Abstract: Anthocyanins are natural pigments with effective UV
protection but their topical use could be limited due to their
physicochemical characteristics. An attempt to overcome such
limitations by complexation of 2 major anthocyanin-rich sources, C.
ternatea and Z. mays, has potentiated its use as topical antiinflammatory.
Cell studies indicate no cytotoxicity of the
anthocyanin complex (AC) up to 1 mg/ml tested in HaCaT and
human fore head fibroblasts by MTT. Croton oil-induced ear edema
in Wistar rats suggests an effective dose of 5 mg/cm2 of AC as a
topical anti-inflammatory in comparison to 0.5 mg/cm2 of
fluocinolone acetonide. Niosomal encapsulation of the AC
significantly prolonged the anti-inflammatory activity particularly at
8 h after topical application (p = 0.0001). The AC was not cytotoxic
and its anti-inflammatory and activity was dose-dependent and
prolonged by niosomal encapsulation. It has also shown to promote
collagen type 1 production in cell culture. Thus, AC could be a
potential candidate for topical anti-inflammatory agent from natural
resources.
Abstract: Liver disorders are one of the major problems of the
world. Despite its frequent occurrence, high morbidity and high
mortality, its medical management is currently inadequate. This study
was designed to evaluate the hepatoprotective effect of saponin
extract of the root of Garcinia kola on the integrity of the liver of
paracetamol induced wistar albino rats. Twenty five (25) male adult
wistar albino rats were divided into five (5) groups. Group I was the
Control group that received distilled water only, group II was the
negative control that received 2 g/kg of paracetamol on the 13th day,
and group III, IV and V were pre-treated with 100, 200 and
400mg/kg of the saponin extract before inducing the liver damage on
the 13th day with 2 g/kg of paracetamol. Twenty four (24) h after
administration, the rats were sacrificed and blood samples were
collected. The serum Alanine Transaminase (ALT), Aspartate
Transaminase (AST), Alkaline Phosphatase (ALP) activities,
Bilirubin and conjugated bilirubin, glucose and protein
concentrations were evaluated. The liver was fixed immediately in
Formalin and was processed and stained in Haematoxylin and Eosin
(H&E). Administration of saponin extract from the root of Garcinia
kola significantly decreased paracetamol induced elevated enzymes
in the test group. Also histological observations showed that saponin
extract of the root of Garcinia kola exhibited a significant liver
protection against the toxicant as evident by the cells trying to return
to normal. Saponin extract from the root of Garcinia kola indicated a
protection of structural integrity of the hepatocytic cell membrane
and regeneration of the damaged liver.
Abstract: Liposome plays an important role in medical and
pharmaceutical science as e.g. nano scale drug carriers. Liposomes
are vesicles of varying size consisting of a spherical lipid bilayer and
an aqueous inner compartment. Magnet-driven liposome used for the
targeted delivery of drugs to organs and tissues. These liposome
preparations contain encapsulated drug components and finely
dispersed magnetic particles.
Liposomes are vesicles of varying size consisting of a spherical
lipid bilayer and an aqueous inner compartment that are generated in
vitro. These are useful in terms of biocompatibility, biodegradability,
and low toxicity, and can control biodistribution by changing the size,
lipid composition, and physical characteristics. Furthermore,
liposomes can entrap both hydrophobic and hydrophilic drugs and are
able to continuously release the entrapped substrate, thus being useful
drug carriers. Magnetic liposomes (MLs) are phospholipid vesicles
that encapsulate magneticor paramagnetic nanoparticles. They are
applied as contrast agents for magnetic resonance imaging (MRI).
The biological synthesis of nanoparticles using plant extracts plays
an important role in the field of nanotechnology. Green-synthesized
magnetite nanoparticles-protein hybrid has been produced by treating
Iron (III) / Iron (II) chloride with the leaf extract of Datura inoxia.
The phytochemicals present in the leaf extracts act as a reducing as
well stabilizing agents preventing agglomeration, which include
flavonoids, phenolic compounds, cardiac glycosides, proteins and
sugars.
The magnetite nanoparticles-protein hybrid has been trapped
inside the aqueous core of the liposome prepared by reversed phase
evaporation (REV) method using oleic and linoleic acid which has
been shown to be driven under magnetic field confirming the
formation magnetic liposome (ML). Chemical characterization of
stealth magnetic liposome has been performed by breaking the
liposome and release of magnetic nanoparticles. The presence iron
has been confirmed by colour complex formation with KSCN and
UV-Vis study using spectrophotometer Cary 60, Agilent.
This magnet driven liposome using nanoparticles-protein hybrid
can be a smart vesicles for the targeted drug delivery.
Abstract: Medulloblastoma (MB) is one of the most prevalent
brain tumours among paediatrics. Although its management has
evolved over time still there is need to find new therapeutic targets
for MB that can result in less normal tissue toxicity while improving
survival and reducing recurrence. This literature review is aimed at
finding new potential therapeutic targets for MB focusing on viruses
that can be used as potential targets for MB. The review also gives an
over-view of management of paediatric Medulloblastoma focusing on
Radiotherapy management.
Abstract: The efficiency of wood vinegar mixed with each
individual of three plants extract such as: citronella grass
(Cymbopogon nardus), neem seed (Azadirachta indica A. Juss), and
yam bean seed (Pachyrhizus erosus Urb.) were tested against the
second instar larvae of housefly (Musca domestica L.). Steam
distillation was used for extraction of the citronella grass while neem
and yam bean were simple extracted by fermentation with ethyl
alcohol. Toxicity test was evaluated in laboratory based on two
methods of larvicidal bioassay: topical application method (contact
poison) and feeding method (stomach poison). Larval mortality was
observed daily and larval survivability was recorded until the
survived larvae developed to pupae and adults. The study resulted
that treatment of wood vinegar mixed with citronella grass showed
the highest larval mortality by topical application method (50.0%)
and by feeding method (80.0%). However, treatment of mixed wood
vinegar and neem seed showed the longest pupal duration to 25 day
and 32 days for topical application method and feeding method
respectively. Additional, larval duration on treated M. domestica
larvae was extended to 13 days for topical application method and 11
days for feeding method. Thus, the feeding method gave higher
efficiency compared with the topical application method.
Abstract: Pesticides and drugs used in agriculture and veterinary
medicine may end up in aquatic environments and bioaccumulate in
the food chain, thus causing serious problems for fauna and human
health. For determination of the toxic effects of atrazine herbicide on
Caspian kutum, Rutilus frisii kutum larvae, the 96-h LC50 of atrazine
was measured for newly hatched larvae as 18.53 ppm. Toxicity of
atrazine herbicide on Caspian kutum larvae was investigated using
concentrations: 9.25ppm, 4.62 ppm and 2.31 ppm for 7 days.
Comparison of the length, weight and condition factor showed that
no significant differences between atrazine exposed and control
groups. The concentration of Na+, K+, Ca2+, Mg2+ and Cl- in whole
body of larvae in control and atrazine exposure groups were
measured and the results showed that concentrations of all these ions
is higher in atrazine exposure group than control group. It is obvious
from this study that atrazine negatively affects osmoregulation
process and changes ion compositions of the body even at sublethal
concentration and acute exposure but have no effects on growth
parameters of the body.
Abstract: The presence of endocrine-disrupting compounds,
such as bisphenol A (BPA), in the environment can cause serious
health problems. However, there are controversial opinions. This
study investigated the reproductive, metabolic, oxidative and
immunologic-disrupting effects of bisphenol A in male rabbits.
Rabbits were divided into five groups. The first four rabbit groups
were administered oral BPA (1, 10, 50, or 100 mg/kg/day) for ten
weeks. The fifth group was administered corn oil as the vehicle. BPA
significantly decreased serum testosterone, estradiol and the free
androgen index (FAI) and significantly increased sex hormone
binding globulin (SHBG) compared with the placebo group. The
higher doses of BPA showed a significant decrease in follicular
stimulating hormone (FSH) and luteinizing hormone (LH). A
significant increase in blood glucose levels was identified in the BPA
groups. The non-significant difference in insulin levels is a novel
finding. The cumulative testicular toxicity of BPA was clearly
demonstrated by the dose-dependent decrease in absolute testes
weight, primary measures of semen quality and a significant increase
in testicular malonaldehyde (MDA). Moreover, BPA significantly
decreased total antioxidant capacity (TAC) and significantly
increased immunoglobulin G (IgG) at the highest concentration. Our
results suggest that BPA, especially at higher doses, is associated
with many adverse effects on metabolism, oxidative stress, immunity,
sperm quality and markers of androgenic action.
Abstract: Malathion (ML) is a well known pesticide commonly
used in many agricultural and non-agricultural processes. Its toxicity
has been attributed primarily to the accumulation of acetylcholine
(Ach) at nerve junctions, due to the inhibition of acetylcholinesterase
(AChE). The aim of the current research was to study the protective
effect of the melissa plant extract against reproductive impairment
induced by malathion in 32 male albino rats, and the biological
experiment was divided into four groups (8 in each) that given
malathion (27 mg/kg; 1/50 of the LD50 for an oral dose) and/or
Melissa officinalis (MO) extract (200mg/kg/day) by gavages
technique. The sperm counts, sperm motility, sperm morphology,
FSH, LH, and testosterone levels had been determined in testes
homogenate at the end of the experiment. It is worthy to report that,
rats treated with melissa extract did not show a significant difference
when compared with the control group, while rats given malathion
alone had significantly lower sperm count, sperm motility, and
significantly higher abnormal sperm numbers, than the untreated
control rats as well as having significantly lower serum FSH, LH, and
testosterone levels compared with the control group. Administrations
of melissa extract restore all mentioned histological parameters
towards the control group and the melissa extract had a strong
positive protective effect against malathion toxicity. Results the of
biological parameters were confirmed by the histological
examination of rat testes and indicated that, both control and melissa
groups showing normal seminiferous tubules, while malathion group
testicular tissues had necrosis, edema in the seminiferous tubules and
degeneration of spermatogonial cells lining the seminiferous tubules
with incomplete spermatogenesis. The use of melissa against
malathion improved the histological picture and showing normal
seminiferous tubules with complete spermatogenesis and almost there
was no histopathological changes could be noted.
Abstract: Pomegranate (Punica granatum L.) is an ancient fruit of great medical interest and rich source of antioxidants. Pesticides as dimethoate play a crucial role in the occurrence many diseases in plants, animal and human. Therefore the ability of Pomegranate (Punica granatum L.) to alleviate hepatotoxicity induced by organophosphate pesticide dimethoate was investigated. Albino male rats were divided randomly into 4 groups and kept at 7 animals per group in an environmentally controlled condition for 6 weeks. The first group was served as a control group (basal diet), the second group fed on basal diet supplemented with 5% freeze dried pomegranate seeds, the third group fed on 20 ppm dimethoate contaminated diet and the last group fed on dimethoate contaminated diet supplemented with 5% freeze dried pomegranate seeds. The results revealed that administration of dimethoate caused high significant increased in liver functions: alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) activities as well as lipid peroxide (malonaldhyde, MDA); on the other hand high significant decreased on glutathione (GSH), glutathione peroxidase (GPx), albumin and total protein were observed. However addition of 5% freeze dried pomegranate seeds significantly improved all previously mentioned parameters. These results indicate the dimethoate induced hepatotoxicity and highlight the protective effect of pomegranate seeds as a potential protective agent against dimethoate induced hepatotoxicity. This may be attributed to the powerful antioxidants (polyphenols, total phenols, and total flavonoids) which present in high levels in pomegranate as well as improving the immunity by activation of antioxidant enzymes GSH and GPx.
Abstract: Azadirachta excelsa or locally known as sentang are
frequently used as a traditional medicine by diabetes patients in
Malaysia. However, less attention has been given to their toxicity
effect. Thus, the study is an attempt to examine the protective effect
of A. excelsa on the pancreas and to determine possible toxicity
mediated by the extract. Diabetes was induced experimentally in rats
by high-fat-diet for 16 weeks followed by intraperitoneal injection of
streptozotocin at dosage of 35 mg/kg of body weight. Declination of
the fasting blood glucose level was observed after continuous
administration of A. excelsa for 14 days twice daily. This is due to the
refining structure of the pancreas. However, surprisingly, the plant
extract reduced the leukocytes, erythrocytes, hemoglobin, MCHC and
lymphocytes. In addition, the rat treated with the plant extract
exhibited increment in AST and eosinocytes level. Overall, the
finding shows that A. excelsa possesses antidiabetic activity by
improving the structure of pancreatic islet of Langerhans but
involved in ameliorating of hematology and biochemical parameters.
Abstract: Two new metal-based anticancer chemotherapeutic
agents, [(Ph2Sn)2(HGuO)2(phen)Cl2] 1 and [(Ph3Sn)(HGuO)(phen)]-
Cl.CH3OH.H2O 2, were designed, prepared and characterized by
analytical and spectral (IR, ESI-Mass, 1H, 13C and 119Sn NMR)
techniques. The proposed geometry of Sn(IV) in 1 and 2 is distorted
octahedral and distorted trigonal-bipyramidal, respectively. Both 1
and 2 exhibit potential cytotoxicity in vitro against MCF-7, HepG-2
and DU-145 cell lines. The intrinsic binding constant (Kb) values of 1
(2.33 × 105 M-1) and 2 (2.46 × 105 M-1) evaluated from UV-Visible
absorption studies suggest non-classical electrostatic mode of
interaction via phosphate backbone of DNA double helix. The Stern-
Volmer quenching constant (Ksv) of 1 (9.74 × 105 M-1) and 2 (2.9 ×
106 M-1) determined by fluorescence studies suggests the groove
binding and intercalation mode for 1 and 2, respectively. Effective
cleavage of pBR322 DNA is induced by 1.Their interaction with
DNA of cancer cells may account for potency.
Abstract: The protective effect of hesperidin was investigated in
rats exposed to liver injury induced by a single intraperitoneal
injection of cyclophosphamide (CYP) at a dose of 150 mg kg-1.
Hesperidin treatment (100 mg kg-1/day, orally) was applied for seven
days, starting five days before CYP administration. Hesperidin
significantly decreased the CYP-induced elevations of serum alanine
aminotransferase, and hepatic malondialdehyde and myeloperoxidase
activity, significantly prevented the depletion of hepatic glutathione
peroxidase activity resulted from CYP administration. Also,
hesperidin ameliorated the CYP-induced liver tissue injury observed
by histopathological examination. In addition, hesperidin decreased
the CYP-induced expression of inducible nitric oxide synthase, tumor
necrosis factor-α, cyclooxygenase-2, Fas ligand, and caspase-9 in
liver tissue. It was concluded that hesperidin may represent a
potential candidate to protect against CYP-induced hepatotoxicity.
Abstract: Portugal is an important wine and olive oil producer, activities which generate a high quantity of residues commonly called grape stalks and olive cake, respectively. In this work grape stalks and olive cake were used as lignocellulosic adsorbents for wastewater containing lead treatment. To attain a better knowledge of the factors that could influence the quality of the treated wastewater, a chemical characterization of the materials used in the treatment was done. To access the ecotoxicological safety of the treated wastewater, several tests were performed.
The results of the toxicity test show that the samples leachate has a mild effect on the living models tested. The tests performed in lemna and bacteria were the most sensible to toxicity effects of the samples. The results obtained in this work evidenced the importance of use of simple and fast toxicity tests to predict impacts in the environment.
Abstract: Bisphenol-A (BPA) is one of the highest volume chemicals produced worldwide in the plastic industry. This compound is mostly used in producing polycarbonate plastics that are often used for food and beverage storage, and BPA is also a component of epoxy resins that are used to line food and beverage containers. Studies performed in this area indicated that BPA could be extracted from such products while they are in contact with food. Therefore, BPA exposure is presumed. In this paper, the chemical structure of BPA, factors affecting BPA migration to food and beverages, effects on health, and recent regulations will be reviewed.
Abstract: The volatile organic compounds - BTEX (Benzene, Toluene, Ethylbenzene, and Xylene) petroleum derivatives, have high rates of toxicity, which may carry consequences for human health, biota and environment. In this directon, this paper proposes a method of treatment of these compounds by using corona discharge plasma technology. The efficiency of the method was tested by analyzing samples of BTEX after going through a plasma reactor by gas chromatography method. The results show that the optimal residence time of the sample in the reactor was 8 minutes.
Abstract: The production of aluminum alloys and ingots –
starting from the processing of alumina to aluminum, and the final
cast product – was studied using a Life Cycle Assessment (LCA)
approach. The studied aluminum supply chain consisted of a carbon
plant, a reduction plant, a casting plant, and a power plant. In the
LCA model, the environmental loads of the different plants for the
production of 1 ton of aluminum metal were investigated. The impact
of the aluminum production was assessed in eight impact categories.
The results showed that for all of the impact categories the power
plant had the highest impact only in the cases of Human Toxicity
Potential (HTP) the reduction plant had the highest impact and in the
Marine Aquatic Eco-Toxicity Potential (MAETP) the carbon plant
had the highest impact. Furthermore, the impact of the carbon plant
and the reduction plant combined was almost the same as the impact
of the power plant in the case of the Acidification Potential (AP). The
carbon plant had a positive impact on the environment when it come
to the Eutrophication Potential (EP) due to the production of clean
water in the process. The natural gas based power plant used in the
case study had 8.4 times less negative impact on the environment
when compared to the heavy fuel based power plant and 10.7 times
less negative impact when compared to the hard coal based power
plant.
Abstract: In this study the administration of 660 mg/kg of the ethanolic extract of the Alhagigraecorum (Camel Thorn)to mice, showed a significant decrease in the level of transaminases in animals treated with a combination of CTE plus carbon tetrachloride (CCl4) or acetaminophen as compared to animals receiving CCl4 or acetaminophen alone. Histopatological investigation also confirmed that, camel thorn extract protects liver against damage-induced either by carbon tetrachloride or acetaminophen. On the other hand the cardiac toxicity produced by adriamycine was significantly increased in the presence of the ethanolic extract of camel thorn. Our study suggested that camel thorn can protect the liver against the injury produced by carbon tetrachloride or acetaminophen, with unexpected increase in the cardiac toxicity –induced by adriamycin in rodents.
Abstract: Water a vital component for all living forms is derived from variety of sources, including surface water (rivers, lakes, reservoirs and ponds) and ground water (aquifers). Over the years of time, water bodies are subjected to human interference regularly resulting in deterioration of water quality. Therefore, pollution of water bodies has become matter of global concern. As the water quality closely relate to human health, water analysis before usage is of immense importance. Improper management of water bodies can cause serious problems in availability and quality of water. The quality of water may be described according to their physico-chemical and microbiological characteristics. For effective maintenance of water quality through appropriate control measures, continuous monitoring of metals, physico-chemical and biological parameter is essential for the establishment of baseline data for the water quality in any study area. The present study has focused on to explore the status of water pollution in various areas and to estimate the magnitude of its toxicity using different bioassay.
Abstract: Environmental and toxicological characteristics of formulated pesticides may substantially differ from those of their active ingredients or other components alone. This phenomenon is demonstrated in the case of the herbicide active ingredient glyphosate. Due to its extensive application, this active ingredient was found in surface and ground water samples collected in Békés County, Hungary, in the concentration range of 0.54–0.98 ng/ml. The occurrence of glyphosate appeared to be somewhat higher at areas under intensive agriculture, industrial activities and public road services, but the compound was detected at areas under organic (ecological) farming or natural grasslands, indicating environmental mobility. Increased toxicity of the formulated herbicide product Roundup compared to that of glyphosate was observed on the indicator aquatic organism Daphnia magna Straus. Acute LC50 values of Roundup and its formulating adjuvant polyethoxylated tallowamine (POEA) exceeded 20 and 3.1 mg/ml, respectively, while that of glyphosate (as isopropyl salt) was found to be substantially lower (690-900 mg/ml) showing good agreement with literature data. Cytotoxicity of Roundup, POEA and glyphosate has been determined on the neuroectodermal cell line, NE-4C measured both by cell viability test and holographic microscopy. Acute toxicity (LC50) of Roundup, POEA and glyphosate on NE-4C cells was found to be 0.013±0.002%, 0.017±0.009% and 6.46±2.25%, respectively (in equivalents of diluted Roundup solution), corresponding to 0.022±0.003 and 53.1±18.5 mg/ml for POEA and glyphosate, respectively, indicating no statistical difference between Roundup and POEA and 2.5 orders of magnitude difference between these and glyphosate. The same order of cellular toxicity seen in average cell area has been indicated under quantitative cell visualization. The results indicate that toxicity of the formulated herbicide is caused by the formulating agent, but in some parameters toxicological synergy occurs between POEA and glyphosate.
Abstract: A presentation of the design of the Organic Rankine cycle (ORC) with heat regeneration and superheating processes is a subject of this paper. The maximum temperature level in the ORC is considered to be 110°C and the maximum pressure varies up to 2.5MPa. The selection process of the appropriate working fluids, thermal design and calculation of the cycle and its components are described. With respect to the safety, toxicity, flammability, price and thermal cycle efficiency, the working fluid selected is R134a. As a particular example, the thermal design of the condenser used for the ORC engine with a theoretical thermal power of 179 kW was introduced. The minimal heat transfer area for a completed condensation was determined to be approximately 520m2.