Abstract: In the present study, we aimed to design the
intrauterine and extrauterine exposure to 1800 MHz GSM-like RF
radiation and investigate its possible bio-effects on infant female
rabbits. Totally thirty-six New Zealand White female rabbits, onemonth
old, were randomly divided into four groups which are
composed of 9 rabbits; i. Group I [Intrauterine (IU) exposure(-);
Extrauterine (EU) exposure (-)], Group II [IU exposure (-); EU
exposure (+)], Group III [IU exposure(+);EU exposure(-)], Group IV
[IU exposure (+);EU exposure(+)]. The master regulatory enzymes
activities of pentose phosphate pathway (glucose-6-phosphate
dehydrogenase, G-6PD; 6-phosphogluconate dehydrogenase, 6-
PGDH) and glutathione-dependent metabolism (glutathione
peroxidase, GSH-Px; glutathione reductase, GR; glutathione Stransferase,
GST, thioredoxin reductase, TRx) were analyzed in liver
tissues of young female rabbits. Decreased G-6PD, 6-PGD, GSH-Px,
GR activities were found in Group III compared to Group I (p
Abstract: Biochemical and molecular analysis of some
antioxidant enzyme genes revealed different level of gene expression
on oilseed (Brassica napus). For molecular and biochemical
analysis, leaf tissues were harvested from plants at eight different
developmental stages, from young to senescence. The levels of total
protein and chlorophyll were increased during maturity stages of
plant, while these were decreased during the last stages of plant
growth. Structural analysis (nucleotide and deduced amino acid
sequence, and phylogenic tree) of a complementary DNA revealed a
high level of similarity for a family of Catalase genes. The
expression of the gene encoded by different Catalase isoforms was
assessed during different plant growth phase. No significant
difference between samples was observed, when Catalase activity
was statistically analyzed at different developmental stages. EST
analysis exhibited different transcripts levels for a number of other
relevant antioxidant genes (different isoforms of SOD and
glutathione). The high level of transcription of these genes at
senescence stages was indicated that these genes are senescenceinduced
genes.
Abstract: Glutathione S-transferase was purified from human
erythrocytes and effects of some polyphenols were investigated on
the enzyme activity. The purification procedure was performed on
Glutathione-Agarose affinity chromatography after preparation of
erythrocytes hemolysate with a yield of 81%. The purified enzyme
showed a single band on the SDS-PAGE. The effects of some
poliphenolic compounds such as catechin, dopa, dopamine, progallol
and catechol were examined on the in vitro GST activity. Catechin
was determined to be inhibitor for the enzyme, but others were not
effective on the enzyme as inhibitors or activators. IC50 value -the
concentration of inhibitor which reduces enzyme activity by 50%-
was estimated to be 10 mM. Ki constants were also calculated as 6.38
± 0,70 mM with GSH substrate, and 3.86 ± 0,78 mM with CDNB
substrate using the equations of graphs for the inhibitor, and its
inhibition type was determined as non-competitive.
Abstract: The possible therapeutic effect of cannabidiol, the major non-psychotropic Cannabis constituent, was investigated against acute hepatotoxicity induced by a single oral dose of acetaminophen (500mg/kg) in mice. Cannabidiol (two intraperitoneal injections, 5mg/kg, each) was given 1 hour and 12 hours following acetaminophen administration. Acetaminophen administration caused significant elevations of serum alanine aminotransferase, and hepatic malondialdehyde, and nitric oxide levels, and a significant decrease in hepatic reduced glutathione. Cannabidiol significantly attenuated the deterioration in the measured biochemical parameters resulted from acetaminophen administration. Also, histopathological examination showed that cannabidiol markedly attenuated ameliorated acetaminophen-induced liver tissue damage. These results emphasize that cannabidiol represents a potential therapeutic option to protect against acetaminophen hepartotoxicity which is a common clinical problem.
Abstract: 16-Mercaptohexadecanoic acid (MHDA) and tripeptide glutathione conjugated with gold nanoparticles (Au-NPs) are characterized by Fourier Transform InfaRared (FTIR) spectroscopy combined with Surface-enhanced Raman scattering (SERS) spectroscopy. Surface Plasmon Resonance (SPR) technique based on FTIR spectroscopy has become an important tool in biophysics, which is perspective for the study of organic compounds. FTIR-spectra of MHDA shows the line at 2500 cm-1 attributed to thiol group which is modified by presence of Au-NPs, suggesting the formation of bond between thiol group and gold. We also can observe the peaks originate from characteristic chemical group. A Raman spectrum of the same sample is also promising. Our preliminary experiments confirm that SERS-effect takes place for MHDA connected with Au-NPs and enable us to detected small number (less than 106 cm-2) of MHDA molecules. Combination of spectroscopy methods: FTIR and SERS – enable to study optical properties of Au- NPs and immobilized bio-molecules in context of a bio-nano-sensors.
Abstract: This study has investigated the antidiabetic and
antioxidant potential of Pseudovaria macrophylla bark extract on
streptozotocin–nicotinamide induced type 2 diabetic rats. LCMSQTOF
and NMR experiments were done to determine the chemical
composition in the methanolic bark extract. For in vivo experiments,
the STZ (60 mg/kg/b.w, 15 min after 120 mg/kg/1 nicotinamide, i.p.)
induced diabetic rats were treated with methanolic extract of
Pseuduvaria macrophylla (200 and 400 mg/kg·bw) and
glibenclamide (2.5 mg/kg) as positive control respectively.
Biochemical parameters were assayed in the blood samples of all
groups of rats. The pro-inflammatory cytokines, antioxidant status
and plasma transforming growth factor βeta-1 (TGF-β1) were
evaluated. The histological study of the pancreas was examined and
its expression level of insulin was observed by
immunohistochemistry. In addition, the expression of glucose
transporters (GLUT 1, 2 and 4) were assessed in pancreas tissue by
western blot analysis. The outcomes of the study displayed that the
bark methanol extract of Pseuduvaria macrophylla has potentially
normalized the elevated blood glucose levels and improved serum
insulin and C-peptide levels with significant increase in the
antioxidant enzyme, reduced glutathione (GSH) and decrease in the
level of lipid peroxidation (LPO). Additionally, the extract has
markedly decreased the levels of serum pro-inflammatory cytokines
and transforming growth factor beta-1 (TGF-β1). Histopathology
analysis demonstrated that Pseuduvaria macrophylla has the
potential to protect the pancreas of diabetic rats against peroxidation
damage by downregulating oxidative stress and elevated
hyperglycaemia. Furthermore, the expression of insulin protein,
GLUT-1, GLUT-2 and GLUT-4 in pancreatic cells was enhanced.
The findings of this study support the anti-diabetic claims of
Pseudovaria macrophylla bark.
Abstract: The objective of the present study was to examine the
dose-response relationships between antioxidant parameters and liver
contaminant levels of Kazakhstan light crude oil (KLCO) in albino
rats. The animals were repeatedly exposed, by intraperitoneal
injection, to low dosages (0.5–1.5 ml/kg) of KLCO. Rats exposed to
these doses levels did not show any apparent symptoms of
intoxication. Serum aminotransferases increased significantly
(p
Abstract: Biochemical investigations were carried out to assess
the effect of different exposure regimes of Kazakhstan crude oil
(KCO) on hepatic antioxidant defense system in albino rats.
Contaminants were delivered under two different dosing regimes,
with all treatments receiving the same total contaminant load by the
end of the exposure period. Rats in regime A injected with KCO
once at a dose of 6 ml/kg bw while in regime B injected multiply at a
dose of 1.5 ml/kg bw on day 1, 3, 5 and 8. Antioxidant biomarkers
were measured in hepatic tissue after 1, 3, 5 and 8 days. Significant
induction was observed in serum aminotransferases (ALT, AST)
(p
Abstract: Delayed wound healing in diabetes is primarily
associated with hyperglycemia, over-expression of inflammatory
marker, oxidative stress and delayed collagen synthesis. This
unmanaged wound is producing high economic burden on the
society. Thus research is required to develop new and effective
treatment strategies to deal with this emerging issue. Our present
study incorporates the evaluation of wound healing effects of 50%
ethanol extract of Ocimum sanctum (OSE) in streptozotocin
(45mg/kg)-induced diabetic rats with concurrent wound ulcer. The
animals showing diabetes (Blood glucose level >140 and
Abstract: In this study, we investigated the effects of ginger and
L-carnitine on the reproductive performance of male rats with respect
to semen parameters, male sex hormones and the testicular
antioxidant system. A total of sixty mature male albino rats were
divided into four groups of fifteen rats. The control group received
saline, whereas the other three groups received ginger (100 mg kg-1 d-
1.), L-carnitine (150 mg kg-1 d-1.) or a combination of both ginger
(100 mg kg-1 d-1.) and L-carnitine (150 mg kg-1 d-1.) via a stomach
tube daily for one month. At the end of the treatment period, the rats
were sacrificed, and their sperm characteristics (count, motility and
viability), antioxidant enzyme factors levels (reduced glutathione,
catalase, superoxide dismutase and total antioxidant capacity) and sex
hormone levels (testosterone, Follicle stimulating hormone(FSH) and
luteinizing hormone (LH) were analysed. Our results showed that the
three experimental treatments improved sperm parameters,
antioxidant enzyme activity and testosterone hormone levels; the
most pronounced positive effects were observed in the group that
received a combination of both ginger and L-carnitine. Therefore, the
administration of a combination of ginger and L-carnitine may be
beneficial for improving male sexual performance.
Abstract: Kombucha Tea Ferment (KT), was given to male
albino rats, (1ml/Kg of body weight), via gavages, during 2 weeks
before intraperitoneal administration of 3.5 mg/Kg body weight
CdCl2 and/or whole body γ-irradiation with 4Gy, and during 4 weeks
after each treatment. Hepatic and nephritic pathological changes
included significant increases of serum alanine transaminase (ALT),
aspartate transaminase (AST), and alkaline phosphatase (ALP)
activities, and creatinine and urea contents with significant decrease
in serum total antioxidant capacity (TAC). Increase in oxidative
stress markers in liver and kidney tissues expressed by significant
increase in malondialdehyde (MDA) and nitric oxide (NO) contents
associated to significant depletion in superoxide dismutase (SOD)
and catalase (CAT) activities, and reduced glutathione (GSH) content
were recorded. KT administration results in recovery of all the
pathological changes. It could be concluded that KT might protect
liver and kidney from oxidative damage induced by exposure to
cadmium and/ or γ-irradiation.
Abstract: The use of bioindicators plants (lichens, bryophytes
and Sphagnum....) in monitoring pollution by heavy metals has been
the subject of several works. However, few studies have addressed
the impact of specific type-s pollutants (fertilizers, pesticides.) on
these organisms.
We propose in this work to make the highlighting effect of NPKs
(NPK: nitrogen-phosphate-potassium-sulfate (NP2O5K2O) (15,15,15),
at concentrations of 10, 20, 30 , 40 and 50mM/L) on the activity of
detoxification enzymes (GSH/GST, CAT, APX and MDA) of plant
bioindicators (mosses and lichens) after treatment for 3 and 7 days.
This study shows the important role of the defense system in the
accumulation and tolerance to chemical pollutants through the
activation of enzymatic (GST (glutathione-S-transferase, APX
(ascorbat peroxidase), CAT (catalase)) and nonenzymatic biomarkers
(GSH (glutathione), MDA (malondialdehyde)) against oxidative
stress generated by the NPKs.