Antioxidant Capacity and Total Phenolic Content of Aqueous Acetone and Ethanol Extract of Edible Parts of Moringa oleifera and Sesbania grandiflora
Aqueous ethanol and aqueous acetone extracts of
Moringa oleifera (outer pericarp of immature fruit and flower) and
Sesbania grandiflora white variety (flower and leaf) were examined
for radical scavenging capacities and antioxidant activities. Ethanol
extract of S. grandiflora (flower and leaf) and acetone extract of M.
oleifera (outer pericarp of immature fruit and flower) contained
relatively higher levels of total dietary phenolics than the other
extracts. The antioxidant potential of the extracts were assessed by
employing different in vitro assays such as reducing power assay,
DPPH˙, ABTS˙+ and ˙OH radical scavenging capacities,
antihemolytic assay by hydrogen peroxide induced method and metal
chelating ability. Though all the extracts exhibited dose dependent
reducing power activity, acetone extract of all the samples were
found to have more hydrogen donating ability in DPPH˙ (2.3% -
65.03%) and hydroxyl radical scavenging systems (21.6% - 77.4%)
than the ethanol extracts. The potential of multiple antioxidant
activity was evident as it possessed antihemolytic activity (43.2 % to
68.0 %) and metal ion chelating potency (45.16 - 104.26 mg EDTA/g
sample). The result indicate that acetone extract of M. oleifera (OPIF
and flower) and S. grandiflora (flower and leaf) endowed with
polyphenols, could be utilized as natural antioxidants/nutraceuticals.
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Carum nigrum (seed) essential oil, oleoresin, and their selected
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activity of ethanol extract of S. grandiflora (Agati Sesban) against
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of Sesbania grandiflora for anti urolithiatic and antioxidant properties,”
Nat. Med. vol. 62, pp. 300-307, 2008.
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nutritional, therapeutic, and prophylactic properties: Part 1,” Trees Life
J., vol. 1, pp. 5–19, 2005.
[16] S. Sreelatha, and P. R. Padma, “Antioxidant Activity and Total Phenolic
Content of Moringa oleifera Leaves in Two Stages of Maturity,” Plant
Foods for Human Nutr., vol. 64, pp. 303- 311, 2009.
[17] Q. E. Muhl, E. S. D. Toit, P. J. Robbertse, “Moringa oleifera
(horseradish tree) leaf adaptation to temperature regimes,” Int. J. Agric.
Biol., vol. 13, pp. 1021–1024, 2011.
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food plant with multiple medicinal uses,” Phytother. Res., vol. 21, pp.
17-25, 2007.
[19] P. Anjula, K. Pradheep, G. Rita, N. E. Roshini, D. C, Bhandari,
“‘Drumstick tree’ (Moringa oleifera Lam.): a multipurpose potential
species in India,” Genet. Resour. Crop Evol., vol. 58, pp. 453–460,
2011.
[20] R. Bennett, F. Mellon, J. Pratt, M. Dupont, L. Pernins, and P. Kroon,
“Profiling glucosinolates and phenolics in vegetative and reproductive
tissues of multi-purpose trees Moringa oleifera L. (horseradish tree) and
Moringa stenopetal L,” J. Agric. Food Chem., vol. 51, pp. 3546-5553,
2003.
[21] P. Siddhuraju, and K. Becker, “Antioxidant properties of various solvent
extracts of total phenolic constituents from three different agroclimatic
origins of drumstick tree (Moringa oleifera Lam),” J. Agric. Food
Chem., vol. 15, pp. 2144-2155, 2003.
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Upadhyay, and H. B. Singh, “Oxidative DNA damage protective
activity, antioxidant and anti-quorum sensing potentials of Moringa
oleifera,” Food Chem. Toxicol., vol. 47, pp. 1109–1116, 2009.
[23] M. K. Roy, M. Takenaka, S. Isobe, and T. Tsushida, “Antioxidant
potential, anti-proliferative activities, and phenolic content in watersoluble
fractions of some commonly consumed vegetables: Effects of
thermal treatment,” Food Chem., vol. 103, pp. 106–114, 2007.
[24] K. Shankar, M. M. Gupta, S. K. Srivastava, D. U. Bawankule, A. Pal,
and S. P. S. Khanuja, “Determination of bioactive nitrile glycoside(s) in
drumstick (Moringa oleifera) by reverse phase HPLC,” Food Chem.,
vol. 105, pp. 376–382, 2007.
[25] D. Yammuenart, W. Chavasiri, K. Pongrapeeporn, “Chemical
constituents of Moringa oleifera Lam,” Sci. Forum, vol. 3, pp. 80 – 81,
2008.
[26] N. K. Amaglo, R. N. Bennett, L. R. B. Curto, E. A. S. Rosa, V. L. Turco,
A. Giuffrida, L. A. Curto, F. Crea, and G. M. Timpo, “Profiling selected
phytochemicals and nutrients in different tissues of the multipurpose tree
Moringa oleifera L., grown in Ghana,” Food Chem., vol. 122, pp. 1047–
1054, 2010.
[27] T. T. Kivevele, M. M. Mbarawa, A. Bereczky, and Z. Mate, “Evaluation
of the Oxidation Stability of Biodiesel Produced from Moringa oleifera
Oil,” Energ. Fuel, vol. 25, pp. 5416– 5421, 2011.
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Dalhammar, “A simple purification and activity assay of the coagulant
protein from Moringa oleifera seed,” Water Res., vol. 39, pp. 2338–44,
2005.
[29] K. Prabhu, K. Murugan, A. Nareshkumar, N. Ramasubramanian, and S.
Bragadeeswaran, “Larvicidal and repellent potential of Moringa oleifera
against malarial vector, Anopheles stephensi Liston (Insecta: Diptera:
Culicidae). Asian Pacific J. Trop. Biomed., pp. 124-129, 2011.
[30] H. A. Jerri, K. J. Adolfsen, L. R. McCullough, D. Velegol, and S. B.
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@article{"International Journal of Biological, Life and Agricultural Sciences:72533", author = "Perumal Siddhuraju and Arumugam Abirami and Gunasekaran Nagarani and Marimuthu Sangeethapriya", title = "Antioxidant Capacity and Total Phenolic Content of Aqueous Acetone and Ethanol Extract of Edible Parts of Moringa oleifera and Sesbania grandiflora", abstract = "Aqueous ethanol and aqueous acetone extracts of
Moringa oleifera (outer pericarp of immature fruit and flower) and
Sesbania grandiflora white variety (flower and leaf) were examined
for radical scavenging capacities and antioxidant activities. Ethanol
extract of S. grandiflora (flower and leaf) and acetone extract of M.
oleifera (outer pericarp of immature fruit and flower) contained
relatively higher levels of total dietary phenolics than the other
extracts. The antioxidant potential of the extracts were assessed by
employing different in vitro assays such as reducing power assay,
DPPH˙, ABTS˙+ and ˙OH radical scavenging capacities,
antihemolytic assay by hydrogen peroxide induced method and metal
chelating ability. Though all the extracts exhibited dose dependent
reducing power activity, acetone extract of all the samples were
found to have more hydrogen donating ability in DPPH˙ (2.3% -
65.03%) and hydroxyl radical scavenging systems (21.6% - 77.4%)
than the ethanol extracts. The potential of multiple antioxidant
activity was evident as it possessed antihemolytic activity (43.2 % to
68.0 %) and metal ion chelating potency (45.16 - 104.26 mg EDTA/g
sample). The result indicate that acetone extract of M. oleifera (OPIF
and flower) and S. grandiflora (flower and leaf) endowed with
polyphenols, could be utilized as natural antioxidants/nutraceuticals.", keywords = "Antioxidant activity, Moringa oleifera,
Polyphenolics, Sesbania grandiflora, Underutilized vegetables.", volume = "8", number = "9", pages = "1090-9", }
