order to be able to automatically differentiate
between two modes of permanent flow of a liquid simulating blood,
it was imperative to put together a data bank. Thus, the acquisition of
the various amplitude spectra of the Doppler signal of this liquid in
laminar flow and other spectra in turbulent flow enabled us to
establish an automatic difference between the two modes. According
to the number of parameters and their nature, a comparative study
allowed us to choose the best classifier.
Abstract: An attempt has been made to develop a
seminumerical model to study temperature variations in dermal
layers of human limbs. The model has been developed for two
dimensional steady state case. The human limb has been assumed to
have elliptical cross section. The dermal region has been divided
into three natural layers namely epidermis, dermis and subdermal
tissues. The model incorporates the effect of important physiological
parameters like blood mass flow rate, metabolic heat generation, and
thermal conductivity of the tissues. The outer surface of the limb is
exposed to the environment and it is assumed that heat loss takes
place at the outer surface by conduction, convection, radiation, and
evaporation. The temperature of inner core of the limb also varies at
the lower atmospheric temperature. Appropriate boundary conditions
have been framed based on the physical conditions of the problem.
Cubic splines approach has been employed along radial direction and
Fourier series along angular direction to obtain the solution. The
numerical results have been computed for different values of
eccentricity resembling with the elliptic cross section of the human
limbs. The numerical results have been used to obtain the
temperature profile and to study the relationships among the various
physiological parameters.
Abstract: Pattern recognition is the research area of Artificial
Intelligence that studies the operation and design of systems that
recognize patterns in the data. Important application areas are image
analysis, character recognition, fingerprint classification, speech
analysis, DNA sequence identification, man and machine
diagnostics, person identification and industrial inspection. The
interest in improving the classification systems of data analysis is
independent from the context of applications. In fact, in many
studies it is often the case to have to recognize and to distinguish
groups of various objects, which requires the need for valid
instruments capable to perform this task. The objective of this article
is to show several methodologies of Artificial Intelligence for data
classification applied to biomedical patterns. In particular, this work
deals with the realization of a Computer-Aided Detection system
(CADe) that is able to assist the radiologist in identifying types of
mammary tumor lesions. As an additional biomedical application of
the classification systems, we present a study conducted on blood
samples which shows how these methods may help to distinguish
between carriers of Thalassemia (or Mediterranean Anaemia) and
healthy subjects.
Abstract: Recently, the health of retired National Football
League players, particularly lineman has been investigated. A
number of studies have reported increased cardiometabolic risk,
premature cardiovascular disease and incidence of type 2 diabetes.
Rugby union players have somatotypes very similar to National
Football League players which suggests that rugby players may have
similar health risks. The International Golden Oldies World Rugby
Festival (GORF) provided a unique opportunity to investigate the
demographics of veteran rugby players. METHODOLOGIES: A
cross-sectional, observational study was completed using an online
web-based questionnaire that consisted of medical history and
physiological measures. Data analysis was completed using a one
sample t-test (50yrs) and Chi-square test. RESULTS:
A total of 216 veteran rugby competitors (response rate = 6.8%)
representing 10 countries, aged 35-72 yrs (mean 51.2, S.D. ±8.0),
participated in the online survey. As a group, the incidence of current
smokers was low at 8.8% (avg 72.4 cigs/wk) whilst the percentage
consuming alcohol was high (93.1% (avg 11.2 drinks/wk).
Competitors reported the following top six chronic
diseases/disorders; hypertension (18.6%), arthritis (OA/RA, 11.5%),
asthma (9.3%), hyperlipidemia (8.2%), diabetes (all types, 7.5%) and
gout (6%), there were significant differences between groups with
regard to cancer (all types) and migraines. When compared to the
Australian general population (Australian Bureau of Statistics data,
n=18,000), GORF competitors had a significantly lower incidence of
anxiety (p