A New Method for Rapid DNA Extraction from Artemia (Branchiopoda, Crustacea)
Artemia is one of the most conspicuous invertebrates
associated with aquaculture. It can be considered as a model
organism, offering numerous advantages for comprehensive and
multidisciplinary studies using morphologic or molecular methods.
Since DNA extraction is an important step of any molecular
experiment, a new and a rapid method of DNA extraction from adult
Artemia was described in this study. Besides, the efficiency of this
technique was compared with two widely used alternative techniques,
namely Chelex® 100 resin and SDS-chloroform methods. Data
analysis revealed that the new method is the easiest and the most cost
effective method among the other methods which allows a quick and
efficient extraction of DNA from the adult animal.
[1] J. Dhont, P. Sorgeloos, Applications of Artemia. In: Abatzopoulos, T.,
Beardmore, J., Clegg, J., Sorgeloos, P. (Eds). Artemia: basic and applied
biology. Kluwer Academic Publishers, Dordrecht, the Netherlands.
2002. 251-277.
[2] G. Gajardo, T.J. Abatzopoulos, I. Kappas, J. A. Beardmore, Chapter V.
Evolution and speciation. In: Abatzopoulos, T.J., Beardmore, J.A.,
Clegg, J.S., Sorgeloos, P. (Eds).Artemia: basic and applied biology.
Kluwer Academic Publishers, Dordrecht, the Netherlands. 2002. 225-
250.
[3] J.A. Moorad, M.S. Mayer, M.A. Simovich, Extraction of DNA from
anostracan cysts (Crustacea, Branchiopoda) for use in RAPD-PCR
analysis. Hydrobiologia. 1997.359: 159-162.
[4] P. Bossier, W. Xiaomei, F. Catania, F. Dooms, G. Van Stappen, E.
Naessens, P. Sorgeloos, An RFLP database for authentication of
commercial cyst samples of the brine shrimp Artemia spp. (International
Study on Artemia LXX). Aquaculture. 2004. 231: 93-112.
[5] S. Yu, J. Geng, P. Zhou, J. Wang, X. Chen, J. Hu, New hydroxyapatite
monolithic column for DNA extraction and its application in the
purification of Bacillus subtilis crude lysate. Journal of Chromatography
A. 2008. 1183: 29-37.
[6] C.W. Kan, C.P. Fredlake, E.A.S. Doherty, A.E. Barron, DNA
sequencing and genotyping in miniaturized electrophoresis systems,
Electrophoresis. 2004. 25, 3564-3588.
[7] H.L. Chang, Y.S. Moo, S.J. Kih, Y.E. Tae, L. Won, A chelating resin
containing 4-(2-thiazolylazo) resorcinol as the functional group.
Chromatographic application to the preconcentration and separation of
some trace metal ions including uranium. Analytical chemistry. Acta.
1997. 57-63, 351.
[8] M. Kumar, D.P.S. Rathore, A.K. Singh, Pyrogallol immobilized
Amberlite XAD-2: a newly designed collector for enrichment of metal
ions prior to their determination by flame atomic absorption
spectrometry. Microchim Acta. 2001. 137: 127.
[9] I. Singh, R. Saini, A highly selective photometric method for uranium
(VI) using pyrimidyl azo dye in presence of EDTA. Talanta. 1994. 41:
2173-2175.
[10] D. F. Boltz, J. Edward, Jr. Havlena, The ultraviolet spectrophotometric
determination of cadmium by the diethyldithiocarbamate method.
Analytical chemistry. Acta. 1964. 30: 565-568.
[11] H.W. Chen, J.C. Jin, Y.F. Wang, Flow injection on-line coprecipitation
preconcentration system using copper (II), Diethyl Dithiocarbamate as
carrier for flame atomic absorption spectrometric determination of
cadium, lead and nickel in environmental samples, Analytical chemistry.
Acta. 1997. 353: pp. 181-188.
[12] S. Dadfarina, M. Talebi, A. M. Haji Shabani, Z. A. Beni, Determination
of lead and cadmium in different samples by flow injection atomic
obsorption spectrometry incorporating a microcolumn of immobilized
ammonium pyrrolidine ditheiocarbamate on microcrystalline
naphthalene, Croat. Chemistry . Acta. 2007. 80: 17-23.
[13] J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular cloning: A laboratory
Manual, 2nd edition, cold spring Harbor Laboratory press, cold spring
Harbor, New York. 1989.
[14] P. S. Walsh, D. A. Metzger, R. Higuchi, Chelex-100 as a medium for
simple extraction of DNA for PCR-based typing from forensic material.
BioTechniques. 1991. 10: 506-513.
[15] A. Estoup, CR. Largiader, E. Perrot, D. Chourrout, Rapid one-tube DNA
extraction for reliable PCR detection of fish polymorphic markers and
transgenes. Molecular Marine Biology and Biotechnology. 1996. 5: 295-
298.
[16] L. G. Davis, M. D. Dibner, J. F. Battey, Basic Methods in Molecular
Biology. Elsevier Science Publishing Co., Inc., New York. 1986. 388
pp.
[17] A. Di Pinto, V. T. Forte, M. G. Guastadisegni, C. Martino, F. Schena, B.
Tantillo, A comparison of DNA extraction methods for food analysis.
Food Control. 2007. 18, 76-81.
[18] R. Boom, C. J. A. Sol, M. M. M. Salimans, C. L. Jansen, P. M. E.
Wertheim-Van Dillen, J. Van der Noordaa, Rapid and simple method
for purification of nucleic acids. Journal of Clinical Microbiology. 1990.
28: 495-503.
[19] L. Rudbeck, J. Dissing, Rapid, simple alkaline extraction of human
genomic DNA from whole blood, buccal epithelial cells, semen and
forensic stains for PCR. BioTechniques. 1998. 25: 588-592.
[20] G. E. Truett, P. Heeger, R. L. Mynatt, A. A. Truett, J. A. Walker, M. L.
Warman, Preparation of PCR-quality mouse genomic DNA with hot
sodium hydroxide and Tris (HotSHOT). BioTechniques. 2000. 29: 52-
54.
[21] Z. Xin, J.P. Velten, M. J. Oliver, J.J. Burke, High throughput DNA
extraction method suitable for PCR. BioTechniques. 2003. 34: 820-826.
[22] J. Montero-Pau, A. G├│mez, J. Mu├▒oz, Applification of an inexpensive
and high-throughput genomic DNA extraction method for the molecular
ecology of zooplanktonic diapausing eggs. Limnology and
Oceanography: Methods. 2008. 6: 218-222.
[23] L. De Meester, A. G├│mez, B. Okamura, K. Schwenk, Acta Oecol. The
Monopolization Hypothesis and the dispersal-gene flow paradox in
aquatic organisms. Acta Oecol. International Journal of Ecology. 2002.
23: 121-135.
[24] A. G├│mez, Molecular ecology of rotifers: from population
differentiation to speciation. Hydrobiologia. 2005. 546: 83-99.
[25] S. Ishida, D. J. Taylor, Mature habitats associated with genetic
divergence despite strong dispersal ability in an arthropod. BMC
Evolutionary Biology. 2007. 7: 52.
[26] S. J. Adamowicz, A. Purvis, How many branchiopod crustacean species
are there? Quantifying the componentsof underestimation. Global
Ecology. Biogeogr. 2005. 14: 455-468.
[27] E. Kejnovsky, J. Kypr, DNA extraction by zinc. Nucleic Acids
Research, Oxford University Press. 1997. Vol 25, Issue 9: 1870-1871.
[1] J. Dhont, P. Sorgeloos, Applications of Artemia. In: Abatzopoulos, T.,
Beardmore, J., Clegg, J., Sorgeloos, P. (Eds). Artemia: basic and applied
biology. Kluwer Academic Publishers, Dordrecht, the Netherlands.
2002. 251-277.
[2] G. Gajardo, T.J. Abatzopoulos, I. Kappas, J. A. Beardmore, Chapter V.
Evolution and speciation. In: Abatzopoulos, T.J., Beardmore, J.A.,
Clegg, J.S., Sorgeloos, P. (Eds).Artemia: basic and applied biology.
Kluwer Academic Publishers, Dordrecht, the Netherlands. 2002. 225-
250.
[3] J.A. Moorad, M.S. Mayer, M.A. Simovich, Extraction of DNA from
anostracan cysts (Crustacea, Branchiopoda) for use in RAPD-PCR
analysis. Hydrobiologia. 1997.359: 159-162.
[4] P. Bossier, W. Xiaomei, F. Catania, F. Dooms, G. Van Stappen, E.
Naessens, P. Sorgeloos, An RFLP database for authentication of
commercial cyst samples of the brine shrimp Artemia spp. (International
Study on Artemia LXX). Aquaculture. 2004. 231: 93-112.
[5] S. Yu, J. Geng, P. Zhou, J. Wang, X. Chen, J. Hu, New hydroxyapatite
monolithic column for DNA extraction and its application in the
purification of Bacillus subtilis crude lysate. Journal of Chromatography
A. 2008. 1183: 29-37.
[6] C.W. Kan, C.P. Fredlake, E.A.S. Doherty, A.E. Barron, DNA
sequencing and genotyping in miniaturized electrophoresis systems,
Electrophoresis. 2004. 25, 3564-3588.
[7] H.L. Chang, Y.S. Moo, S.J. Kih, Y.E. Tae, L. Won, A chelating resin
containing 4-(2-thiazolylazo) resorcinol as the functional group.
Chromatographic application to the preconcentration and separation of
some trace metal ions including uranium. Analytical chemistry. Acta.
1997. 57-63, 351.
[8] M. Kumar, D.P.S. Rathore, A.K. Singh, Pyrogallol immobilized
Amberlite XAD-2: a newly designed collector for enrichment of metal
ions prior to their determination by flame atomic absorption
spectrometry. Microchim Acta. 2001. 137: 127.
[9] I. Singh, R. Saini, A highly selective photometric method for uranium
(VI) using pyrimidyl azo dye in presence of EDTA. Talanta. 1994. 41:
2173-2175.
[10] D. F. Boltz, J. Edward, Jr. Havlena, The ultraviolet spectrophotometric
determination of cadmium by the diethyldithiocarbamate method.
Analytical chemistry. Acta. 1964. 30: 565-568.
[11] H.W. Chen, J.C. Jin, Y.F. Wang, Flow injection on-line coprecipitation
preconcentration system using copper (II), Diethyl Dithiocarbamate as
carrier for flame atomic absorption spectrometric determination of
cadium, lead and nickel in environmental samples, Analytical chemistry.
Acta. 1997. 353: pp. 181-188.
[12] S. Dadfarina, M. Talebi, A. M. Haji Shabani, Z. A. Beni, Determination
of lead and cadmium in different samples by flow injection atomic
obsorption spectrometry incorporating a microcolumn of immobilized
ammonium pyrrolidine ditheiocarbamate on microcrystalline
naphthalene, Croat. Chemistry . Acta. 2007. 80: 17-23.
[13] J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular cloning: A laboratory
Manual, 2nd edition, cold spring Harbor Laboratory press, cold spring
Harbor, New York. 1989.
[14] P. S. Walsh, D. A. Metzger, R. Higuchi, Chelex-100 as a medium for
simple extraction of DNA for PCR-based typing from forensic material.
BioTechniques. 1991. 10: 506-513.
[15] A. Estoup, CR. Largiader, E. Perrot, D. Chourrout, Rapid one-tube DNA
extraction for reliable PCR detection of fish polymorphic markers and
transgenes. Molecular Marine Biology and Biotechnology. 1996. 5: 295-
298.
[16] L. G. Davis, M. D. Dibner, J. F. Battey, Basic Methods in Molecular
Biology. Elsevier Science Publishing Co., Inc., New York. 1986. 388
pp.
[17] A. Di Pinto, V. T. Forte, M. G. Guastadisegni, C. Martino, F. Schena, B.
Tantillo, A comparison of DNA extraction methods for food analysis.
Food Control. 2007. 18, 76-81.
[18] R. Boom, C. J. A. Sol, M. M. M. Salimans, C. L. Jansen, P. M. E.
Wertheim-Van Dillen, J. Van der Noordaa, Rapid and simple method
for purification of nucleic acids. Journal of Clinical Microbiology. 1990.
28: 495-503.
[19] L. Rudbeck, J. Dissing, Rapid, simple alkaline extraction of human
genomic DNA from whole blood, buccal epithelial cells, semen and
forensic stains for PCR. BioTechniques. 1998. 25: 588-592.
[20] G. E. Truett, P. Heeger, R. L. Mynatt, A. A. Truett, J. A. Walker, M. L.
Warman, Preparation of PCR-quality mouse genomic DNA with hot
sodium hydroxide and Tris (HotSHOT). BioTechniques. 2000. 29: 52-
54.
[21] Z. Xin, J.P. Velten, M. J. Oliver, J.J. Burke, High throughput DNA
extraction method suitable for PCR. BioTechniques. 2003. 34: 820-826.
[22] J. Montero-Pau, A. G├│mez, J. Mu├▒oz, Applification of an inexpensive
and high-throughput genomic DNA extraction method for the molecular
ecology of zooplanktonic diapausing eggs. Limnology and
Oceanography: Methods. 2008. 6: 218-222.
[23] L. De Meester, A. G├│mez, B. Okamura, K. Schwenk, Acta Oecol. The
Monopolization Hypothesis and the dispersal-gene flow paradox in
aquatic organisms. Acta Oecol. International Journal of Ecology. 2002.
23: 121-135.
[24] A. G├│mez, Molecular ecology of rotifers: from population
differentiation to speciation. Hydrobiologia. 2005. 546: 83-99.
[25] S. Ishida, D. J. Taylor, Mature habitats associated with genetic
divergence despite strong dispersal ability in an arthropod. BMC
Evolutionary Biology. 2007. 7: 52.
[26] S. J. Adamowicz, A. Purvis, How many branchiopod crustacean species
are there? Quantifying the componentsof underestimation. Global
Ecology. Biogeogr. 2005. 14: 455-468.
[27] E. Kejnovsky, J. Kypr, DNA extraction by zinc. Nucleic Acids
Research, Oxford University Press. 1997. Vol 25, Issue 9: 1870-1871.
@article{"International Journal of Biological, Life and Agricultural Sciences:52386", author = "R. Manaffar and R. Maleki and S. Zare and N. Agh and S. Soltanian and B. Sehatnia and P. Sorgeloos and P. Bossier and G. Van Stappen", title = "A New Method for Rapid DNA Extraction from Artemia (Branchiopoda, Crustacea)", abstract = "Artemia is one of the most conspicuous invertebrates
associated with aquaculture. It can be considered as a model
organism, offering numerous advantages for comprehensive and
multidisciplinary studies using morphologic or molecular methods.
Since DNA extraction is an important step of any molecular
experiment, a new and a rapid method of DNA extraction from adult
Artemia was described in this study. Besides, the efficiency of this
technique was compared with two widely used alternative techniques,
namely Chelex® 100 resin and SDS-chloroform methods. Data
analysis revealed that the new method is the easiest and the most cost
effective method among the other methods which allows a quick and
efficient extraction of DNA from the adult animal.", keywords = "APD, Artemia, DNA extraction, Molecularexperiments", volume = "4", number = "1", pages = "31-5", }
