Abstract: Plant preparations from thyme and garlic have been shown to be effective acaricides against the poultry red mite, Dermanyssus gallinae. In a layer house with a history of D. gallinae problem, mites were detected in the monitoring traps for the first time and number of them was counted. Then, some rows of layer house was sprayed twice using a concentration of 0.21 mg/cm2 thyme essential oil and 0.07 mg/cm2 garlic juice and a similar row was used as an untreated control group. Red mite traps made of cardboard were used to assess the mite density during days 1 and 7 after treatment and always removed after 24 h. the collected mites were counted and the efficacy against all mite stages (larvae, nymphs and adults) was calculated. Results showed that on day 1 and 7 after the administration of garlic extract efficacy rate was 92.05% and 74.62%, respectively. Moreover, efficacy rate on day 1 and 7 was 89.4% and 95.37% when treatment was done with thyme essential oil. It is concluded that using garlic juice to control of D. gallinae is more effective on short time. But thyme essential oil has a long time effect in compare to garlic preparation.
Abstract: Muscid flies are known to be vectors of disease agents and species that annoy humans and domesticated animals. An example of these flies is Musca domestica (house fly) whose adult and immature stages occur in a variety of filthy organic substances including household garbage and animal manures. They contribute to microbial contamination of foods. It is therefore imperative to control these flies as a result of their role in Public health. The second and third instars of Musca domestica (Linn) were infected with varying cell loads of Bacillus subtilis in vitro for a period of 48 hours to evaluate its larvicidal activities. Mortality of the larvae increased with incubation period after treatment with the varying cell loads. Investigation revealed that the second instars larvae were more susceptible to treatment than the third instars treatments. Values obtained from the third instar group were significantly different (P
Abstract: The corn earworm, Helicoverpa zea Boddie, is a serious pest of corn. Larval feeding in ear tips destroys kernels and allows growth of fungi and production of mycotoxins. Infested sweet corn is not marketable. Development of improved transgenic hybrids expressing insecticidal toxins from Bacillus thuringiensis (Bt) may limit or prevent crop losses. The effectiveness of Attribute® II Bt resistance and applications of Voliam Xpress insecticide were evaluated for effectiveness in controlling corn earworm in plots near Urbana, IL, USA, in 2013. Where no insecticides were applied, ear infestations and kernel damage in Attribute® II ‘Protector’ plots were consistently lower (near zero) than in plots of the non-Bt isoline ‘Garrison.’ Multiple applications of Voliam Xpress significantly reduced the number of corn earworm larvae and kernel damage in the Garrison plots, but infestations and damage in these plots were greater than in Protectorplots that did not receive insecticide applications. Our results indicate that Attribute® II Bt resistance is more effective than multiple applications of an insecticide for preventing losses caused by corn earworm in sweet corn.
Abstract: The cuticular hydrocarbons of Pamphagus elephas
(Orthoptera: Pamphagidae) has been analysed by gas
chromatography and by combined gas chromatograph-mass
spectrometry. The following hydrocarbon classes have been
identified in insect cuticular hydrocarbons are: n-alkanes and
methylalkanes comprising Monomethyl-, dimethyl-and
trimethylalkanes. Sexual dimorphism is observed in long chain
alkanes (C24-C36) present on male and female. The cuticulars
hydrocarbons of P.elephas ranged from 24 to 36 carbons and
incluted n-alkanes, Dimethylalkanes and Trimethylalkanes. nalkanes
represented by (C24-C36,72,7% on male and 79,2% on
female), internally branched Monomethylalkanes identified were
(C25, C30-C32,C35-C37;11% on male and 9,4% on female),
Dimethylalkanes detected are (C31-C32, C36; 2,2% on male and
2,06% on female) and Trimethylalkanes detected are (C32, C36;
3,1% on male and 4, 97 on female). Larvae male and female (stage
7) showed the same quality of n-alkanes observed in adults.
However a difference quantity is noted.
Abstract: This study was conducted to examine the effects of ketum extract on development of Chrysomya rufifacies and to analyze the presence of mitragynine in the larvae samples. 110 newly emerged first instar larvae of C. rufifacies were introduced on ketum extract-mixed cow liver at doses of 0, 20, 40 and 60g. Blowfly development rate was determined with 12 hour intervals and
mitragynine in larvae was extracted and quantitated. C. rufifacies in control group took about 192 hours to complete their development from first instar larvae to adult blowfly; meanwhile blowfly form from the highest dose of ketum was 264 hours. Mitragynine was detected in all groups of treatment, except for control. In conclusion, the presence of mitragynine in C. rufifacies is affected in delaying development rates of the blowfly for up to 62 hours or 3 days. Chemical analysis of mitragynine from larvae samples showed that this alkaloid present in all specimens analyzed.
Abstract: The objective of this work is to explicit knowledge on the interactions between the chlorophyll-a and nine meroplankton larvae of epibenthonic fauna. The studied case is the Arraial do Cabo upwelling system, Southeastern of Brazil, which provides different environmental conditions. To assess this information a network approach based in probability estimative was used. Comparisons among the generated graphs are made in the light of different water masses, application of Shannon biodiversity index, and the closeness and betweenness centralities measurements. Our results show the main pattern among different water masses and how the core organisms belonging to the network skeleton are correlated to the main environmental variable. We conclude that the approach of complex networks is a promising tool for environmental diagnostic.
Abstract: Scolothrips longicornis Priesner is one of the
important predators of tetranychid mites with a wide distribution
throughout Iran. Life table and population growth parameters of S.
longicornis feeding on two-spotted spider mite, Tetranychus
turkestani Ugarov & Nikolski were investigated under laboratory
condition (26±1ºC, 65±5% R.H. and 16L: 8D). To carry of these
experiments, S. longicornis collections reared on cowpea infested
with T. turkestani were prepared. The eggs with less than 24 hours
old were selected and reared. The emerged larvae feeding directly on
cowpea leaf discs which were infested with T. turkestani. Thirty
females of S. longicornis with 24 hours age were selected and
released on infested leaf discs. They replaced daily to a new leaf disc
and the laying eggs have counted. The experiment continued till the
last thrips had died. The result showed that the mean age mortality of
the adult female thrips were between 21-25 days which is nearly
equal life expectancy (ex) at the time of adult eclosion. Parameters
related to reproductive table including gross reproductive rate, net
reproductive rate, intrinsic rate of natural increase and finite rate of
increase were 48.91, 37.63, 0.26 and 2.3, respectively. Mean age per
female/day, mean fertile egg per female/day, gross hatch rate, mean
net age fertility, mean net age fecundity, net fertility rate and net
fecundity rate were 2.23, 1.76, 0.87, 13.87, 14.26, 69.1 and 78.5,
respectively. Sex ratio of offspring also recorded daily. The highest
sex ratio for females was 0.88 in first day of oviposition. The sex
ratio decreased gradually and reached under 0.46 after the day 26 and
the oviposition rate declined. Then it seems that maintenance of
rearing culture of predatory thrips for mass rearing later than 26 days
after egg-laying commence is not profitable.
Abstract: The Egyptian Bacillus thuringiensis isolate (M5) produce crystal proteins that is toxic against insects was irradiated with UV light to induce mutants. Upon testing 10 of the resulting mutants for their toxicity against cotton leafworm larvae, the three mutants 62, 64 and 85 proved to be the most toxic ones. Upon testing these mutants along with their parental isolate by SDS-PAGE analysis of spores-crystals proteins as well as vegetative cells proteins, new induced bands appeared in the three mutants by UV radiation and also they showed disappearance of some other bands as compared with the wild type isolate. Multiplex PCR technique, with five sets of specific primers, was used to detect the three types of cryI genes cryIAa, cryIAb and cryIAc. Results showed that these three genes exist, as distinctive bands, in the wild type isolate (M5) as well as in mutants 62 and 85, while the mutant 64 had two distinctive bands of cryIAb and cryIAc genes, and a faint band of cryI Aa gene. Finally, these results revealed that mutant 62 is considered as the promising mutant since it is UV resistant, highly toxic against Spodoptera littoralis and active against a wide range of Lepidopteran insects.
Abstract: Experiments were carried out on the survival and growth of Rasbora daniconius, Puntius ticto and Puntius conchonius. The motivation of the study was to obtain information for growing the fish on a commercial scale for their use as biological control agents against mosquito larvae. The effects of temperature, total hardness, DO, pH and feed on the growth of fish were also investigated. Excessive value of total hardness was found because very rich calcium ion is present in Chitrakoot area. There was significant increases in growth rates of fish as temperature was increased from 280C to 300C. Further increases in temperature up to 320C, did not further affect growth. The positive and highly significant correlations 0.991488, 0.9581 and 0.9935 were found between length and weight of P. ticto, P. conchonius and R. daniconius respectively. The regression was significant at 5% level of probability.
Abstract: The toxicity of aqueous extracts of two plants,
Nicotiana tobacum and Eucalyptus globulus were investigated against second instar larvae of Lycoriella auripila, one of the most
important pests of button mushroom, using agar dilution technique.
Seven concentrations of aqueous extracts of both plants were applied
on second instar larvae and their mortality were evaluated after 24, 48 and 72 h. The obtained results revealed that aqueous extracts of N.
tabacum and E. globulus caused 77.55 and 72.5% mortality of larvae
of L. auripila at concentration of 4000 ppm after 72h, respectively. Toxicities of tobacco extract after 24, 48 and 72 h were 1.52, 1.85
and 1.70 times greather than eucalyptus, respectively. The estimated LC50 after 24, 48 and 72 h were 7316.5, 2468.5 and 2013.1 ppm for
tobacco and 64870.0, 6839.5 and 3326.4 ppm for eucalyptus, respectively. These plants merit further study as potential insecticides
for the control of L. auripila.
Abstract: In the study of chironomids in Armenia several
species of Orthocladiinae subfamily of Cricotopus genus,
Diamesinae subfamily of Diamesa genus, and Chironominae
subfamily of Chironomus genus, have been identified. In the
Cricotopus genus two sibling species were found, not distinguishable
by larval morphological features, but clearly distinct cytogenetically.
Abstract: Five original strains of entomopathogenic bacteria
with insecticidal activity against mosquito larvae of the genera Aedes,
Culex and Anopheles have been isolated from natural conditions in
Armenia and characterized. According to morphological,
physiological and biochemical parameters, all isolates were identified
as Bacillus thuringiensis spp. israelensis (Bti). High larvicidal
activity has been showed by three strains Bti. These strains can be
recommended for industrial production of bacterial preparations.
Abstract: The efficacy of the separate mixing of four tropical spicy and medicinal plant products: Dennettia tripetala Baker (pepper fruit), Eugenia aromatica Hook (clove), Piper guineense (Schum and Thonn) (black pepper) and Monodora myristica (Dunal) (African nut-meg) with a household vegetable oil was evaluated under tropical storage conditions for the control and reproductive performance of Dermestes maculatus (De Geer) (hide beetle) and Necroba rufipes (De Geer) (copra beetle) on African catfish, Clarias gariepinus (Burchell). Each of the plant materials was pulverized into powder and applied as a mix of 1ml of oil and plant powder at 2.5, 5.0, 7.5 and 10.0g per 100g of dried fish, and allowed to dry for 6h. Each of the four oil-mixed powder treatments evoked significant (P < 05) mortalities of the two insects compared with the control (oil only) at 1, 3 and 7 days post treatment. The oil-powder mixture dosages did not prevent insect egg hatchability but while the emergent larvae on the treated samples died, the emergent larvae in the control survived into adults. The application of oil-mixed powders effectively suppressed the emergence of the larvae of the beetles. Similarly, each of the oil-powder mixtures significantly reduced weight loss in smoked fish that were exposed to D. maculatus and N. rufipes when compared to the control (P < 05). The results of this study suggest that the plant powders rather than the domestic oil demonstrated protective ability against the fish beetles and confirm the efficacy of the plant products as pest control agents.
Abstract: A climate dependent model is proposed to simulate
the population of Aedes aegypti mosquito. In developing the model,
average temperature of Shah Alam, Malaysia was used to determine
the development rate of each stage of the life cycle of mosquito.
Rainfall dependent function was proposed to simulate the hatching
rate of the eggs under several assumptions. The proposed transition
matrix was obtained and used to simulate the population of eggs,
larvae, pupae and adults mosquito. It was found that the peak of
mosquito abundance comes during a relatively dry period following a
heavy rainfall. In addition, lag time between the peaks of mosquito
abundance and dengue fever cases in Shah Alam was estimated.
Abstract: Tofurther advance research on immune-related genes
from T. molitor, we constructed acDNA library and analyzed
expressed sequence taq (EST) sequences from 1,056 clones. After
removing vector sequence and quality checkingthrough thePhred
program (trim_alt 0.05 (P-score>20), 1039 sequences were generated.
The average length of insert was 792 bp. In addition, we identified 162
clusters, 167 contigs and 391 contigs after clustering and assembling
process using a TGICL package. EST sequences were searchedagainst
NCBI nr database by local BLAST (blastx, E
Abstract: Highly pathogenic avian influenza (HPAI) H5N1 viruses have created demand for a cost-effective vaccine to prevent a pandemic of the disease. Here, we report that Trichoplusia ni (T. ni) larvae can act as a cost-effective bioreactor to produce recombinant HA5 (rH5HA) proteins as an potential effective vaccine for chickens. To facilitate the recombinant virus identification, virus titer determination and access the infected larvae, we employed the internal ribosome entry site (IRES) derived from Perina nuda virus (PnV, belongs to insect picorna like Iflavirus genus) to construct a bi-cistronic baculovirus expression vector that can express the rH5HA protein and enhanced green fluorescent protein (EGFP) simultaneously. Western blot analysis revealed that the 70 kDa rH5HA protein and partially cleaved products (40 kDa H5HA1) were generated in T. ni larvae infected with recombinant baculovirus carrying the H5HA gene. These data suggest that the baculovirus-larvae recombinant protein expression system could be a cost-effective platform for H5N1 vaccine production.
Abstract: Raw wood vinegar was purified by both standing and
filtering methods. Toxicity tests were conducted under laboratory
conditions by the topical application method (contact poison) and
feeding method (stomach poison). Larvicidal activities of wood
vinegar at four different concentrations (10, 15, 20, 25 and 30 %)
were studied against second instar larvae of housefly (Musca
domestica L.). Four replicates were maintained for all treatments and
controls. Larval mortality was recorded up to 96 hours and compared
with the larval survivability by two methods of larvicidal bioassay.
Percent pupation and percent adult emergence were observed in
treated M. domestica. The study revealed that the feeding method
gave higher efficiency compared with the topical application method.
Larval mortality increased with increasing concentration of wood
vinegar and the duration of exposure. No mortality was found in
treated M. domestica larvae at minimum 10% concentration of wood
vinegar through the experiments. The treated larvae were maintained
up to pupa and adult emergence. At 30% maximum concentration
larval duration was extended to 11 days in M. domestica for topical
application method and 9 days for feeding method. Similarly the
pupal durations were also increased with increased concentrations
(16 and 24 days for topical application method and feeding method
respectively at 30% concentration) of the treatments.
Abstract: Larval rearing and seed production of most of tetra fishes (Family: Characidae) is critical due to their small size larvae and limited numbers of spawning attempts. During the present study the effect of different live foods on growth and survival of neon tetra, Paracheirodon innesi larvae (length 3.1 ± 0.012mm, weight 0.048 ± 0.00015mg) and early fry (length = 6.44 ± 0.025mm, weight = 0.64 ± 0.003mg and 13 days old) was determined in two experiments. Experiment I was conducted for rearing the larvae by using mixed green water and Infusoria whereas, in Experiment II, early fry were fed with mixed zooplankton, decapsulated Artemia cyst and Artemia nauplii. The larvae fed on mixed green water showed significant (p
Abstract: The effect of varying holding temperature on hatching success, occurrence of deformities and mortality rates were investigated for goldlined seabream eggs. Wild broodstock (600 g) were stocked at a 2:1 male-female ratio in a 2 m3 fiberglass tank supplied with filtered seawater (37 g L-1 salinity, temp. range 24±0.5 oC [day] and 22±1 oC [night], DO2 in excess of 5.0mg L-1). Females were injected with 200 IU kg-1 HCG between 08.00 and 10.00 h and returned to tanks to spawn following which eggs were collected by hand using a 100μm net. Fertilized eggs at the gastrulation stage (120 L-1) were randomly placed into one of 12 experimental 6 L aerated (DO2 5 mg L-1) plastic containers with water temperatures maintained at 24±0.5 oC (ambient), 26±0.5 oC, 28± 0.5 oC and 30±0.5 oC using thermostats. Each treatment was undertaken in triplicate using a 12:12 photophase:scotophase photoperiod. No differences were recorded between eggs reared at 24 and 26 oC with respect to viability, deformity, mortality or unhatched egg rates. Increasing temperature reduced the number of viable eggs with those at 30 oC returning poorest performance (P < 0.05). Mortality levels were lowest for eggs incubated at 24 and 26 oC. The greatest level of deformities recorded was that for eggs reared at 28 oC.
Abstract: Larval survey was carried out in 6 localities in the
urban areas (Putrajaya) and suburban areas (Kuala Selangor) from
January until December 2010. A total of 520 representative
households in 6 localities were selected. Breeding habitats were
sampled outdoors in the surroundings of housing areas. The study
indicated that the most predominant species found in both areas was
Aedes albopictus with the gardening utensil as a preferred breeding
microhabitat for Putrajaya, in contrast to the artificial containers for
Kuala Selangor. From a total of 1083 mosquito larvae species, 984
were Aedes albopictus larvae, 67 positive larvae of Aedes aegypti
and 32 of Culex larvae. Aedes Index and Container Index were
elevated in Putrajaya with 13% and 11% respectively which is higher
than the standard given by the Ministry of Health, Malaysia. This
results implicating dengue-sensitive skewed to the urban areas.
Breteau Index result also above the standard in both study locations.