Abstract: Biogas, a clean renewable energy, is attracting a growing
concern of researchers and professionals in many fields. Based
on the natural and climatic conditions in semi-arid regions of northwestern
China, the present study introduces a specifically-designed
family-size biogas plant (with a digester of 10m3) with manure
and urine of animals and humanity as raw materials. The biogas
plant is applicable to areas with altitudes of more than 2000 meters
in northwestern China. In addition to the installation cost, a little
operational expenditure, structure, characteristics, benefits of this
small-scale biogas plant, this article introduces a wide range of
specific popularization methods such as training, financial support,
guided tour to the biogas plant, community-based group study and
delivery of operational manuals. The feasibility of the biogas plant is
explored on the basis of the availability of the raw materials. Simple
operations contained in the current work increase the possibility of
the wide use of this small-scale biogas plant in similar regions of the
world.
Abstract: As part of national epidemiological survey on bovine
viral diarrhea virus (BVDV), a total of 274 dejecta samples were
collected from 14 cattle farms in 8 areas of Xinjiang Uygur
Autonomous Region in northwestern China. Total RNA was extracted
from each sample, and 5--untranslated region (UTR) of BVDV
genome was amplified by using two-step reverse
transcriptase-polymerase chain reaction (RT-PCR). The PCR products
were subsequently sequenced to study the genetic variations of BVDV
in these areas. Among the 274 samples, 33 samples were found
virus-positive. According to sequence analysis of the PCR products,
the 33 samples could be arranged into 16 groups. All the sequences,
however, were highly conserved with BVDV Osloss strains. The virus
possessed theses sequences belonged to BVDV-1b subtype by
phylogenetic analysis. Based on these data, we established a typing
tree for BVDV in these areas. Our results suggested that BVDV-1b
was a predominant subgenotype in northwestern China and no
correlation between the genetic and geographical distances could be
observed above the farm level.