Modified Poly(pyrrole) Film Based Biosensors for Phenol Detection

In order to detect and quantify the phenolic contents
of a wastewater with biosensors, two working electrodes based on
modified Poly(Pyrrole) films were fabricated. Enzyme horseradish
peroxidase was used as biomolecule of the prepared electrodes.
Various phenolics were tested at the biosensor. Phenol detection was
realized by electrochemical reduction of quinones produced by
enzymatic activity. Analytical parameters were calculated and the
results were compared with each other.





References:
[1] T. Ruzgas, E. Csöregi, J. Emneus, L. Gorton, G. Makro-Varga,
“Peroxidase-modified electrodes: Fundamentals and application,” Anal.
Chim. Acta, vol. 330, pp. 123-138, September 1996.
[2] J. Yu, S. Liu, H. Ju, “Mediator-free phenol sensor based on titania solgel
encapsulation matrix for immobilization of tyrosinase by a vapor
deposition method,” Biosens. Bioelectron., vol. 19, pp. 509-514,
December 2003.
[3] C. Capannesi, I. Ilaria Palchetti, M. Macsini, A. Parenti,
“Electrochemical sensor and biosensor for polyphenols detection in
olive oils,” Food Chemistry, vol. 71, pp. 553-562, December 2000.
[4] J. Metzger, M. Reiss, W. Hartmeier, “Amperometric phenol biosensor
based on a thermostable phenol hydroxylase,” Biosens. Bioelectron., vol.
13, pp. 1077-1082, November 1998.
[5] S. Rodriguez-Mozaz, M. J. Lopez de Alda, M. P. Marco, D. Barcelo,
“Biosensors for environmental monitoring,” Talanta, vol. 65, pp. 291-
297, January 2005.
[6] Y. L. Zhaou, R. H. Tian, J. F. Zhi, “Amperometric biosensor based on
tyrosinase immoblized on a boron-doped diamond electrode,” Biosens.
Bioelectron., vol. 22, pp. 822-828, January 2007.
[7] S. C. Chang, K. Rawson, C. J. McNeil, “Disposable tyrosinaseperoxidase
bi-enzyme sensor for amperometric detection of phenols,”
Biosens. Bioelectron., vol. 17, pp. 1015-1023, December 2002.
[8] A. Lindgren, J. Emn´eus, T. Ruzgas, L. Gorton, G. Marko- Varga,
“Amperometric detection of phenols using peroxidase-modified graphite
electrodes,” Anal. Chim. Acta, vol. 347, pp. 51-62, July 1997.
[9] Y. C. Tsai, C. Cheng-Chiu, “Amperometric biosensors based on
multiwalled carbon nanotube-Nafion-tyrosinase nanobiocomposites for
the determination of phenolic compounds,” Sens. Actuators B: Chem.,
vol. 125, pp. 10-16, July 2007.
[10] Q. Fan, D. Shan, H. Xue, Y. He, S. Cosnier, “Amperometric phenol
biosensor based on laponite-clay chitosan nanocomposite matrix,”
Biosens. Bioelectron., vol. 22, pp. 816-821, January 2007.
[11] Rajesh, K. Kaneto, “A new tyrosinase biosensor based on covalent
immobilization of enzyme on N-(3-aminopropyl) pyrrole polymer film,”
Curr. Appl. Phys., vol. 5, pp. 178-183, February 2005.
[12] S. Korkut Ozoner, M. Yalvac Can, B. Keskinler, E. Erhan, “A novel
Poly(gluteraldehyde-co-pyrrole)/horseradish peroxidase composite film
electrode,” Anal. Lett., vol. 42, pp. 3058-3072, November 2009.
[13] S. Korkut Ozoner, “Poly(glycidyl methacrylate-co-3-thienylmethyl
methacrylate based working electrodes for hydrogenperoxide
biosensing,” J Chem. Technol. Biotechnol., vol. 87, pp. 146-152,
January 2012.