Abstract: The soil ecology of the organic and mineral soil layers
of laurel-leaved and Cryptomeria japonica forest in the Kasuga-yama
Hill Primeval Forest (Nara, Japan) was assessed. The number of
bacteria obtained by the dilution plate count method was less than
0.05% of those counted by the direct microscopic count. We therefore
found that forest soil contains large numbers of non-culturable
bacteria compared with agricultural soils. The numbers of bacteria and
fungi obtained by both the dilution plate count and the direct
microscopic count were larger in the deeper horizons (F and H) of the
organic layer than in the mineral soil layer. This suggests that active
microbial metabolism takes place in the organic layer. The numbers of
bacteria and the length of fungal hyphae obtained by the direct count
method were greater in the H horizon than in the F horizon. The direct
microscopic count revealed numerous non-culturable bacteria and
fungi in the soil. The ratio of fungal to bacterial biomass was lower in
the laurel-leaved forest soil. The fungal biomass was therefore
relatively low in the laurel-leaved forest soil due to differences in
forest vegetation.
Abstract: Grey mold on grape is caused by the fungus Botrytis
cinerea Pers. Trichodex WP, a new biofungicide, that contains fungal
spores of Trichoderma harzianum Rifai, was used for biological
control of Grey mold on grape. The efficacy of Trichodex WP has
been reported from many experiments. Experiments were carried out
in the locality – Banatski Karlovac, on grapevine species – talijanski
rizling. The trials were set according to instructions of methods
PP1/152(2) and PP1/17(3) , according to a fully randomized block
design. Phytotoxicity was estimated by PP methods 1/135(2), the
intensity of infection according to Towsend Heuberger , the
efficiency by Abbott, the analysis of variance with Duncan test and
PP/181(2). Application of Trichodex WP is limited to the first two
treatments. Other treatments are performed with the fungicides based
on a.i. procymidone, vinclozoline and iprodione.
Abstract: The field research was carried out at the Látókép AGTC KIT research area of the University of Debrecen in Eastern-Hungary, on the area of the aeolain loess of the Hajdúság. We examined the effects of the sowing time on the phytopathogenic characteristics and yield production by applying various fertilizer treatments on two different sunflower genotypes (NK Ferti, PR64H42) in 2012 and 2013. We applied three different sowing times (early, optimal, late) and two different treatment levels of fungicides (control = no fungicides applied, double fungicide protection).
During our investigations, the studied cropyears were of different sowing time optimum in terms of yield amount (2012: early, 2013: average). By Pearson’s correlation analysis, we have found that delaying the sowing time pronouncedly decreased the extent of infection in both crop years (Diaporthe: r=0.663**, r=0.681**, Sclerotinia: r=0.465**, r=0.622**). The fungicide treatment not only decreased the extent of infection, but had yield increasing effect too (2012: r=0.498**, 2013: r=0.603**). In 2012, delaying of the sowing time increased (r=0.600**), but in 2013, it decreased (r= 0.356*) the yield amount.
Abstract: Empty Fruit Bunches (EFB) and Palm Oil Mill
Effluent (POME) are two main wastes from oil palm industries which
contain rich lignocellulose. Degradation of EFB and POME by
microorganisms will produce hydrolytic enzyme which will degrade
cellulose and hemicellulose during composting process. However,
normal composting takes about four to six months to reach maturity.
Hence, application of fungi into compost can shorten the period of
composting. This study identifies the effect of xylanase and cellulase
produced by Aspergillus niger and Trichoderma virens on
composting process using EFB and POME. The degradation of EFB
and POME indicates the lignocellulolytic capacity of Aspergillus
niger and Trichoderma virens with more than 7% decrease in
hemicellulose and more than 25% decrease in cellulose for both
inoculated compost. Inoculation of Aspergillus niger and
Trichoderma virens also increased the enzyme activities during the
composting period compared to the control compost by 21% for both
xylanase and cellulase. Rapid rise in the activities of cellulase and
xylanase was observed by Aspergillus niger with the highest
activities of 14.41 FPU/mg and 3.89 IU/mg, respectively. Increased
activities of cellulase and xylanase also occurred in inoculation of
Trichoderma virens with the highest activities obtained at 13.21
FPU/mg and 4.43 IU/mg, respectively. Therefore, it is evident that
the inoculation of fungi can increase the enzyme activities hence
effectively degrading the EFB and POME.
Abstract: White rust, caused by Albugo candida, is the most
destructive foliar diseases of persian cress, Lepidium sativum in Iran.
Application of fungicide is the most common method for the disease
control. However, regarding the problems created by synthetic
pesticides application, environmentally safe methods are needed to
replace chemical pesticides. In this study, the antifungal activity of
plant natural extracts was investigated for their ability to inhibit
zoospore release from sporangia of A. candida. The crude extract of
46 plants was obtained using methanol. The inhibitory effect of the
extracts was examined by mixing the plant extracts with a
zoosporangial suspension of A. candida (1×106 spore/ml) at three
concentrations, 250, 100 and 50 ppm. The experiments were
conducted in a completely randomized design, with three replicates.
The results of the experiment showed that three out of 46 plants
species, including, Rhus coriaria, Anagallis arvensis and Mespilus
germanica were completely inhibit zoospore release from
zoosporangia of Albugo candida at concentration of 50 ppm.
Abstract: In recent years application of natural antimicrobials
instead of conventional ones, due to their hazardous effects on health,
has got serious attentions. On the basis of the results of different
studies, chitosan, a natural bio-degradable and non-toxic
biopolysaccharide derived from chitin, has potential to be used as a
natural antimicrobial. Chitosan has exhibited high antimicrobial
activity against a wide variety of pathogenic and spoilage
microorganisms, including fungi, and Gram-positive and Gramnegative
bacteria. The antimicrobial action is influenced by intrinsic
factors such as the type of chitosan, the degree of chitosan
polymerization and extrinsic factors such as the microbial organism,
the environmental conditions and presence of the other components.
The use of chitosan in food systems should be based on sufficient
knowledge of the complex mechanisms of its antimicrobial mode of
action. In this article we review a number of studies on the
investigation of chitosan antimicrobial properties and application of
them in culture and food mediums.
Abstract: A three-year field experiment (2010-2012) was
conducted to determine the abundance of epiphytic and endophytic
filamentous fungi colonizing the grain of winter wheat cv. Bogatka.
Wheat spikes were protected with tebuconazole or chitosan at the
watery ripe stage. Untreated plants served as control. Tebuconazole
exerted an inhibitory effect primarily on F. culmorum and F.
graminearum, and its effectiveness was determined by the pressure
from pathogens that infected wheat spikes during the growing season.
Chitosan did not suppress the growth of Fusarium species and
Alternaria alternata.
Abstract: Prior to 1975, women in Laos suffered from having
reduced levels of power over decision-making in their families and in
their communities. This has had a negative impact on their ability to
develop their own identities. Their roles were identified as being
responsible for household activities and making preparations for their
marriage. Many women lost opportunities to get educated and access
the outdoor work that might have empowered them to improve their
situations. So far, no accurate figures of either emigrants or return
migrants have been compiled but it appears that most of them were
women, and it was women who most and more frequently remitted
money home. However, very few recent studies have addressed the
relationship between remittances and the roles of women in Laos.
This study, therefore, aims at redressing to some extent the
deficiencies in knowledge. Qualitative techniques were used to gather
data, including individual in-depth interviews and direct observation
in combination with the content analysis method. Forty women in
Vientiane Municipality and Savannakhet province were individually
interviewed. It was found that the monetary remittance was typically
used for family security and well-being; on fungible activities; on
economic and business activities; and on community development,
especially concerning hospitality and providing daily household
necessities. Remittances played important roles in improving many
respondents- livelihoods and positively changed their identities in
families and communities. Women became empowered as they were
able to start commercial businesses, rather than taking care of (just)
housework, children and elders. Interviews indicated that 92.5% of
the respondents their quality of lives improved, 90% felt happier in
their families and 82.5% felt conflicts in their families were reduced.
Abstract: Eukaryotic protein-coding genes are interrupted by spliceosomal introns, which are removed from the RNA transcripts before translation into a protein. The exon-intron structures of different eukaryotic species are quite different from each other, and the evolution of such structures raises many questions. We try to address some of these questions using statistical analysis of whole genomes. We go through all the protein-coding genes in a genome and study correlations between the net length of all the exons in a gene, the number of the exons, and the average length of an exon. We also take average values of these features for each chromosome and study correlations between those averages on the chromosomal level. Our data show universal features of exon-intron structures common to animals, plants, and protists (specifically, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Cryptococcus neoformans, Homo sapiens, Mus musculus, Oryza sativa, and Plasmodium falciparum). We have verified linear correlation between the number of exons in a gene and the length of a protein coded by the gene, while the protein length increases in proportion to the number of exons. On the other hand, the average length of an exon always decreases with the number of exons. Finally, chromosome clustering based on average chromosome properties and parameters of linear regression between the number of exons in a gene and the net length of those exons demonstrates that these average chromosome properties are genome-specific features.
Abstract: This paper reports optimization of characteristics of bioballistic transformation of spring soft wheat (Triticum aestivum L. cultivar Raduga) and getting of transgenic plants, carrying pea lectin gene. This gene will let to create new associative wheat symbiosis with nodule bacteria of field pea, which has growth encouraging, fungistatic and other useful characteristics.
Abstract: The spores of entomopathogenic fungi, Beauveria bassiana was evaluated for their compatibility with four surfactants; SDS (sodium dodyl sulphate) and CABS-65 (calcium alkyl benzene sulphonate), Tween 20 (polyethylene sorbitan monolaureate) and Tween 80 (polyoxyethylene sorbitan monoleate) at six different concentrations (0.1%, 0.5%, 1%, 2.5%, 5% and 10%). Incubated spores showed decrease in concentrations due to conversion of spores to hyphae. The maximum germination recorded in 72 h incubated spores varied with surfactant concentration at 49-68% (SDS), 39- 53% (CABS), 78-92% (Tween 80) and 80-92% (Tween 20), while the optimal surfactant concentration for spore germination was found to be 2.5-5%. The surfactant effect on spores was more pronounced with SDS and CABS-65, where significant deterioration and loss in viability of the incubated spores was observed. The effect of Tween 20 and Tween 80 were comparatively less inhibiting. The results of the study would help in surfactant selection for B. bassiana emulsion preparation.
Abstract: This current research focused on development of degradable starch based packaging film with enhanced mechanical properties. A series of low density polyethylene (LDPE)/tapioca starch compounds with various tapioca starch contents were prepared by twin screw extrusion with the addition of maleic anhydride grafted polyethylene as compatibilizer. Palm cooking oil was used as processing aid to ease the blown film process, thus, degradable film can be processed via conventional blown film machine. Studies on their characteristics, mechanical properties and biodegradation were carried out by Fourier Transform Infrared (FTIR) spectroscopy and optical properties, tensile test and exposure to fungi environment respectively. The presence of high starch contents had an adverse effect on the tensile properties of LDPE/tapioca starch blends. However, the addition of compatibilizer to the blends improved the interfacial adhesion between the two materials, hence, improved the tensile properties of the films. High content of starch amount also was found to increase the rate of biodegradability of LDPE/tapioca starch films. It can be proved by exposure of the film to fungi environment. A growth of microbes colony can be seen on the surface of LDPE/tapioca starch film indicates that the granular starch present on the surface of the polymer film is attacked by microorganisms, until most of it is assimilated as a carbon source.
Abstract: This study was set to determine the antimicrobial
activities of brine salting, chlorinated solution, and oil frying
treatments on enteric bacteria and fungi in Rastrineobola argentea
fish from fish landing beaches within L. Victoria basin of western
Kenya. Statistical differences in effectiveness of the different
treatment methods was determined by single factor ANOVA, and
paired two-tail t-Test was performed to compare the differences in
moisture contents before and after storage. Oil fried fish recorded the
lowest microbial loads, sodium chloride at 10% concentration was
the second most effective and chlorinated solution even at 150ppm
was the least effective against the bacteria and fungi in fish. Moisture
contents of the control and treated fish were significantly lower after
storage. These results show that oil frying of fish should be adopted
for processing and preserving Rastrineobola argentea which is the
most abundant and affordable fish species from Lake Victoria.
Abstract: Lignocellulosic materials are considered the most
abundant renewable resource available for the Bioethanol
Production. Water Hyacinth is one of potential raw material of the
world-s worst aquatic plant as a feedstock to produce Bioethanol.
The purposed this research is obtain reduced of matter for
biodegradation lignin in Biological pretreatment with White Rot
Fungi eg. Phanerochaete Chrysosporium using Solid state
Fermentation methods. Phanerochaete Chrysosporium is known to
have the best ability to degraded lignin, but simultaneously it can also
degraded cellulose and hemicelulose. During 8 weeks incubation,
water hyacinth occurred loss of weight reached 34,67%, while loss
of lignin reached 67,21%, loss of cellulose reached 11,01% and loss
of hemicellulose reached 36,56%. The kinetic of losses lignin using
regression linear plot, the results is obtained constant rate (k) of
reduction lignin is -0.1053 and the equation of reduction of lignin
is y = wo - 0, 1.53 x
Abstract: The objective of this study is to evaluate the
occurrence of fungi in aerobic and anoxic activated sludge from
membrane bioreactors (MBRs). Thirty-six samples of both aerobic
and anoxic activated sludge were taken from 2 MBR treating
domestic wastewater. Over a period of eight months 2 samples from
each plant were taken per month. The samples were prepared for
count and definition of fungi. The obtained data show that, sixty
species belonging to 27 genera were collected from activated sludge
samples under aerobic and anoxic conditions. Regarding to the fungi
definition, under aerobic condition the Geotrichum was found at
(8.8%) followed by Penicillium (75.0%), Yeasts (65.7%) and
Trichoderma (55.5%), while Yeasts (77.1%) Geotrichum
candidumand Penicillium (61.1%) species were the most prevalent in
anoxic activated sludge. The results indicate that activated sludge is
habitat for growth and sporulation of different groups of fungi, both
saprophytic and pathogenic.
Abstract: In this study, the ability of Aspergillus niger and
Penicillium simplicissimum to extract heavy metals from a spent
refinery catalyst was investigated. For the first step, a spent
processing catalyst from one of the oil refineries in Iran was
physically and chemically characterized. Aspergillus niger and
Penicillium simplicissimum were used to mobilize Al/Co/Mo/Ni from
hazardous spent catalysts. The fungi were adapted to the mixture of
metals at 100-800 mg L-1 with increments in concentration of 100 mg
L-1. Bioleaching experiments were carried out in batch cultures. To
investigate the production of organic acids in sucrose medium,
analyses of the culture medium by HPLC were performed at specific
time intervals after inoculation. The results obtained from Inductive
coupled plasma-optical emission spectrometry (ICP-OES) showed
that after the one-step bioleaching process using Aspergillus niger,
maximum removal efficiencies of 27%, 66%, 62% and 38% were
achieved for Al, Co, Mo and Ni, respectively. However, the highest
removal efficiencies using Penicillium simplicissimum were of 32%,
67%, 65% and 38% for Al, Co, Mo and Ni, respectively
Abstract: The present study has been taken to explore the
screening of in vitro antimicrobial activities of D-galactose-binding
sponge lectin (HOL-30). HOL-30 was purified from the marine
demosponge Halichondria okadai by affinity chromatography. The
molecular mass of the lectin was determined to be 30 kDa with a
single polypeptide by SDS-PAGE under non-reducing and reducing
conditions. HOL-30 agglutinated trypsinized and glutaraldehydefixed
rabbit and human erythrocytes with preference for type O
erythrocytes. The lectin was subjected to evaluation for inhibition of
microbial growth by the disc diffusion method against eleven human
pathogenic gram-positive and gram-negative bacteria. The lectin
exhibited strong antibacterial activity against gram-positive bacteria,
such as Bacillus megaterium and Bacillus subtilis. However, it did
not affect against gram-negative bacteria such as Salmonella typhi
and Escherichia coli. The largest zone of inhibition was recorded of
Bacillus megaterium (12 in diameter) and Bacillus subtilis (10 mm in
diameter) at a concentration of the lectin (250 μg/disc). On the other
hand, the antifungal activity of the lectin was investigated against six
phytopathogenic fungi based on food poisoning technique. The lectin
has shown maximum inhibition (22.83%) of mycelial growth of
Botrydiplodia theobromae at a concentration of 100 μg/mL media.
These findings indicate that the lectin may be of importance to
clinical microbiology and have therapeutic applications.
Abstract: Auckland has a temperate climate with comfortable warm, dry summers and mild, wet winters. Auckland house design not only focus on winter thermal performance and indoor thermal condition, but also indoor moisture control, which is closely related to indirect health effects such as dust mites, fungi, etc. Most Auckland houses are designed to use temporary heating for winter indoor thermal comfort. Based on field study data of indoor microclimate conditions of two Auckland townhouses with a whole home mechanical ventilation system or a passive wind directional skylight vent, this study is to evaluate and compare indoor moisture conditions of two insulated townhouses only using temporary heating with different ventilation systems.