Development of an Immunoassay Platform for Diagnosis of Acute Kidney Injury
Acute kidney injury (AKI) is a new worldwide public
health problem. A diagnosis of this disease using creatinine is still a
problem in clinical practice. Therefore, a measurement of biomarkers
responsible for AKI has received much attention in the past couple
years. Cytokine interleukin-18 (IL-18) was reported as one of the
early biomarkers for AKI. The most commonly used method to
detect this biomarker is an immunoassay. This study used a planar
platform to perform an immunoassay using fluorescence for
detection. In this study, anti-IL-18 antibody was immobilized onto a
microscope slide using a covalent binding method. Make-up samples
were diluted at the concentration between 10 to 1000 pg/ml to create
a calibration curve. The precision of the system was determined
using a coefficient of variability (CV), which was found to be less
than 10%. The performance of this immunoassay system was
compared with the measurement from ELISA.
[1] W. K. Han and J. V. Bonventre, "Biologic Markers for the Early
Detection of Acute Kidney Injury," Current Opinion in Critical Care,
Vol. 10, 2004, pp. 476-482.
[2] M.R. Lattanzio and N.P. Kopyt, "Acute Kidney Injury: New Concepts in
Definition, Diagnosis,Pathophysiology, and Treatment," Journal of the
American Osteopathic Association, Vol. 109 No.1, 2009, pp. 13-19.
[3] S.S. Soni, C. Ronco, N. Katz and D.N. Cruz, "Early Diagnosis of Acute
Kidney Injury: The Promise of Novel Biomarkers," Blood Purification,
Vol. 28, 2009, pp. 165-174.
[4] R. Bellomo, J.A. Kellum, and C. Ronco, "Defining Acute Renal Failure:
Physiological Principles," Intensive Care Medicine, Vol. 30, 2004, pp.
33-37.
[5] L.-M. Barbara, "Serum and Urinary Biomarkers of Acute Kidney
Injury," Blood Purification, Vol. 29, 2010, pp. 357-365.
[6] K.D. Liu, "Molecular mechanisms of recovery from acute renal failure,"
Crit Care Med, Vol. 31, 2003, pp.72-581.
[7] C.R. Parikh, E. Abraham, M. Ancukiewicz, and C.L. Edelstein, "Urine
Il-18 Is an Early Diagnostic Marker for Acute Kidney Injury and
Predicts Mortality in the Intensive Care Unit," Journal of the American
Society of Nephrology, Vol. 16, 2005, pp. 3046-3052.
[8] M.A. Ferguson, V.S. Vaidya and J.V. Bonventre, "Biomarkers of
Nephrotoxic Acute Kidney Injury," Toxicology, Vol. 245, 2008, pp.
182-193.
[9] C.R. Parikh, A. Janib, V.Y. Melnikov, S. Faubel and C.L. Edelstein,
"Urinary Interleukin-18 Is a Marker of Human Acute Tubular Necrosis,"
American Journal of Kidney Diseases, Vol. 43 No.3, 2004, pp. 405-414.
[10] J.F. Naud and M. Leblanc, "Biomarkers in Acute Kidney Injury,"
Biomarker Insights, Vol.3, 2008, pp.115-125.
[11] V.S. Vaidya, M.A. Ferguson, and J.V. Bonventre, J.V., "Biomarkers of
Acute Kidney Injury," Annual Review Pharmacology and Toxicology,
Vol. 48, 2008, pp. 463-493.
[12] N. Karoonuthaisiri, R. Charlermroj, U. Uawisetwathana, P. Luxananil,
K. Kirtikara and O. Gajanandana, "Development of antibody array for
simultaneous detection of foodborne pathogens," Biosensors and
Bioelectronics, Vol. 24, 2009, pp. 1641-1648.
[13] N. Bojorge Ramírez, A.M. Salgado, and B. Valdman, "The Evolution
and Developments Sensors for Health and Environmental Monitoring:
Problems and Perspectives," Brazilian Journal of Chemical
Engineering, Vol. 26 No. 2, 2008, pp. 227-249.
[14] Y. Jung, J.Y. Jeong, and B.H. Chung, "Recent Advances in
Immobilization Methods of Antibodies on Solid Supports," Analyst, Vol.
133, 2008, pp. 697-701.
[15] E.W. Olle, J. Messamore, M.P. Deogracias, S.D. McClintock, T.D.
Anderson, and K.J. Johnson, "Comparison of Antibody Array Substrates
and the Use of Glycerol to Normalize Spot Morphology," Experimental
and Molecular Pathology, Vol. 79, 2005, pp. 206 - 209.
[16] S.L. Seurynck-Servoss, C.L. Baird, K.D. Rodland, and R.C. Zangar,
"Surface Chemistries for Antibody Microarrays," Frontiers in
bioscience, Vol. 12, 2007, pp. 3956-3964.
[17] E.W. Olle, M.P. Deogracias, J.E. Messamore, S.D. McClintock, A.G.
Barron, T.D. Anderson, and K.J. Johnson, 2007, "Screening of Serum
Samples from Wegener-s Granulomatosis Patients Using Antibody
Microarrays," Proteomics clinical application, Vol. 1, pp. 1212-1220.
[18] M.G.R. Rajan, and B. Gupta, "Immobilization of Antibody Spots on
Glass Surfaces - Potential Use as Antibody Chips for Multianalyte
Assays," BARC Newsletter, Vol. 10 No.237, 2003, pp. 107-110.
[19] D. Maraldo, and R. Mutharasan, "Optimization of Antibody
Immobilization for Sensing Using Piezoelectrically Excited-Millimeter-
Sized Cantilever (Pemc) Sensors," Sensors and Actuators B, Vol. 123,
2007, pp. 474-479.
[20] G. Lonnemann, D. Novick, M. Rubinstein and C. Dinarello,
"Interleukin-18, Interleukin-18 Binding Protein and Impaired Production
of Interferon-Gamma in Chronic Renal Failure," Clinical Nephrology,
Vol. 60 No.5, 2003, pp. 327-334.
[21] C.R. Parikh, A. Janib, J. Mishra, Q. Ma, C. Kelly, J. Barasch, C.L.
Edelstein, and P. Devarajan, "Urine Ngal and Il-18 Are Predictive
Biomarkers for Delayed Graft Function Following Kidney
Transplantation," American Journal of Transplantation, Vol. 6, 2006,
pp. 1639-1645.
[22] C.R. Parikh, J. Mishra, H. Thiessen-Philbrook, B. Dursun, Q. Ma, C.
Kelly, C. Dent, P. Devarajan and C. Edelstein, "Urinary Il-18 Is an Early
Predictive Biomarker of Acute Kidney Injury after Cardiac Surgery,"
Kidney International, Vol. 70, 2006, pp. 199-203.
[23] D. Hu, X. Liu, S. Chen and C. Bao, "Expressions of Il-18 and Its
Binding Protein in Peripheral Blood Leukocytes and Kidney Tissues of
Lupus Nephritis Patients," Clinical Rheumatol, Vol. 29, 2010, pp. 717-
721.
[24] B. DeSilva, W. Smith, R. Weiner, M. Kelley, J. Smolec, B. Lee, M.
Khan, R. Tacey, H. Hill and A. Celniker, "Recommendations for the
bioanalytical method validationof ligand-binding assays to support
pharmacokinetic assessments of macromolecules," Pharmaceutical
Research, Vol. 20, 2003, pp. 1885-1900.
[25] M. Bonham and S. Miller, "Clinical Comparison of 99th Percentile and
10% Coefficient of Variation Cutoff Values for Four Commercially
Available Troponin I Assays," LABMEDICINE, vol. 4o No.8, 2009, pp.
470-473.
[1] W. K. Han and J. V. Bonventre, "Biologic Markers for the Early
Detection of Acute Kidney Injury," Current Opinion in Critical Care,
Vol. 10, 2004, pp. 476-482.
[2] M.R. Lattanzio and N.P. Kopyt, "Acute Kidney Injury: New Concepts in
Definition, Diagnosis,Pathophysiology, and Treatment," Journal of the
American Osteopathic Association, Vol. 109 No.1, 2009, pp. 13-19.
[3] S.S. Soni, C. Ronco, N. Katz and D.N. Cruz, "Early Diagnosis of Acute
Kidney Injury: The Promise of Novel Biomarkers," Blood Purification,
Vol. 28, 2009, pp. 165-174.
[4] R. Bellomo, J.A. Kellum, and C. Ronco, "Defining Acute Renal Failure:
Physiological Principles," Intensive Care Medicine, Vol. 30, 2004, pp.
33-37.
[5] L.-M. Barbara, "Serum and Urinary Biomarkers of Acute Kidney
Injury," Blood Purification, Vol. 29, 2010, pp. 357-365.
[6] K.D. Liu, "Molecular mechanisms of recovery from acute renal failure,"
Crit Care Med, Vol. 31, 2003, pp.72-581.
[7] C.R. Parikh, E. Abraham, M. Ancukiewicz, and C.L. Edelstein, "Urine
Il-18 Is an Early Diagnostic Marker for Acute Kidney Injury and
Predicts Mortality in the Intensive Care Unit," Journal of the American
Society of Nephrology, Vol. 16, 2005, pp. 3046-3052.
[8] M.A. Ferguson, V.S. Vaidya and J.V. Bonventre, "Biomarkers of
Nephrotoxic Acute Kidney Injury," Toxicology, Vol. 245, 2008, pp.
182-193.
[9] C.R. Parikh, A. Janib, V.Y. Melnikov, S. Faubel and C.L. Edelstein,
"Urinary Interleukin-18 Is a Marker of Human Acute Tubular Necrosis,"
American Journal of Kidney Diseases, Vol. 43 No.3, 2004, pp. 405-414.
[10] J.F. Naud and M. Leblanc, "Biomarkers in Acute Kidney Injury,"
Biomarker Insights, Vol.3, 2008, pp.115-125.
[11] V.S. Vaidya, M.A. Ferguson, and J.V. Bonventre, J.V., "Biomarkers of
Acute Kidney Injury," Annual Review Pharmacology and Toxicology,
Vol. 48, 2008, pp. 463-493.
[12] N. Karoonuthaisiri, R. Charlermroj, U. Uawisetwathana, P. Luxananil,
K. Kirtikara and O. Gajanandana, "Development of antibody array for
simultaneous detection of foodborne pathogens," Biosensors and
Bioelectronics, Vol. 24, 2009, pp. 1641-1648.
[13] N. Bojorge Ramírez, A.M. Salgado, and B. Valdman, "The Evolution
and Developments Sensors for Health and Environmental Monitoring:
Problems and Perspectives," Brazilian Journal of Chemical
Engineering, Vol. 26 No. 2, 2008, pp. 227-249.
[14] Y. Jung, J.Y. Jeong, and B.H. Chung, "Recent Advances in
Immobilization Methods of Antibodies on Solid Supports," Analyst, Vol.
133, 2008, pp. 697-701.
[15] E.W. Olle, J. Messamore, M.P. Deogracias, S.D. McClintock, T.D.
Anderson, and K.J. Johnson, "Comparison of Antibody Array Substrates
and the Use of Glycerol to Normalize Spot Morphology," Experimental
and Molecular Pathology, Vol. 79, 2005, pp. 206 - 209.
[16] S.L. Seurynck-Servoss, C.L. Baird, K.D. Rodland, and R.C. Zangar,
"Surface Chemistries for Antibody Microarrays," Frontiers in
bioscience, Vol. 12, 2007, pp. 3956-3964.
[17] E.W. Olle, M.P. Deogracias, J.E. Messamore, S.D. McClintock, A.G.
Barron, T.D. Anderson, and K.J. Johnson, 2007, "Screening of Serum
Samples from Wegener-s Granulomatosis Patients Using Antibody
Microarrays," Proteomics clinical application, Vol. 1, pp. 1212-1220.
[18] M.G.R. Rajan, and B. Gupta, "Immobilization of Antibody Spots on
Glass Surfaces - Potential Use as Antibody Chips for Multianalyte
Assays," BARC Newsletter, Vol. 10 No.237, 2003, pp. 107-110.
[19] D. Maraldo, and R. Mutharasan, "Optimization of Antibody
Immobilization for Sensing Using Piezoelectrically Excited-Millimeter-
Sized Cantilever (Pemc) Sensors," Sensors and Actuators B, Vol. 123,
2007, pp. 474-479.
[20] G. Lonnemann, D. Novick, M. Rubinstein and C. Dinarello,
"Interleukin-18, Interleukin-18 Binding Protein and Impaired Production
of Interferon-Gamma in Chronic Renal Failure," Clinical Nephrology,
Vol. 60 No.5, 2003, pp. 327-334.
[21] C.R. Parikh, A. Janib, J. Mishra, Q. Ma, C. Kelly, J. Barasch, C.L.
Edelstein, and P. Devarajan, "Urine Ngal and Il-18 Are Predictive
Biomarkers for Delayed Graft Function Following Kidney
Transplantation," American Journal of Transplantation, Vol. 6, 2006,
pp. 1639-1645.
[22] C.R. Parikh, J. Mishra, H. Thiessen-Philbrook, B. Dursun, Q. Ma, C.
Kelly, C. Dent, P. Devarajan and C. Edelstein, "Urinary Il-18 Is an Early
Predictive Biomarker of Acute Kidney Injury after Cardiac Surgery,"
Kidney International, Vol. 70, 2006, pp. 199-203.
[23] D. Hu, X. Liu, S. Chen and C. Bao, "Expressions of Il-18 and Its
Binding Protein in Peripheral Blood Leukocytes and Kidney Tissues of
Lupus Nephritis Patients," Clinical Rheumatol, Vol. 29, 2010, pp. 717-
721.
[24] B. DeSilva, W. Smith, R. Weiner, M. Kelley, J. Smolec, B. Lee, M.
Khan, R. Tacey, H. Hill and A. Celniker, "Recommendations for the
bioanalytical method validationof ligand-binding assays to support
pharmacokinetic assessments of macromolecules," Pharmaceutical
Research, Vol. 20, 2003, pp. 1885-1900.
[25] M. Bonham and S. Miller, "Clinical Comparison of 99th Percentile and
10% Coefficient of Variation Cutoff Values for Four Commercially
Available Troponin I Assays," LABMEDICINE, vol. 4o No.8, 2009, pp.
470-473.
@article{"International Journal of Medical, Medicine and Health Sciences:56659", author = "T. Bovornvirakit and K. Viravaidya", title = "Development of an Immunoassay Platform for Diagnosis of Acute Kidney Injury", abstract = "Acute kidney injury (AKI) is a new worldwide public
health problem. A diagnosis of this disease using creatinine is still a
problem in clinical practice. Therefore, a measurement of biomarkers
responsible for AKI has received much attention in the past couple
years. Cytokine interleukin-18 (IL-18) was reported as one of the
early biomarkers for AKI. The most commonly used method to
detect this biomarker is an immunoassay. This study used a planar
platform to perform an immunoassay using fluorescence for
detection. In this study, anti-IL-18 antibody was immobilized onto a
microscope slide using a covalent binding method. Make-up samples
were diluted at the concentration between 10 to 1000 pg/ml to create
a calibration curve. The precision of the system was determined
using a coefficient of variability (CV), which was found to be less
than 10%. The performance of this immunoassay system was
compared with the measurement from ELISA.", keywords = "Acute kidney injury, Acute renal failure, Antibody
immobilization, Interleukin-18", volume = "5", number = "10", pages = "499-5", }
