Abstract: In this research, dried linden (Tilia argentea) leaves and blossoms were used as a raw material for mineral enriched herbal tea beverage production. For this aim, %1 dried linden was infused with boiling water (100 °C) for 5 minutes. After cooling, sucrose, citric acid, ascorbic acid, natural lemon flavor and natural mineral water were added. Beverage samples were plate filtered, filled into 200-mL glass bottles, capped then pasteurized at 98 °C for 15 minutes. Water soluble dry matter, titratable acidity, ascorbic acid, pH, minerals (Fe, Ca, Mg, K, Na), color (L*, a*, b*), turbidity, bioaccessible phenolics and antioxidant capacity were analyzed. Water soluble dry matter, titratable acidity, and ascorbic were determined as 7.66±0.28 g/100 g, 0.13±0.00 g/100 mL, and 19.42±0.62 mg/100 mL, respectively. pH was measured as 3.69. Fe, Ca, Mg, K and Na contents of the beverage were determined as 0.12±0.00, 115.48±0.05, 34.72±0.14, 48.67±0.43 and 85.72±1.01 mg/L, respectively. Color was measured as 13.63±0.05, -4.33±0.05, and 3.06±0.05 for L*, a*, and b* values. Turbidity was determined as 0.69±0.07 NTU. Bioaccessible phenolics were determined as 312.82±5.91 mg GAE/100 mL. Antioxidant capacities of chemical (MetOH:H2O:HCl) and physiological extracts (in vitro digestive enzymatic extraction) with DPPH (27.59±0.53 and 0.17±0.02 μmol trolox/mL), FRAP (21.01±0.97 and 13.27±0.19 μmol trolox/mL) and CUPRAC (44.71±9.42 and 2.80±0.64 μmol trolox/mL) methods were also evaluated. As a result, enrichment with natural mineral water was proposed for the development of functional and nutritional values together with a good potential for commercialization.
Abstract: A number of toxic chlorophenolic compounds are formed during pulp bleaching. The nature and concentration of these chlorophenolic compounds largely depends upon the amount and nature of bleaching chemicals used. These compounds are highly recalcitrant and difficult to remove but are partially removed by the biochemical treatment processes adopted by the paper industry. Identification and estimation of these chlorophenolic compounds has been carried out in the primary and secondary clarified effluents from the paper mill by GCMS. Twenty-six chorophenolic compounds have been identified and estimated in paper mill waste waters. Electrochemical treatment is an efficient method for oxidation of pollutants and has successfully been used to treat textile and oil waste water. Electrochemical treatment using less expensive anode material, stainless steel electrodes has been tried to study their removal. The electrochemical assembly comprised a DC power supply, a magnetic stirrer and stainless steel (316 L) electrode. The optimization of operating conditions has been carried out and treatment has been performed under optimized treatment conditions. Results indicate that 68.7% and 83.8% of cholorphenolic compounds are removed during 2 h of electrochemical treatment from primary and secondary clarified effluent respectively. Further, there is a reduction of 65.1, 60 and 92.6% of COD, AOX and color, respectively for primary clarified and 83.8%, 75.9% and 96.8% of COD, AOX and color, respectively for secondary clarified effluent. EC treatment has also been found to increase significantly the biodegradability index of wastewater because of conversion of non- biodegradable fraction into biodegradable fraction. Thus, electrochemical treatment is an efficient method for the degradation of cholorophenolic compounds, removal of color, AOX and other recalcitrant organic matter present in paper mill waste water.
Abstract: Catalytic wet air oxidation (CWAO) is normally carried out at elevated temperature and pressure. This work investigates the potential of NiO-CeO2 nano-catalyst in CWAO of paper industry wastewater under milder operating conditions of 90 °C and 1 atm. The NiO-CeO2 nano-catalysts were synthesized by a simple co-precipitation method and characterized by X-ray diffraction (XRD), before and after use, in order to study any crystallographic change during experiment. The extent of metal-leaching from the catalyst was determined using the inductively coupled plasma optical emission spectrometry (ICP-OES). The catalytic activity of nano-catalysts was studied in terms of total organic carbon (TOC), adsorbable organic halides (AOX) and chlorophenolics (CHPs) removal. Interestingly, mixed oxide catalysts exhibited higher activity than the corresponding single-metal oxides. The maximum removal efficiency was achieved with Ce40Ni60 catalyst. The results indicate that the CWAO process is efficient in removing the priority organic pollutants from wastewater, as it exhibited up to 59% TOC, 55% AOX, and 54 % CHPs removal.
Abstract: Aqueous ethanol and aqueous acetone extracts of
Moringa oleifera (outer pericarp of immature fruit and flower) and
Sesbania grandiflora white variety (flower and leaf) were examined
for radical scavenging capacities and antioxidant activities. Ethanol
extract of S. grandiflora (flower and leaf) and acetone extract of M.
oleifera (outer pericarp of immature fruit and flower) contained
relatively higher levels of total dietary phenolics than the other
extracts. The antioxidant potential of the extracts were assessed by
employing different in vitro assays such as reducing power assay,
DPPH˙, ABTS˙+ and ˙OH radical scavenging capacities,
antihemolytic assay by hydrogen peroxide induced method and metal
chelating ability. Though all the extracts exhibited dose dependent
reducing power activity, acetone extract of all the samples were
found to have more hydrogen donating ability in DPPH˙ (2.3% -
65.03%) and hydroxyl radical scavenging systems (21.6% - 77.4%)
than the ethanol extracts. The potential of multiple antioxidant
activity was evident as it possessed antihemolytic activity (43.2 % to
68.0 %) and metal ion chelating potency (45.16 - 104.26 mg EDTA/g
sample). The result indicate that acetone extract of M. oleifera (OPIF
and flower) and S. grandiflora (flower and leaf) endowed with
polyphenols, could be utilized as natural antioxidants/nutraceuticals.
Abstract: In the past few years, high consumption of soft drinks
has attracted negative attention world-wide due to its possible
adverse effects, leading the health conscious people to find
alternative nutraceutical or herbal health drinks. In the present study,
a nutraceutical soft drink was developed utilizing some easily
available and well known traditional herbs having nutritional
potential. The key ingredients were selected as bael, amla, lemon
juice, ashwagandha and poppy seeds based on their household routine
use in the summer with proven refreshing, cooling and energetic
feeling since ages. After several trials made, the final composition of
nutraceutical summer soft drink was selected as most suitable
combination based on the taste, physicochemical, microbial and
organoleptic point of view. The physicochemical analysis of the
prepared drink found to contain optimum level of titratable acidity,
total soluble solids and pH which were in accordance of the
commercial recommendations. There were no bacterial colonies
found in the product therefore found within limits. During the nine
point’s hedonic scale sensory evaluation, the drink was strongly liked
for colour, taste, flavour and texture. The formulation was found to
contain flavonoids (80mg/100ml), phenolics (103mg/100ml), vitamin
C (250mg/100ml) and has antioxidant potential (75.52%) apart from
providing several other essential vitamins, minerals and healthy
components. The developed nutraceutical drink provides an
economical and feasible option for the consumers with very good
taste combined with potential health benefits. The present drink is
potentially capable to replace the synthetic soft drinks available in the
market.
Abstract: Plants can contain a wide variety of substances with
antioxidative properties which are associated with important health
benefits. These positive health effects are of great importance at a
time when the environment is laden with many toxic substances. Five
selected herbal plants namely, Mimosa pudica, Phyllanthus niruri,
Ceiba pentandra, Eleusine polydactyla and Trema amboinensi, were
chosen for the experiment to investigate their total phenolics content
and antioxidant activities using ABTS radical cation decolorization
power, and ferric reducing antioxidant power. The total phenolic
content of each herbal plants ranges from 0.84 to 42.59 mg gallic acid
equivalent/g. The antioxidant activity in the ABTS radical cation
decolorization power varies from 0.005 to 0.362 mg trolox
equivalent/g and the FRAP ranges from 0.30 to 28.42 mg gallic acid
equivalent/g. Among the five medicinal plants, Mimosa pudica has
been an excellent performer in terms of the 3 parameters measured; it
is followed by Phyllanthus niruri. The 5 herbal plants do not have
equivalent antioxidant power. The relative high values for M. pudica
and P. niruri supports the medicinal value of both plants. The total
phenolics, ABTS and FRAP correlate strongly with one another.
Abstract: In order to detect and quantify the phenolic contents
of a wastewater with biosensors, two working electrodes based on
modified Poly(Pyrrole) films were fabricated. Enzyme horseradish
peroxidase was used as biomolecule of the prepared electrodes.
Various phenolics were tested at the biosensor. Phenol detection was
realized by electrochemical reduction of quinones produced by
enzymatic activity. Analytical parameters were calculated and the
results were compared with each other.
Abstract: Mech-Degla, Degla-Beida and Frezza are the date
(Phoenix dactylifera L.) common varieties with a more or less good
availability and feeble trade value. Some morphologic and
physicochemical factors were determined. Results show that the
whole date weight is significantly different (P= 95%) concerning
Mech-Degla and Degla-Beida which are more commercialized than
Frezza whereas the pulp mass proportion in relation to whole fruits is
highest for Frezza (88.28%). Moreover, there is a large variability
concerning the weights and densities of constitutive tissues in each
variety. The white tissue is dominant in Mech-Degla in opposite to
the two other varieties. The variance analyze showed that the
difference in weights between brown and white tissues is significant
(P = 95%) for all studied varieties. Some other morphologic and
chemical proprieties of the whole pulps and their two constitutive
parts (brown or pigmented and white) are also investigated. The
predominance of phenolics in Mech-Degla (4.01g/100g, w.b) and
Frezza (4.96 g/100g, w.b) pulps brown part is the main result
revealed in this study.
Abstract: The objective of this study was to optimize the extraction conditions for phenolic compounds, total flavonoids, and antioxidant activity from Deglet-Nour variety. The extraction of active components from natural sources depends on different factors. The knowledge of the effects of different extraction parameters is useful for the optimization of the process, as well for the ability to predict the extraction yield. The effects of extraction variables, namely types of solvent (methanol, ethanol and acetone) and extraction time (1h, 6h, 12h and 24h) on phenolics extraction yield were evaluated. It has been shown that the time of extraction and types of solvent have a statistically significant influence on the extraction of phenolic compounds from Deglet-Nour variety. The optimised conditions yielded values of 80.19 ± 6.37 mg GAE/100 g FW for TPC, 2.34 ± 0.27 mg QE/100 g FW for TFC and 90.20 ± 1.29% for antioxidant activity were methanol solvent and 6 hours of time. According to the results obtained in this study, Deglet-Nour variety can be considered as a natural source of phenolic compounds with good antioxidant capacity.
Abstract: Indian subcontinent has a plethora of traditional
medicine systems that provide promising solutions to lifestyle
disorders in an 'all natural way'. Spices and oilseeds hold
prominence in Indian cuisine hence the focus of the current study
was to evaluate the bioactive molecules from Linum usitatissinum
(LU), Lepidium sativum (LS), Nigella sativa (NS) and Guizotia
abyssinica (GA) seeds. The seeds were characterized for functional
lipids like omega-3 fatty acid, antioxidant capacity, phenolic
compounds, dietary fiber and anti-nutritional factors. Analysis of the
seeds revealed LU and LS to be a rich source of α-linolenic acid
(41.85 ± 0.33%, 26.71 ± 0.63%), an omega 3 fatty acid (using
GCMS). While studying antioxidant potential NS seeds demonstrated
highest antioxidant ability (61.68 ± 0.21 TEAC/ 100 gm DW) due to
the presence of phenolics and terpenes as assayed by the Mass
spectral analysis. When screened for anti-nutritional factor
cyanogenic glycoside, LS seeds showed content as high as 1674 ± 54
mg HCN / kg. GA is a probable good source of a stable vegetable oil
(SFA: PUFA 1:2.3). The seeds showed diversified bioactive profile
and hence further studies to use different bio molecules in tandem for
the development of a possible 'nutraceutical cocktail' have been
initiated..
Abstract: Polyphenolics and sugar are the components of many
fruit juices. In this work, the performance of ultra-filtration (UF) for
separating phenolic compounds from apple juice was studied by
performing batch experiments in a membrane module with an area of
0.1 m2 and fitted with a regenerated cellulose membrane of 1 kDa
MWCO. The effects of various operating conditions: transmembrane
pressure (3, 4, 5 bar), temperature (30, 35, 40 ºC), pH (2, 3, 4, 5),
feed concentration (3, 5, 7, 10, 15 ºBrix for apple juice) and feed flow
rate (1, 1.5, 1.8 L/min) on the performance were determined.
The optimum operating conditions were: transmembrane pressure
4 bar, temperature 30 ºC, feed flow rate 1 – 1.8 L/min, pH 3 and 10
Brix (apple juice). After performing ultrafiltration under these
conditions, the concentration of polyphenolics in retentate was
increased by a factor of up to 2.7 with up to 70% recovered in the
permeate and with approx. 20% of the sugar in that stream..
Application of diafiltration (addition of water to the concentrate) can
regain the flux by a factor of 1.5, which has been decreased due to
fouling. The material balance performed on the process has shown
the amount of deposits on the membrane and the extent of fouling in
the system. In conclusion, ultrafiltration has been demonstrated as a
potential technology to separate the polyphenolics and sugars from
their mixtures and can be applied to remove sugars from fruit juice.
Abstract: Yogurt is a coagulated milk product obtained from
the lactic acid fermentation by the action of Lactobacillus
bulgaricus and Streptococcus thermophilus. The additions of fruits
into milk may enhance the taste and the therapeutical values of milk
products. However fruits also may change the fermentation
behaviour. In this present study, the changes in physicochemical, the
peptide concentration, total phenolics content and the antioxidant
potential of yogurt upon the addition of Hylocereus polyrhizus and
Hylocereus undatus (white and red dragon fruit) were investigated.
Fruits enriched yogurt (10%, 20%, 30% w/w) were prepared and the
pH, TTA, syneresis measurement, peptide concentration, total
phenolics content and DPPH antioxidant inhibition percentage were
determined. Milk fermentation rate was enhanced in red dragon fruit
yogurt for all doses (-0.3606 - -0.4126 pH/h) while only white
dragon fruit yogurt with 20% and 30% (w/w) composition showed
increment in fermentation rate (-0.3471 - -0.3609 pH/h) compared to
plain yogurt (-0.3369pH/h). All dragon fruit enriched yogurts
generally showed lower pH readings (pH 3.95 - 4.03) compared to
plain yogurt (pH 4.05). Both fruit yogurts showed a higher lactic
acid percentage (1.14-1.23%) compared to plain yogurt (1.08%).
Significantly higher syneresis percentage (57.19 - 70.32%)
compared to plain yogurt (52.93%) were seen in all fruit enriched
yogurts. The antioxidant activity of plain yogurt (19.16%) was
enhanced by the presence of white and red dragon fruit (24.97-
45.74%). All fruit enriched yogurt showed an increment in total
phenolic content (36.44 - 64.43mg/ml) compared to plain yogurt
(20.25mg/ml). However, the addition of white and red dragon fruit
did not enhance the proteolysis of milk during fermentation.
Therefore, it could be concluded that the addition of white and red
dragon fruit into yogurt enhanced the milk fermentation rate, lactic
acid content, syneresis percentage, antioxidant activity, and total
phenolics content in yogurt.
Abstract: There is a growing interest in the food industry and in preventive health care for the development and evaluation of natural antioxidants from medicinal plant materials. In the present work, extracts of three medicinal plants (Tilia argentea, Crataegi folium leaves and Polygonum bistorta roots) used in Turkish phytotheraphy were screened for their phenolic profiles and antioxidant properties. Crude extracts were obtained from different parts of plants, by solidliquid extraction with pure water, 70% acetone and 70% methanol aqueous solvents. The antioxidant activity of the extracts was determined by ABTS.+ radical cation scavenging activity. The Folin Ciocalteu procedure was used to assess the total phenolic concentrations of the extracts as gallic acid equivalents. A modified liquid chromatography-electro spray ionization-mass spectrometry (LC-ESI-MS) was used to obtain chromatographic profiles of the phenolic compounds in the medicinal plants. The predominant phenolic compounds detected in different extracts of the plants were catechin, protocatechuic and chlorogenic acids. The highest phenolic contents were obtained by using 70% acetone as aqueous solvent, whereas the lowest phenolic contents were obtained by water extraction due to Folin Ciocalteu results. The results indicate that acetone extracts of Tilia argentea had the highest antioxidant capacity as free ABTS radical scavengers. The lowest phenolic contents and antioxidant capacities were obtained from Polygonum bistorta root extracts.
Abstract: Photoselective plastic films with thermic properties
are now available so that greenhouses clad with such plastics exhibit
a higher degree of “Greenhouse Effect” with a consequent increase in
night time temperature. In this study, we investigate the potential
benefits of a range of thermic plastic films used as greenhouse cover
materials on the vegetative and reproductive growth and development
of Iceberg lettuce (Lactuca sativa L). Transplants were grown under
thermic films and destructively harvested 4, 5, and 6 weeks after
transplanting. Thermic films can increase night temperatures up to 2
⁰C reducing the wide fluctuation in greenhouse temperature during
winter compared to the standard commercial film and consequently
increased the yield (leaf number, fresh weight, and dry weight) of
lettuce plants. Lettuce plants grown under Clear film respond to cold
stress by the accumulation of secondary products (phenolics, and
flavonoids).
Abstract: Sweet cherries (Prunus avium L.) contain various
phenolic compounds which contribute to total antioxidant activity.
Total polyphenols, tannins, flavonoids and anthocyanins, and
antioxidant capacity in a fruits of a number of selected sweet cherry
genotypes were investigated. Total polyphenols content ranged from
4.12 to 8.34 mg gallic acid equivantents/g dry fruit weight and total
tannins content ranged from 0.19 to 1.95 mg gallic acid equivalent/g
dry fruit weight. Total flavonoids were within the range 0.42-1.56 mg
of rutin equivalents/g dry fruit weight and total anthocyanins content
were between 0.35 and 0.69 mg cyanidin 3-glucoside equivalent/ g
dry fruit weight. Although sweet cherry fruits are a significant source
of different phenolic compounds, antioxidant activity of sweet
cherries is not related only with the total polyphenolics, flavonoids or
anthocyanins.
Abstract: Wood pyrolysis for Casuarina glauca, Casuarina cunninghamiana, Eucalyptus camaldulensis, Eucalyptus microtheca was made at 450°C with 2.5°C/min. in a flowing N2-atmosphere. The Eucalyptus genus wood gave higher values of specific gravity, ash , total extractives, lignin, N2-liquid trap distillate (NLTD) and water trap distillate (WSP) than those for Casuarina genus. The GHC of NLTD was higher for Casuarina genus than that for Eucalyptus genus with the highest value for Casuarina cunninghamiana. Guiacol, 4-ethyl-2-methoxyphenol and syringol were observed in the NLTD of all the four wood species reflecting their parent hardwood lignin origin. Eucalyptus camaldulensis wood had the highest lignin content (28.89%) and was pyrolyzed to the highest values of phenolics (73.01%), guaiacol (11.2%) and syringol (32.28%) contents in methylene chloride fraction (MCF) of NLTD. Accordingly, recoveries of syringol and guaiacol may become economically attractive from Eucalyptus camaldulensis.
Abstract: Supercritical carbon dioxide (SC-CO2) was used as a
solvent to extract oil from wheat bran. Extractions were carried out in a
semi-batch process at temperatures ranging from 40 to 60ºC and
pressures ranging from 10 to 30 MPa, with a carbon dioxide (CO2)
flow rate of 26.81 g/min. The oil obtained from wheat bran at different
extraction conditions was quantitatively measured to investigate the
solubility of oil in SC-CO2. The solubility of wheat bran oil was found
to be enhanced in high temperature and pressure. The composition of
fatty acids in wheat bran oil was measured by gas chromatography
(GC). Linoleic, palmitic, oleic and γ-linolenic acid were the major
fatty acids of wheat bran oil. Tocopherol contents in oil were analyzed
by high performance liquid chromatography (HPLC). The highest
amount of phenolics and tocopherols (α and β) were found at
temperature of 60ºC and pressure of 30 MPa.