Abstract: Attempts were made to identify anuran glial cells. They were found as nervous tissue resident. Having stage dependent morphotype changes, whereby, appeared as an ovoid to oval in resting state and amoeboid mrophotypes in activated state, stained fairly with methylene blue and take up Pelikane blue 10% aqueous solution, as well as having the ability to phagocytize heat killed Staphylococcus aureus. They were delineated from the migrating peripheral monocytes by morphotypic and morphometeric differences. Such criteria were consistence with glial cells. Thus, the anuran glial cells are being identified in the frog Rana ridibunda Pallas 1771 and this animal can be of use as a simple model for the immunobiology of glial cells.
Abstract: Most CT reconstruction system x-ray computed
tomography (CT) is a well established visualization technique in
medicine and nondestructive testing. However, since CT scanning
requires sampling of radiographic projections from different viewing
angles, common CT systems with mechanically moving parts are too
slow for dynamic imaging, for instance of multiphase flows or live
animals. A large number of X-ray projections are needed to
reconstruct CT images, so the collection and calculation of the
projection data consume too much time and harmful for patient. For
the purpose of solving the problem, in this study, we proposed a
method for tomographic reconstruction of a sample from a limited
number of x-ray projections by using linear interpolation method. In
simulation, we presented reconstruction from an experimental x-ray
CT scan of a Aluminum phantom that follows to two steps: X-ray
projections will be interpolated using linear interpolation method and
using it for CT reconstruction based upon Ordered Subsets
Expectation Maximization (OSEM) method.
Abstract: Snake bite cases in Malaysia most often involve the
species Naja-naja and Calloselasma rhodostoma. In keeping with the
need for a rapid snake venom detection kit in a clinical setting, plate
and dot-ELISA test for the venoms of Naja-naja sumatrana,
Calloselasma rhodostoma and the cobra venom fraction V antigen
was developed. Polyclonal antibodies were raised and further used to
prepare the reagents for the dot-ELISA test kit which was tested in
mice, rabbit and virtual human models. The newly developed dot-
ELISA kit was able to detect a minimum venom concentration of
244ng/ml with cross reactivity of one antibody type. The dot-ELISA
system was sensitive and specific for all three snake venom types in
all tested animal models. The lowest minimum venom concentration
detectable was in the rabbit model, 244ng/ml of the cobra venom
fraction V antigen. The highest minimum venom concentration was
in mice, 1953ng/ml against a multitude of venoms. The developed
dot-ELISA system for the detection of three snake venom types was
successful with a sensitivity of 95.8% and specificity of 97.9%.
Abstract: The efficiency of chitosan beads processed from 4
marine animal shells; white leg shrimp (Litopenaeus vannamei), mud
crab (Scylla sp.), horseshoe crab (Carcinoscorpius rotundicauda),
and cuttlefish bone (Sepia sp.), for the adsorption experiments of
ammonia and formaldehyde were investigated. The porosities of
chitosan from the shells looked like beads were distinctly examined
under SEM. The original pores of those shells on the surface areas
compose of evenly fine pores. The shell beads of cuttlefish bone and
horseshoe crab show the larger probably even porosity, while on
those white leg shrimp and mud crab contain various large and fine
pores. The best adsorption at pH 9 in 18 mg/l ammonia at 2 hours
yield on cuttlefish bone, horseshoe crab, mud crab and white leg
shrimp with the average percent of 59.12, 51.45, 45.66 and 43.52,
respectively. Within 30 minutes the formaldehyde absorbers (at pH 5
in 8 μg/ml) revealed 46.27, 26.56, and 18.04 percent capacities in
cuttlefish bone, mud crab and white leg shrimp beads; while 22.44
percent in the horseshoe crab at pH 7. The adsorption capacities and
the amounts of beads showed a positive correlation. The adsorption
capacity relationship between pH and the gas concentrations were
affected by these qualities of chitosan beads.
Abstract: The increasing usage of antibiotics in the animal
farming industry is an emerging worldwide problem contributing to
the development of antibiotic resistance. The purpose of this work was
to investigate the prevalence and antibiotic resistance profile of
bacterial isolates collected from aquatic environments and meats in a
peri-urban community in Daejeon, Korea. In an antibacterial
susceptibility test, the bacterial isolates showed a high incidence of
resistance (~ 26.04 %) to cefazolin, tetracycline, gentamycin,
norfloxacin, erythromycin and vancomycin. The results from a test for
multiple antibiotic resistance indicated that the isolates were
displaying an approximately 5-fold increase in the incidence of
multiple antibiotic resistance to combinations of two different
antibiotics compared to combinations of three or more antibiotics.
Most of the isolates showed multi-antibiotic resistance, and the
resistance patterns were similar among the sampling groups.
Sequencing data analysis of 16S rRNA showed that most of the
resistant isolates appeared to be dominated by the classes
Betaproteobacteria and Gammaproteobacteria in the phylum
Proteobacteria.
Abstract: The aim of present study was to assess the effect of
glucogenic (G) and lipogenic (L) diets on blood metabolites in
Baloochi lambs. Three rumen cannulated Baloochi sheep were used
as a 3×3 Latin square design with 3 periods (28 days). Experimental
diets were a glucogenic, a lipogenic and a mixture of G and L diets
(50:50). The animals were fed diets consisted of 50% chopped alfalfa
hay and 50% concentrate. Diets were fed once daily ad libitum.
Blood samples were taken from jugular vein before the feeding, 2, 4
and 6 hour post feeding at day 27. Results indicated that β-
hydroxybutyrate (BHBA), glucose, insulin and aspartate
aminotransferase (AST) were not affected by treatments (P > 0.05).
However, lipogenic diet increased significantly activity of Alanine
aminotransferase (ALT) and concentration of non-esterified fatty acid
(NEFA) in blood plasma (P < 0.05)
Abstract: Forty-five dairy cows were used to compare the
enzyme activity of alkaline phosphatase (ALP), lactate
dehydrogenase (LDH), α -amylase in the cervical mucus of cows
during spontaneous and induced estrus using progestagen or PGF2 α
and to determine whether these enzymes affect the fertility in cows
with induced estrus, at the time of Al. The animals were assigned to 3
groups (no treatment, a Crestar® for 12 days, a double im injection of
PGF2 α). The cows were artificially inseminated (AI). Cervical
mucus samples were collected from all cows 3 to 5 min before the
AI. The results are summarized as follows: ALP and α -amylase
activity for spontaneous estrus were similar to those for induced
estrus (P>0.05) . LDH activity levels during spontaneous and PGF2 α
induced estrus was significantly lower (P < 0.001) than that in
progestagene induced estrus groups. While no difference was found
between the first and the third groups. Our result showed a significant
difference in LDH activity levels between cows conceived with 2 or
more AI and those conceived with 1 AI. The result of this study
showed that the enzyme activity in cervical mucus is helpful for
detection of ovulation and time of AI.
Abstract: This research is part of a broad program aimed at
advancing the science and technology involved in the rescue and
rehabilitation of oiled wildlife. One aspect of this research involves
the use of oil-sequestering magnetic particles for the removal of
contaminants from plumage – so-called “magnetic cleansing". This
treatment offers a number of advantages over conventional
detergent-based methods including portability - which offers the
possibility of providing a “quick clean" to the animal upon first
encounter in the field. This could be particularly advantageous
when the contaminant is toxic and/or corrosive and/or where there
is a delay in transporting the victim to a treatment centre. The
method could also be useful as part of a stabilization protocol when
large numbers of affected animals are awaiting treatment. This
presentation describes the design, development and testing of a
prototype field kit for providing a “quick clean" to contaminated
wildlife in the field.
Abstract: Recently there has been a growing interest in the field
of bio-mimetic robots that resemble the behaviors of an insect or an
aquatic animal, among many others. One of various bio-mimetic robot
applications is to explore pipelines, spotting any troubled areas or
malfunctions and reporting its data. Moreover, the robot is able to
prepare for and react to any abnormal routes in the pipeline. Special
types of mobile robots are necessary for the pipeline monitoring tasks.
In order to move effectively along a pipeline, the robot-s movement
will resemble that of insects or crawling animals. When situated in
massive pipelines with complex routes, the robot places fixed sensors
in several important spots in order to complete its monitoring. This
monitoring task is to prevent a major system failure by preemptively
recognizing any minor or partial malfunctions. Areas uncovered by
fixed sensors are usually impossible to provide real-time observation
and examination, and thus are dependent on periodical offline
monitoring. This paper proposes a monitoring system that is able to
monitor the entire area of pipelines–with and without fixed
sensors–by using the bio-mimetic robot.
Abstract: Artemia is one of the most conspicuous invertebrates
associated with aquaculture. It can be considered as a model
organism, offering numerous advantages for comprehensive and
multidisciplinary studies using morphologic or molecular methods.
Since DNA extraction is an important step of any molecular
experiment, a new and a rapid method of DNA extraction from adult
Artemia was described in this study. Besides, the efficiency of this
technique was compared with two widely used alternative techniques,
namely Chelex® 100 resin and SDS-chloroform methods. Data
analysis revealed that the new method is the easiest and the most cost
effective method among the other methods which allows a quick and
efficient extraction of DNA from the adult animal.
Abstract: Delayed wound healing in diabetes is primarily
associated with hyperglycemia, over-expression of inflammatory
marker, oxidative stress and delayed collagen synthesis. This
unmanaged wound is producing high economic burden on the
society. Thus research is required to develop new and effective
treatment strategies to deal with this emerging issue. Our present
study incorporates the evaluation of wound healing effects of 50%
ethanol extract of Ocimum sanctum (OSE) in streptozotocin
(45mg/kg)-induced diabetic rats with concurrent wound ulcer. The
animals showing diabetes (Blood glucose level >140 and
Abstract: There has been a growing interest in the field of
bio-mimetic robots that resemble the shape of an insect or an aquatic
animal, among many others. One bio-mimetic robot serves the
purpose of exploring pipelines, spotting any troubled areas or
malfunctions and reporting its data. Moreover, the robot is able to
prepare for and react to any abnormal routes in the pipeline. In order
to move effectively inside a pipeline, the robot-s movement will
resemble that of a lizard. When situated in massive pipelines with
complex routes, the robot places fixed sensors in several important
spots in order to complete its monitoring. This monitoring task is to
prevent a major system failure by preemptively recognizing any minor
or partial malfunctions. Areas uncovered by fixed sensors are usually
impossible to provide real-time observation and examination, and thus
are dependant on periodical offline monitoring. This paper provides
the Monitoring System that is able to monitor the entire area of
pipelines–with and without fixed sensors–by using the bio-mimetic
robot.
Abstract: Data on 657 lactation from 163 Maltese goat,
collected over a 5-year period were analyzed by a mixed model to
estimate the variance components for heritability. The considered
lactation traits were: milk yield (MY) and lactation length (LL). Year,
parity and type of birth (single or twin) were significant sources of
variation for lactation length; on the other hand milk yield was
significantly influenced only by the year. The average MY was
352.34 kg and the average LL was 230 days. Estimates of heritability
were 0.21 and 0.15 for MY and LL respectively. These values
suggest there is low correlation between genotype and phenotype so
it may be difficult to evaluate animals directly on phenotype. So, the
genetic improvement of this breed may be quite slow without the
support of progeny test aimed to select Maltese breeders.
Abstract: The presence of heavy metals in the environment
could constitute a hazard to food security and public health. These
can be accumulated in aquatic animals such as fish. Samples of four
popular brands of canned fish in the Iranian market (yellowfin tuna,
common Kilka, Kawakawa and longtail tuna) were analyzed for level
of Cr after wet digestion with acids using graphite furnace atomic
absorption spectrophotometry. The mean concentrations for Cr in the
different brands were: 2.57, 3.24, 3.16 and 1.65 μg/g for brands A, B,
C and D respectively. Significant differences were observed in the Cr
levels between all of the different brands of canned fish evaluated in
this study. The Cr concentrations for the varieties of canned fishes
were generally within the FAO/WHO, U.S. FDA and U.S. EPA
recommended limits for fish.
Abstract: This article introduces the actual problem that is а
study of proposed by the authors Hydrocele ointment in amino acids’
metabolism of cows’ blood in inflammation of traumatic origin.
Hydrocele ointment has shown a positive effect on inflammatory
process and amino acids’ metabolism of animals treated with the
drug. Amino acid levels reached physiological parameters on the 10th
day after treatment; in the control group this parameter was higher
than normal.
Abstract: Field experiment was conducted to investigate the combine use of animal manure and inorganic fertilizer on growth and yield performance of vegetable cowpea. The experiment was laid out in a Randomized Complete Block Design (RCBD) with seven treatments. Poultry manure, cattle manure and goat manure were evaluated with recommended level of inorganic fertilizer for vegetable cowpea. The highest crop yield was obtained by the application of poultry manure combined with the recommended level of inorganic fertilizer. The lowest yield was obtained by the application of goat manure only. In addition, the results revealed that the goat manure and cattle manure were inferior to poultry manure as a source of organic manure for vegetable cowpea cultivation. The animal manure combine with chemical fertilizer gave a higher yield when compared to the sole application of animal manure. The soil analysis showed that the nitrogen content and phosphorus content of poultry manure treated plots were higher than other treatments tested. But potassium content was higher in goat manure treated plots. The results further revealed that the poultry manure has a beneficial effect on crop growth and yield compared with other treatments. Therefore, the combined use of poultry manure with inorganic fertilizer application has been recognized as the most suitable way of ensuring high crop yield.
Abstract: Sexual behavior and semen charactertistics were
evaluated in young male Boer goats in tropical condition during time
period of September to November 2009. The animal was let to have
adaptation for five months after importation from Australian climate.
A total of 20 bucks were observed for sexual behavior and ability of
semen production. Out of this number, 4 faild to libido and 3
produced poor semen. The remaing 13 animals were divided into
three groups according to the ages (11-13, 15-16 and 18-25 months).
Sexual behavior consisting response time to female teaser,
ejaculation time, fixing strenght to female and erection status were
normaly observer in 13 bucks, and there was no significant difference
between age groups. Semen characteristics from 13 bucks were in
normal quality in the volume, sperm mass motility, individual
motility, percentage of live- and abnormal sperm. We concluded that
is possible to collect semen of Boer goats during the period of
September to November under tropical condition. Collection during
other time period should be analyzed.
Abstract: The lubricating properties of commercially available
mucins originating from different animal organs, namely bovine
submaxillary mucin (BSM) and porcine gastric mucin (PGM), have
been characterized at polymeric surfaces for biomedical applications.
Atomic force microscopy (AFM) and pin-on-disk tribometry have
been employed for tribological studies at nanoscale and macroscale
contacts, respectively. Polystyrene (PS) was employed to represent
‘rigid’ contacts, whereas poly(dimethylsiloxane) (PDMS) was
employed to represent ‘soft contacts’. To understand the lubricating
properties of mucins in correlation with the coverage on surfaces,
adsorption properties of mucins onto the polymeric substrates have
been characterized by means of optical waveguide light-mode
spectroscopy (OWLS). Both mucins showed facile adsorption onto
both polymeric substrates, but the lubricity was highly dependent
upon the pH change between 2 and 7.
Abstract: To measure the thickness of the subcutaneous adipose
tissue layer, a non-invasive optical measurement system (λ=1300 nm)
is introduced. Animal and human subjects are used for the
experiments. The results of human subjects are compared with the data
of ultrasound device measurements, and a high correlation (r=0.94 for
n=11) is observed. There are two modes in the corresponding signals
measured by the optical system, which can be explained by
two-layered and three-layered tissue models. If the target tissue is
thinner than the critical thickness, detected data using diffuse
reflectance method follow the three-layered tissue model, so the data
increase as the thickness increases. On the other hand, if the target
tissue is thicker than the critical thickness, the data follow the
two-layered tissue model, so they decrease as the thickness increases.
Abstract: There is an ongoing controversy in the literature related
to the biological effects of weak, low frequency electromagnetic
fields. The physical arguments and interpretation of the experimental
evidence are inconsistent, where some physical arguments and
experimental demonstrations tend to reject the likelihood of any
effect of the fields at extremely low level. The problem arises of
explaining, how the low-energy influences of weak magnetic fields
can compete with the thermal and electrical noise of cells at normal
temperature using the theoretical studies. The magnetoreception in
animals involve radical pair mechanism. The same mechanism has
been shown to be involved in the circadian rhythm synchronization in
mammals. These reactions can be influenced by the weak magnetic
fields. Hence, it is postulated the biological clock can be affected
by weak magnetic fields and these disruptions to the rhythm can
cause adverse biological effects. In this paper, likelihood of altering
the biological clock via the radical pair mechanism is analyzed to
simplify these studies of controversy.