Scholarly

Identification of Binding Proteins That Interact with BVDV E2 Protein in Bovine Trophoblast Cell

Year: 2013 Volume: 7 Issue: 3 202 - 205 Pages

Abstract: Bovine viral diarrhea virus (BVDV) can cause lifelong persistent infection. One reason for the phenomena is attributed to BVDV infection to placenta tissue. However the mechanisms that BVDV invades into placenta tissue remain unclear. To clarify the molecular mechanisms, we investigated the possible means that BVDV entered into bovine trophoblast cells (TPC). Yeast two-hybrid system was used to identify proteins extracted from TPC, which interact with BVDV envelope glycoprotein E2. A PGbkt7-E2 yeast expression vector and TPC cDNA library were constructed. Through two rounds of screening, three positive clones were identified. Sequencing analysis indicated that all the three positive clones encoded the same protein clathrin. Physical interaction between clathrin and BVDV E2 protein was further confirmed by coimmunoprecipitation experiments. This result suggested that the clathrin might play a critical role in the process of BVDV entry into placenta tissue and might be a novel antiviral target for preventing BVDV infection.

FEM Simulation of HE Blast-Fragmentation Warhead and the Calculation of Lethal Range

Year: 2012 Volume: 6 Issue: 6 1077 - 1081 Pages

Abstract: This paper presents the simulation of fragmentation warhead using a hydrocode, Autodyn. The goal of this research is to determine the lethal range of such a warhead. This study investigates the lethal range of warheads with and without steel balls as preformed fragments. The results from the FE simulation, i.e. initial velocities and ejected spray angles of fragments, are further processed using an analytical approach so as to determine a fragment hit density and probability of kill of a modelled warhead. In order to simulate a plenty of preformed fragments inside a warhead, the model requires expensive computation resources. Therefore, this study attempts to model the problem in an alternative approach by considering an equivalent mass of preformed fragments to the mass of warhead casing. This approach yields approximately 7% and 20% difference of fragment velocities from the analytical results for one and two layers of preformed fragments, respectively. The lethal ranges of the simulated warheads are 42.6 m and 56.5 m for warheads with one and two layers of preformed fragments, respectively, compared to 13.85 m for a warhead without preformed fragment. These lethal ranges are based on the requirement of fragment hit density. The lethal ranges which are based on the probability of kill are 27.5 m, 61 m and 70 m for warheads with no preformed fragment, one and two layers of preformed fragments, respectively.

Potential Effects of Human Bone Marrow Non- Mesenchymal Mononuclear Cells on Neuronal Differentiation

Year: 2011 Volume: 5 Issue: 12 664 - 668 Pages

Abstract: Bone marrow-derived stem cells have been widely studied as an alternative source of stem cells. Mesenchymal stem cells (MSCs) were mostly investigated and studies showed MSCs can promote neurogenesis. Little is known about the non-mesenchymal mononuclear cell fraction, which contains both hematopoietic and nonhematopoietic cells, including monocytes and endothelial progenitor cells. This study focused on unfractionated bone marrow mononuclear cells (BMMCs), which remained 72 h after MSCs were adhered to the culture plates. We showed that BMMC-conditioned medium promoted morphological changes of human SH-SY5Y neuroblastoma cells from an epithelial-like phenotype towards a neuron-like phenotype as indicated by an increase in neurite outgrowth, like those observed in retinoic acid (RA)-treated cells. The result could be explained by the effects of trophic factors released from BMMCs, as shown in the RT-PCR results that BMMCs expressed nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and ciliary neurotrophic factor (CNTF). Similar results on the cell proliferation rate were also observed between RA-treated cells and cells cultured in BMMC-conditioned medium, suggesting that cells creased proliferating and differentiated into a neuronal phenotype. Using real-time RT-PCR, a significantly increased expression of tyrosine hydroxylase (TH) mRNA in SHSY5Y cells indicated that BMMC-conditioned medium induced catecholaminergic identities in differentiated SH-SY5Y cells.

The Effects of Shot and Grit Blasting Process Parameters on Steel Pipes Coating Adhesion

Year: 2010 Volume: 4 Issue: 6 493 - 501 Pages

Authors:
Saeed Khorasanizadeh

Abstract: Adhesion strength of exterior or interior coating of steel pipes is too important. Increasing of coating adhesion on surfaces can increase the life time of coating, safety factor of transmitting line pipe and decreasing the rate of corrosion and costs. Preparation of steel pipe surfaces before doing the coating process is done by shot and grit blasting. This is a mechanical way to do it. Some effective parameters on that process, are particle size of abrasives, distance to surface, rate of abrasive flow, abrasive physical properties, shapes, selection of abrasive, kind of machine and its power, standard of surface cleanness degree, roughness, time of blasting and weather humidity. This search intended to find some better conditions which improve the surface preparation, adhesion strength and corrosion resistance of coating. So, this paper has studied the effect of varying abrasive flow rate, changing the abrasive particle size, time of surface blasting on steel surface roughness and over blasting on it by using the centrifugal blasting machine. After preparation of numbers of steel samples (according to API 5L X52) and applying epoxy powder coating on them, to compare strength adhesion of coating by Pull-Off test. The results have shown that, increasing the abrasive particles size and flow rate, can increase the steel surface roughness and coating adhesion strength but increasing the blasting time can do surface over blasting and increasing surface temperature and hardness too, change, decreasing steel surface roughness and coating adhesion strength.

Effect of White Kwao Extract (Pueraria mirifica) on in vitro Development and Implantation Rate of Mouse Embryo

Year: 2013 Volume: 7 Issue: 1 50 - 53 Pages

Authors:
Sansani Rungrattawatchai

Abstract: The White Kwao (Pueraria mirifica), a potent phytoestrogenic medicinal plant, has long been use in Thailand as a traditional folkmedicine. However, no scientific information of the direct effect of White Kwao on the development of mammalian embryo was available. Therefore, the purpose of this study was to investigate the effect of White Kwao extract on the in vitro development and implantation rate of mouse embryos. This study was designed into two experiments. In the first experiment, the two-cell stage mouse embryos were collected from the oviduct of superovulated mature female mice, and randomly cultured in three different media, the M16, M16 supplemented with 0.52μg esthinylestradiol-17β, and M16 supplemented with 10 mg/ml White Kwao extract. The culture was incubated in CO2 incubator at 37 oC . After the embryos were cultivated, the developments of embryos were observed every 24 hours for 5 days. The development rate of embryos from the two-cell stage to blastocyst stage in the media was with White Kwao was significantly higher (p

Expression of Leucaena Leucocephala de Wit Chitinase in Transgenic Koshihikari Rice

Year: 2009 Volume: 3 Issue: 11 546 - 551 Pages

Abstract: The cDNA encoding the 326 amino acids of a Class I basic chitinase gene from Leucaena leucocephala de Wit (KB3, Genbank accession: AAM49597) was cloned under the control of CaMV35S promoter in pCAMBIA 1300 and transferred to Koshihikari. Calli of Koshihikari rice was transformed with agrobacterium with this construct expressing the chitinase and β- glucouronidase (GUS). The frequencies of calli 90 % has been obtained from rice seedlings cultured on NB medium. The high regeneration frequencies, 74% was obtained from calli cultured on regeneration medium containing 4 mg/l BAP, and 7 g/l phytagel at 25°C. Various factors were studied in order to establish a procedure for the transformation of Koshihikari Agrobacterium tumefaciens. Supplementation of 50 mM acetosyringone to the medium during coculivation was important to enhance the frequency to transient transformation. The 4 week-old scutellum-derived calli were excellent starting materials. Selection medium based on NB medium supplement with 40 mg/l hygromycin and 400 mg/l cefotaxime were an optimized medium for selection of transformed rice calli. The percentage of transformation 70 was obtained. Recombinant calli and regenerated rice plants were checked the expression of chitinase and gus by PCR, northern blot gel, southern blot gel, and gus assay. Chitinase and gus were expressed in all parts of recombinant rice. The rice line expressing the KB3 chiitnase was more resistant to the blast fungus Fusarium monoliforme than control line.

Tuberculin, Tetanus Immunoglobulin and DPT Vaccine as an Avian in vivo T- Lymphocyte Mitogens

Year: 2013 Volume: 7 Issue: 7 529 - 531 Pages

Authors:
Ibrahim Mohammed Saeed Shnawa

Abstract: The avian phytohaemagglutinin skin test is being proved as an in vivo system for the evaluation an avian in vivo T cell mitogenicity. The test system was one week old Gallus domesticus broiler Chickens. Five replicates were done for each of the whole, 1:10 dilutions of each of 0.05 IU tuberculin, tetanus immunoglobulin and DPT vaccine as test materials. The evaluation parameters were the skin indurations and lymphoblast percentages in bone marrow lymphocytes. Tuberculin indurations were 2.06 and 1.26mm for 0.05 IU respectively while lymphoblast percent were 0.234 and 0.1 accordingly. The skin indurations of 135mg/ml and 1.35mg/ml tetanus immunoglobulin were 4.86 and 3.96mm while lymphoblast percentages were 0.3 and 0.14 respectively. The whole DPT and 1:10 concentration were with 4.5 and 3.2mm while their lymphoblast percentages were 0.28 and 0.12 accordingly. Thus the mitogenicity of the test materials was of dependant type.

Analytical Proposal to Damage Assessment of Buried Continuous Pipelines during External Blast Loading

Year: 2011 Volume: 5 Issue: 11 558 - 562 Pages

Abstract: In this paper, transversal vibration of buried pipelines during loading induced by underground explosions is analyzed. The pipeline is modeled as an infinite beam on an elastic foundation, so that soil-structure interaction is considered by means of transverse linear springs along the pipeline. The pipeline behavior is assumed to be ideal elasto-plastic which an ultimate strain value limits the plastic behavior. The blast loading is considered as a point load, considering the affected length at some point of the pipeline, in which the magnitude decreases exponentially with time. A closed-form solution for the quasi-static problem is carried out for both elastic and elasticperfect plastic behaviors of pipe materials. At the end, a comparative study on steel and polyethylene pipes with different sizes buried in various soil conditions, affected by a predefined underground explosion is conducted, in which effect of each parameter is discussed.

Numerical Simulation of Progressive Collapse for a Reinforced Concrete Building

Year: 2013 Volume: 7 Issue: 4 290 - 293 Pages

Abstract: Though nonlinear dynamic analysis using a specialized hydro-code such as AUTODYN is accurate and useful tool for progressive collapse assessment of a multi-story building subjected to blast load, it takes too much time to be applied to a practical simulation of progressive collapse of a tall building. In this paper, blast analysis of a RC frame structure using a simplified model with Reinforcement Contact technique provided in Ansys Workbench was introduced and investigated on its accuracy. Even though the simplified model has a fraction of elements of the detailed model, the simplified model with this modeling technique shows similar structural behavior under the blast load to the detailed model. The proposed modeling method can be effectively applied to blast loading progressive collapse analysis of a RC frame structure.

Uniformity of Dose Distribution in Radiation Fields Surrounding the Spine using Film Dosimetry and Comparison with 3D Treatment Planning Software

Year: 2011 Volume: 5 Issue: 11 566 - 568 Pages

Abstract: The overall penumbra is usually defined as the distance, p20–80, separating the 20% and 80% of the dose on the beam axis at the depth of interest. This overall penumbra accounts also for the fact that some photons emitted by the distal parts of the source are only partially attenuated by the collimator. Medulloblastoma is the most common type of childhood brain tumor and often spreads to the spine. Current guidelines call for surgery to remove as much of the tumor as possible, followed by radiation of the brain and spinal cord, and finally treatment with chemotherapy. The purpose of this paper was to present results on an Uniformity of dose distribution in radiation fields surrounding the spine using film dosimetry and comparison with 3D treatment planning software.

Hydrodynamic Characteristics of Dry Beneficiation of Iron Ore and Coal in a Fast Fluidized Bed

Year: 2010 Volume: 4 Issue: 5 305 - 308 Pages

Abstract: Iron ore and coal are the two major important raw materials being used in Iron making industries. Usually ore fines containing around 5% Alumina are rejected due to higher proportion of alumina. Therefore, a technology or process which may reduce the alumina content by 2% by beneficiation process will be highly attractive . In addition fine coals with ash content is used nearly 12% is directly injected in blast furnace. Fast fluidization is a technology by using dry beneficiation of coal and iron ore can be done. During the fluidization process the iron ore band coal is fluidized at high velocity in the riser of a fast fluidized bed, the heavier and coarse particles is generally settled at the bottom in a dense zone of the riser while the finer and lighter particle are entrained to the top dilute zone and then via a cyclone is fed back to the bottom of the riser column. Most of the alumina and low ash fine size coals being lighter are expected to move up to the riser and by a natural beneficiation of ores is expected to take place in the riser. Therefore in this study an attempt has been made for dry beneficiation of iron ore and coal in a fluidized bed and its hydrodynamic characterization.

Effect of Cold Plasma-Surface Modification on Surface Wettability and Initial Cell Attachment

Year: 2009 Volume: 3 Issue: 10 497 - 501 Pages

Abstract: A thin coating of hexamethyldisiloxane and subsequent O2-plasma treatment was performed on mirror-polished titanium in order to regulate the wide range of wettability including 106 and almost 0 degrees of contact angles. The adsorption behavior of fibronectin and albumin in both individual and competitive mode, and initial attachment of fibroblasts and osteoblasts were investigated. Individually, fibronectin adsorption showed a biphasic inclination, whereas albumin showed greater adsorption to hydrophobic surfaces. In competitive mode, in solution containing both fibronectin and albumin, fibronectin showed greater adsorption on hydrophilic surfaces, whereas Alb predominantly adsorbed on hydrophobic surfaces. Initial attachment of both cells increased with increase in surface wettability, in particular, on super-hydrophilic surface, which correlated well with fibronectin adsorption in competitive mode. These results suggest that a cold plasma-surface modification enabled to regulate the surface wettability, and fibronectin adsorption may be responsible for increasing cell adhesion on hydrophilic surfaces in a body fluid

An Identification Method of Geological Boundary Using Elastic Waves

Year: 2010 Volume: 4 Issue: 11 356 - 362 Pages

Abstract: This paper focuses on a technique for identifying the geological boundary of the ground strata in front of a tunnel excavation site using the first order adjoint method based on the optimal control theory. The geological boundary is defined as the boundary which is different layers of elastic modulus. At tunnel excavations, it is important to presume the ground situation ahead of the cutting face beforehand. Excavating into weak strata or fault fracture zones may cause extension of the construction work and human suffering. A theory for determining the geological boundary of the ground in a numerical manner is investigated, employing excavating blasts and its vibration waves as the observation references. According to the optimal control theory, the performance function described by the square sum of the residuals between computed and observed velocities is minimized. The boundary layer is determined by minimizing the performance function. The elastic analysis governed by the Navier equation is carried out, assuming the ground as an elastic body with linear viscous damping. To identify the boundary, the gradient of the performance function with respect to the geological boundary can be calculated using the adjoint equation. The weighed gradient method is effectively applied to the minimization algorithm. To solve the governing and adjoint equations, the Galerkin finite element method and the average acceleration method are employed for the spatial and temporal discretizations, respectively. Based on the method presented in this paper, the different boundary of three strata can be identified. For the numerical studies, the Suemune tunnel excavation site is employed. At first, the blasting force is identified in order to perform the accuracy improvement of analysis. We identify the geological boundary after the estimation of blasting force. With this identification procedure, the numerical analysis results which almost correspond with the observation data were provided.

Induction of Apoptosis by Newcastle Disease Virus Strains AF220 and V4-UPM in Human Promyelocytic Leukemia (HL60) and Human T-Lymphoblastic Leukemia (CEM-SS) Cells

Year: 2012 Volume: 6 Issue: 4 144 - 148 Pages

Abstract: Newcastle Disease Virus (NDV), an avian paramyxovirus, is a highly contagious, generalised virus disease of domestic poultry and wild birds characterized by gastro-intestinal, respiratory and nervous signs. In this study, it was shown that NDV strain AF2240 and V4-UPM are cytolytic to Human Promyelocytic Leukemia, HL60 and Human T-lymphoblastic Leukemia, CEM-SS cells. Results from MTT cytolytic assay showed that CD50 for NDV AF2240 against HL60 was 130 HAU and NDV V4-UPM against HL60 and CEM-SS were 110.6 and 150.9 HAU respectively. Besides, both strains were found to inhibit the proliferation of cells in a dose dependent manner. The mode of cell death either by apoptosis or necrosis was further analyzed using acridine orange and propidium iodide (AO/PI) staining. Our results showed that both NDV strains induced primarily apoptosis in treated cells at CD50 concentration. In conclusion, both NDV strains caused cytolytic effects primarily via apoptosis in leukemia cells.

Data Mining for Cancer Management in Egypt Case Study: Childhood Acute Lymphoblastic Leukemia

Year: 2007 Volume: 1 Issue: 8 481 - 486 Pages

Abstract: Data Mining aims at discovering knowledge out of data and presenting it in a form that is easily comprehensible to humans. One of the useful applications in Egypt is the Cancer management, especially the management of Acute Lymphoblastic Leukemia or ALL, which is the most common type of cancer in children. This paper discusses the process of designing a prototype that can help in the management of childhood ALL, which has a great significance in the health care field. Besides, it has a social impact on decreasing the rate of infection in children in Egypt. It also provides valubale information about the distribution and segmentation of ALL in Egypt, which may be linked to the possible risk factors. Undirected Knowledge Discovery is used since, in the case of this research project, there is no target field as the data provided is mainly subjective. This is done in order to quantify the subjective variables. Therefore, the computer will be asked to identify significant patterns in the provided medical data about ALL. This may be achieved through collecting the data necessary for the system, determimng the data mining technique to be used for the system, and choosing the most suitable implementation tool for the domain. The research makes use of a data mining tool, Clementine, so as to apply Decision Trees technique. We feed it with data extracted from real-life cases taken from specialized Cancer Institutes. Relevant medical cases details such as patient medical history and diagnosis are analyzed, classified, and clustered in order to improve the disease management.

Virulent-GO: Prediction of Virulent Proteins in Bacterial Pathogens Utilizing Gene Ontology Terms

Year: 2009 Volume: 3 Issue: 5 242 - 249 Pages

Abstract: Prediction of bacterial virulent protein sequences can give assistance to identification and characterization of novel virulence-associated factors and discover drug/vaccine targets against proteins indispensable to pathogenicity. Gene Ontology (GO) annotation which describes functions of genes and gene products as a controlled vocabulary of terms has been shown effectively for a variety of tasks such as gene expression study, GO annotation prediction, protein subcellular localization, etc. In this study, we propose a sequence-based method Virulent-GO by mining informative GO terms as features for predicting bacterial virulent proteins. Each protein in the datasets used by the existing method VirulentPred is annotated by using BLAST to obtain its homologies with known accession numbers for retrieving GO terms. After investigating various popular classifiers using the same five-fold cross-validation scheme, Virulent-GO using the single kind of GO term features with an accuracy of 82.5% is slightly better than VirulentPred with 81.8% using five kinds of sequence-based features. For the evaluation of independent test, Virulent-GO also yields better results (82.0%) than VirulentPred (80.7%). When evaluating single kind of feature with SVM, the GO term feature performs much well, compared with each of the five kinds of features.

Reliability Analysis of P-I Diagram Formula for RC Column Subjected to Blast Load

Year: 2013 Volume: 7 Issue: 8 584 - 588 Pages

Abstract: This study was conducted published to investigate there liability of the equation pressure-impulse (PI) reinforced concrete column inprevious studies. Equation involves three different levels of damage criteria known as D =0. 2, D =0. 5 and D =0. 8.The damage criteria known as a minor when 0-0.2, 0.2-0.5is known as moderate damage, high damage known as 0.5-0.8, and 0.8-1 of the structure is considered a failure. In this study, two types of reliability analyzes conducted. First, using pressure-impulse equation with different parameters. The parameters involved are the concrete strength, depth, width, and height column, the ratio of longitudinal reinforcement and transverse reinforcement ratio. In the first analysis of the reliability of this new equation is derived to improve the previous equations. The second reliability analysis involves three types of columns used to derive the PI curve diagram using the derived equation to compare with the equation derived from other researchers and graph minimum standoff versus weapon yield Federal Emergency Management Agency (FEMA). The results showed that the derived equation is more accurate with FEMA standards than previous researchers.

Derivation of Empirical Formulae to Predict Pressure and Impulsive Asymptotes for P-I Diagrams of One-way RC Panels

Year: 2013 Volume: 7 Issue: 8 580 - 583 Pages

Abstract: There are only limited studies that directly correlate the increase in reinforced concrete (RC) panel structural capacities in resisting the blast loads with different RC panel structural properties in terms of blast loading characteristics, RC panel dimensions, steel reinforcement ratio and concrete material strength. In this paper, numerical analyses of dynamic response and damage of the one-way RC panel to blast loads are carried out using the commercial software LS-DYNA. A series of simulations are performed to predict the blast response and damage of columns with different level and magnitude of blast loads. The numerical results are used to develop pressureimpulse (P-I) diagrams of one-way RC panels. Based on the numerical results, the empirical formulae are derived to calculate the pressure and impulse asymptotes of the P-I diagrams of RC panels. The results presented in this paper can be used to construct P-I diagrams of RC panels with different concrete and reinforcement properties. The P-I diagrams are very useful to assess panel capacities in resisting different blast loads.

Artificial Neural Network Prediction for Coke Strength after Reaction and Data Analysis

Year: 2010 Volume: 4 Issue: 9 554 - 558 Pages

Abstract: In this paper, the requirement for Coke quality prediction, its role in Blast furnaces, and the model output is explained. By applying method of Artificial Neural Networking (ANN) using back propagation (BP) algorithm, prediction model has been developed to predict CSR. Important blast furnace functions such as permeability, heat exchanging, melting, and reducing capacity are mostly connected to coke quality. Coke quality is further dependent upon coal characterization and coke making process parameters. The ANN model developed is a useful tool for process experts to adjust the control parameters in case of coke quality deviations. The model also makes it possible to predict CSR for new coal blends which are yet to be used in Coke Plant. Input data to the model was structured into 3 modules, for tenure of past 2 years and the incremental models thus developed assists in identifying the group causing the deviation of CSR.

Computational Identification of MicroRNAs and their Targets in two Species of Evergreen Spruce Tree (Picea)

Year: 2011 Volume: 5 Issue: 3 128 - 133 Pages

Abstract: MicroRNAs (miRNAs) are small, non-coding and regulatory RNAs about 20 to 24 nucleotides long. Their conserved nature among the various organisms makes them a good source of new miRNAs discovery by comparative genomics approach. The study resulted in 21 miRNAs of 20 pre-miRNAs belonging to 16 families (miR156, 157, 158, 164, 165, 168, 169, 172, 319, 390, 393, 394, 395, 400, 472 and 861) in evergreen spruce tree (Picea). The miRNA families; miR 157, 158, 164, 165, 168, 169, 319, 390, 393, 394, 400, 472 and 861 are reported for the first time in the Picea. All 20 miRNA precursors form stable minimum free energy stem-loop structure as their orthologues form in Arabidopsis and the mature miRNA reside in the stem portion of the stem loop structure. Sixteen (16) miRNAs are from Picea glauca and five (5) belong to Picea sitchensis. Their targets consist of transcription factors, growth related, stressed related and hypothetical proteins.

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