Abstract: Red blood cells (RBCs) are among the most
commonly and intensively studied type of blood cells in cell biology.
Anemia is a lack of RBCs is characterized by its level compared to
the normal hemoglobin level. In this study, a system based image
processing methodology was developed to localize and extract RBCs
from microscopic images. Also, the machine learning approach is
adopted to classify the localized anemic RBCs images. Several
textural and geometrical features are calculated for each extracted
RBCs. The training set of features was analyzed using principal
component analysis (PCA). With the proposed method, RBCs were
isolated in 4.3secondsfrom an image containing 18 to 27 cells. The
reasons behind using PCA are its low computation complexity and
suitability to find the most discriminating features which can lead to
accurate classification decisions. Our classifier algorithm yielded
accuracy rates of 100%, 99.99%, and 96.50% for K-nearest neighbor
(K-NN) algorithm, support vector machine (SVM), and neural
network RBFNN, respectively. Classification was evaluated in highly
sensitivity, specificity, and kappa statistical parameters. In
conclusion, the classification results were obtained within short time
period, and the results became better when PCA was used.
Abstract: Microorganisms can be removed, inhibited or killed by physical agents, physical processes or chemical agents but they have their inherent disadvantages such as increased resistance against antibiotics etc. Since, plants have endless ability to synthesize aromatic substances which act as the master agents for plant defense mechanisms against microorganisms, insects and herbivores. Thus, secondary metabolites or phytochemicals obtained from plants can be used as agents of disease control nowadays. In the present study effect of different concentrations of acetone fraction of leaves and alcohol fraction of inflorescence of Euphorbia pulcherrima on various cytomorphological parameters i.e. cell number, mycelium width, conidial size, conidiophore size etc. of Aspergillus fumigatus has been studied. Change in mycelium/ hyphal cell width, conidium size, conidiophore size etc. was measured with the help of a previously calibrated oculometer. To study effect on morphology, fungal mycelium along with conidiophore and conidia were stained with cotton blue and mounted in lactophenol and observed microscopically. Inhibitory action of the acetone extract of Euphorbia pulcherrima leaf on growth of Aspergillus fumigatus was investigated. Control containing extract free medium supported profuse growth of the fungus. Although decrease in growth was observed even at 3.95μg/ml but significant inhibition of growth was started at7.81μg/ml concentration of the extract. Complete inhibition was observed at 15.62μg/ml and above. Microscopic examination revealed that at 3.95, 7.81 and 15.62μg/ml extract concentration hyphal cell width was found to be increased from 1.44μm in control to 3.86, 5.24 and 8.98 μm respectively giving a beaded appearance to the mycelium. Vesicle size was reduced from 24.78x20.08μm (control) to 11.34x10.06μm at 3.95μg/ml concentration. At 7.81 and 15.62μg/ml concentration no phialides and sterigmata were observed. Inhibitory action of the alcohol extract of inflorescence on the growth of Aspergillus fumigatus was also studied. Control containing extract free medium supported profuse growth of the fungus. Although decrease in growth was observed even at 3.95μg/ml but complete inhibition was observed at 62.5μg/ml and above. Microscopic examination revealed that hyphal cell width of Aspergillus fumigatus was found to be increased from 1.67μm in control to 5.84μm at MIC i.e. at 62.5μg/ml. Vesicle size was reduced from 44.76x 24.22μm (control) to 11.36x 6.80μm at 15.62μg/ml concentrations. At 31.25 μg/ml and 62.5μg/ml concentration no phialides and sterigmata was found. Spore germination was completely found to be inhibited at 3.95μg/ml concentration. Similarly 92.87% reduction in vesicle size was observed at 15.62μg/ml concentration. It is evident from the results that plant extracts inhibit fungal growth and this inhibition is concentration dependent.
Abstract: Mostly, pedestrian-car accidents occurred at a
signalized interaction is because pedestrians cannot across the
intersection safely within the green light. From the viewpoint of
pedestrian, there might have two reasons. The first one is pedestrians
cannot speed up to across the intersection, such as the elders. The other
reason is pedestrians do not sense that the signal phase is going to
change and their right-of-way is going to lose. Developing signal logic
to protect pedestrian, who is crossing an intersection is the first
purpose of this study. Another purpose of this study is improving the
reliability and reduce delay of public transportation service. Therefore,
bus preemption is also considered in the designed signal logic. In this
study, the traffic data of the intersection of Chong-Qing North Road
and Min-Zu West Road, Taipei, Taiwan, is employed to calibrate and
validate the signal logic by simulation. VISSIM 5.20, which is a
microscopic traffic simulation software, is employed to simulate the
signal logic. From the simulated results, the signal logic presented in
this study can protect pedestrians crossing the intersection
successfully. The design of bus preemption can reduce the average
delay. However, the pedestrian safety and bus preemptive signal will
influence the average delay of cars largely. Thus, whether applying the
pedestrian safety and bus preemption signal logic to an isolated
intersection or not should be evaluated carefully.
Abstract: Microscopic emission and fuel consumption models
have been widely recognized as an effective method to quantify real
traffic emission and energy consumption when they are applied with
microscopic traffic simulation models. This paper presents a
framework for developing the Microscopic Emission (HC, CO, NOx,
and CO2) and Fuel consumption (MEF) models for light-duty
vehicles. The variable of composite acceleration is introduced into
the MEF model with the purpose of capturing the effects of historical
accelerations interacting with current speed on emission and fuel
consumption. The MEF model is calibrated by multivariate
least-squares method for two types of light-duty vehicle using
on-board data collected in Beijing, China by a Portable Emission
Measurement System (PEMS). The instantaneous validation results
shows the MEF model performs better with lower Mean Absolute
Percentage Error (MAPE) compared to other two models. Moreover,
the aggregate validation results tells the MEF model produces
reasonable estimations compared to actual measurements with
prediction errors within 12%, 10%, 19%, and 9% for HC, CO, NOx
emissions and fuel consumption, respectively.
Abstract: Microplasma welding is a less expensive alternative to
laser welding in dental technology. The aim of the study was to
highlight discontinuities present in the microplasma welded joints of
dental base metal alloys by visual analysis. Five base metal alloys
designated for fixed prostheses manufacture were selected for the
experiments. Using these plates, preliminary tests were conducted by
microplasma welding in butt joint configuration, without filler
material, bilaterally and with filler material, proper for each base
metal. Macroscopic visual inspection was performed to assess
carefully the irregularities in the welds. Electron microscopy allowed
detection of discontinuities that are not visible to the eye and
revealing details regarding location, trajectory, morphology and size
of discontinuities. Supplementing visual control with microscopic
analysis allows to detect small discontinuities, which escapes the
macroscopic control and to make a detailed study of the weld.
Abstract: The paper describes a knowledge based system for
analysis of microscopic wear particles. Wear particles contained in
lubricating oil carry important information concerning machine
condition, in particular the state of wear. Experts (Tribologists) in the
field extract this information to monitor the operation of the machine
and ensure safety, efficiency, quality, productivity, and economy of
operation. This procedure is not always objective and it can also be
expensive. The aim is to classify these particles according to their
morphological attributes of size, shape, edge detail, thickness ratio,
color, and texture, and by using this classification thereby predict
wear failure modes in engines and other machinery. The attribute
knowledge links human expertise to the devised Knowledge Based
Wear Particle Analysis System (KBWPAS). The system provides an
automated and systematic approach to wear particle identification
which is linked directly to wear processes and modes that occur in
machinery. This brings consistency in wear judgment prediction
which leads to standardization and also less dependence on
Tribologists.
Abstract: Thermally insulating ceramic coatings also known as
thermal barrier coatings (TBCs) have been essential technologies to
improve the performance and efficiency of advanced gas turbines in
service at extremely high temperatures. The damage mechanisms of
air-plasma sprayed YSZ thermal barrier coatings (TBC) with various
microstructures were studied by microscopic techniques after thermal
cycling. The typical degradation of plasma TBCs that occurs during
cyclic furnace testing of an YSZ and alumina coating on a Titanium
alloy are analyzed. During the present investigation the effects of
topcoat thickness, bond coat oxidation, thermal cycle lengths and test
temperature are investigated using thermal cycling. These results
were correlated with stresses measured by a spectroscopic technique
in order to understand specific damage mechanism. The failure
mechanism of former bond coats was found to involve fracture
initiation at the thermally grown oxide (TGO) interface and at the
TGO bond coat interface. The failure mechanism of the YZ was
found to involve combination of fracture along the interface between
TGO and bond coat.
Abstract: Earthmoving operations are a major part of many
construction projects. Because of the complexity and fast-changing
environment of such operations, the planning and estimating are
crucial on both planning and operational levels. This paper presents
the framework ofa microscopic discrete-event simulation system for
modeling earthmoving operations and conducting productivity
estimations on an operational level.A prototype has been developed
to demonstrate the applicability of the proposed framework, and this
simulation system is presented via a case study based on an actual
earthmoving project. The case study shows that the proposed
simulation model is capable of evaluating alternative operating
strategies and resource utilization at a very detailed level.
Abstract: In this article, biomechanical aspects of hen-s eggshell as a natural ceramic structure are studied. The images, taken by a scanning electron microscope (SEM), are used to investigate the microscopic aspects of the egg. It is observed that eggshell has a three-layered microstructure with different morphological and structural characteristics. Studies on the eggshell membrane (ESM) as a prosperous tissue suggest that it is placed to prevent the penetration of microorganisms into the egg. Finally, numerical models of the egg are presented to study the stress distribution and its deformation under different loading conditions. The effects of two different types of loading (hydrostatic and point loadings) on two different shell models (with constant and variable thicknesses) are investigated in detail.
Abstract: Now a days, a significant part of commercial and governmental organisations like museums, cultural organizations, libraries, commercial enterprises, etc. invest intensively in new technologies for image digitization, digital libraries, image archiving and retrieval. Hence image authorization, authentication and security has become prime need. In this paper, we present a semi-fragile watermarking scheme for color images. The method converts the host image into YIQ color space followed by application of orthogonal dual domains of DCT and DWT transforms. The DCT helps to separate relevant from irrelevant image content to generate silent image features. DWT has excellent spatial localisation to help aid in spatial tamper characterisation. Thus image adaptive watermark is generated based of image features which allows the sharp detection of microscopic changes to locate modifications in the image. Further, the scheme utilises the multipurpose watermark consisting of soft authenticator watermark and chrominance watermark. Which has been proved fragile to some predefined processing like intentinal fabrication of the image or forgery and robust to other incidental attacks caused in the communication channel.
Abstract: Vinegar or sour wine is a product of alcoholic and
subsequent acetous fermentation of sugary precursors derived from
several fruits or starchy substrates. This delicious food additive and
supplement contains not less than 4 grams of acetic acid in 100 cubic
centimeters at 20°C. Among the large number of bacteria that are
able to produce acetic acid, only few genera are used in vinegar
industry most significant of which are Acetobacter and
Gluconobacter. In this research we isolated and identified an
Acetobacter strain from Iranian apricot, a very delicious and sensitive
summer fruit to decay, we gathered from fruit's stores in Isfahan,
Iran. The main culture media we used were Carr, GYC, Frateur and
an industrial medium for vinegar production. We isolated this strain
using a novel miniature fermentor we made at Pars Yeema
Biotechnologists Co., Isfahan Science and Technology Town (ISTT),
Isfahan, Iran. The microscopic examinations of isolated strain from
Iranian apricot showed gram negative rods to cocobacilli. Their
catalase reaction was positive and oxidase reaction was negative and
could ferment ethanol to acetic acid. Also it showed an acceptable
growth in 5%, 7% and 9% ethanol concentrations at 30°C using
modified Carr media after 24, 48 and 96 hours incubation
respectively. According to its tolerance against high concentrations of
ethanol after four days incubation and its high acetic acid production,
8.53%, after 144 hours, this strain could be considered as a suitable
industrial strain for a production of a new type of vinegar, apricot
vinegar, with a new and delicious taste. In conclusion this is the first
report of isolation and identification of an Acetobacter strain from
Iranian apricot with a very good tolerance against high ethanol
concentrations as well as high acetic acid productivity in an
acceptable incubation period of time industrially. This strain could be
used in vinegar industry to convert apricot spoilage to a beneficiary
product and mentioned characteristics have made it as an amenable
strain in food and agricultural biotechnology.
Abstract: The present research focus on the processing of mullite-based ceramics from oil refinery industrial wastes and byproducts of agricultural industry and on the investigating of silane modified surface of ceramics. Two waste products were used as initial material – waste aluminum oxide and waste rice husk. The burning - out additives used were waste rise husk. It is known that the oxide ceramics surface is hydrophilic due to the presence of – OH groups in it. The nature of ceramic surface regarding permeation of water and hydrocarbons can be changed by further treatment with silanes. The samples were studied mainly by X-ray analysis, FT-IR absorbance measurements and microscopic analysis. The X-ray analyses showed the phase composition depends on the firing temperature and on the purity of the starting alumina. Two kind of silanes were used for the transformation of surface from hydrophilic to hydrophobic – trimethoxymethylsilane (TMMS) and trimethylclorsilane (TMCS).
Abstract: This study was aimed for investigating of
manufacturing high aluminum content Mg alloys using a horizontal
twin roll caster. Recently, weight saving has been key issues for lighter
transport equipments as well as electronic component parts. As
alternative materials to aluminum alloys, developing magnesium alloy
with higher strength has been expected. Normally high Aluminum
content Mg alloy has poor ductility and is difficult to be rolled because
of its high strength. However, twin roll casting process is suitable for
manufacturing wrought Mg alloys because materials can be cast
directly from molten metal. In this study, manufacturing of high
aluminum content magnesium alloy sheet using the roll casting
process has been carried out. Effects of manufacturing parameter, such
as roll velocity, pouring temperature and roll gap, on casting was
investigated. A microscopic observation of the crystals of cross section
of as cast strip as well as rolled strip was conducted.
Abstract: In this paper we propose a new traffic simulation
package, TDMSim, which supports both macroscopic and
microscopic simulation on free-flowing and regulated traffic systems.
Both simulators are based on travel demands, which specify the
numbers of vehicles departing from origins to arrive at different
destinations. The microscopic simulator implements the carfollowing
model given the pre-defined routes of the vehicles but also
supports the rerouting of vehicles. We also propose a macroscopic
simulator which is built in integration with the microscopic simulator
to allow the simulation to be scaled for larger networks without
sacrificing the precision achievable through the microscopic
simulator. The macroscopic simulator also enables the reuse of
previous simulation results when simulating traffic on the same
networks at later time. Validations have been conducted to show the
correctness of both simulators.
Abstract: The comparative analysis of different taxonomic
groups of microorganisms isolated from dark chernozem soils under
different agricultures (alfalfa, melilot, sainfoin, soybean, rapeseed) at
Almaty region of Kazakhstan was conducted. It was shown that the
greatest number of micromycetes was typical to the soil planted with
alfalfa and canola. Species diversity of micromycetes markedly
decreases as it approaches the surface of the root, so that the species
composition in the rhizosphere is much more uniform than in the
virgin soil. Promising strains of microscopic fungi and yeast with
plant growth-promoting activity to agricultures were selected. Among
the selected fungi there are representatives of Penicillium bilaiae,
Trichoderma koningii, Fusarium equiseti, Aspergillus ustus. The
highest rates of growth and development of seedlings of plants
observed under the influence of yeasts Aureobasidium pullulans,
Rhodotorula mucilaginosa, Metschnikovia pulcherrima. Using
molecular - genetic techniques confirmation of the identification
results of selected micromycetes was conducted.
Abstract: the elastic scattering of protons, deuterons and 3He on 6Li at different incident energies have been analyzed in the framework of the optical model using ECIS88 as well as SPI GENOA codes. The potential parameters were extracted in the phenomenological treatment of measured by us angular distributions and literature data. A good agreement between theoretical and experimental differential cross sections was obtained in whole angular range. Parameters for real part of potential have been also calculated microscopically with singleand double-folding model for the p and d, 3He scattering, respectively, using DFPOT code. For best agreement with experiment the normalization factor N for the potential depth is obtained in the range of 0.7-0.9.
Abstract: Image mosaicing techniques are usually employed to offer researchers a wider field of view of microscopic image of biological samples. a mosaic is commonly achieved using automated microscopes and often with one “color" channel, whether it refers to natural or fluorescent analysis. In this work we present a method to achieve three subsequent mosaics of the same part of a stem cell culture analyzed in phase contrast and in fluorescence, with a common non-automated inverted microscope. The mosaics obtained are then merged together to mark, in the original contrast phase images, nuclei and cytoplasm of the cells referring to a mosaic of the culture, rather than to single images. The experiments carried out prove the effectiveness of our approach with cultures of cells stained with calcein (green/cytoplasm and nuclei) and hoechst (blue/nuclei) probes.
Abstract: In this study acoustic emission (AE) signals obtained during deformation and fracture of two types of ferrite-martensite dual phase steels (DPS) specimens have been analyzed in frequency domain. For this reason two low carbon steels with various amounts of carbon were chosen, and intercritically heat treated. In the introduced method, identifying the mechanisms of failure in the various phases of DPS is done. For this aim, AE monitoring has been used during tensile test of several DPS with various volume fraction of the martensite (VM) and attempted to relate the AE signals and failure mechanisms in these steels. Different signals, which referred to 2-3 micro-mechanisms of failure due to amount of carbon and also VM have been seen. By Fast Fourier Transformation (FFT) of signals in distinct locations, an excellent relationship between peak frequencies in these areas and micro-mechanisms of failure were seen. The results were verified by microscopic observations (SEM).
Abstract: Nanoemulsions are a class of emulsions with a droplet
size in the range of 50–500 nm and have attracted a great deal of
attention in recent years because it is unique characteristics. The
physicochemical properties of nanoemulsion suggests that it can be
successfully used to recover the residual oil which is trapped in the
fine pore of reservoir rock by capillary forces after primary and
secondary recovery. Oil-in-water nanoemulsion which can be formed
by high-energy emulsification techniques using specific surfactants
can reduce oil-water interfacial tension (IFT) by 3-4 orders of
magnitude. The present work is aimed on characterization of oil-inwater
nanoemulsion in terms of its phase behavior, morphological
studies; interfacial energy; ability to reduce the interfacial tension and
understanding the mechanisms of mobilization and displacement of
entrapped oil blobs by lowering interfacial tension both at the
macroscopic and microscopic level. In order to investigate the
efficiency of oil-water nanoemulsion in enhanced oil recovery
(EOR), experiments were performed to characterize the emulsion in
terms of their physicochemical properties and size distribution of the
dispersed oil droplet in water phase. Synthetic mineral oil and a series
of surfactants were used to prepare oil-in-water emulsions.
Characterization of emulsion shows that it follows pseudo-plastic
behaviour and drop size of dispersed oil phase follows lognormal
distribution. Flooding experiments were also carried out in a
sandpack system to evaluate the effectiveness of the nanoemulsion as
displacing fluid for enhanced oil recovery. Substantial additional
recoveries (more than 25% of original oil in place) over conventional
water flooding were obtained in the present investigation.
Abstract: Thirty six samples from each (aerobic and anoxic)
activated sludge were collected from two wastewater treatment plants
with MBRs in Berlin, Germany. The samples were prepared for count
and definition of fungal isolates; these isolates were purified by
conventional techniques and identified by microscopic examination.
Sixty tow species belonging to 28 genera were isolated from
activated sludge samples under aerobic conditions (28 genera and 58
species) and anoxic conditions (26 genera and 52 species). The
obtained data show that, Aspergillus was found at 94.4% followed by
Penicillium 61.1 %, Fusarium (61.1 %), Trichoderma (44.4 %) and
Geotrichum candidum (41.6 %) species were the most prevalent in all
activated sludge samples. The study confirmed that fungi can thrive
in activated sludge and sporulation, but isolated in different numbers
depending on the effect of aeration system. Some fungal species in
our study are saprophytic, and other a pathogenic to plants and
animals.