Abstract: Adult mesenchymal stem cells (MSCs) have been investigated using preclinical approaches for tissue regeneration. Porcine MSCs (pMSCs) are capable of growing and attaching to plastic with a fibroblast-like morphology and then differentiating into bone, adipose, and cartilage tissues in vitro. This study was conducted to investigate the proliferating abilities, differentiation potentials, and multipotency of miniature pig adipose tissue-derived MSCs (mpAD-MSCs) with or without long-term cryopreservation, considering that cryostorage has the potential for use in clinical applications. After confirming the characteristics of the mpAD-MSCs, we examined the effect of long-term cryopreservation (> 2 years) on expression of cell surface markers (CD34, CD90 and CD105), proliferating abilities (cumulative population doubling level, doubling time, colony-forming unit, and MTT assay) and differentiation potentials into mesodermal cell lineages. As a result, the expression of cell surface markers is similar between thawed and fresh mpAD-MSCs. However, long-term cryopreservation significantly lowered the differentiation potentials (adipogenic, chondrogenic, and osteogenic) of mpAD-MSCs. When compared with fresh mpAD-MSCs, thawed mpAD-MSCs exhibited lower expression of mesodermal cell lineage-related genes such as peroxisome proliferator-activated receptor-g2, lipoprotein lipase, collagen Type II alpha 1, osteonectin, and osteocalcin. Interestingly, long-term cryostoraged mpAD-MSCs exhibited significantly higher cell viability than the fresh mpAD-MSCs. Long-term cryopreservation induced a 30% increase in the cell viability of mpAD-MSCs when compared with the fresh mpAD-MSCs at 5 days after thawing. However, long-term cryopreservation significantly lowered expression of stemness markers such as Oct3/4, Sox2, and Nanog. Furthermore, long-term cryopreservation negatively affected expression of senescence-associated genes such as telomerase reverse transcriptase and heat shock protein 90 of mpAD-MSCs when compared with the fresh mpAD-MSCs. The results from this study might be important for the successful application of MSCs in clinical trials after long-term cryopreservation.
Abstract: Asphalt pavement itself is a mixture made up of mainly aggregates, binders, and fillers that acts as a composition used for pavement construction. An experimental program was setup to determine the fatigue performance test of Asphalt with three different grades of conventional binders. Asphalt specimen has achieved the maximum optimum bulk density and air voids with a consistent bulk density of 2.3 t/m3, with an air void of 5% ± 0.5, before loading into the Asphalt Mixture Performance Tested (AMPT) for fatigue test. The number of cycles is defined as the point where phase angle drops, which is caused by the formation of cracks due to the increasing micro cracks when asphalt is undergoing repeated cycles of loading. Thus, the data collected are analyzed using the drop of phase angle as failure criteria. Based in the data analyzed, it is evident that the fatigue life of asphalt lies on the grade of binder. The result obtained shows that all specimens do experience a drop in phase angle due to macro cracks in the asphalt specimen.
Abstract: This paper outlines the development of an
experimental technique in quantifying supersonic jet flows, in an
attempt to avoid seeding particle problems frequently associated with
particle-image velocimetry (PIV) techniques at high Mach numbers.
Based on optical flow algorithms, the idea behind the technique
involves using high speed cameras to capture Schlieren images of the
supersonic jet shear layers, before they are subjected to an adapted
optical flow algorithm based on the Horn-Schnuck method to
determine the associated flow fields. The proposed method is capable
of offering full-field unsteady flow information with potentially
higher accuracy and resolution than existing point-measurements or
PIV techniques. Preliminary study via numerical simulations of a
circular de Laval jet nozzle successfully reveals flow and shock
structures typically associated with supersonic jet flows, which serve
as useful data for subsequent validation of the optical flow based
experimental results. For experimental technique, a Z-type Schlieren
setup is proposed with supersonic jet operated in cold mode,
stagnation pressure of 4 bar and exit Mach of 1.5. High-speed singleframe
or double-frame cameras are used to capture successive
Schlieren images. As implementation of optical flow technique to
supersonic flows remains rare, the current focus revolves around
methodology validation through synthetic images. The results of
validation test offers valuable insight into how the optical flow
algorithm can be further improved to improve robustness and
accuracy. Despite these challenges however, this supersonic flow
measurement technique may potentially offer a simpler way to
identify and quantify the fine spatial structures within the shock shear
layer.
Abstract: This paper proposes an APPLE scheme that aims at providing absolute and proportional throughput guarantees, and maximizing system throughput simultaneously for wireless LANs with homogeneous and heterogenous traffic. We formulate our objectives as an optimization problem, present its exact and approximate solutions, and prove the existence and uniqueness of the approximate solution. Simulations validate that APPLE scheme is accurate, and the approximate solution can well achieve the desired objectives already.
Abstract: Energy consumption of a hotel can be a hot topic in
smart city; it is difficult to evaluate the contribution of impact factors
to energy consumption of a hotel. Therefore, grasping the key impact
factors has great effect on the energy saving management of a hotel.
Based on the SPIRTPAT model, we establish the identity with the
impact factors of occupancy rate, unit area of revenue, temperature
factor, unit revenue of energy consumption. In this paper, we use the
LMDI (Logarithmic Mean Divisia Index) to decompose the impact
factors of energy consumption of hotel from Jan. to Dec. in 2001. The
results indicate that the occupancy rate and unit area of revenue are the
main factors that can increase unit area of energy consumption, and the
unit revenue of energy consumption is the main factor to restrain the
growth of unit area of energy consumption. When the energy
consumption of hotel can appear abnormal, the hotel manager can
carry out energy saving management and control according to the
contribution value of impact factors.
Abstract: Genetic algorithm is widely used in optimization
problems for its excellent global search capabilities and highly parallel
processing capabilities; but, it converges prematurely and has a poor
local optimization capability in actual operation. Simulated annealing
algorithm can avoid the search process falling into local optimum. A
hybrid genetic algorithm based on simulated annealing is designed by
combining the advantages of genetic algorithm and simulated
annealing algorithm. The numerical experiment represents the hybrid
genetic algorithm can be applied to solve the function optimization
problems efficiently.
Abstract: A sign pattern is a matrix whose entries belong to the set
{+,−, 0}. An n-by-n sign pattern A is said to allow an eventually
positive matrix if there exist some real matrices A with the same
sign pattern as A and a positive integer k0 such that Ak > 0 for all
k ≥ k0. It is well known that identifying and classifying the n-by-n
sign patterns that allow an eventually positive matrix are posed as two
open problems. In this article, the tree sign patterns of small order
that allow an eventually positive matrix are classified completely.
Abstract: To study the dynamic mechanics response of asphalt
pavement under the temperature load and vehicle loading, asphalt
pavement was regarded as multilayered elastic half-space system, and
theory analysis was conducted by regarding dynamic modulus of
asphalt mixture as the parameter. Firstly, based on the dynamic
modulus test of asphalt mixture, function relationship between the
dynamic modulus of representative asphalt mixture and temperature
was obtained. In addition, the analytical solution for thermal stress in
single layer was derived by using Laplace integral transformation and
Hankel integral transformation respectively by using thermal
equations of equilibrium. The analytical solution of calculation model
of thermal stress in asphalt pavement was derived by transfer matrix
of thermal stress in multilayer elastic system. Finally, the variation of
thermal stress in pavement structure was analyzed. The result shows
that there is obvious difference between the thermal stress based on
dynamic modulus and the solution based on static modulus. So the
dynamic change of parameter in asphalt mixture should be taken into
consideration when theoretical analysis is taken out.
Abstract: The research of juice flavor forecasting has become
more important in China. Due to the fast economic growth in China,
many different kinds of juices have been introduced to the market. If a
beverage company can understand their customers’ preference well,
the juice can be served more attractive. Thus, this study intends to
introducing the basic theory and computing process of grapes juice
flavor forecasting based on support vector regression (SVR). Applying
SVR, BPN, and LR to forecast the flavor of grapes juice in real data
shows that SVR is more suitable and effective at predicting
performance.
Abstract: The final energy use can be divided mainly in four sectors: commercial, industrial, residential, and transportation. The trend in final energy consumption by sector plays as a most straightforward way to provide a wide indication of progress for reducing energy consumption and associated environmental impacts by different end use sectors. The average share of end use energy for residential sector in the world was nearly 20% until 2011, in Germany a higher proportion is between 25% and 30%. However, it remains less studied than energy use in other three sectors as well its impacts on climate and environment. The reason for this involves a wide range of fields, including the diversity of residential construction like different housing building design and materials, living or energy using behavioral patterns, climatic condition and variation as well other social obstacles, market trend potential and financial support from government.
This paper presents an extensive and in-depth analysis of the manner by which projects researched and operated by authors in the fields of energy efficiency primarily from the perspectives of both technical potential and initiative energy saving consciousness in the residential sectors especially in social housing buildings.
Abstract: Among modern airflow measurement methods,
Particle Image Velocimetry (PIV) and Particle Tracking Velocimetry
(PTV), as visualized and non-instructive measurement techniques, are
playing more important role. This paper conducts a comparative
experimental study for airflow measurement employing both
techniques with the same condition. Velocity vector fields, velocity
contour fields, voticity profiles and turbulence profiles are selected as
the comparison indexes. The results show that the performance of both
PIV and PTV techniques for airflow measurement is satisfied, but
some differences between the both techniques are existed, it suggests
that selecting the measurement technique should be based on a
comprehensive consideration.
Abstract: According to the demand of the power and
refrigeration industry, the theoretical and practical teachings of the
Thermal Energy and Power Engineering characteristic specialty in
china are studied. The teaching reform and practice of the Thermal
Energy and Power Engineering specialty have been carried out,
including construction and reform measures, teaching reform and
practice, features, and achievements. Proved by practices, the
theoretical and practical teaching effects are obvious. The study results
can provides certain reference experience for theoretical and practical
teachings of the related specialties in china.
Abstract: It is usually difficult for students to understand some
basic theories in learning thermal energy and power engineering
course. A new teaching method was proposed that we should introduce
the comparison research method of those theories to help them being
understood. “Homogeneous and heterogeneous catalysis” teaching is
analyzed as an example by comparison research method.
Abstract: It was expected to benefit patient with hemiparesis after stroke by extensive arm rehabilitation, to partially regain forearm and hand function. This paper propose a robotic rehabilitation arm in assisting the hemiparetic patient to learn new ways of using and moving their weak arms. In this study, the robotic arm was driven by a somatosensory stimulated brain computer interface (BCI), which is a new modality BCI. The use of somatosensory stimulation is not only an input for BCI, but also a electrical stimulation for treatment of hemiparesis to strengthen the arm and improve its range of motion. A trial of this robotic rehabilitation arm was performed in a stroke patient with pure motor hemiparesis. The initial trial showed a promising result from the patient with great motivation and function improvement. It suggests that robotic rehabilitation arm driven by somatosensory BCI can enhance the rehabilitation performance and progress for hemiparetic patients after stroke.
Abstract: In this paper, a new CMOS current-mode single input and multi-outputs (SIMO) universal filter and quadrature oscillator with a similar circuit are proposed. The circuits only consist of three Current differencing transconductance amplifiers (CDTA) and two grounded capacitors, which are resistorless, and they are suitable for monolithic integration. The universal filter uses minimum CDTAs and passive elements to realize SIMO type low-pass (LP), high-pass (HP), band-pass (BP) band-stop (BS) and all-pass (AP) filter functions simultaneously without any component matching conditions. The angular frequency (ω0) and the quality factor (Q) of the proposed filter can be electronically controlled and tuned orthogonal. By some modifications of the filter, a new current-mode four-phase quadrature oscillator (QO) can be obtained easily. The condition of oscillation (CO) and frequency of oscillation (FO) of the QO can be controlled electronically and independently through the bias current of the CDTAs, and it is suitable for variable frequency oscillator. Moreover, all the passive and active sensitivities of the circuits are low. SPICE simulation results are included to confirm the theory.
Abstract: To improve the water quality of lakes and control algae blooms, the effects of Vallisneria asiatica which is one of aquatic plants spread over Lake Taihu, with different biomasses on the water quality and algae communities were researched. The results indicated that V. asiatica could control an excess of Microcystis spp. when the V. asiatica biomass was larger than 50g in the tank with 30L solution in the laboratory. Planktonic and epiphytic algae responded differently to V. asiatica. The presence of macrophyte V. asiatica in eutrophic waters has a positive effect on algae compositions because of different sensitivities of algae species to allelopathic substances released by macrophyte V. asiatica. That is, V. asiatica could inhibit the growth of Microcystis spp. effectively and was benefited to the diatom on the condition in the laboratory.
Abstract: Various cis-regulatory module (CRM) predictors have been proposed in the last decade. Several well-established CRM predictors adopted different categories of prediction strategies, including window clustering, probabilistic modeling and phylogenetic footprinting. Appropriate integration of them has a potential to achieve high quality CRM prediction. This study analyzed four existing CRM predictors (ClusterBuster, MSCAN, CisModule and MultiModule) to seek a predictor combination that delivers a higher accuracy than individual CRM predictors. 465 CRMs across 140 Drosophila melanogaster genes from the RED fly database were used to evaluate the integrated CRM predictor proposed in this study. The results show that four predictor combinations achieved superior performance than the best individual CRM predictor.
Abstract: As creative economy is important theme for national policy, many countries have been raising investments through national R&D programs. Since not all of programs are aligned with the ultimate vision and R&D investment is one of the most decisive elements, the strategic fit of national R&D programs should be evaluated for effective resource allocation. This study aims at identifying the factors of strategic fit of national R&D program on the creative economy policy. For this purpose, the balanced scorecard (BSC) model for R&D is utilized to translate national strategic objectives into a set of coherent performance factors.
Abstract: SUVA (equivalent to UV254/DOC) value in raw water is a precursor for the formation of trihalomethane during chlorination at a water treatment plant. This study collected rapidly filtered water from an advanced water treatment plant for use in experiments on raw water. The removal rate of treating the trihalomethanes formation potential (THMFP) was conducted by using a biological activated carbon. The hydraulic retention time and SUVA loading were major factors in biological degradation tests. The results showed that biological powder-activated carbon (BPAC) lowered the average concentration of UV254 and value of SUVA in raw water. A removal efficiency of THMFP was present in the treatment of the three primary organic carbon items. These results highlighted the importance of the BPAC had an excellent treatment efficiency on THMFP.
Abstract: An expressed sequence tag (EST) analysis provideus portions of expressed genes. We have constructed cDNA library and determined randomly sequences from cDNA library clones of T. molitor injected with acholeplasma lysate. We identified the homologous to a galectin gene. As the result of cloning and characterization of novel, we found that the protein has an open reading frame (ORF) of 495 bp, with 164 amino acid residues and molecular weight of 18.5 kDa. To characterize the role of novel Tm-galectin in immune system, we quantified the mRNA level of galectin at different times after treatment with immune elicitors. The galectin mRNA was up-regulated about 7-folds within 18 hrs. This suggests that Tm-galectin is a novel member of animal lectins, and has a role in the process of pathogen recognition. Our study would be helpful for the study on immune defense system and signaling cascade.