Induction of Apoptosis by Newcastle Disease Virus Strains AF220 and V4-UPM in Human Promyelocytic Leukemia (HL60) and Human T-Lymphoblastic Leukemia (CEM-SS) Cells
Newcastle Disease Virus (NDV), an avian
paramyxovirus, is a highly contagious, generalised virus disease of
domestic poultry and wild birds characterized by gastro-intestinal,
respiratory and nervous signs. In this study, it was shown that NDV
strain AF2240 and V4-UPM are cytolytic to Human Promyelocytic
Leukemia, HL60 and Human T-lymphoblastic Leukemia, CEM-SS
cells. Results from MTT cytolytic assay showed that CD50 for NDV
AF2240 against HL60 was 130 HAU and NDV V4-UPM against
HL60 and CEM-SS were 110.6 and 150.9 HAU respectively.
Besides, both strains were found to inhibit the proliferation of cells in
a dose dependent manner. The mode of cell death either by apoptosis
or necrosis was further analyzed using acridine orange and propidium
iodide (AO/PI) staining. Our results showed that both NDV strains
induced primarily apoptosis in treated cells at CD50 concentration. In
conclusion, both NDV strains caused cytolytic effects primarily via
apoptosis in leukemia cells.
[1] K. W. Reichard, R. M. Lorence, C. J. Cascino, M. E. Peeples, R. J.
Walter, M. B. Fernando, H. M. Reyes and J. A. Greager. "Newcastle
disease virus selectively kills human tumor cells," J. Surg. Res., 1992,
52: 448-453.
[2] A. R. Omar, A. Ideris, A. M. Ali, F. Othman, K. Yusoff, J. M.
Abdullah et al. "An overview of the development of Newcastle disease
virus as an anti-cancer therapy," Malaysian J Med Sci., 2002, 9: 4-12.
[3] D. J. Alexander, "Newcastle disease and other avian Paramyxoviridae
infections," in Diseases of Poultry, 10th ed., B. W. Calneck, Ed. Ames,
IA: Iowa State University Press, 1997, pp. 541-569.
[4] M. A. Mayo. 2002. "A summary of taxonomic changes recently
approved by ICTV". Arch. Virol., 2002, 147: 1655-1666.
[5] D. J. Alexander, "Newcastle disease," in A Laboratory Manual for the
Isolation and Identification of Avian Pathogens, 3rd ed., H. G.
Purchase, L. H. Arp, C. H. Domermuth and J. E.Pearson, Eds. Kennett
Square, PA: American Association of Avian Pathologists, 1989, pp.
114-120.
[6] F. M. Batliwalla, B. A. Bateman, D. Serrano, D. Murray, S. Macphail,
V. C. Maino et al. "15-year follow-up of AJCC stage III malignant
melanoma patients treated postsurgically with Newcastle disease virus
(NDV) oncolysate and determination of alterations in the CD8 T cell
Noun 1. CD8 T cell - T cell with CD8 receptor that recognizes antigens
on the surface of a virus-infected cell and binds to the infected cell and
kill itCD8 cell, cytotoxic T cell, killer cell, killer T cell repertoire,"
Mol Med., 1998, 4: 783-794.
[7] V. Schirrmacher, A. Griesbach and T. Ahlert. "Anti-tumour effects of
Newcastle Disease Virus in vivo: local versus systemic effects,"
Int J Oncol., 2001, 18 : 945-52.
[8] R. M. Lorence, A. L. Pecora, P. P. Major, S. J. Hotte, S. A. Laurie, M.
S. Roberts et al. 2003. "Overview of phase I studies of intravenous
administration of PV701, an oncolytic virus," Curr Opin Mol Ther.,
2003, 5: 618-624.
[9] P. B. Spradbrow and J. L. Samue, "Oral Newcastle disease vaccine in
experimental chickens in Australia," in Newcastle Disease in poultry,
J. W. Copland, Ed. ACIAR: Canberra, 1987, pp: 44-49. 1987.
[10] H. Westbury. "Newcastle disease virus: an evolving pathogen," Avian
Pathology, 2001, 30: 5-11.
[11] M. C. Lai and A. L. Ibrahim, "Velogenic-viscerotropic Newcastle
disease virus," in New castle disease virus in poultry, J.W. Copland Ed.
ACIAR Monograph.1987.
[12] W. S. Tan, C. H. Lau, B. K. Ng, A. L. Ibrahim and K. Yusoff.
"Nucleotide sequence of the haemagglutinin-neuraminidase (HN) gene
of a Malaysian heat resistant viscerotropic-velogenic Newcastle disease
virus," DNA Sequence, 1995, 6: 47-50.
[13] K. Yusoff and W. S. Tan. "Newcastle disease virus: macromolecules
and opportunities," Avian Pathology, 2001, 30: 439-455.
[14] M. Meyyappan. "Oncolytic effect of Newcastle disease virus on the
MCF-7 and MDA-231 breast cancer cell lines," Master Thesis,
Universiti Putra Malaysia, Malaysia, 2003.
[15] H. S. M. Wali. "The effects of Newcastle Disease Virus (NDV) on
breast cancer cell lines," Master Thesis, Universiti Putra Malaysia,
Malaysia, 2003.
[16] M. Aied. "Antileukaemic effect of local Newcastle disease virus strains
AF2240 and V4-UPM on leukaemia cell lines in vitro and in vivo," Ph.
D Thesis. Universiti Putra Malaysia, Malaysia, 2007.
[17] Rola Ali, Aied M. Alabsi, Abdul Manaf Ali, Aini Ideris , Abdul
Rahman Omar and Khatijah Yousoff. "Apoptosis Induction and
Cytolytic Effects of Newcastle Disease Virus Strain Af2240 on
DBTRG.05 mg Brain Tumor Cell Line." International Journal of
Cancer Research, 2011, 7: 25-35.
[18] D. J. Alexander, "Newcastle Disease Diagnosis," in Newcastle
Disease, D. J. Alexander, Ed. Kluwer Academic Publishers: Norwell,
Massachusetts, 1988, pp: 147-160.
[19] P. Chambers and A. C. R. Samson. "A new structural protein for
newcastle disease virus," J. General Virol.1980, 50: 155-166.
[20] K. Yusoff, W. S. Tan, C. H. Lau, B. K. Ng and A. L. Ibrahim.
"Sequence of the haemagglutinin-neuraminadase gene of the
Newcastle disease virus oral vaccine strain V4 (UPM)," Avian Pathol.,
1996, 25: 837-844.
[21] R. M. Lorence, P. A. Rood and K. W. Kelley. "Newcastle disease virus
as an antineoplastic agent: induction of tumor necrosis factor-alpha and
augmentation of its cytotoxicity," J Natl Cancer Inst., 1988, 80: 1305-
1312.
[22] U. Zorn, I. Dallmann, J. Grosse J. et al. "Induction of cytokines and
cytotoxicity against tumor cells by Newcastle disease virus," Cancer
Biother., 1994, 9: 225-235.
[23] Y. Tzadok-David, M. Metzkin-Eizenberg, Z. Zakay-Rones. "The effect
of a mesogenic and a lentogenic Newcastle disease virus strain on
Burkitt lymphoma Daudi cells," J Cancer Res Clin Oncol., 1995, 121:
169-174.
[24] T. Ahlert and V. Schirrmacher. "Isolation of a human melanoma
adapted Newcastle disease virus mutant with highly selective
replication patterns," Cancer Res., 1990, 50: 5962-5968.
[25] P. V Ravindra, A. K. Tiwari, B. Ratta, U. Chaturvedi, S. K. Palia, P. K.
Subudhi et al. "Induction of apoptosis in Vero cells by Newcastle
disease virus requires viral replication, de-novo protein synthesis and
caspase activation," Virus Res., 2008, 133: 285-290.
[26] B. Washburn, M. A. Weigand, A. Grosse-Wilde, M. Janke, H. Stahl, E.
Rieser, M. R. Sprick, V. Schirrmacher and H. Walczak. "TNF-related
apoptosis-inducing ligand mediates tumoricidal activity of human
monocytes stimulated by Newcastle disease virus," J. Immunol., 2003,
170: 1814-1821.
[27] J. F. R. Kerr, A. H. Wyllie and A. R. Currie. "Apoptosis: a basic
biological phenomenon with wide-ranging implications in tissue
kinetics," Br. J. Cance., 1972, 26: 239-257.
[28] A. H. Wyllie, J. F. R. Kerr and A. R. Currie. "Cell death: the
significance of apoptosis," Int. Rev. Cytol., 1980, 68: 251-306.
[29] S. A. Jahanmehr, K. Hyde, C. G. Geary et al. "Simple technique for
fluorescence staining of blood cells with acridine orange," J Clin
Pathol., 1987, 40: 926-929.
[30] A. J. McGohan, S. J. Martin, R. P. Bissonette, A. Mahboubi, Y. Shi, R.
J. Mogil, W. K. Nishioka and D. R. Green, in Methods in Cell Biology,
L.M Schwartz and B.A Osborne, Eds. London: Academic Press
Incorporated. 1995, pp. 153-185.
[31] K. Mascotti, J. McCullough and S. R. Burger. "HPC viability
measurement: Trypan blue versus acridine orange and propidium
iodide," Transfusion, 2000, 40: 693-696.
[1] K. W. Reichard, R. M. Lorence, C. J. Cascino, M. E. Peeples, R. J.
Walter, M. B. Fernando, H. M. Reyes and J. A. Greager. "Newcastle
disease virus selectively kills human tumor cells," J. Surg. Res., 1992,
52: 448-453.
[2] A. R. Omar, A. Ideris, A. M. Ali, F. Othman, K. Yusoff, J. M.
Abdullah et al. "An overview of the development of Newcastle disease
virus as an anti-cancer therapy," Malaysian J Med Sci., 2002, 9: 4-12.
[3] D. J. Alexander, "Newcastle disease and other avian Paramyxoviridae
infections," in Diseases of Poultry, 10th ed., B. W. Calneck, Ed. Ames,
IA: Iowa State University Press, 1997, pp. 541-569.
[4] M. A. Mayo. 2002. "A summary of taxonomic changes recently
approved by ICTV". Arch. Virol., 2002, 147: 1655-1666.
[5] D. J. Alexander, "Newcastle disease," in A Laboratory Manual for the
Isolation and Identification of Avian Pathogens, 3rd ed., H. G.
Purchase, L. H. Arp, C. H. Domermuth and J. E.Pearson, Eds. Kennett
Square, PA: American Association of Avian Pathologists, 1989, pp.
114-120.
[6] F. M. Batliwalla, B. A. Bateman, D. Serrano, D. Murray, S. Macphail,
V. C. Maino et al. "15-year follow-up of AJCC stage III malignant
melanoma patients treated postsurgically with Newcastle disease virus
(NDV) oncolysate and determination of alterations in the CD8 T cell
Noun 1. CD8 T cell - T cell with CD8 receptor that recognizes antigens
on the surface of a virus-infected cell and binds to the infected cell and
kill itCD8 cell, cytotoxic T cell, killer cell, killer T cell repertoire,"
Mol Med., 1998, 4: 783-794.
[7] V. Schirrmacher, A. Griesbach and T. Ahlert. "Anti-tumour effects of
Newcastle Disease Virus in vivo: local versus systemic effects,"
Int J Oncol., 2001, 18 : 945-52.
[8] R. M. Lorence, A. L. Pecora, P. P. Major, S. J. Hotte, S. A. Laurie, M.
S. Roberts et al. 2003. "Overview of phase I studies of intravenous
administration of PV701, an oncolytic virus," Curr Opin Mol Ther.,
2003, 5: 618-624.
[9] P. B. Spradbrow and J. L. Samue, "Oral Newcastle disease vaccine in
experimental chickens in Australia," in Newcastle Disease in poultry,
J. W. Copland, Ed. ACIAR: Canberra, 1987, pp: 44-49. 1987.
[10] H. Westbury. "Newcastle disease virus: an evolving pathogen," Avian
Pathology, 2001, 30: 5-11.
[11] M. C. Lai and A. L. Ibrahim, "Velogenic-viscerotropic Newcastle
disease virus," in New castle disease virus in poultry, J.W. Copland Ed.
ACIAR Monograph.1987.
[12] W. S. Tan, C. H. Lau, B. K. Ng, A. L. Ibrahim and K. Yusoff.
"Nucleotide sequence of the haemagglutinin-neuraminidase (HN) gene
of a Malaysian heat resistant viscerotropic-velogenic Newcastle disease
virus," DNA Sequence, 1995, 6: 47-50.
[13] K. Yusoff and W. S. Tan. "Newcastle disease virus: macromolecules
and opportunities," Avian Pathology, 2001, 30: 439-455.
[14] M. Meyyappan. "Oncolytic effect of Newcastle disease virus on the
MCF-7 and MDA-231 breast cancer cell lines," Master Thesis,
Universiti Putra Malaysia, Malaysia, 2003.
[15] H. S. M. Wali. "The effects of Newcastle Disease Virus (NDV) on
breast cancer cell lines," Master Thesis, Universiti Putra Malaysia,
Malaysia, 2003.
[16] M. Aied. "Antileukaemic effect of local Newcastle disease virus strains
AF2240 and V4-UPM on leukaemia cell lines in vitro and in vivo," Ph.
D Thesis. Universiti Putra Malaysia, Malaysia, 2007.
[17] Rola Ali, Aied M. Alabsi, Abdul Manaf Ali, Aini Ideris , Abdul
Rahman Omar and Khatijah Yousoff. "Apoptosis Induction and
Cytolytic Effects of Newcastle Disease Virus Strain Af2240 on
DBTRG.05 mg Brain Tumor Cell Line." International Journal of
Cancer Research, 2011, 7: 25-35.
[18] D. J. Alexander, "Newcastle Disease Diagnosis," in Newcastle
Disease, D. J. Alexander, Ed. Kluwer Academic Publishers: Norwell,
Massachusetts, 1988, pp: 147-160.
[19] P. Chambers and A. C. R. Samson. "A new structural protein for
newcastle disease virus," J. General Virol.1980, 50: 155-166.
[20] K. Yusoff, W. S. Tan, C. H. Lau, B. K. Ng and A. L. Ibrahim.
"Sequence of the haemagglutinin-neuraminadase gene of the
Newcastle disease virus oral vaccine strain V4 (UPM)," Avian Pathol.,
1996, 25: 837-844.
[21] R. M. Lorence, P. A. Rood and K. W. Kelley. "Newcastle disease virus
as an antineoplastic agent: induction of tumor necrosis factor-alpha and
augmentation of its cytotoxicity," J Natl Cancer Inst., 1988, 80: 1305-
1312.
[22] U. Zorn, I. Dallmann, J. Grosse J. et al. "Induction of cytokines and
cytotoxicity against tumor cells by Newcastle disease virus," Cancer
Biother., 1994, 9: 225-235.
[23] Y. Tzadok-David, M. Metzkin-Eizenberg, Z. Zakay-Rones. "The effect
of a mesogenic and a lentogenic Newcastle disease virus strain on
Burkitt lymphoma Daudi cells," J Cancer Res Clin Oncol., 1995, 121:
169-174.
[24] T. Ahlert and V. Schirrmacher. "Isolation of a human melanoma
adapted Newcastle disease virus mutant with highly selective
replication patterns," Cancer Res., 1990, 50: 5962-5968.
[25] P. V Ravindra, A. K. Tiwari, B. Ratta, U. Chaturvedi, S. K. Palia, P. K.
Subudhi et al. "Induction of apoptosis in Vero cells by Newcastle
disease virus requires viral replication, de-novo protein synthesis and
caspase activation," Virus Res., 2008, 133: 285-290.
[26] B. Washburn, M. A. Weigand, A. Grosse-Wilde, M. Janke, H. Stahl, E.
Rieser, M. R. Sprick, V. Schirrmacher and H. Walczak. "TNF-related
apoptosis-inducing ligand mediates tumoricidal activity of human
monocytes stimulated by Newcastle disease virus," J. Immunol., 2003,
170: 1814-1821.
[27] J. F. R. Kerr, A. H. Wyllie and A. R. Currie. "Apoptosis: a basic
biological phenomenon with wide-ranging implications in tissue
kinetics," Br. J. Cance., 1972, 26: 239-257.
[28] A. H. Wyllie, J. F. R. Kerr and A. R. Currie. "Cell death: the
significance of apoptosis," Int. Rev. Cytol., 1980, 68: 251-306.
[29] S. A. Jahanmehr, K. Hyde, C. G. Geary et al. "Simple technique for
fluorescence staining of blood cells with acridine orange," J Clin
Pathol., 1987, 40: 926-929.
[30] A. J. McGohan, S. J. Martin, R. P. Bissonette, A. Mahboubi, Y. Shi, R.
J. Mogil, W. K. Nishioka and D. R. Green, in Methods in Cell Biology,
L.M Schwartz and B.A Osborne, Eds. London: Academic Press
Incorporated. 1995, pp. 153-185.
[31] K. Mascotti, J. McCullough and S. R. Burger. "HPC viability
measurement: Trypan blue versus acridine orange and propidium
iodide," Transfusion, 2000, 40: 693-696.
@article{"International Journal of Biological, Life and Agricultural Sciences:53489", author = "Siti Aishah Abu Bakar and Madihah Zawawi and Abdul Manaf Ali and Aini Ideris", title = "Induction of Apoptosis by Newcastle Disease Virus Strains AF220 and V4-UPM in Human Promyelocytic Leukemia (HL60) and Human T-Lymphoblastic Leukemia (CEM-SS) Cells", abstract = "Newcastle Disease Virus (NDV), an avian
paramyxovirus, is a highly contagious, generalised virus disease of
domestic poultry and wild birds characterized by gastro-intestinal,
respiratory and nervous signs. In this study, it was shown that NDV
strain AF2240 and V4-UPM are cytolytic to Human Promyelocytic
Leukemia, HL60 and Human T-lymphoblastic Leukemia, CEM-SS
cells. Results from MTT cytolytic assay showed that CD50 for NDV
AF2240 against HL60 was 130 HAU and NDV V4-UPM against
HL60 and CEM-SS were 110.6 and 150.9 HAU respectively.
Besides, both strains were found to inhibit the proliferation of cells in
a dose dependent manner. The mode of cell death either by apoptosis
or necrosis was further analyzed using acridine orange and propidium
iodide (AO/PI) staining. Our results showed that both NDV strains
induced primarily apoptosis in treated cells at CD50 concentration. In
conclusion, both NDV strains caused cytolytic effects primarily via
apoptosis in leukemia cells.", keywords = "Apoptosis, Cytolytic, Leukaemia, Newcastle DiseaseVirus", volume = "6", number = "4", pages = "144-5", }
