Abstract: This study examined the toxicological effects and
safety of polypeptide k isolated from the seeds of Momordica
charantia in laboratory rats. 30 male Sprague Dawley rats (12 weeks
old, bodyweight 180-200 g) were randomly divided into 3 groups
(1000 mg/kg, 500 mg and 0 mg/kg). Rats were acclimatized to
laboratory conditions for 7 days and at day 8 rats were dosed orally
with polypeptide k (in 2% DMSO/normal saline) and the controls
received the dosed vehicle only. Rats were then observed for 72
hours before sacrificed. Rats were anaesthetized by pentobarbital
(50 mg/kg ip) and 2-3.0 mL of blood was taken by cardiac puncture
and rats were scarified by anaesthetic overdose. Immediately, organs
(heart, lungs, liver, kidneys) were weigh and taken for histology.
Organ sections were then evaluated by a histopathologist. Serum
samples were assayed for liver functions (ALT and γ-GT) and kidney
functions (BUN and creatinine). All rats showed normal behavior
after the dosing and no statistical changes were observed in all blood
parameters and organ weight. Histological examinations revealed
normal organ structures. In conclusion, dosing of rats up to 1000
mg/kg did not have any effects on the rat behavior, liver or kidney
functions nor histology of the selected organs.
Abstract: Currently, there is no database or local norms for the
physical performance of Malaysian rugby players. This database or
norms are vital for Malaysian-s sports development as programs can
be setup to improve the current status. This pilot study was
conducted to evaluate the status of our semi professional rugby
players. The rugby players were randomly selected from the
Malaysian National team and several clubs in the Klang valley, Kuala
Lumpur Malaysia. 54 male rugby players (Age: 24.41 ± 4.06 years)
were selected for this pilot study. Height, bodyweight, percentage
body fat and body mass index (BMI) and several other physical tests
were performed. Results from the BLEEP test revealed an average of
level 9, shuttle 2 for the players. Interestingly, forwards were taller,
heavier, and had lower maximal aerobic power than backs in the
same team. In conclusion, the physical characteristics of the rugby
players were much lower when compared to international players
from other countries. From this pilot study, the physical performance
of the Malaysian team must be improved in order to further develop
the sports.