Abstract: Calcium is an essential element for good growth and development of the organism, and its requirement is increased at school age. Low socio-economic populations of developing countries such as Colombia may have food deficiency of this mineral in schoolchildren that could be reflected in calcium biochemical indicators, bone alterations and anthropometric indicators. The objective of this investigation was to evaluate some calcium biochemical indicators in a group of schoolchildren of low socioeconomic level from Barranquilla city and to correlate with body mass index. 60 schoolchildren aged 7 to 15 years were selected from Jesus’s Heart Educational Institution in Barranquilla-Atlántico, apparently healthy, without suffering from infectious or gastrointestinal diseases, without habits of drinking alcohol or smoking another hallucinogenic substance and without taking supplementation with calcium in the last six months or another substance that compromises bone metabolism. The research was approved by the ethics committee at Universidad del Atlántico. The selected children were invited to donate a blood and urine sample in a fasting time of 12 hours, the serum was separated by centrifugation and frozen at ˗20 ℃ until analyzed and the same was done with the urine sample. On the day of the biological collections, the weight and height of the students were measured to determine the nutritional status by BMI using the WHO tables. Calcium concentrations in serum and urine (SCa, UCa), alkaline phosphatase activity total and of bone origin (SAPT, SBAP) and urinary creatinine (UCr) were determined by spectrophotometric methods using commercial kits. Osteocalcin and Cross-linked N-telopeptides of type I collagen (NTx-1) in serum were measured with an enzyme-linked inmunosorbent assay. For statistical analysis the Statgraphics software Centurium XVII was used. 63% (n = 38) and 37% (n = 22) of the participants were male and female, respectively. 78% (n = 47), 5% (n = 3) and 17% (n = 10) had a normal, malnutrition and high nutritional status, respectively. The averages of evaluated indicators levels were (mean ± SD): 9.50 ± 1.06 mg/dL for SCa; 181.3 ± 64.3 U/L for SAPT, 143.8 ± 73.9 U/L for SBAP; 9.0 ± 3.48 ng/mL for osteocalcin and 101.3 ± 12.8 ng/mL for NTx-1. UCa level was 12.8 ± 7.7 mg/dL that adjusted with creatinine ranged from 0.005 to 0.395 mg/mg. Considering serum calcium values, approximately 7% of school children were hypocalcemic, 16% hypercalcemic and 77% normocalcemic. The indicators evaluated did not correlate with the BMI. Low values were observed in calcium urinary excretion and high in NTx-1, suggesting that mechanisms such as increase in renal retention of calcium and in bone remodeling may be contributing to calcium homeostasis.
Abstract: The objective of the present study was to investigate the effect of green tea extract on serum oxidant and antioxidant profile, liver and kidney function. 40 Friesian calves are included in this study and allocated into two groups: Group I (n=20) clinically healthy calves showing no clinical abnormalities, not receiving any treatment and served as control; group II (n=20) received green tea extract (GTE) for 30 days. Non-significant changes in blood urea nitrogen (BUN) were detected between groups, on contrary, serum creatinine and activities of liver enzymes aspartate transaminase (AST) and alanine transaminase (ALT) were significantly different between two groups. There were significant increases in the mean values of serum antioxidative parameters (total antioxidant capacity, catalase, superoxide dismutase, reduced glutathione and glutathione peroxidase) in group II. Whereas, the activity of lipid peroxidase significantly decreased in GTE treated calves when compared to control.
Abstract: This work aimed to examine Oreochromis niloticus fish from Lake Manzala in Port Said, Dakahlya and Damietta governorates, Egypt, as a bio-indicator for the lake water pollution through recording alterations in their hematological, physiological, and histopathological parameters. All fish samples showed a significant increase in levels of alkaline phosphatase (ALP), creatinine and glutathione-S-transferase (GST); only Dakahlya samples showed a significant increase (p
Abstract: Intense oxidative stress, increased glycated hemoglobin and mineral imbalance represent risk factors for complications in diabetic patients. Cardiovascular complications are most common in these patients, including nephropathy. This study was conducted in 2015 at the Procardia Laboratory in Tîrgu Mureș, Romania on 40 type 2 diabetic adults. Routine biochemical tests were performed on the Konleab 20XTi analyzer (serum glucose, total cholesterol, LDL and HDL cholesterol, triglyceride, creatinine, urea). We also measured serum uric acid, magnesium and calcium concentration by photometric procedures, potassium, sodium and chloride by ion selective electrode, and chromium by atomic absorption spectrometry in a group of patients. Glycated hemoglobin (HbA1c) dosage was made by reflectometry. Urine analysis was performed using the HandUReader equipment. The level of oxidative stress was measured by serum malondialdehyde dosage using the thiobarbituric acid reactive substances method. MDRD (Modification of Diet in Renal Disease) formula was applied for calculation of creatinine-derived glomerular filtration rate. GraphPad InStat software was used for statistical analysis of the data. The diabetic subject included in the study presented high MDA concentrations, showing intense oxidative stress. Calcium was deficient in 5% of the patients, chromium deficiency was present in 28%. The atherogenic cholesterol fraction was elevated in 13% of the patients. Positive correlation was found between creatinine and MDRD-creatinine values (p
Abstract: The present study investigated the protective effect of
thymoquinone (TQ), against cadmium-induced kidney injury in rats.
Cadmium chloride (1.2 mg Cd/kg/day, s.c.), was given for nine
weeks. TQ treatment (40 mg/kg/day, p.o.) started on the same day of
cadmium administration and continued for nine weeks. TQ
significantly decreased serum creatinine, renal malondialdehyde and
nitric oxide, and significantly increased renal reduced glutathione in
rats received cadmium. Histopathological examination showed that
TQ markedly minimized renal tissue damage induced by cadmium.
Immunohistochemical analysis revealed that TQ markedly decreased
the cadmium-induced expression of inducible nitric oxide synthase,
tumor necrosis factor-α, cyclooxygenase-2, and caspase-3 in renal
tissue. It was concluded that TQ significantly protected against
cadmium nephrotoxicity in rats, through its antioxidant, antiinflammatory,
and antiapoptotic actions.
Abstract: The aim of the study was to investigate the effect of
Saccharomyces cerevisiae (SC) live yeast culture on microbial
protein supply to small intestine in Kivircik male yearlings when fed
with different ratio of forage and concentrate diets. Four Kivircik
male yearlings with permanent rumen canula were used in the
experiment. The treatments were allocated to a 4x4 Latin square
design. Diet I consisted of 70% alfalfa hay and 30% concentrate, Diet
II consisted of 30% alfalfa hay and 70% concentrate, Diet I and II
were supplemented with a SC. Daily urine was collected and stored at
-20°C until analysis. Calorimetric methods were used for the
determination of urinary allantoin and creatinine levels. The
estimated microbial N supply to small intestine for Diets I, I+SC, II
and II+SC were 2.51, 2.64, 2.95 and 3.43 g N/d respectively.
Supplementation of Diets I and II with SC significantly affected the
allantoin levels in μmol/W0.75 (p
Abstract: This study aimed to determine the possible protective effects of L‐carnitine against gentamicin‐induced nephrotoxicity. Forty male albino rats were divided into 4 groups (10 rats each); Group 1: normal control, group 2: induced nephrotoxicity (gentamicin 50 mg/kg/day S.C; 8 days), group 3: treated with L‐ carnitine (40 mg/kg/d SC for 12 days) and group 4: treated with L‐ carnitine 4 days before and for 8 days in concomitant with gentamicin. Gentamicin‐induced nephrotoxicity (group 2): caused significant increase in serum urea, creatinine, urinary N‐acetyl‐B‐D‐ glucosaminidase (NAG), gamma glutamyl transpeptidase (GGT), urinary total protein and kidney tissue malondialdehyde (MDA) with significant decrease in serum superoxide dismutase (SOD), serum catalase and creatinine clearance and marked tubular necrosis in the proximal convoluted tubules with interruption in the basement membrane around the necrotic tubule compared to the normal control group. L‐carnitine 4 days before and for 8 days in concomitant with gentamicin (group 4) offered marked decrease in serum urea, serum creatinine, urinary NAG, urinary GGT, urinary proteins and kidney tissue MDA, with marked increase in serum SOD, serum catalase and creatinine clearance with marked improvement in the tubular damage compared to gentamicin‐induced nephrotoxicity group. L‐carnitine administered for 12 days produced no change in the parameters mentioned above as compared to the normal control group. In conclusion: L‐carnitine could reduce most of the biochemical parameters and also improve the histopathological features of kidney asscociated with gentamicin induced‐nephrotoxicity.
Abstract: This study was conducted to investigate the effect of the leaf essential oil of C. sinensis harvested at 7.00a.m and 4.00p.m on some Liver and Kidney function indices of diabetic rats as well as investigate the effect of time of harvest on the observed effect. Experimental animals were divided into 4 groups (A, B, C and D). Diabetes mellitus was induced in all animals, except the normal control group (Group A), by injecting 150mg/kg body weight of alloxan monohydrate intraperitoneally. Group A received distilled water while group B (diabetic control group) was not treated. Group C and D were treated with leaf essential oil of C. sinensis harvested at 7.00 a.m and 4.00p.m respectively at a dose of 110 mg/kg body weight every other day for 15 days. Alkaline phosphatase (ALP), Alanine Transaminase (ALT) and Aspartate Transaminase (AST) activity was evaluated in the serum, Liver and Kidney of studied animals. Total and Direct Bilirubin level, Total Protein and Globulin, Creatinine and Urea level were also evaluated. Result showed that creatinine and urea, serum ALP, AST and ALT levels was significantly reduced (p < 0.05), while the levels of total Protein and Globulin increased significantly (p < 0.05) for the treated animals compared to the diabetic control group. In conclusion, the leaf essential oil of Citrus sinensis ameliorated the impaired renal and liver function; however, the time of harvest of the leaf does not significantly affect its ameliorative effect.
Abstract: The objectives of the present study were to estimate the microbial-N flow to the small intestine and to predict the digestible organic matter intake (DOMI) in grazing Karayaka sheep based on urinary excretion of purine derivatives (xanthine, hypoxanthine, uric acid, and allantoin) by the use of spot urine sampling under field conditions. In the trial, 10 Karayaka sheep from 2 to 3 years of age were used. The animals were grazed in a pasture for ten months and fed with concentrate and vetch plus oat hay for the other two months (January and February) indoors. Highly significant linear and cubic relationships (P
Abstract: This study was conducted to evaluate the anti-diabetic
properties of ethanolic extract of two plants commonly used in folk
medicine, Mormodica charantia (bitter melon) and Trigonella
foenum-graecum (fenugreek). The study was performed on STZinduced
diabetic rats (DM type-I). Plant extracts of these two plants
were given to STZ diabetic rats at the concentration of 500 mg/kg
body weight ,50 mg/kg body weight respectively. Cidophage®
(metformin HCl) were administered to another group to support the
results at a dose of 500 mg/kg body weight, the ethanolic extracts and
Cidophage administered orally once a day for four weeks using a
stomach tube and; serum samples were obtained for biochemical
analysis. The extracts caused significant decreases in glucose levels
compared with diabetic control rats. Insulin secretions were increased
after 4 weeks of treatment with Cidophage® compared with the
control non-diabetic rats. Levels of AST and ALT liver enzymes were
normalized by all treatments. Decreases in liver cholesterol,
triglycerides, and LDL in diabetic rats were observed with all
treatments. HDL levels were increased by the treatments in the
following order: bitter melon, Cidophage®, and fenugreek. Creatinine
levels were reduced by all treatments. Serum nitric oxide and
malonaldehyde levels were reduced by all extracts. GSH levels were
increased by all extracts. Extravasation as measured by the Evans
Blue test increased significantly in STZ-induced diabetic animals.
This effect was reversed by ethanolic extracts of bitter melon or
fenugreek.
Abstract: This study examined the toxicological effects and
safety of polypeptide k isolated from the seeds of Momordica
charantia in laboratory rats. 30 male Sprague Dawley rats (12 weeks
old, bodyweight 180-200 g) were randomly divided into 3 groups
(1000 mg/kg, 500 mg and 0 mg/kg). Rats were acclimatized to
laboratory conditions for 7 days and at day 8 rats were dosed orally
with polypeptide k (in 2% DMSO/normal saline) and the controls
received the dosed vehicle only. Rats were then observed for 72
hours before sacrificed. Rats were anaesthetized by pentobarbital
(50 mg/kg ip) and 2-3.0 mL of blood was taken by cardiac puncture
and rats were scarified by anaesthetic overdose. Immediately, organs
(heart, lungs, liver, kidneys) were weigh and taken for histology.
Organ sections were then evaluated by a histopathologist. Serum
samples were assayed for liver functions (ALT and γ-GT) and kidney
functions (BUN and creatinine). All rats showed normal behavior
after the dosing and no statistical changes were observed in all blood
parameters and organ weight. Histological examinations revealed
normal organ structures. In conclusion, dosing of rats up to 1000
mg/kg did not have any effects on the rat behavior, liver or kidney
functions nor histology of the selected organs.
Abstract: This paper presents the prediction of kidney
dysfunction using different neural network (NN) approaches. Self
organization Maps (SOM), Probabilistic Neural Network (PNN) and
Multi Layer Perceptron Neural Network (MLPNN) trained with Back
Propagation Algorithm (BPA) are used in this study. Six hundred and
sixty three sets of analytical laboratory tests have been collected from
one of the private clinical laboratories in Baghdad. For each subject,
Serum urea and Serum creatinin levels have been analyzed and tested
by using clinical laboratory measurements. The collected urea and
cretinine levels are then used as inputs to the three NN models in
which the training process is done by different neural approaches.
SOM which is a class of unsupervised network whereas PNN and
BPNN are considered as class of supervised networks. These
networks are used as a classifier to predict whether kidney is normal
or it will have a dysfunction. The accuracy of prediction, sensitivity
and specificity were found for each type of the proposed networks
.We conclude that PNN gives faster and more accurate prediction of
kidney dysfunction and it works as promising tool for predicting of
routine kidney dysfunction from the clinical laboratory data.
Abstract: This study was designed to investigate the role of serum nitric oxide and sialic acid in the development of diabetic nephropathy as disease marker. Total 210 diabetic patients (age and sex matched) were selected followed by informed consent and divided into four groups (70 each) as I: control; II: diabetic; III: diabetic hypertensive; IV: diabetic nephropathy. The blood samples of all subjects were collected and analyzed for serum nitric oxide, sialic acid, fasting blood glucose, serum urea, creatinine, HbA1c and GFR. The BMI, systolic and diastolic blood pressures, blood glucose, HbA1c and serum sialic acid levels were high (p
Abstract: The adverse effects of Clindamycin (Clind.) /
Ibuprofen (Ibu.) combination on liver, kidney, blood elements and the
significances of antioxidants (N-acetylcysteine and Zinc) against
these effects were evaluated. The study includes: Group I; control
n=30, Group II; patients on Clind.300mg/Ibu.400mg twice daily for a
week n=30, Group III; patients on Clind.300mg/Ibu.400mg+Nacetylcysteine
200mg twice daily for a week n=15 and Group IV;
patients on Clind.300mg/Ibu.400mg+Zinc50mg twice daily for a
week n=15. Serum malondialdehyde (MDA), alanine transferase
(ALT), aspartate transferase (AST), γ glutamyl transferase (GGT),
creatinine, blood urea nitrogen (BUN) were measured. Applying one
way ANOVA followed by Tuckey Kramer post test, Group II showed
significant increase in ALT, AST, GGT, BUN and decrease in Hb,
RBCs, platelets than Group I. Group III showed significant decrease
in ALT, AST, GGT, BUN than Group II. Moreover, Group IV
showed significant decrease in ALT, AST, GGT and increase in Hb,
RBCs, and platelets than Group II. Conclusively, Adding Zinc or Nacetylcysteine
buffer the oxidative stress and improve the therapeutic
outcome of Clindamycin/Ibuprofen combination.
Abstract: In the present research, two nutraceuticals made from
red grape and walnut that showed previously to improve kidney
dysfunction were incorporated separately into functional foods' bread
made from barley and rice bran. The functional foods were evaluated
in rats in which chronic renal failure was induced through feeding
diet rich in adenine and phosphate (APD). The evaluation based on
assessing kidney function, oxidative stress, inflammatory biomarkers
and body weight gain. Results showed induction of chronic kidney
failure reflected in significant increase in plasma urea, creatinine,
malondialdehyde, tumor necrosis factor- α and low density
lipoprotein cholesterol along with significant reduction of plasma
albumin, and total antioxidant and creatinine clearance and body
weight gain on feeding APD compared to control healthy group.
Feeding the functional foods produced amelioration in the different
biochemical parameters and body weight gain indicating
improvement in kidney function.
Abstract: Acute kidney injury (AKI) is a new worldwide public
health problem. A diagnosis of this disease using creatinine is still a
problem in clinical practice. Therefore, a measurement of biomarkers
responsible for AKI has received much attention in the past couple
years. Cytokine interleukin-18 (IL-18) was reported as one of the
early biomarkers for AKI. The most commonly used method to
detect this biomarker is an immunoassay. This study used a planar
platform to perform an immunoassay using fluorescence for
detection. In this study, anti-IL-18 antibody was immobilized onto a
microscope slide using a covalent binding method. Make-up samples
were diluted at the concentration between 10 to 1000 pg/ml to create
a calibration curve. The precision of the system was determined
using a coefficient of variability (CV), which was found to be less
than 10%. The performance of this immunoassay system was
compared with the measurement from ELISA.
Abstract: Glomerular filtration rate (GFR) is a measure of
kidney function. It is usually estimated from serum concentrations of
cystatin C or creatinine although there has been considerable debate
in the literature about (i) the best equation to use and (ii) the
variability in the correlation between the concentrations of creatinine
and cystatin C. The equations for GFR can be written in a general
form and from these I calculate the error of the GFR estimates
associated with analyte measurement error. These show that the
error of the GFR estimates is such that it is not possible to distinguish
between the equations over much of the concentration range of either
analyte. The general forms of the equations are also used to derive
an expression for the concentration of cystatin C as a function of the
concentration of creatinine. This equation shows that these analyte
concentrations are not linearly related. Clinical reports of cystatin C
and creatinine concentration are consistent with the expression
derived.
Abstract: Life is beautiful. But, it is decided by genes, environment and the individual and shattered by the natural and / or the invited problems. Most of the global rural helpless masses are struggling for their survival since; they are neglected in all aspects of life including health. Amidst a countless number of miserable diseases in man, diabetes is becoming a dreaded killer and ramifying the entire globe in a jet speed. Diabetes control continues as a Herculean task to the scientific community and the modern society in the 21st century also. T2DM is not pertaining to any age and it can develop even during the childhood. This multifactorial disease abruptly changes the activities of certain vital biomarkers in the present rural T2DM cases. A remarkable variation in the levels of biomarkers like AST, ALT, GGT, ALP, LDH, HbA1C, C- peptide, fasting sugar, post-prandial sugar, sodium, potassium, BUN, creatinine and insulin show the rampant nature of T2DM in this physically active rural agrarian community.
Abstract: Kombucha Tea Ferment (KT), was given to male
albino rats, (1ml/Kg of body weight), via gavages, during 2 weeks
before intraperitoneal administration of 3.5 mg/Kg body weight
CdCl2 and/or whole body γ-irradiation with 4Gy, and during 4 weeks
after each treatment. Hepatic and nephritic pathological changes
included significant increases of serum alanine transaminase (ALT),
aspartate transaminase (AST), and alkaline phosphatase (ALP)
activities, and creatinine and urea contents with significant decrease
in serum total antioxidant capacity (TAC). Increase in oxidative
stress markers in liver and kidney tissues expressed by significant
increase in malondialdehyde (MDA) and nitric oxide (NO) contents
associated to significant depletion in superoxide dismutase (SOD)
and catalase (CAT) activities, and reduced glutathione (GSH) content
were recorded. KT administration results in recovery of all the
pathological changes. It could be concluded that KT might protect
liver and kidney from oxidative damage induced by exposure to
cadmium and/ or γ-irradiation.