Enzymes Activity in Bovine Cervical Mucus Related to the Time of Ovulation And Insemination
Forty-five dairy cows were used to compare the
enzyme activity of alkaline phosphatase (ALP), lactate
dehydrogenase (LDH), α -amylase in the cervical mucus of cows
during spontaneous and induced estrus using progestagen or PGF2 α
and to determine whether these enzymes affect the fertility in cows
with induced estrus, at the time of Al. The animals were assigned to 3
groups (no treatment, a Crestar® for 12 days, a double im injection of
PGF2 α). The cows were artificially inseminated (AI). Cervical
mucus samples were collected from all cows 3 to 5 min before the
AI. The results are summarized as follows: ALP and α -amylase
activity for spontaneous estrus were similar to those for induced
estrus (P>0.05) . LDH activity levels during spontaneous and PGF2 α
induced estrus was significantly lower (P < 0.001) than that in
progestagene induced estrus groups. While no difference was found
between the first and the third groups. Our result showed a significant
difference in LDH activity levels between cows conceived with 2 or
more AI and those conceived with 1 AI. The result of this study
showed that the enzyme activity in cervical mucus is helpful for
detection of ovulation and time of AI.
[1] G. I. Gorodeski, "Estrogen increases the permeability of the cultured
human cervical epithelium by modulating cell deformability". Am J
Physiol Cell Physiol., pp. 275-888-899. 1998.
[2] K. S. Moghiss, and R. J. Blandau, "Biology of the cervix". J. reprod.
Med., pp. 8, 21. 1972
[3] Th. Tsiligianni, A. Karagiannidis, P. Brikas, and Ph. Saratsis,. "Enzyme
activity in bovine cervical mucus during spontaneous and induced estrus
by progesterone and/or PGF2╬▒". The canadian journal of veterinary
research., Vol 67, pp. 189-193. 2003.
[4] J. P. W. Vermeiden, R. E. Bernardus, C. S. Ten Brug, C. H. Statema-
Lohmeijer, A. M. Willemsen-Brugma, and J. Schoemaker, "Pregnancy
rate is significantly higher in vitro fertilization procedure with
spermatozoa isolated from nonliquefying semen in which liquefaction is
induced by a-amylase". Fertil Steril, Vol. 51, pp. 149-152. 1989
[5] L. J. Boyd, A. R. Gibbons, and J. B. Tasker, "Characteristics of cervical
mucus from progestagen-treated cattle". Br Vet J, Vol. 128. PP. 260-
269. 1972.
[6] A. Prasad, N. R. Kalalyan, N. K. Bachlaus, R. C. Arora,and R. S.
Pandey, "Biochemical changes in the cervical mucus of buffalo after
induction of oestrus with prostaglandin F2 and cloprostenol". J Reprod
Fertil, Vol. 62, pp. 583-587. 1981.
[7] T. Takehisa, "Lactate dehydrogenase in human cervical mucus:
correlation with ovulation, influence of ovarian steroid hormones, and
isozyme pattern". Fertil Steril, Vol. 33, no. 2, pp. 135-140. 1980.
[8] C. Treves, M. T. Vincenzini, P. Vanni, F. Bardazzi, A. Cattaneo, and E.
Ogier, "Changes in enzyme levels in human cervical mucus during the
menstrual cycle". Int J Fertil, Vol. 31, pp. 59-66. 1986.
[9] M. Skerlavay, J. A. Epstein, and A. J. Sobrero, "Cervical mucus
amylase levels in normal menstrual cycles". Fertil Steril, Vol. 19, pp.
726-730. 1968.
[10] N. K. Bachlaus, R. Arora, A. Prasad and R. S. Pandey, "Synchronization
of oestrus in buffalo heifers with prostaglandin F-2a, its effect on plasma
estrogen and progesterone levels". Ed. G. P. Talwar. Elsevier/North
Holland Biomedicai Press, Amsterdam. In Advances in Reproduction
and Regulation of Fertility, pp. 149- 153. 1979.
[11] S. W. Clark, and J. M. Yochim, "Effect of ovarian steroids on lactic
dehydrogenase activity in endometrium and myometrium of rat uterus".
Endocrinology, in press. 1971.
[1] G. I. Gorodeski, "Estrogen increases the permeability of the cultured
human cervical epithelium by modulating cell deformability". Am J
Physiol Cell Physiol., pp. 275-888-899. 1998.
[2] K. S. Moghiss, and R. J. Blandau, "Biology of the cervix". J. reprod.
Med., pp. 8, 21. 1972
[3] Th. Tsiligianni, A. Karagiannidis, P. Brikas, and Ph. Saratsis,. "Enzyme
activity in bovine cervical mucus during spontaneous and induced estrus
by progesterone and/or PGF2╬▒". The canadian journal of veterinary
research., Vol 67, pp. 189-193. 2003.
[4] J. P. W. Vermeiden, R. E. Bernardus, C. S. Ten Brug, C. H. Statema-
Lohmeijer, A. M. Willemsen-Brugma, and J. Schoemaker, "Pregnancy
rate is significantly higher in vitro fertilization procedure with
spermatozoa isolated from nonliquefying semen in which liquefaction is
induced by a-amylase". Fertil Steril, Vol. 51, pp. 149-152. 1989
[5] L. J. Boyd, A. R. Gibbons, and J. B. Tasker, "Characteristics of cervical
mucus from progestagen-treated cattle". Br Vet J, Vol. 128. PP. 260-
269. 1972.
[6] A. Prasad, N. R. Kalalyan, N. K. Bachlaus, R. C. Arora,and R. S.
Pandey, "Biochemical changes in the cervical mucus of buffalo after
induction of oestrus with prostaglandin F2 and cloprostenol". J Reprod
Fertil, Vol. 62, pp. 583-587. 1981.
[7] T. Takehisa, "Lactate dehydrogenase in human cervical mucus:
correlation with ovulation, influence of ovarian steroid hormones, and
isozyme pattern". Fertil Steril, Vol. 33, no. 2, pp. 135-140. 1980.
[8] C. Treves, M. T. Vincenzini, P. Vanni, F. Bardazzi, A. Cattaneo, and E.
Ogier, "Changes in enzyme levels in human cervical mucus during the
menstrual cycle". Int J Fertil, Vol. 31, pp. 59-66. 1986.
[9] M. Skerlavay, J. A. Epstein, and A. J. Sobrero, "Cervical mucus
amylase levels in normal menstrual cycles". Fertil Steril, Vol. 19, pp.
726-730. 1968.
[10] N. K. Bachlaus, R. Arora, A. Prasad and R. S. Pandey, "Synchronization
of oestrus in buffalo heifers with prostaglandin F-2a, its effect on plasma
estrogen and progesterone levels". Ed. G. P. Talwar. Elsevier/North
Holland Biomedicai Press, Amsterdam. In Advances in Reproduction
and Regulation of Fertility, pp. 149- 153. 1979.
[11] S. W. Clark, and J. M. Yochim, "Effect of ovarian steroids on lactic
dehydrogenase activity in endometrium and myometrium of rat uterus".
Endocrinology, in press. 1971.
@article{"International Journal of Biological, Life and Agricultural Sciences:52846", author = "S. Benbia and A.Kalla and M. Yahia and K. Belhadi and A. Zidani", title = "Enzymes Activity in Bovine Cervical Mucus Related to the Time of Ovulation And Insemination", abstract = "Forty-five dairy cows were used to compare the
enzyme activity of alkaline phosphatase (ALP), lactate
dehydrogenase (LDH), α -amylase in the cervical mucus of cows
during spontaneous and induced estrus using progestagen or PGF2 α
and to determine whether these enzymes affect the fertility in cows
with induced estrus, at the time of Al. The animals were assigned to 3
groups (no treatment, a Crestar® for 12 days, a double im injection of
PGF2 α). The cows were artificially inseminated (AI). Cervical
mucus samples were collected from all cows 3 to 5 min before the
AI. The results are summarized as follows: ALP and α -amylase
activity for spontaneous estrus were similar to those for induced
estrus (P>0.05) . LDH activity levels during spontaneous and PGF2 α
induced estrus was significantly lower (P < 0.001) than that in
progestagene induced estrus groups. While no difference was found
between the first and the third groups. Our result showed a significant
difference in LDH activity levels between cows conceived with 2 or
more AI and those conceived with 1 AI. The result of this study
showed that the enzyme activity in cervical mucus is helpful for
detection of ovulation and time of AI.", keywords = "cervical mucus, dairy cow, enzyme, induced, estrus,ovulation, AI", volume = "5", number = "11", pages = "668-3", }